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The map label for this gene is truB [H]

Identifier: 77457008

GI number: 77457008

Start: 916819

End: 917736

Strand: Direct

Name: truB [H]

Synonym: Pfl01_0781

Alternate gene names: 77457008

Gene position: 916819-917736 (Clockwise)

Preceding gene: 77457007

Following gene: 77457009

Centisome position: 14.24

GC content: 60.78

Gene sequence:

>918_bases
GTGGCTCAGGTCAAACGTATCCGTCGTAACGTCAGCGGCATCATCCTGCTCGACAAGCCGCTGGGGTTCACCTCCAACGC
AGCCTTGCAGAAGGTTCGCTGGTTGCTCAACGCCGAAAAGGCCGGTCACACCGGCAGTCTCGACCCTCTGGCCACCGGCG
TGCTGCCGTTGTGCTTTGGCGAGGCGACCAAGTTCTCGCAGTACCTGCTCGATTCCGACAAGGGTTACGAAACCCTGGCG
CAACTGGGCAAGACCACCACCACGGCAGACGCCGAAGGTGAGGTTTTGCAGGAACGTCCGGTGACCGTTGGTCAGGCCGA
CATTGAAGCGGTTCTGCCGAAATTTCGTGGGCAAATCAGTCAGATACCGCCGATGTACTCGGCTCTCAAGCGTGATGGCC
AGCCCCTGTACAAGTTGGCCCGTGCTGGCGAAGTAGTGGAGCGCGAACCGCGTTCTGTTACTATTGCGCGCTTGGAATTG
CTGGCCTTCGAAGGCGATACTGCGCGTCTTGCGGTGGATTGCAGCAAGGGCACCTATATTCGCACCCTGGTGGAGGATAT
CGGTGAGCAACTCGGTTGTGGTGCGTACGTCGCTGAACTGCGTCGTACCCAGGCCGGGCCTTTCACCCTGGCGCAGACCG
TGACCCTCGAAGAGCTGGAAGCGGTACATGCCGAAGGCGGCAACGAAGCGGTCGACCGCTTCCTGATGCCATCGGACAGC
GGCCTGCAGGATTGGCCGTTGCTGCAGTTCTCGGAAGCGAGCGCGTTCTACTGGCTCAACGGCCAGCCGGTACGTGCCCC
GGATGCTCCGAAGTTCGGCATGGTGCGGGTACAGGATCACAACGGTCGCTTCATCGGTATCGGTGAAGTGAGCGAAGACG
GGCGCATCGCGCCGCGTCGACTGATTCGGTCAGAATGA

Upstream 100 bases:

>100_bases
GTGCGGGGTGCGCACCTGTCGGCCCTGATCGAGCGCGCCGTGGCTGAGGACAATCAGCACGTGGCTGCGGCAGAACCCGA
AGACACCAAGGAGTAACTCG

Downstream 100 bases:

>100_bases
CCGGAAACCGGTCTGTGTAACAGCAGGCCGGCGAGTGTGGCTGTCAACAGGCACGGTCACTACTCTTTTATAGATACAGG
GATTTGTCCCTGGCCTGTTG

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MAQVKRIRRNVSGIILLDKPLGFTSNAALQKVRWLLNAEKAGHTGSLDPLATGVLPLCFGEATKFSQYLLDSDKGYETLA
QLGKTTTTADAEGEVLQERPVTVGQADIEAVLPKFRGQISQIPPMYSALKRDGQPLYKLARAGEVVEREPRSVTIARLEL
LAFEGDTARLAVDCSKGTYIRTLVEDIGEQLGCGAYVAELRRTQAGPFTLAQTVTLEELEAVHAEGGNEAVDRFLMPSDS
GLQDWPLLQFSEASAFYWLNGQPVRAPDAPKFGMVRVQDHNGRFIGIGEVSEDGRIAPRRLIRSE

Sequences:

>Translated_305_residues
MAQVKRIRRNVSGIILLDKPLGFTSNAALQKVRWLLNAEKAGHTGSLDPLATGVLPLCFGEATKFSQYLLDSDKGYETLA
QLGKTTTTADAEGEVLQERPVTVGQADIEAVLPKFRGQISQIPPMYSALKRDGQPLYKLARAGEVVEREPRSVTIARLEL
LAFEGDTARLAVDCSKGTYIRTLVEDIGEQLGCGAYVAELRRTQAGPFTLAQTVTLEELEAVHAEGGNEAVDRFLMPSDS
GLQDWPLLQFSEASAFYWLNGQPVRAPDAPKFGMVRVQDHNGRFIGIGEVSEDGRIAPRRLIRSE
>Mature_304_residues
AQVKRIRRNVSGIILLDKPLGFTSNAALQKVRWLLNAEKAGHTGSLDPLATGVLPLCFGEATKFSQYLLDSDKGYETLAQ
LGKTTTTADAEGEVLQERPVTVGQADIEAVLPKFRGQISQIPPMYSALKRDGQPLYKLARAGEVVEREPRSVTIARLELL
AFEGDTARLAVDCSKGTYIRTLVEDIGEQLGCGAYVAELRRTQAGPFTLAQTVTLEELEAVHAEGGNEAVDRFLMPSDSG
LQDWPLLQFSEASAFYWLNGQPVRAPDAPKFGMVRVQDHNGRFIGIGEVSEDGRIAPRRLIRSE

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=221, Percent_Identity=42.5339366515837, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI215599015, Length=195, Percent_Identity=29.7435897435897, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI4503337, Length=195, Percent_Identity=29.7435897435897, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI2367200, Length=309, Percent_Identity=50.8090614886731, Blast_Score=316, Evalue=1e-87,
Organism=Caenorhabditis elegans, GI17553978, Length=195, Percent_Identity=29.2307692307692, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324037, Length=193, Percent_Identity=35.2331606217617, Blast_Score=103, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6323204, Length=200, Percent_Identity=29, Blast_Score=83, Evalue=6e-17,
Organism=Drosophila melanogaster, GI281364189, Length=197, Percent_Identity=28.9340101522843, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI281364187, Length=197, Percent_Identity=28.9340101522843, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI281364185, Length=197, Percent_Identity=28.9340101522843, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI281364183, Length=197, Percent_Identity=28.9340101522843, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI62471759, Length=197, Percent_Identity=28.9340101522843, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17975520, Length=197, Percent_Identity=28.9340101522843, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240 [H]

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 33383; Mature: 33252

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQVKRIRRNVSGIILLDKPLGFTSNAALQKVRWLLNAEKAGHTGSLDPLATGVLPLCFG
CCHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHC
EATKFSQYLLDSDKGYETLAQLGKTTTTADAEGEVLQERPVTVGQADIEAVLPKFRGQIS
CHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHCHHH
QIPPMYSALKRDGQPLYKLARAGEVVEREPRSVTIARLELLAFEGDTARLAVDCSKGTYI
CCCHHHHHHHHCCHHHHHHHHHCHHHHCCCCEEEEEEEEEEEECCCCEEEEEECCCCCHH
RTLVEDIGEQLGCGAYVAELRRTQAGPFTLAQTVTLEELEAVHAEGGNEAVDRFLMPSDS
HHHHHHHHHHHCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCC
GLQDWPLLQFSEASAFYWLNGQPVRAPDAPKFGMVRVQDHNGRFIGIGEVSEDGRIAPRR
CCCCCCCEEECCCCEEEEECCCEECCCCCCCCCEEEEECCCCCEEEECCCCCCCCCCHHH
LIRSE
HHCCC
>Mature Secondary Structure 
AQVKRIRRNVSGIILLDKPLGFTSNAALQKVRWLLNAEKAGHTGSLDPLATGVLPLCFG
CHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHC
EATKFSQYLLDSDKGYETLAQLGKTTTTADAEGEVLQERPVTVGQADIEAVLPKFRGQIS
CHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHCHHH
QIPPMYSALKRDGQPLYKLARAGEVVEREPRSVTIARLELLAFEGDTARLAVDCSKGTYI
CCCHHHHHHHHCCHHHHHHHHHCHHHHCCCCEEEEEEEEEEEECCCCEEEEEECCCCCHH
RTLVEDIGEQLGCGAYVAELRRTQAGPFTLAQTVTLEELEAVHAEGGNEAVDRFLMPSDS
HHHHHHHHHHHCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCC
GLQDWPLLQFSEASAFYWLNGQPVRAPDAPKFGMVRVQDHNGRFIGIGEVSEDGRIAPRR
CCCCCCCEEECCCCEEEEECCCEECCCCCCCCCEEEEECCCCCEEEECCCCCCCCCCHHH
LIRSE
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA