Click here to switch to the map view.

The map label for this gene is camA [H]

Identifier: 77454851

GI number: 77454851

Start: 260151

End: 261359

Strand: Direct

Name: camA [H]

Synonym: pREL1_0284

Alternate gene names: 77454851

Gene position: 260151-261359 (Clockwise)

Preceding gene: 77454850

Following gene: 77454852

Centisome position: 95.79

GC content: 68.07

Gene sequence:

>1209_bases
ATGACGCAGGGGACCGCGCTCGTGGTCGGAGCCAGTCACTCGGGGGCTCAACTGGCGGCCAGCCTCCGGCAGGAAGGCTG
GGCCGGCGAGATCATCCTCATCGGTGACGAGGGCGCACTGCCCTACCAGCGACCGCCGCTGTCCAAGGCCTACCTCGCCG
GCAAGAGCACGCTGGAGGAGTTGGCGATCCGGAAGGCGGACTTCTACGCCAAGCAGCAGATCCAGCTGGTCAATGCGCGC
GTGGAGTCGATCGATCGCACAGAGCGGCGGGTGATGCTGGCCGGCGGCAAGAGCATGTCCTACGACAAGCTGGCGCTGTG
CACCGGTGGCCGGGCACGGCGACTCCCCGTGCCGGGAGCCGACCTCCCGGGCGTCCACTACTTGCGCACGTTCTCCGACG
TGGAGCAGATCCGAGAGTCGGCGCAACCGGGTCGGCGAGCGGTCATCGTCGGCGGCGGCTACATCGGACTGGAGACAGCC
GCCTCCCTGCGTGCGCTTGGACTGGACGTCACCGTTCTTGAGGCCGCCGACCGTGTGCTCGAGCGGGTCACCGCCCCGGA
GGTATCGGCGTTCTACGAGCGCGTCCACCGCGACGCGGGAGTGACCGTCCGCACCCGGGCGCTCGTCGAGGCTTTGGGTG
GCGACGACCGGGTCCGAGAGGTCCGGCTGGCCGGTGGTGAAAAAGTCCCAGCGGACCTCGTCGTCGTCGGTGTCGGGCTC
CAGCCCAACACCGAACTCGCTGCCGCCGCGGGTCTCCTCGTCGACGACGGCGTCGTGATCGACGACCGGGCCCGTACCAG
CGATCACGACATCGTGGCCGCGGGCGACTGCACCAGCCACGTGATGGCGAGGTACGGGCGGTGCGTCCGACTTGAGTCCG
TGCCGAGCGCGGTGGAGCAGGCGAAGGTCGCCGCGGCGACCATGTGCGGCAAGGACAAGACCATCGCGGCGCTGCCGTGG
TTCTGGTCGGATCAGTACGATCTCAAGCTCCAGATCGCGGGACTGAACACCGGCTACGACGAGGTCTTGCTCAGTGGTGA
CCCGAGCTGTGATCGCGATCGCGACTTCACCTGCTTCTACTTCCGCGCAGGGGAACTCATTGCCGCTGACTGCGTCAACC
GGCCGCGCGACTTCGCCTTCAGCAAGCGGACGCTCTCTCCGGGACTCCCCGTCGGCAGAGAAGCGTTACTGGGTATCGCA
CCGAGCTGA

Upstream 100 bases:

>100_bases
TCGAGGACATCGAGGTCGTCGGTGAGCCGGAGTACGTGCAGTCTAACTTCGTCAGGGGCATTAGCAAGCTCATGGTGCGC
CTGACCCCGAAGCAGGACGC

Downstream 100 bases:

>100_bases
GCGTCAGCTGGACCACGGGGCCGTCCGACCCGATCGGCGAGCCGTCGACACCGGCTCGCTACCCACCACCGACGGGACAC
CATGCAGAGCACGATGACGA

Product: ferredoxin reductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MTQGTALVVGASHSGAQLAASLRQEGWAGEIILIGDEGALPYQRPPLSKAYLAGKSTLEELAIRKADFYAKQQIQLVNAR
VESIDRTERRVMLAGGKSMSYDKLALCTGGRARRLPVPGADLPGVHYLRTFSDVEQIRESAQPGRRAVIVGGGYIGLETA
ASLRALGLDVTVLEAADRVLERVTAPEVSAFYERVHRDAGVTVRTRALVEALGGDDRVREVRLAGGEKVPADLVVVGVGL
QPNTELAAAAGLLVDDGVVIDDRARTSDHDIVAAGDCTSHVMARYGRCVRLESVPSAVEQAKVAAATMCGKDKTIAALPW
FWSDQYDLKLQIAGLNTGYDEVLLSGDPSCDRDRDFTCFYFRAGELIAADCVNRPRDFAFSKRTLSPGLPVGREALLGIA
PS

Sequences:

>Translated_402_residues
MTQGTALVVGASHSGAQLAASLRQEGWAGEIILIGDEGALPYQRPPLSKAYLAGKSTLEELAIRKADFYAKQQIQLVNAR
VESIDRTERRVMLAGGKSMSYDKLALCTGGRARRLPVPGADLPGVHYLRTFSDVEQIRESAQPGRRAVIVGGGYIGLETA
ASLRALGLDVTVLEAADRVLERVTAPEVSAFYERVHRDAGVTVRTRALVEALGGDDRVREVRLAGGEKVPADLVVVGVGL
QPNTELAAAAGLLVDDGVVIDDRARTSDHDIVAAGDCTSHVMARYGRCVRLESVPSAVEQAKVAAATMCGKDKTIAALPW
FWSDQYDLKLQIAGLNTGYDEVLLSGDPSCDRDRDFTCFYFRAGELIAADCVNRPRDFAFSKRTLSPGLPVGREALLGIA
PS
>Mature_401_residues
TQGTALVVGASHSGAQLAASLRQEGWAGEIILIGDEGALPYQRPPLSKAYLAGKSTLEELAIRKADFYAKQQIQLVNARV
ESIDRTERRVMLAGGKSMSYDKLALCTGGRARRLPVPGADLPGVHYLRTFSDVEQIRESAQPGRRAVIVGGGYIGLETAA
SLRALGLDVTVLEAADRVLERVTAPEVSAFYERVHRDAGVTVRTRALVEALGGDDRVREVRLAGGEKVPADLVVVGVGLQ
PNTELAAAAGLLVDDGVVIDDRARTSDHDIVAAGDCTSHVMARYGRCVRLESVPSAVEQAKVAAATMCGKDKTIAALPWF
WSDQYDLKLQIAGLNTGYDEVLLSGDPSCDRDRDFTCFYFRAGELIAADCVNRPRDFAFSKRTLSPGLPVGREALLGIAP
S

Specific function: The oxidation of camphor by cytochrome P450-CAM requires the participation of a flavoprotein, putidaredoxin reductase, and an iron-sulfur protein, putidaredoxin, to mediate the transfer of electrons from NADH to P450 for oxygen activation [H]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI65787454, Length=374, Percent_Identity=29.6791443850267, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI21389617, Length=374, Percent_Identity=29.6791443850267, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI226437568, Length=374, Percent_Identity=29.6791443850267, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI22202629, Length=342, Percent_Identity=27.1929824561404, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI4757732, Length=342, Percent_Identity=27.1929824561404, Blast_Score=99, Evalue=5e-21,
Organism=Escherichia coli, GI1788892, Length=389, Percent_Identity=35.73264781491, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1789065, Length=279, Percent_Identity=30.4659498207885, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1789765, Length=314, Percent_Identity=29.6178343949045, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17559934, Length=370, Percent_Identity=27.8378378378378, Blast_Score=142, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI32564386, Length=312, Percent_Identity=25.9615384615385, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24639257, Length=373, Percent_Identity=29.2225201072386, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI281359715, Length=373, Percent_Identity=29.2225201072386, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI281359713, Length=373, Percent_Identity=29.2225201072386, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24639250, Length=373, Percent_Identity=29.2225201072386, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI18543267, Length=373, Percent_Identity=29.2225201072386, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24639252, Length=373, Percent_Identity=29.2225201072386, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24585130, Length=365, Percent_Identity=23.5616438356164, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24581020, Length=356, Percent_Identity=25.8426966292135, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI28573993, Length=356, Percent_Identity=25.8426966292135, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 43047; Mature: 42916

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQGTALVVGASHSGAQLAASLRQEGWAGEIILIGDEGALPYQRPPLSKAYLAGKSTLEE
CCCCCEEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHHHCHHHHHH
LAIRKADFYAKQQIQLVNARVESIDRTERRVMLAGGKSMSYDKLALCTGGRARRLPVPGA
HHHHHHHHHHHHHHHHHHHHHHHHCHHHCEEEEECCCCCCCCEEEEECCCCCEECCCCCC
DLPGVHYLRTFSDVEQIRESAQPGRRAVIVGGGYIGLETAASLRALGLDVTVLEAADRVL
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCEECHHHHHHHHHHCCCCHHHHHHHHHH
ERVTAPEVSAFYERVHRDAGVTVRTRALVEALGGDDRVREVRLAGGEKVPADLVVVGVGL
HHHCCCHHHHHHHHHHHCCCCEEHHHHHHHHHCCCHHHEEEEECCCCCCCCEEEEEEECC
QPNTELAAAAGLLVDDGVVIDDRARTSDHDIVAAGDCTSHVMARYGRCVRLESVPSAVEQ
CCCCCHHHHCCEEEECCEEEECCCCCCCCCEEEECCHHHHHHHHCCCEEEEHHHHHHHHH
AKVAAATMCGKDKTIAALPWFWSDQYDLKLQIAGLNTGYDEVLLSGDPSCDRDRDFTCFY
HHHHHHHHCCCCCEEEEECCEECCCEEEEEEEEECCCCHHHEEECCCCCCCCCCCCEEEE
FRAGELIAADCVNRPRDFAFSKRTLSPGLPVGREALLGIAPS
EECCCEEEHHHCCCCHHHCCCCCCCCCCCCCCCHHHEECCCC
>Mature Secondary Structure 
TQGTALVVGASHSGAQLAASLRQEGWAGEIILIGDEGALPYQRPPLSKAYLAGKSTLEE
CCCCEEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHHHCHHHHHH
LAIRKADFYAKQQIQLVNARVESIDRTERRVMLAGGKSMSYDKLALCTGGRARRLPVPGA
HHHHHHHHHHHHHHHHHHHHHHHHCHHHCEEEEECCCCCCCCEEEEECCCCCEECCCCCC
DLPGVHYLRTFSDVEQIRESAQPGRRAVIVGGGYIGLETAASLRALGLDVTVLEAADRVL
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCEECHHHHHHHHHHCCCCHHHHHHHHHH
ERVTAPEVSAFYERVHRDAGVTVRTRALVEALGGDDRVREVRLAGGEKVPADLVVVGVGL
HHHCCCHHHHHHHHHHHCCCCEEHHHHHHHHHCCCHHHEEEEECCCCCCCCEEEEEEECC
QPNTELAAAAGLLVDDGVVIDDRARTSDHDIVAAGDCTSHVMARYGRCVRLESVPSAVEQ
CCCCCHHHHCCEEEECCEEEECCCCCCCCCEEEECCHHHHHHHHCCCEEEEHHHHHHHHH
AKVAAATMCGKDKTIAALPWFWSDQYDLKLQIAGLNTGYDEVLLSGDPSCDRDRDFTCFY
HHHHHHHHCCCCCEEEEECCEECCCEEEEEEEEECCCCHHHEEECCCCCCCCCCCCEEEE
FRAGELIAADCVNRPRDFAFSKRTLSPGLPVGREALLGIAPS
EECCCEEEHHHCCCCHHHCCCCCCCCCCCCCCCHHHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2180940; 2613690; 3003058; 12011076; 11524002 [H]