Click here to switch to the map view.

The map label for this gene is intA [H]

Identifier: 77456974

GI number: 77456974

Start: 877388

End: 878644

Strand: Reverse

Name: intA [H]

Synonym: Pfl01_0747

Alternate gene names: 77456974

Gene position: 878644-877388 (Counterclockwise)

Preceding gene: 77456975

Following gene: 77456970

Centisome position: 13.65

GC content: 56.48

Gene sequence:

>1257_bases
ATGCCTACTAACGCAACCCGCCTCTCCGATCGCCAGCTCAAGGCAGTCAAGGCAACTGGTAAAGACTTCGTCCTCAGCGA
CGGCGACGGCCTGCAGCTTCGAGTACGCGCCAGCGGCTCGATGATGTGGAATTTCAATTACCGCGAGCCGTTGACCAGAA
GCCGTCTTAATATGGCGCTCGGTCCATACCCCGACCTCTCGCTAGCCAACGCCCGGAAGAAAGCAGCAGAGGCGCGTGAG
CTGCTCGCTTTGGGCATTGATCCCAAAACCCAGCGTGATGAGGTAAGGCAAGCCAAACTTGCTGAGACTGAACACACGTT
CGAGAAGGTGGCCACCGCCTGGTTCGAGCTGAAGAAAGACTCCGTAACCAAAGCCTACGCCGAAGACATTTGGAGATCGC
TGACGCTCCATGTGTTCCCAAGCATGAAAACAACGCCGCTTTCTCAAATCACTGCTCCGATGGTTATCAAGATCCTTCGC
CCAATCGAGGCCAAAGGGAGTCTCGAAACCGTGAAGCGATTGAGTCAACGACTCAACGAGATCATGACCTACGGGGTCAA
CTCCGGGCTGATATTTGCGAACCCCCTCAATGGAATTCGGGCGGTCTTCAAGAAACCCAAGAAAGAGAACATGGCTGCGC
TACCACCTGAGGAGCTTCCCGAGCTCATGATGGAAATCGCGAATGCCAGCATCAAGCGCACGACCCGCTGCCTGATCGAA
TGGCAGTTGCACACGATGACCCGCCCCGCCGAAGCAGCCACCACACGATGGGCAGATATCGACTTCGACAAGCGCATTTG
GACCATTCCGCCAGAGCGCATGAAAAAGCGGCGCCCGCACACAATCCCCCTCACCGATCAGGCACTTTCGTTACTCGAAA
CGCTCAAGCAACTCAGTGGTAACAGAGAGTACGTGTTCCCCTCAGATAGAAACCCCCGCACCCATGCCAATAGCCAGACC
GCCAACATGGCGTTGAAGCGCATGGGCTTTCAGGACCGTCTAGTCAGCCACGGCTTGCGCTCGATGGCGAGCACCATCCT
GAATGAGCATGGCTGGGATCCGGAGCTGATTGAGGTCGCACTTGCGCATGTCGACAAGGACGAAGTTCGTAGCGCCTACA
ACCGGACGGATTACATCGAACGCCGGCGTCCGATGATGGCTTGGTGGAGTGAACATATCCAGAAGGCGGCCACCGGCAGC
CTGTCCGCATCGGCCATCAATCAAACTAGAGATCGCAACGTCGTACCGATTCGCTGA

Upstream 100 bases:

>100_bases
GGAGGATTTCTGAGTTCCAAGCGCTTTGGTATCCCAGGTGGTATCCCGAGTTACCAAATGCTATTTTCAGGATACCAAAA
CGGTGCTGGAGGTAGCCCCC

Downstream 100 bases:

>100_bases
ACATCCAAAAGGTACGCGGCACCGGCTACGATTCGTGTCGTGTAGTAGGTGAAAGGAGTGACGACAGTTGCCAGATCTCT
GGTATGCCTGCGCTTTTTCA

Product: Phage integrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MPTNATRLSDRQLKAVKATGKDFVLSDGDGLQLRVRASGSMMWNFNYREPLTRSRLNMALGPYPDLSLANARKKAAEARE
LLALGIDPKTQRDEVRQAKLAETEHTFEKVATAWFELKKDSVTKAYAEDIWRSLTLHVFPSMKTTPLSQITAPMVIKILR
PIEAKGSLETVKRLSQRLNEIMTYGVNSGLIFANPLNGIRAVFKKPKKENMAALPPEELPELMMEIANASIKRTTRCLIE
WQLHTMTRPAEAATTRWADIDFDKRIWTIPPERMKKRRPHTIPLTDQALSLLETLKQLSGNREYVFPSDRNPRTHANSQT
ANMALKRMGFQDRLVSHGLRSMASTILNEHGWDPELIEVALAHVDKDEVRSAYNRTDYIERRRPMMAWWSEHIQKAATGS
LSASAINQTRDRNVVPIR

Sequences:

>Translated_418_residues
MPTNATRLSDRQLKAVKATGKDFVLSDGDGLQLRVRASGSMMWNFNYREPLTRSRLNMALGPYPDLSLANARKKAAEARE
LLALGIDPKTQRDEVRQAKLAETEHTFEKVATAWFELKKDSVTKAYAEDIWRSLTLHVFPSMKTTPLSQITAPMVIKILR
PIEAKGSLETVKRLSQRLNEIMTYGVNSGLIFANPLNGIRAVFKKPKKENMAALPPEELPELMMEIANASIKRTTRCLIE
WQLHTMTRPAEAATTRWADIDFDKRIWTIPPERMKKRRPHTIPLTDQALSLLETLKQLSGNREYVFPSDRNPRTHANSQT
ANMALKRMGFQDRLVSHGLRSMASTILNEHGWDPELIEVALAHVDKDEVRSAYNRTDYIERRRPMMAWWSEHIQKAATGS
LSASAINQTRDRNVVPIR
>Mature_417_residues
PTNATRLSDRQLKAVKATGKDFVLSDGDGLQLRVRASGSMMWNFNYREPLTRSRLNMALGPYPDLSLANARKKAAEAREL
LALGIDPKTQRDEVRQAKLAETEHTFEKVATAWFELKKDSVTKAYAEDIWRSLTLHVFPSMKTTPLSQITAPMVIKILRP
IEAKGSLETVKRLSQRLNEIMTYGVNSGLIFANPLNGIRAVFKKPKKENMAALPPEELPELMMEIANASIKRTTRCLIEW
QLHTMTRPAEAATTRWADIDFDKRIWTIPPERMKKRRPHTIPLTDQALSLLETLKQLSGNREYVFPSDRNPRTHANSQTA
NMALKRMGFQDRLVSHGLRSMASTILNEHGWDPELIEVALAHVDKDEVRSAYNRTDYIERRRPMMAWWSEHIQKAATGSL
SASAINQTRDRNVVPIR

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: COG0582

COG function: function code L; Integrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788974, Length=404, Percent_Identity=50.7425742574257, Blast_Score=421, Evalue=1e-119,
Organism=Escherichia coli, GI1788690, Length=385, Percent_Identity=29.0909090909091, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI145693166, Length=401, Percent_Identity=31.6708229426434, Blast_Score=181, Evalue=6e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 47619; Mature: 47488

Theoretical pI: Translated: 10.53; Mature: 10.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTNATRLSDRQLKAVKATGKDFVLSDGDGLQLRVRASGSMMWNFNYREPLTRSRLNMAL
CCCCCCCCCHHHHHHHHHCCCCEEEECCCCCEEEEEECCEEEEECCCCCCHHHHHHHHCC
GPYPDLSLANARKKAAEARELLALGIDPKTQRDEVRQAKLAETEHTFEKVATAWFELKKD
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVTKAYAEDIWRSLTLHVFPSMKTTPLSQITAPMVIKILRPIEAKGSLETVKRLSQRLNE
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
IMTYGVNSGLIFANPLNGIRAVFKKPKKENMAALPPEELPELMMEIANASIKRTTRCLIE
HHHHCCCCCEEEECCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHE
WQLHTMTRPAEAATTRWADIDFDKRIWTIPPERMKKRRPHTIPLTDQALSLLETLKQLSG
EHHHHCCCCHHHHHCCCCCCCCCCEEEECCHHHHHHCCCCCCCCHHHHHHHHHHHHHHCC
NREYVFPSDRNPRTHANSQTANMALKRMGFQDRLVSHGLRSMASTILNEHGWDPELIEVA
CCCEECCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LAHVDKDEVRSAYNRTDYIERRRPMMAWWSEHIQKAATGSLSASAINQTRDRNVVPIR
HHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
PTNATRLSDRQLKAVKATGKDFVLSDGDGLQLRVRASGSMMWNFNYREPLTRSRLNMAL
CCCCCCCCHHHHHHHHHCCCCEEEECCCCCEEEEEECCEEEEECCCCCCHHHHHHHHCC
GPYPDLSLANARKKAAEARELLALGIDPKTQRDEVRQAKLAETEHTFEKVATAWFELKKD
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SVTKAYAEDIWRSLTLHVFPSMKTTPLSQITAPMVIKILRPIEAKGSLETVKRLSQRLNE
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
IMTYGVNSGLIFANPLNGIRAVFKKPKKENMAALPPEELPELMMEIANASIKRTTRCLIE
HHHHCCCCCEEEECCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHE
WQLHTMTRPAEAATTRWADIDFDKRIWTIPPERMKKRRPHTIPLTDQALSLLETLKQLSG
EHHHHCCCCHHHHHCCCCCCCCCCEEEECCHHHHHHCCCCCCCCHHHHHHHHHHHHHHCC
NREYVFPSDRNPRTHANSQTANMALKRMGFQDRLVSHGLRSMASTILNEHGWDPELIEVA
CCCEECCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LAHVDKDEVRSAYNRTDYIERRRPMMAWWSEHIQKAATGSLSASAINQTRDRNVVPIR
HHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7511583; 9278503; 9205837; 7524073 [H]