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The map label for this gene is camA [H]

Identifier: 77454828

GI number: 77454828

Start: 236051

End: 237253

Strand: Direct

Name: camA [H]

Synonym: pREL1_0261

Alternate gene names: 77454828

Gene position: 236051-237253 (Clockwise)

Preceding gene: 77454827

Following gene: 77454829

Centisome position: 86.92

GC content: 64.42

Gene sequence:

>1203_bases
ATGACCGCACAGCGCGCCGTCATCATCGGGGCCAGCCATGCTGGGGCTCAACTCGCAGCCAGCCTGCGCCAGGAACGTTG
GACCGGAGAGATCGTCCTCATCGGCGATGAATCAGCGCTGCCGTACCAACGCCCTCCTTTGTCGAAGGCTTACCTGGCCT
CGAAGAGCACGCTCAAGGACCTGGAGATCCGAAGCGTCGAGTTCTACAGCAAGCACGGGATCCAACTCCTGGACGCGACT
GTAGAGGCGATCGACCGCTCAGCAGGAAGCCTTTCGCTCAGCACTGGCGACGCGTTGACCTATGACAAGCTCGCCTTGTG
CACCGGTGCCCGCCCGCGGCGGCTCCCTACCCCGGGGGCCGACCTGGCCGGCGTCTACTACCTACGCACCGCCGCGGATG
TTGGGTTGATTCGAAAGGCCGCGACCCCCGGACGTCGAGCCGTGATCGTCGGCGGGGGCTACATCGGCCTAGAGACGGCC
GCCTCCTTGCGTGCACTGGGTCTGGAGGTCACCGTGTTGGAGGCGACCGAACGCGTCCTCGAGCGGGTCACCGCTCCCGA
AGTCTCCGCATTTTTCGACCGAATCCACCGCAGTGAAGGGGTCGATATCCGCACGGGAGCCCTGGTCGAAGCGCTCACCG
GCGATAGCCGCGTCCGCGAAGTGGTCCTGGCCAGCGGCGAATCAATCCCTGCCGACCTCCTCATTGTCGGAATCGGCGTG
GAGCCCAATGTCGACCTCGCCGCCGCCGCGGACCTTGCTGTTGACAATGGCGTCGTCATCGACGACCACGCCCGGACCAG
TGATCACAACATCGTGGCCGCCGGGGATTGCGCCAGCCACGACATGGCCCGCTACGGACGTCGCATTCGCCTGGAGTCCG
TGCCGAGCGCCGCCGAGCAGGCCAAGGTCGCTGCCGCGACCATTTGCGGAAAGCCTAAGGAGATAAAGGCGCTTCCCTGG
TTCTGGTCGGATCAATACGATCTCAAGCTCCAGATCGCCGGGCTCAACACTGGGTACGACGAAGTCGTCCTCAGCGGAAA
CCCGACCCGTGACCGCGACTTCACCTGCTACTACCTACGCAGTGCAGAGCTGATTGCGGCGGACTGCATCAATCGTCCCC
GCGACTTCATGTTCAGCAAGCGGGTCATCTCGCAGCAGCTCCCCATCGACCGCGCCGAACTGTCACTCGCCGGTGCAGTG
TGA

Upstream 100 bases:

>100_bases
TCGAGAACATCGATGTCATCGGCGAACCCGAATACGTCCAGTCCAACTTCGTGAGAGGGATCAGCAAGATGACGGTCCGC
CTCACCCCGAAATCCGACGC

Downstream 100 bases:

>100_bases
GGCGACTCTGCCTTGAGCTCGATCGATATGACGTCGGCGAGCGATACGCAATTCGAAGGACGAACAGGTCCAAACCTCAT
TCGGCGCGCCCACTAACGAA

Product: ferredoxin reductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MTAQRAVIIGASHAGAQLAASLRQERWTGEIVLIGDESALPYQRPPLSKAYLASKSTLKDLEIRSVEFYSKHGIQLLDAT
VEAIDRSAGSLSLSTGDALTYDKLALCTGARPRRLPTPGADLAGVYYLRTAADVGLIRKAATPGRRAVIVGGGYIGLETA
ASLRALGLEVTVLEATERVLERVTAPEVSAFFDRIHRSEGVDIRTGALVEALTGDSRVREVVLASGESIPADLLIVGIGV
EPNVDLAAAADLAVDNGVVIDDHARTSDHNIVAAGDCASHDMARYGRRIRLESVPSAAEQAKVAAATICGKPKEIKALPW
FWSDQYDLKLQIAGLNTGYDEVVLSGNPTRDRDFTCYYLRSAELIAADCINRPRDFMFSKRVISQQLPIDRAELSLAGAV

Sequences:

>Translated_400_residues
MTAQRAVIIGASHAGAQLAASLRQERWTGEIVLIGDESALPYQRPPLSKAYLASKSTLKDLEIRSVEFYSKHGIQLLDAT
VEAIDRSAGSLSLSTGDALTYDKLALCTGARPRRLPTPGADLAGVYYLRTAADVGLIRKAATPGRRAVIVGGGYIGLETA
ASLRALGLEVTVLEATERVLERVTAPEVSAFFDRIHRSEGVDIRTGALVEALTGDSRVREVVLASGESIPADLLIVGIGV
EPNVDLAAAADLAVDNGVVIDDHARTSDHNIVAAGDCASHDMARYGRRIRLESVPSAAEQAKVAAATICGKPKEIKALPW
FWSDQYDLKLQIAGLNTGYDEVVLSGNPTRDRDFTCYYLRSAELIAADCINRPRDFMFSKRVISQQLPIDRAELSLAGAV
>Mature_399_residues
TAQRAVIIGASHAGAQLAASLRQERWTGEIVLIGDESALPYQRPPLSKAYLASKSTLKDLEIRSVEFYSKHGIQLLDATV
EAIDRSAGSLSLSTGDALTYDKLALCTGARPRRLPTPGADLAGVYYLRTAADVGLIRKAATPGRRAVIVGGGYIGLETAA
SLRALGLEVTVLEATERVLERVTAPEVSAFFDRIHRSEGVDIRTGALVEALTGDSRVREVVLASGESIPADLLIVGIGVE
PNVDLAAAADLAVDNGVVIDDHARTSDHNIVAAGDCASHDMARYGRRIRLESVPSAAEQAKVAAATICGKPKEIKALPWF
WSDQYDLKLQIAGLNTGYDEVVLSGNPTRDRDFTCYYLRSAELIAADCINRPRDFMFSKRVISQQLPIDRAELSLAGAV

Specific function: The oxidation of camphor by cytochrome P450-CAM requires the participation of a flavoprotein, putidaredoxin reductase, and an iron-sulfur protein, putidaredoxin, to mediate the transfer of electrons from NADH to P450 for oxygen activation [H]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI65787454, Length=375, Percent_Identity=29.0666666666667, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI21389617, Length=375, Percent_Identity=29.0666666666667, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI226437568, Length=375, Percent_Identity=29.0666666666667, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI4757732, Length=351, Percent_Identity=27.0655270655271, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI22202629, Length=351, Percent_Identity=27.0655270655271, Blast_Score=96, Evalue=5e-20,
Organism=Escherichia coli, GI1788892, Length=397, Percent_Identity=36.272040302267, Blast_Score=185, Evalue=4e-48,
Organism=Escherichia coli, GI1789065, Length=275, Percent_Identity=30.5454545454545, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI1789765, Length=345, Percent_Identity=28.4057971014493, Blast_Score=92, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17559934, Length=368, Percent_Identity=30.1630434782609, Blast_Score=148, Evalue=5e-36,
Organism=Drosophila melanogaster, GI24639257, Length=398, Percent_Identity=29.1457286432161, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI281359715, Length=398, Percent_Identity=29.1457286432161, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI281359713, Length=398, Percent_Identity=29.1457286432161, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24639250, Length=398, Percent_Identity=29.1457286432161, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI18543267, Length=398, Percent_Identity=29.1457286432161, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24639252, Length=398, Percent_Identity=29.1457286432161, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24585130, Length=396, Percent_Identity=24.7474747474747, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24581020, Length=356, Percent_Identity=25.561797752809, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI28573993, Length=356, Percent_Identity=25.561797752809, Blast_Score=99, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 42851; Mature: 42720

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQRAVIIGASHAGAQLAASLRQERWTGEIVLIGDESALPYQRPPLSKAYLASKSTLKD
CCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHCCCCHHH
LEIRSVEFYSKHGIQLLDATVEAIDRSAGSLSLSTGDALTYDKLALCTGARPRRLPTPGA
CEEEEEEEHHHCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCHHHEECCCCCCCCCCCCC
DLAGVYYLRTAADVGLIRKAATPGRRAVIVGGGYIGLETAASLRALGLEVTVLEATERVL
CCCCEEEEHHHHHHHHHHHCCCCCCEEEEECCCEECHHHHHHHHHHCEEEEEHHHHHHHH
ERVTAPEVSAFFDRIHRSEGVDIRTGALVEALTGDSRVREVVLASGESIPADLLIVGIGV
HHHCCCHHHHHHHHHHHCCCCEEECCHHHHHHCCCHHHHHHHHHCCCCCCCEEEEEEECC
EPNVDLAAAADLAVDNGVVIDDHARTSDHNIVAAGDCASHDMARYGRRIRLESVPSAAEQ
CCCCCHHHHHHEEECCCEEEECCCCCCCCCEEEECCCCHHHHHHCCCEEEECCCCCHHHH
AKVAAATICGKPKEIKALPWFWSDQYDLKLQIAGLNTGYDEVVLSGNPTRDRDFTCYYLR
HHHHHHHHCCCCCCCEEECCEECCCEEEEEEEEECCCCCCEEEECCCCCCCCCEEEEEEE
SAELIAADCINRPRDFMFSKRVISQQLPIDRAELSLAGAV
CCHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHEEEECCC
>Mature Secondary Structure 
TAQRAVIIGASHAGAQLAASLRQERWTGEIVLIGDESALPYQRPPLSKAYLASKSTLKD
CCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHCCCCHHH
LEIRSVEFYSKHGIQLLDATVEAIDRSAGSLSLSTGDALTYDKLALCTGARPRRLPTPGA
CEEEEEEEHHHCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCHHHEECCCCCCCCCCCCC
DLAGVYYLRTAADVGLIRKAATPGRRAVIVGGGYIGLETAASLRALGLEVTVLEATERVL
CCCCEEEEHHHHHHHHHHHCCCCCCEEEEECCCEECHHHHHHHHHHCEEEEEHHHHHHHH
ERVTAPEVSAFFDRIHRSEGVDIRTGALVEALTGDSRVREVVLASGESIPADLLIVGIGV
HHHCCCHHHHHHHHHHHCCCCEEECCHHHHHHCCCHHHHHHHHHCCCCCCCEEEEEEECC
EPNVDLAAAADLAVDNGVVIDDHARTSDHNIVAAGDCASHDMARYGRRIRLESVPSAAEQ
CCCCCHHHHHHEEECCCEEEECCCCCCCCCEEEECCCCHHHHHHCCCEEEECCCCCHHHH
AKVAAATICGKPKEIKALPWFWSDQYDLKLQIAGLNTGYDEVVLSGNPTRDRDFTCYYLR
HHHHHHHHCCCCCCCEEECCEECCCEEEEEEEEECCCCCCEEEECCCCCCCCCEEEEEEE
SAELIAADCINRPRDFMFSKRVISQQLPIDRAELSLAGAV
CCHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2180940; 2613690; 3003058; 12011076; 11524002 [H]