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The map label for this gene is rpoN [H]

Identifier: 77457084

GI number: 77457084

Start: 1005472

End: 1006965

Strand: Reverse

Name: rpoN [H]

Synonym: Pfl01_0857

Alternate gene names: 77457084

Gene position: 1006965-1005472 (Counterclockwise)

Preceding gene: 77457085

Following gene: 77457083

Centisome position: 15.64

GC content: 59.3

Gene sequence:

>1494_bases
ATGAAACCATCGCTAGTCTTGAGAATGGGCCAGCAGCTGACGATGACACCGCAGCTGCAACAGGCCATCCGCCTGCTCCA
ATTGTCGACCCTGGATCTGCAACAGGAAATCCAGGAGGCTCTGGAATCCAATCCGATGCTCGAACGCCAGGAAGACGGCG
ACGACTTCGACAACTCCGATCCCATGGCCGACAACGCCGAACAGAAGCCCAACACCGAGATCCAGGAACCTTCCTATCAG
GAGACCGCGCCGACAGTCGACAACCTCGAAGACGGCGAATGGAACGAGCGCATCCCCAACGAACTGCCCGTCGACACCGC
TTGGGAAGACGTCTATCAGACCAGCGCCAGCAGCCTGCCGAGCAGCGATGATGACGAGTGGGACTTCACCACCCGCACCT
CCGCCGGCGAGAGCCTGCAAAGTCACCTGCTGTGGCAGTTGAACCTTGCCCCGATGTCCGACACCGATCGCCTGATCGCT
GTCACCCTGATCGATTGCATCAACAATCAGGGTTACCTCGACGAAACCCTCGAAGAAATTCTCGACGCCTTCGATCCGGA
ACTCGACATCGAGCTGGACGAAATCGAAGCCGTCCTGCACCGCATCCAGCAGTTCGAACCGGCCGGCATCGGCGCACGCA
ACCTGGGCGAATGCCTGTTGCTGCAATTGCGCCAGCTGTCGGCCAAGACCCCTTGGCTGGCAGAGGCCAAGCGTCTGGTC
ACCGATTACATCGACCTGCTCGGCAGCCGCGACTACAGCCAGCTGATGCGCCGCATGAAGCTCAAGGAAGACGAACTGCG
CCAAGTCATCGAACTGGTGCAGAGCCTCAACCCGCGCCCCGGCTCTCAGATCGAATCCACCGAAGCCGAATACGTGGTAC
CTGACGTCATCGTACGCAAGGACAACGAGCGCTGGCTGGTCGAGCTGAACCAGGAATCGGTGCCGCGCCTGCGGGTCAAC
GCCCAGTACGCCGGTTTCGTGCGCCGCGCCGACACCAGCGCCGACAACACCTTCATGCGCAATCAGTTGCAGGAAGCCCG
CTGGTTCATCAAGAGCCTGCAGAGCCGCAACGAAACCCTGATGAAAGTAGCGACCCAGATCGTCGAGCACCAGCGCGGCT
TTCTGGAATACGGCGACGAAGCGATGAAGCCGCTGGTCCTGCATGACATCGCTGAAGCGGTGGGCATGCACGAGTCGACG
ATTTCCCGCGTGACCACGCAAAAGTTCATGCATACCCCGCGAGGCATATATGAACTGAAATACTTTTTCTCCAGCCATGT
GAGCACTTCAGAAGGCGGTGAATGCTCGTCCACGGCGATCCGCGCGATCATCAAGAAACTGGTTGCCGCGGAAAATCAGA
AAAAGCCGTTGAGTGACAGCAAGATCGCTGGTTTACTGGAGGCACAAGGCATTCAGGTGGCTCGCCGCACCGTCGCCAAG
TACCGCGAATCCCTCGGGATCGCGCCTTCGAGCGAACGCAAGCGGTTGATGTAA

Upstream 100 bases:

>100_bases
ACACGACAGTTTCAGGCATATAATTTGCTTAAGTTTGGCGCTTCGGCGCCCTGTAGTGGATGGCGCATAGCGCCGGCGAA
TAAGGTGTTAAGCCCCTGCC

Downstream 100 bases:

>100_bases
GCCACGTTACAGCGTTCCAGTGGCAGGCTTTTCGGCCTGCCGCTTTATGCACTGGCAACGAAGGAGAAGCTGTATGCAAG
TCAACATCAGTGGACACCAA

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MKPSLVLRMGQQLTMTPQLQQAIRLLQLSTLDLQQEIQEALESNPMLERQEDGDDFDNSDPMADNAEQKPNTEIQEPSYQ
ETAPTVDNLEDGEWNERIPNELPVDTAWEDVYQTSASSLPSSDDDEWDFTTRTSAGESLQSHLLWQLNLAPMSDTDRLIA
VTLIDCINNQGYLDETLEEILDAFDPELDIELDEIEAVLHRIQQFEPAGIGARNLGECLLLQLRQLSAKTPWLAEAKRLV
TDYIDLLGSRDYSQLMRRMKLKEDELRQVIELVQSLNPRPGSQIESTEAEYVVPDVIVRKDNERWLVELNQESVPRLRVN
AQYAGFVRRADTSADNTFMRNQLQEARWFIKSLQSRNETLMKVATQIVEHQRGFLEYGDEAMKPLVLHDIAEAVGMHEST
ISRVTTQKFMHTPRGIYELKYFFSSHVSTSEGGECSSTAIRAIIKKLVAAENQKKPLSDSKIAGLLEAQGIQVARRTVAK
YRESLGIAPSSERKRLM

Sequences:

>Translated_497_residues
MKPSLVLRMGQQLTMTPQLQQAIRLLQLSTLDLQQEIQEALESNPMLERQEDGDDFDNSDPMADNAEQKPNTEIQEPSYQ
ETAPTVDNLEDGEWNERIPNELPVDTAWEDVYQTSASSLPSSDDDEWDFTTRTSAGESLQSHLLWQLNLAPMSDTDRLIA
VTLIDCINNQGYLDETLEEILDAFDPELDIELDEIEAVLHRIQQFEPAGIGARNLGECLLLQLRQLSAKTPWLAEAKRLV
TDYIDLLGSRDYSQLMRRMKLKEDELRQVIELVQSLNPRPGSQIESTEAEYVVPDVIVRKDNERWLVELNQESVPRLRVN
AQYAGFVRRADTSADNTFMRNQLQEARWFIKSLQSRNETLMKVATQIVEHQRGFLEYGDEAMKPLVLHDIAEAVGMHEST
ISRVTTQKFMHTPRGIYELKYFFSSHVSTSEGGECSSTAIRAIIKKLVAAENQKKPLSDSKIAGLLEAQGIQVARRTVAK
YRESLGIAPSSERKRLM
>Mature_497_residues
MKPSLVLRMGQQLTMTPQLQQAIRLLQLSTLDLQQEIQEALESNPMLERQEDGDDFDNSDPMADNAEQKPNTEIQEPSYQ
ETAPTVDNLEDGEWNERIPNELPVDTAWEDVYQTSASSLPSSDDDEWDFTTRTSAGESLQSHLLWQLNLAPMSDTDRLIA
VTLIDCINNQGYLDETLEEILDAFDPELDIELDEIEAVLHRIQQFEPAGIGARNLGECLLLQLRQLSAKTPWLAEAKRLV
TDYIDLLGSRDYSQLMRRMKLKEDELRQVIELVQSLNPRPGSQIESTEAEYVVPDVIVRKDNERWLVELNQESVPRLRVN
AQYAGFVRRADTSADNTFMRNQLQEARWFIKSLQSRNETLMKVATQIVEHQRGFLEYGDEAMKPLVLHDIAEAVGMHEST
ISRVTTQKFMHTPRGIYELKYFFSSHVSTSEGGECSSTAIRAIIKKLVAAENQKKPLSDSKIAGLLEAQGIQVARRTVAK
YRESLGIAPSSERKRLM

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the xylCAB operon and the xylS gene. The open complex (sigma-54 a

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=499, Percent_Identity=53.7074148296593, Blast_Score=535, Evalue=1e-153,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 56537; Mature: 56537

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPSLVLRMGQQLTMTPQLQQAIRLLQLSTLDLQQEIQEALESNPMLERQEDGDDFDNSD
CCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
PMADNAEQKPNTEIQEPSYQETAPTVDNLEDGEWNERIPNELPVDTAWEDVYQTSASSLP
CCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHCCC
SSDDDEWDFTTRTSAGESLQSHLLWQLNLAPMSDTDRLIAVTLIDCINNQGYLDETLEEI
CCCCCCCCCCCCCCHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHH
LDAFDPELDIELDEIEAVLHRIQQFEPAGIGARNLGECLLLQLRQLSAKTPWLAEAKRLV
HHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
TDYIDLLGSRDYSQLMRRMKLKEDELRQVIELVQSLNPRPGSQIESTEAEYVVPDVIVRK
HHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCEEEC
DNERWLVELNQESVPRLRVNAQYAGFVRRADTSADNTFMRNQLQEARWFIKSLQSRNETL
CCCCEEEEECCCCCCEEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MKVATQIVEHQRGFLEYGDEAMKPLVLHDIAEAVGMHESTISRVTTQKFMHTPRGIYELK
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHH
YFFSSHVSTSEGGECSSTAIRAIIKKLVAAENQKKPLSDSKIAGLLEAQGIQVARRTVAK
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
YRESLGIAPSSERKRLM
HHHHCCCCCCCHHCCCC
>Mature Secondary Structure
MKPSLVLRMGQQLTMTPQLQQAIRLLQLSTLDLQQEIQEALESNPMLERQEDGDDFDNSD
CCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
PMADNAEQKPNTEIQEPSYQETAPTVDNLEDGEWNERIPNELPVDTAWEDVYQTSASSLP
CCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHCCC
SSDDDEWDFTTRTSAGESLQSHLLWQLNLAPMSDTDRLIAVTLIDCINNQGYLDETLEEI
CCCCCCCCCCCCCCHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHH
LDAFDPELDIELDEIEAVLHRIQQFEPAGIGARNLGECLLLQLRQLSAKTPWLAEAKRLV
HHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
TDYIDLLGSRDYSQLMRRMKLKEDELRQVIELVQSLNPRPGSQIESTEAEYVVPDVIVRK
HHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCEEEC
DNERWLVELNQESVPRLRVNAQYAGFVRRADTSADNTFMRNQLQEARWFIKSLQSRNETL
CCCCEEEEECCCCCCEEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MKVATQIVEHQRGFLEYGDEAMKPLVLHDIAEAVGMHESTISRVTTQKFMHTPRGIYELK
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHH
YFFSSHVSTSEGGECSSTAIRAIIKKLVAAENQKKPLSDSKIAGLLEAQGIQVARRTVAK
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
YRESLGIAPSSERKRLM
HHHHCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2602128; 12534463 [H]