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The map label for this gene is czcO [H]

Identifier: 77454730

GI number: 77454730

Start: 157336

End: 158418

Strand: Direct

Name: czcO [H]

Synonym: pREL1_0163

Alternate gene names: 77454730

Gene position: 157336-158418 (Clockwise)

Preceding gene: 77454729

Following gene: 77454731

Centisome position: 57.93

GC content: 66.85

Gene sequence:

>1083_bases
GTGCACTCGCAAGGGACACCGATCACGGTCGACGTTGTCGTGATCGGTGGTGGTCAAGCCGGAATGGCCGCCGGGTACTA
CCTGCGCCGCTCCGGCCGCGACTTCGTCATCCTCGACGCCGAAACAGCGCCCGGTGGATCCTGGCAGCACTACTGGGACT
CACTGCACCTGTTCTCACCAGCCGAATACTCCTCGCTGCCCGGCTGGCCGATGCCGGGCTGGAGTCCCGGTTTCCCGCCC
GCCTCACACGTCGTGGATTACCTCACCGCCTACGAGACCCGATACGACCTACCGGTCCGCCGCCCGGTCCGCGTCACGTC
CGTGACCCGCTCCGGTGACGGGTATCTCGTCGAATCCGATCACGGCAGCTGGTCCGCGCGGGCCGTCGTCAACACCACCG
GGACGTGGCGGCAACCATTCTGGCCGATCTACCCCGGTATCCGCGAGTTCACCGGCACCCAAATCCATACCACCAGCTAC
CGTTCGCCGACCCCGTTCGCCGGTAGCAAGGTCGCCGTGGTCGGCGGCGGAAACTCTGCCGCGCAGATCGTCGCCGAAGT
CTCCACCGTCGCCGAGACACTCTGGCTGACGAGCCGGGAACCGCGGTTCCTGCCCGACGACGTCGACGGCCGGATCCTTT
TCGACGTCGCCCGTGAACATGCCCGTGAAATCGCTTCAGGTGAAACCGATTCCGGTGGCATCGCCGGCCTCGGCGACATC
GTGATGGTGCCCCCGGTCCAAGCCGCCCGCGAGCGCGGCGTGATGGTGGCGCACCCGATGTTCGACCGCCTCACCGCCAC
CGGATTGGGATTCGGCGATCACGTCGAACAGGTGGACGCCATTATCTGGTGCACCGGATTCCGCCCCGCGCTACGGCACC
TTCAGCCCCTTGGACTCCGAGACGGCGACACCGGACGCATCGAACTCGACGGAGCGCACGTCGCCGGTGAACCCGGATTG
ATTTTCCTCGGATACGGCGACTGGACCGGTCCCGCATCGGCCACCCTGATCGGAGTCGGCCGCACCGCCCGCGACGCCAT
CACCGCACTCGACGACGACCTCTTCACAGTCCCGGCCATCTGA

Upstream 100 bases:

>100_bases
ACATCGCTGCCCGCGTCGACGCCCTGGCCGGGCGTCTGACCACGTAATCGACGTATCACCCCGGCTGTCAGCAGAAGATC
TGGATAAGGAAAGGACACTC

Downstream 100 bases:

>100_bases
GCAACATCCGAAACAAAACAGAGGACACATCATGAGCACCATCGAAGTATTCGAACCCGCCCTGTGCTGCAACACCGGCG
TCTGCGGCGACGACGTCGAC

Product: putative FAD-dependent oxidoreductase

Products: NADP+; reduced thioredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MHSQGTPITVDVVVIGGGQAGMAAGYYLRRSGRDFVILDAETAPGGSWQHYWDSLHLFSPAEYSSLPGWPMPGWSPGFPP
ASHVVDYLTAYETRYDLPVRRPVRVTSVTRSGDGYLVESDHGSWSARAVVNTTGTWRQPFWPIYPGIREFTGTQIHTTSY
RSPTPFAGSKVAVVGGGNSAAQIVAEVSTVAETLWLTSREPRFLPDDVDGRILFDVAREHAREIASGETDSGGIAGLGDI
VMVPPVQAARERGVMVAHPMFDRLTATGLGFGDHVEQVDAIIWCTGFRPALRHLQPLGLRDGDTGRIELDGAHVAGEPGL
IFLGYGDWTGPASATLIGVGRTARDAITALDDDLFTVPAI

Sequences:

>Translated_360_residues
MHSQGTPITVDVVVIGGGQAGMAAGYYLRRSGRDFVILDAETAPGGSWQHYWDSLHLFSPAEYSSLPGWPMPGWSPGFPP
ASHVVDYLTAYETRYDLPVRRPVRVTSVTRSGDGYLVESDHGSWSARAVVNTTGTWRQPFWPIYPGIREFTGTQIHTTSY
RSPTPFAGSKVAVVGGGNSAAQIVAEVSTVAETLWLTSREPRFLPDDVDGRILFDVAREHAREIASGETDSGGIAGLGDI
VMVPPVQAARERGVMVAHPMFDRLTATGLGFGDHVEQVDAIIWCTGFRPALRHLQPLGLRDGDTGRIELDGAHVAGEPGL
IFLGYGDWTGPASATLIGVGRTARDAITALDDDLFTVPAI
>Mature_360_residues
MHSQGTPITVDVVVIGGGQAGMAAGYYLRRSGRDFVILDAETAPGGSWQHYWDSLHLFSPAEYSSLPGWPMPGWSPGFPP
ASHVVDYLTAYETRYDLPVRRPVRVTSVTRSGDGYLVESDHGSWSARAVVNTTGTWRQPFWPIYPGIREFTGTQIHTTSY
RSPTPFAGSKVAVVGGGNSAAQIVAEVSTVAETLWLTSREPRFLPDDVDGRILFDVAREHAREIASGETDSGGIAGLGDI
VMVPPVQAARERGVMVAHPMFDRLTATGLGFGDHVEQVDAIIWCTGFRPALRHLQPLGLRDGDTGRIELDGAHVAGEPGL
IFLGYGDWTGPASATLIGVGRTARDAITALDDDLFTVPAI

Specific function: Involved in potassium and divalent cation transport. Enhances the transport activity of the cation/potassium transporter CzcD [H]

COG id: COG2072

COG function: function code P; Predicted flavoprotein involved in K+ transport

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4503755, Length=210, Percent_Identity=27.1428571428571, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI221316672, Length=211, Percent_Identity=25.5924170616114, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI221316674, Length=211, Percent_Identity=25.5924170616114, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI221316678, Length=211, Percent_Identity=25.5924170616114, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: 808 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR000103 [H]

Pfam domain/function: PF07992 Pyr_redox_2 [H]

EC number: 1.8.1.9 [C]

Molecular weight: Translated: 38696; Mature: 38696

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSQGTPITVDVVVIGGGQAGMAAGYYLRRSGRDFVILDAETAPGGSWQHYWDSLHLFSP
CCCCCCCEEEEEEEECCCCCCHHHCEEEEECCCEEEEEECCCCCCCCHHHHHCCEEEECC
AEYSSLPGWPMPGWSPGFPPASHVVDYLTAYETRYDLPVRRPVRVTSVTRSGDGYLVESD
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCEEEECC
HGSWSARAVVNTTGTWRQPFWPIYPGIREFTGTQIHTTSYRSPTPFAGSKVAVVGGGNSA
CCCEEEEEEEECCCCCCCCCCCCCCCHHHHCCCEEEECCCCCCCCCCCCEEEEEECCCHH
AQIVAEVSTVAETLWLTSREPRFLPDDVDGRILFDVAREHAREIASGETDSGGIAGLGDI
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCCCE
VMVPPVQAARERGVMVAHPMFDRLTATGLGFGDHVEQVDAIIWCTGFRPALRHLQPLGLR
EEECCHHHHHHCCEEEECCHHHHHHHCCCCCCHHHHHEEEEEEECCCHHHHHCCCCCCCC
DGDTGRIELDGAHVAGEPGLIFLGYGDWTGPASATLIGVGRTARDAITALDDDLFTVPAI
CCCCCEEEECCCEECCCCCEEEEECCCCCCCCCEEEEECCCHHHHHHHHHCCCCEECCCC
>Mature Secondary Structure
MHSQGTPITVDVVVIGGGQAGMAAGYYLRRSGRDFVILDAETAPGGSWQHYWDSLHLFSP
CCCCCCCEEEEEEEECCCCCCHHHCEEEEECCCEEEEEECCCCCCCCHHHHHCCEEEECC
AEYSSLPGWPMPGWSPGFPPASHVVDYLTAYETRYDLPVRRPVRVTSVTRSGDGYLVESD
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCEEEECC
HGSWSARAVVNTTGTWRQPFWPIYPGIREFTGTQIHTTSYRSPTPFAGSKVAVVGGGNSA
CCCEEEEEEEECCCCCCCCCCCCCCCHHHHCCCEEEECCCCCCCCCCCCEEEEEECCCHH
AQIVAEVSTVAETLWLTSREPRFLPDDVDGRILFDVAREHAREIASGETDSGGIAGLGDI
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCCCE
VMVPPVQAARERGVMVAHPMFDRLTATGLGFGDHVEQVDAIIWCTGFRPALRHLQPLGLR
EEECCHHHHHHCCEEEECCHHHHHHHCCCCCCHHHHHEEEEEEECCCHHHHHCCCCCCCC
DGDTGRIELDGAHVAGEPGLIFLGYGDWTGPASATLIGVGRTARDAITALDDDLFTVPAI
CCCCCEEEECCCEECCCCCEEEEECCCCCCCCCEEEEECCCHHHHHHHHHCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: NA

Kcat value (1/min): 1365 [C]

Specific activity: NA

Km value (mM): 0.008 {NADPH}} 0.125 {thioredoxin} 0.002 {thioredoxin}} 0.0017 {thioredoxin}} [C]

Substrates: NADPH; oxidized thioredoxin [C]

Specific reaction: NADPH + oxidized thioredoxin = NADP+ + reduced thioredoxin [C]

General reaction: Redox reaction [C]

Inhibitor: Ca2+; N-Ethyl maleimide; NADP+ [C]

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9099864; 9308178; 9384377 [H]