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The map label for this gene is rbmK [H]

Identifier: 76818207

GI number: 76818207

Start: 21862

End: 23304

Strand: Direct

Name: rbmK [H]

Synonym: BURPS1710b_A0004

Alternate gene names: 76818207

Gene position: 21862-23304 (Clockwise)

Preceding gene: 76818976

Following gene: 76817385

Centisome position: 0.69

GC content: 71.59

Gene sequence:

>1443_bases
ATGACATCCGAGATTTCCGGCAAACGGCGCTGGTATGCGCTCGTCGTTCTGTGTCTGGGCGTGCTGATGATCGTGCTCGA
CACGACCATCGTGAACGTCGCGCTGCCGTCGATCGCGGCCGAGCTGCGTTTTTCCGAGAACGCGCTCGTCTGGGTCGTCA
ACGCCTACATGCTGACGTTCGGCGGCTGCCTGCTGCTCGGCGGCAGGCTCGGCGACCTGTACGGGCACCGGCGCCTCTTC
CTGGCCGGCGTGACGGTGTTCACGGCCGCGTCGCTCGCCTGCGGGCTCGCGCAGACGCAACTCGTGCTGATCGCCGCGCG
CGCCGCGCAGGGCATCGGCGGCGCGGTCGTCTCCTCGGTCGCGCTGTCGCTCATCATGACGCTGTTCGTCGAGCCGGGCG
ACCGGGCGAAGGCGATGGGCGTCTACGGCTTCGTCTATGCGGGCGGCGGCAGCCTCGGCGTGCTGCTCGGCGGGCTCCTG
ACGAGCGCGTTGAGCTGGCACTGGGTCTTTCTCGTCAACCTGCCGCTCGGCGCCGTGGTCTATCTGCTGTCGTCGGCGCT
GCTGCCGAAGGCGCGCGCGCAGGCAACGCGCGCGCCGCTCGACATCGCCGGCGCCGTGACCGTGACCGCCGCGCTGATGT
TGGCCGTCTACGCGGTGGTGAACGGCAACGCGGCCGGCTGGCTGTCCGCGCGCACGCTGAGCTGGCTCGGCGGCGCGGCG
GCGCTGTTCGCCGCGTTCGTCGCGATCGAGGCGCGCGTCGCGCATCCGCTGATGCCGCTCGCGCTCTGCATGAAGCGCAA
CGTCGCGATCGCGAACCTGATCGGCATCCTGTGGGCCGCCGCGATGTTCGCGTGGTTCTTCCTCGCCGCGCTGTATCTGC
AGCGCGTGCTCGGCTACGGGCCGTTGCAGATCGGCCTCGCGTTCCTGCCGGCGAACCTGATCATGGGCGTGTTCTCGCTC
ATCGTCTCCGCGCTGCTCGTGGTGCGCTTCGGGATCCGCGTGCCGATCGCGGCCGGACTGCTCGCGGCGGCGGCCGGCCT
GATGCTGTTCGCTCGGGCGCCCGTCGGCGGGGACTTCGTCGCCGACGTGCTGCCGGGCATGATCCTGCTGGGCACCGGCG
CGGGCATCGCGTTCAACCCGGTCCTGCTCGCGGCGATGAGCGGCGTCGATCCGGCGGACTCGGGGCTCGCCTCGGGCATC
GTCAACACGTCGTTCATGATGGGCGGCGCGCTCGGCCTCGCGGTGCTCGCGAGCGTCGCGACGGCGCGCACCGATGCGCT
GCTCGCCGCGCGCACGGCCGTGCCCGCCGCGATGGCGGGCGGCTACCATGCGGCGTTCATGCTCGGTGCGGCGTTCGCGA
TCGTCGCCGCGCTGATCGGGATGCGGCTGCGGGCCGCCCCGAATCCGGCGGCGATGCAGGGGCTGCAAGGCGCGACGCAG
TAG

Upstream 100 bases:

>100_bases
GACGTCGCGTGAGGCCGCGCCGCGCGCCGGCGCCGCGCGGCGTCGGCCGCCGCCGCCGCCGCCGCGTGCGCATCCCCCAT
CACTTCATGGAGTTCCATCG

Downstream 100 bases:

>100_bases
CGCGGTCCCGTCGCGCGGCGAGCTTGGTCGGTGTGTTCGACCGGCGAGTTCGGTCGGTGTGTTCGACCGGCAAGTGTGAT
CGACGAATTTGATCGGTGTG

Product: EmrB/QacA family drug resistance transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MTSEISGKRRWYALVVLCLGVLMIVLDTTIVNVALPSIAAELRFSENALVWVVNAYMLTFGGCLLLGGRLGDLYGHRRLF
LAGVTVFTAASLACGLAQTQLVLIAARAAQGIGGAVVSSVALSLIMTLFVEPGDRAKAMGVYGFVYAGGGSLGVLLGGLL
TSALSWHWVFLVNLPLGAVVYLLSSALLPKARAQATRAPLDIAGAVTVTAALMLAVYAVVNGNAAGWLSARTLSWLGGAA
ALFAAFVAIEARVAHPLMPLALCMKRNVAIANLIGILWAAAMFAWFFLAALYLQRVLGYGPLQIGLAFLPANLIMGVFSL
IVSALLVVRFGIRVPIAAGLLAAAAGLMLFARAPVGGDFVADVLPGMILLGTGAGIAFNPVLLAAMSGVDPADSGLASGI
VNTSFMMGGALGLAVLASVATARTDALLAARTAVPAAMAGGYHAAFMLGAAFAIVAALIGMRLRAAPNPAAMQGLQGATQ

Sequences:

>Translated_480_residues
MTSEISGKRRWYALVVLCLGVLMIVLDTTIVNVALPSIAAELRFSENALVWVVNAYMLTFGGCLLLGGRLGDLYGHRRLF
LAGVTVFTAASLACGLAQTQLVLIAARAAQGIGGAVVSSVALSLIMTLFVEPGDRAKAMGVYGFVYAGGGSLGVLLGGLL
TSALSWHWVFLVNLPLGAVVYLLSSALLPKARAQATRAPLDIAGAVTVTAALMLAVYAVVNGNAAGWLSARTLSWLGGAA
ALFAAFVAIEARVAHPLMPLALCMKRNVAIANLIGILWAAAMFAWFFLAALYLQRVLGYGPLQIGLAFLPANLIMGVFSL
IVSALLVVRFGIRVPIAAGLLAAAAGLMLFARAPVGGDFVADVLPGMILLGTGAGIAFNPVLLAAMSGVDPADSGLASGI
VNTSFMMGGALGLAVLASVATARTDALLAARTAVPAAMAGGYHAAFMLGAAFAIVAALIGMRLRAAPNPAAMQGLQGATQ
>Mature_479_residues
TSEISGKRRWYALVVLCLGVLMIVLDTTIVNVALPSIAAELRFSENALVWVVNAYMLTFGGCLLLGGRLGDLYGHRRLFL
AGVTVFTAASLACGLAQTQLVLIAARAAQGIGGAVVSSVALSLIMTLFVEPGDRAKAMGVYGFVYAGGGSLGVLLGGLLT
SALSWHWVFLVNLPLGAVVYLLSSALLPKARAQATRAPLDIAGAVTVTAALMLAVYAVVNGNAAGWLSARTLSWLGGAAA
LFAAFVAIEARVAHPLMPLALCMKRNVAIANLIGILWAAAMFAWFFLAALYLQRVLGYGPLQIGLAFLPANLIMGVFSLI
VSALLVVRFGIRVPIAAGLLAAAAGLMLFARAPVGGDFVADVLPGMILLGTGAGIAFNPVLLAAMSGVDPADSGLASGIV
NTSFMMGGALGLAVLASVATARTDALLAARTAVPAAMAGGYHAAFMLGAAFAIVAALIGMRLRAAPNPAAMQGLQGATQ

Specific function: May be involved in active puromycin efflux energized by a proton-dependent electrochemical gradient. In addition, it could be implicated in secreting N-acetylpuromycin, the last intermediate of the puromycin biosynthesis pathway, to the environment [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI87081983, Length=418, Percent_Identity=29.6650717703349, Blast_Score=143, Evalue=3e-35,
Organism=Escherichia coli, GI1789042, Length=423, Percent_Identity=26.4775413711584, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1788710, Length=420, Percent_Identity=25.952380952381, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1788392, Length=359, Percent_Identity=30.08356545961, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1790195, Length=255, Percent_Identity=31.3725490196078, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324954, Length=390, Percent_Identity=25.3846153846154, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6323520, Length=444, Percent_Identity=25, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6325455, Length=133, Percent_Identity=30.8270676691729, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6321663, Length=152, Percent_Identity=30.9210526315789, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322958, Length=387, Percent_Identity=23.5142118863049, Blast_Score=67, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6319770, Length=392, Percent_Identity=23.469387755102, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 48977; Mature: 48846

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSEISGKRRWYALVVLCLGVLMIVLDTTIVNVALPSIAAELRFSENALVWVVNAYMLTF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEHHHHHHHHH
GGCLLLGGRLGDLYGHRRLFLAGVTVFTAASLACGLAQTQLVLIAARAAQGIGGAVVSSV
CCHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
ALSLIMTLFVEPGDRAKAMGVYGFVYAGGGSLGVLLGGLLTSALSWHWVFLVNLPLGAVV
HHHHHHHHHCCCCCCCHHEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHEEEECCHHHHH
YLLSSALLPKARAQATRAPLDIAGAVTVTAALMLAVYAVVNGNAAGWLSARTLSWLGGAA
HHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
ALFAAFVAIEARVAHPLMPLALCMKRNVAIANLIGILWAAAMFAWFFLAALYLQRVLGYG
HHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PLQIGLAFLPANLIMGVFSLIVSALLVVRFGIRVPIAAGLLAAAAGLMLFARAPVGGDFV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHH
ADVLPGMILLGTGAGIAFNPVLLAAMSGVDPADSGLASGIVNTSFMMGGALGLAVLASVA
HHHHCCEEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TARTDALLAARTAVPAAMAGGYHAAFMLGAAFAIVAALIGMRLRAAPNPAAMQGLQGATQ
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCC
>Mature Secondary Structure 
TSEISGKRRWYALVVLCLGVLMIVLDTTIVNVALPSIAAELRFSENALVWVVNAYMLTF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEHHHHHHHHH
GGCLLLGGRLGDLYGHRRLFLAGVTVFTAASLACGLAQTQLVLIAARAAQGIGGAVVSSV
CCHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
ALSLIMTLFVEPGDRAKAMGVYGFVYAGGGSLGVLLGGLLTSALSWHWVFLVNLPLGAVV
HHHHHHHHHCCCCCCCHHEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHEEEECCHHHHH
YLLSSALLPKARAQATRAPLDIAGAVTVTAALMLAVYAVVNGNAAGWLSARTLSWLGGAA
HHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
ALFAAFVAIEARVAHPLMPLALCMKRNVAIANLIGILWAAAMFAWFFLAALYLQRVLGYG
HHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PLQIGLAFLPANLIMGVFSLIVSALLVVRFGIRVPIAAGLLAAAAGLMLFARAPVGGDFV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHH
ADVLPGMILLGTGAGIAFNPVLLAAMSGVDPADSGLASGIVNTSFMMGGALGLAVLASVA
HHHHCCEEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
TARTDALLAARTAVPAAMAGGYHAAFMLGAAFAIVAALIGMRLRAAPNPAAMQGLQGATQ
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7916693 [H]