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The map label for this gene is pnp [H]

Identifier: 77457010

GI number: 77457010

Start: 918342

End: 920447

Strand: Direct

Name: pnp [H]

Synonym: Pfl01_0783

Alternate gene names: 77457010

Gene position: 918342-920447 (Clockwise)

Preceding gene: 77457009

Following gene: 77457011

Centisome position: 14.26

GC content: 59.45

Gene sequence:

>2106_bases
GTGAACCCGGTAATCAAAAAATTCCAGTTCGGTCAGTCGACCGTTACCCTCGAGACTGGCCGTATCGCCCGTCAGGCCTC
CGGCGCAGTATTGGTCACCGTTGACGACGACGTCAGCGTATTGGTGACCGTAGTCGGTGCCAAGCAAGCCGATCCGGGCA
AGGGCTTCTTCCCTCTGTCCGTTCACTACCAGGAAAAGACTTACGCTGCCGGTAAGATCCCTGGCGGTTTCTTCAAGCGT
GAAGGCCGTCCTTCCGAGAAAGAAACCCTGACTTCCCGACTGATCGACCGTCCGATCCGTCCACTGTTCCCTGAAGGTTT
CATGAACGAAGTGCAGGTTGTCTGCACCGTCGTTTCCACCAGCAAGAAGACCGATCCGGACATCGCTGCGATGATCGGTA
CCTCGGCTGCCCTGGCGATCTCCGGCATTCCTTTTGACGGCCCGATCGGCGCCGCCCGCGTGGCTTTCCACGAAAGCACC
GGCTACCTGCTGAACCCGACTTACGAGCAGCAAGCTGCCTCGAGCCTGGACATGGTCGTTGCCGGTACTTCGGACGCCGT
GCTGATGGTTGAATCGGAAGCCAAAGAGCTGACCGAAGACCAGATGCTGGGCGCAGTACTGTTTGCTCACGACGAGTTCC
AGGTCGTGATCAACGCCGTTAAAGAACTGGCTGCCGAAGCGGCCAAGCCAACCTGGACCTGGGCGCCTGCTCCAGAAGCC
ACCGAGCTGCTGGCTGCTATCCGTTCCGAGTTCGGCGAAGCGATCTCCCAGGCTTACACCATCACCATCAAGGCCGACCG
TTATGCGCGTCTGGGCGAGCTGCGTGACCAGGTTGTTGCCAAGCTGTCCGGTGAAGAAGGCCAGCCTTCGGCTGCCGACG
TCAAAGCCGCTTTCGGTGAAATCGAATACCGCACCGTTCGCGAAAACATCGTCAACGGCAAGCCACGTATCGACGGTCGC
GACACCCGCACCGTACGTCCGCTGAACATCGAAGTCGGCGTTCTGCCGAAGACTCACGGTTCGGCACTGTTCACCCGTGG
TGAAACCCAGGCTCTGGTCGTCGCGACGCTGGGCACTGCCCGTGACGCACAACTGCTGGACACTCTGGAAGGCGAGAAAA
AAGACCCGTTCATGCTGCACTACAACTTCCCTCCGTTCTCGGTGGGCGAGTGTGGTCGCATGGGTGGCGCCGGTCGTCGC
GAAATCGGTCACGGCCGTCTGGCCCGTCGCTCGGTTTCGGCCATGCTGCCTGCCGCTGACGTGTTCCCGTACACCATCCG
TGTGGTATCGGAAATCACCGAATCCAACGGTTCGAGCTCGATGGCTTCCGTTTGCGGCGCTTCCCTGGCCCTGATGGACG
CGGGTGTTCCGATGAAGGCACCGGTTGCCGGTATCGCCATGGGTCTGGTTAAGGAAGGCGAGAAATTCGCCGTCCTGACC
GACATCCTGGGTGACGAAGACCACTTGGGCGACATGGACTTCAAAGTAGCCGGTACCGCCAAAGGTGTGACCGCGCTGCA
GATGGACATCAAGATCAAGGGCATCACCGAAGAGATCATGGAAATCGCTCTGGGCCAAGCCCTGGAAGCGCGCCTGAACA
TCCTCGGCCAGATGAACCAGATCATCGGCCAGTCGCGTACCGAACTGTCGGCCAACGCTCCGACCATGATCGCGATGAAG
ATCGACACCGACAAGATCCGTGACGTCATCGGTAAAGGCGGCGCGACCATCCGTGCGATCTGCGAAGAGACCAAGGCTTC
GATCGACATCGAAGACGACGGTTCGATCAAGATCTTCGGCGAAACCAAGGAAGCCGCTGAAGCAGCTCGTCAGCGCGTTC
TGGGTATCACCGCAGAGGCCGAGATCGGCAAGATCTACGTCGGCAAGGTTGAACGCATCGTCGACTTCGGCGCATTCGTC
AACATCCTGCCGGGCAAGGACGGTCTGGTGCACATCTCGATGCTGAGCGACGCTCGCGTAGAGAAAGTGACCGACATCCT
GAAAGAAGGCCAGGAAGTGGAAGTGCTGGTACTGGACGTGGACAACCGCGGCCGTATCAAGCTGTCCATCAAAGACGTGG
CAGCTGCCAAGGCTTCGGGCGTTTAA

Upstream 100 bases:

>100_bases
CACGTTTTACCCTGGACAGGCGTCGGGCCGATTCCCGGCATTGCCCAAGAATTTCGCAAGAAACCAGTTCCCCCAAGAGC
CACAAAGAAGGTAGGACACC

Downstream 100 bases:

>100_bases
TCACCCCGCAGCTCTAGCGCAATGAAAGATGCCCCGCCGTGAAAACGGCGGGGCATTTTTTTTGTCCATGAAATATCGAG
TCATTCGGCGAAGTTGCCGA

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 701; Mature: 701

Protein sequence:

>701_residues
MNPVIKKFQFGQSTVTLETGRIARQASGAVLVTVDDDVSVLVTVVGAKQADPGKGFFPLSVHYQEKTYAAGKIPGGFFKR
EGRPSEKETLTSRLIDRPIRPLFPEGFMNEVQVVCTVVSTSKKTDPDIAAMIGTSAALAISGIPFDGPIGAARVAFHEST
GYLLNPTYEQQAASSLDMVVAGTSDAVLMVESEAKELTEDQMLGAVLFAHDEFQVVINAVKELAAEAAKPTWTWAPAPEA
TELLAAIRSEFGEAISQAYTITIKADRYARLGELRDQVVAKLSGEEGQPSAADVKAAFGEIEYRTVRENIVNGKPRIDGR
DTRTVRPLNIEVGVLPKTHGSALFTRGETQALVVATLGTARDAQLLDTLEGEKKDPFMLHYNFPPFSVGECGRMGGAGRR
EIGHGRLARRSVSAMLPAADVFPYTIRVVSEITESNGSSSMASVCGASLALMDAGVPMKAPVAGIAMGLVKEGEKFAVLT
DILGDEDHLGDMDFKVAGTAKGVTALQMDIKIKGITEEIMEIALGQALEARLNILGQMNQIIGQSRTELSANAPTMIAMK
IDTDKIRDVIGKGGATIRAICEETKASIDIEDDGSIKIFGETKEAAEAARQRVLGITAEAEIGKIYVGKVERIVDFGAFV
NILPGKDGLVHISMLSDARVEKVTDILKEGQEVEVLVLDVDNRGRIKLSIKDVAAAKASGV

Sequences:

>Translated_701_residues
MNPVIKKFQFGQSTVTLETGRIARQASGAVLVTVDDDVSVLVTVVGAKQADPGKGFFPLSVHYQEKTYAAGKIPGGFFKR
EGRPSEKETLTSRLIDRPIRPLFPEGFMNEVQVVCTVVSTSKKTDPDIAAMIGTSAALAISGIPFDGPIGAARVAFHEST
GYLLNPTYEQQAASSLDMVVAGTSDAVLMVESEAKELTEDQMLGAVLFAHDEFQVVINAVKELAAEAAKPTWTWAPAPEA
TELLAAIRSEFGEAISQAYTITIKADRYARLGELRDQVVAKLSGEEGQPSAADVKAAFGEIEYRTVRENIVNGKPRIDGR
DTRTVRPLNIEVGVLPKTHGSALFTRGETQALVVATLGTARDAQLLDTLEGEKKDPFMLHYNFPPFSVGECGRMGGAGRR
EIGHGRLARRSVSAMLPAADVFPYTIRVVSEITESNGSSSMASVCGASLALMDAGVPMKAPVAGIAMGLVKEGEKFAVLT
DILGDEDHLGDMDFKVAGTAKGVTALQMDIKIKGITEEIMEIALGQALEARLNILGQMNQIIGQSRTELSANAPTMIAMK
IDTDKIRDVIGKGGATIRAICEETKASIDIEDDGSIKIFGETKEAAEAARQRVLGITAEAEIGKIYVGKVERIVDFGAFV
NILPGKDGLVHISMLSDARVEKVTDILKEGQEVEVLVLDVDNRGRIKLSIKDVAAAKASGV
>Mature_701_residues
MNPVIKKFQFGQSTVTLETGRIARQASGAVLVTVDDDVSVLVTVVGAKQADPGKGFFPLSVHYQEKTYAAGKIPGGFFKR
EGRPSEKETLTSRLIDRPIRPLFPEGFMNEVQVVCTVVSTSKKTDPDIAAMIGTSAALAISGIPFDGPIGAARVAFHEST
GYLLNPTYEQQAASSLDMVVAGTSDAVLMVESEAKELTEDQMLGAVLFAHDEFQVVINAVKELAAEAAKPTWTWAPAPEA
TELLAAIRSEFGEAISQAYTITIKADRYARLGELRDQVVAKLSGEEGQPSAADVKAAFGEIEYRTVRENIVNGKPRIDGR
DTRTVRPLNIEVGVLPKTHGSALFTRGETQALVVATLGTARDAQLLDTLEGEKKDPFMLHYNFPPFSVGECGRMGGAGRR
EIGHGRLARRSVSAMLPAADVFPYTIRVVSEITESNGSSSMASVCGASLALMDAGVPMKAPVAGIAMGLVKEGEKFAVLT
DILGDEDHLGDMDFKVAGTAKGVTALQMDIKIKGITEEIMEIALGQALEARLNILGQMNQIIGQSRTELSANAPTMIAMK
IDTDKIRDVIGKGGATIRAICEETKASIDIEDDGSIKIFGETKEAAEAARQRVLGITAEAEIGKIYVGKVERIVDFGAFV
NILPGKDGLVHISMLSDARVEKVTDILKEGQEVEVLVLDVDNRGRIKLSIKDVAAAKASGV

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=706, Percent_Identity=35.8356940509915, Blast_Score=421, Evalue=1e-117,
Organism=Escherichia coli, GI145693187, Length=693, Percent_Identity=62.6262626262626, Blast_Score=868, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=715, Percent_Identity=33.7062937062937, Blast_Score=330, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI17535281, Length=77, Percent_Identity=48.0519480519481, Blast_Score=69, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320850, Length=103, Percent_Identity=35.9223300970874, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI281362905, Length=710, Percent_Identity=36.056338028169, Blast_Score=421, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24651641, Length=710, Percent_Identity=36.056338028169, Blast_Score=421, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24651643, Length=710, Percent_Identity=36.056338028169, Blast_Score=421, Evalue=1e-118,
Organism=Drosophila melanogaster, GI161079377, Length=657, Percent_Identity=35.7686453576865, Blast_Score=384, Evalue=1e-107,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 74846; Mature: 74846

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPVIKKFQFGQSTVTLETGRIARQASGAVLVTVDDDVSVLVTVVGAKQADPGKGFFPLS
CCCHHHHHCCCCCEEEEECCCHHHHCCCEEEEEECCCCEEEEEEEECCCCCCCCCEEEEE
VHYQEKTYAAGKIPGGFFKREGRPSEKETLTSRLIDRPIRPLFPEGFMNEVQVVCTVVST
EEECCCEEECCCCCCCHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHEEEEEEECC
SKKTDPDIAAMIGTSAALAISGIPFDGPIGAARVAFHESTGYLLNPTYEQQAASSLDMVV
CCCCCCCHHHHHCCCEEEEEECCCCCCCCCCEEEEEECCCCCEECCCHHHHHHCCCEEEE
AGTSDAVLMVESEAKELTEDQMLGAVLFAHDEFQVVINAVKELAAEAAKPTWTWAPAPEA
ECCCCEEEEEECCHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHCCCCEECCCCCHH
TELLAAIRSEFGEAISQAYTITIKADRYARLGELRDQVVAKLSGEEGQPSAADVKAAFGE
HHHHHHHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCC
IEYRTVRENIVNGKPRIDGRDTRTVRPLNIEVGVLPKTHGSALFTRGETQALVVATLGTA
CHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCEEEECCCCCEEEEEECCCC
RDAQLLDTLEGEKKDPFMLHYNFPPFSVGECGRMGGAGRREIGHGRLARRSVSAMLPAAD
CCHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHH
VFPYTIRVVSEITESNGSSSMASVCGASLALMDAGVPMKAPVAGIAMGLVKEGEKFAVLT
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHCCCCEEEEE
DILGDEDHLGDMDFKVAGTAKGVTALQMDIKIKGITEEIMEIALGQALEARLNILGQMNQ
HHCCCCCCCCCCCEEEECCCCCEEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHH
IIGQSRTELSANAPTMIAMKIDTDKIRDVIGKGGATIRAICEETKASIDIEDDGSIKIFG
HHCCCCCCCCCCCCEEEEEEECHHHHHHHHCCCCCHHHHHHHHHCCEEEECCCCCEEEEE
ETKEAAEAARQRVLGITAEAEIGKIYVGKVERIVDFGAFVNILPGKDGLVHISMLSDARV
CCHHHHHHHHHHHEEEEECCCCCEEEEHHHHHHHHHCHHEEEECCCCCEEEEEECCCHHH
EKVTDILKEGQEVEVLVLDVDNRGRIKLSIKDVAAAKASGV
HHHHHHHHCCCCEEEEEEEECCCCEEEEEEHHHHHHHCCCC
>Mature Secondary Structure
MNPVIKKFQFGQSTVTLETGRIARQASGAVLVTVDDDVSVLVTVVGAKQADPGKGFFPLS
CCCHHHHHCCCCCEEEEECCCHHHHCCCEEEEEECCCCEEEEEEEECCCCCCCCCEEEEE
VHYQEKTYAAGKIPGGFFKREGRPSEKETLTSRLIDRPIRPLFPEGFMNEVQVVCTVVST
EEECCCEEECCCCCCCHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHEEEEEEECC
SKKTDPDIAAMIGTSAALAISGIPFDGPIGAARVAFHESTGYLLNPTYEQQAASSLDMVV
CCCCCCCHHHHHCCCEEEEEECCCCCCCCCCEEEEEECCCCCEECCCHHHHHHCCCEEEE
AGTSDAVLMVESEAKELTEDQMLGAVLFAHDEFQVVINAVKELAAEAAKPTWTWAPAPEA
ECCCCEEEEEECCHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHCCCCEECCCCCHH
TELLAAIRSEFGEAISQAYTITIKADRYARLGELRDQVVAKLSGEEGQPSAADVKAAFGE
HHHHHHHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCC
IEYRTVRENIVNGKPRIDGRDTRTVRPLNIEVGVLPKTHGSALFTRGETQALVVATLGTA
CHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEEECCCCCCCEEEECCCCCEEEEEECCCC
RDAQLLDTLEGEKKDPFMLHYNFPPFSVGECGRMGGAGRREIGHGRLARRSVSAMLPAAD
CCHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHH
VFPYTIRVVSEITESNGSSSMASVCGASLALMDAGVPMKAPVAGIAMGLVKEGEKFAVLT
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHCCCCEEEEE
DILGDEDHLGDMDFKVAGTAKGVTALQMDIKIKGITEEIMEIALGQALEARLNILGQMNQ
HHCCCCCCCCCCCEEEECCCCCEEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHH
IIGQSRTELSANAPTMIAMKIDTDKIRDVIGKGGATIRAICEETKASIDIEDDGSIKIFG
HHCCCCCCCCCCCCEEEEEEECHHHHHHHHCCCCCHHHHHHHHHCCEEEECCCCCEEEEE
ETKEAAEAARQRVLGITAEAEIGKIYVGKVERIVDFGAFVNILPGKDGLVHISMLSDARV
CCHHHHHHHHHHHEEEEECCCCCEEEEHHHHHHHHHCHHEEEECCCCCEEEEEECCCHHH
EKVTDILKEGQEVEVLVLDVDNRGRIKLSIKDVAAAKASGV
HHHHHHHHCCCCEEEEEEEECCCCEEEEEEHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA