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The map label for this gene is ilvD

Identifier: 75674412

GI number: 75674412

Start: 243124

End: 244968

Strand: Direct

Name: ilvD

Synonym: Nwi_0213

Alternate gene names: 75674412

Gene position: 243124-244968 (Clockwise)

Preceding gene: 75674411

Following gene: 75674413

Centisome position: 7.15

GC content: 65.47

Gene sequence:

>1845_bases
ATGCCCGCCTATCGCTCCCGTACCTCCACCCACGGCCGTAACATGGCCGGCGCCCGCGGCCTGTGGCGCGCCACCGGCAT
GAAGAACGAAGATTTCGGCAAGCCGATCATTGCGGTGGTCAACTCCTTCACCCAGTTCGTGCCCGGCCACGTTCACCTCA
AGGACCTCGGCCAGCTGGTGGCGCGCGAAATCGAGAAGGCCGGCGGCGTCGCCAAGGAATTCCATACCATCGCGGTGGAT
GACGGCATCGCGATGGGCCATGACGGGATGCTCTACAGCCTGCCCTCGCGCGAGGTCATCGCCGACAGCGTCGAATACAT
GGTCAACGCGCATTGCGCCGACGCCATGGTCTGCATCTCCAATTGCGACAAGATCACGCCCGGCATGCTGATGGCGTCGC
TGCGCCTCAACATACCCTCGGTGTTCGTCTCCGGAGGCCCGATGGAATCGGGCAAGGTCACGCTCAACGGCAAGGTCAAG
TCCGTCGACCTGATCGACGCCATGGTCGCCGCCGCCGATGACAGCGTGAGCGATGCCGACGTCGAGGCGATCGAACGCTC
GGCGTGCCCGACCTGCGGTTCATGCTCGGGCATGTTCACCGCCAATTCGATGAACTGCCTGACCGAGGCGCTCGGACTTG
CCCTGCCCGGCAACGGCTCGGTGCTCGCCACCCACGCCGACCGCAAGGGCCTGTTCGTCGAGGCCGGACATCTCATCGTC
GATATGGCGCGGCGCTACTACGAGCAGAACGACGAGCGTGTGCTGCCGCGTTCGGTCGCGAGCTTCAAGGCGTTCGAGAA
TGCGATGACGCTGGACATCTCGATGGGCGGCTCCACCAACACGGTGCTGCATCTGCTCGCCGCGGCTTACGAAGGCGAGG
TGCCGTTCACGATGGCGGATATCGACCGGCTGTCGCGGCGCGTGCCGGTCCTGTGCAAGGTCGCGCCCTCGGTCGCGGAC
GTGCATCTGGAAGACGTGCATCGCGCCGGCGGCATCATGGGCATTCTCGGCGAACTCGATCGCGCCGGACTGATCGACGC
CAGCCTGCCGACGGTCCACAGCAACTCGCTGAGGGAAGCCCTGGAACGCTGGGACATCAAGCGGACAAAAAGCGAGAGCG
TGCGCACCTTCTACACCGCCGCGCCGGGAGGCGTCCGCACCGAAATCGCATTCAGCCAGGACAAGCGGTTCGAGGAGCTC
GACGCCGACCGCGCCAAAGGCTGCATCCGCGATGCCGAACACGCCTTCTCGAAGGACGGCGGTCTCGCCGTGCTCTACGG
CAACATCGCGCGCGACGGCTGCATCGTGAAGACGGCCGGCGTTGACGACAGCATCCTGACCTTCTCAGGCCCCGCGCGCG
TGTTCGAAAGTCAGGATGCCGCGGTGGACGCCATCCTGGGCAACAGGATCAAGCCGGGCGATGTGGTGCTGATTCGCTAC
GAGGGACCGCGCGGCGGTCCGGGCATGCAGGAGATGCTGTATCCAACCAGCTATCTGAAATCGAAGGGACTGGGCAAGCA
GTGCGCGTTGATTACCGACGGTCGCTTCTCCGGCGGCTCATCGGGCCTGTCGATCGGCCACGTCTCGCCGGAAGCAGCGG
AGGGCGGCGCGATCGGTCTCGTCGAGGAAGGCGATCTCATCGCGTTCGACATTCCGAACCGGAAGGTTCATCTCGATGTC
AGCGATGCCGAACTCGAGCGACGCCGCGCGGCGATGGAAGCGAAAGGCGACAAGGCGTGGAAGCCGGCCGCTCCGCGCAC
GCGGCGCATCACCATGGCGCTGAAAGCCTACGCTGCGCATACCACCAGCGCCGCGCTCGGCGCCGTGCGCGTGGTGAAGG
ACTGA

Upstream 100 bases:

>100_bases
AAAACCATCGTGATCTAGTCGAGCGCAGCCACTTTTCTGTTTTGCGGCCTTCGGCGGCGGGGGTATGGTCCCGCCAAATC
ACTGACCGTTCGGAAAAAAC

Downstream 100 bases:

>100_bases
GCGTGCCACGTTCCGCCGCTGACCAAACCTGAGCATCTTCCGGCGAAATGGATGACCCGCTCGCCGCAAGAAAATGCGGT
CAGAAGATAATCTCCGGAAA

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD

Number of amino acids: Translated: 614; Mature: 613

Protein sequence:

>614_residues
MPAYRSRTSTHGRNMAGARGLWRATGMKNEDFGKPIIAVVNSFTQFVPGHVHLKDLGQLVAREIEKAGGVAKEFHTIAVD
DGIAMGHDGMLYSLPSREVIADSVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPSVFVSGGPMESGKVTLNGKVK
SVDLIDAMVAAADDSVSDADVEAIERSACPTCGSCSGMFTANSMNCLTEALGLALPGNGSVLATHADRKGLFVEAGHLIV
DMARRYYEQNDERVLPRSVASFKAFENAMTLDISMGGSTNTVLHLLAAAYEGEVPFTMADIDRLSRRVPVLCKVAPSVAD
VHLEDVHRAGGIMGILGELDRAGLIDASLPTVHSNSLREALERWDIKRTKSESVRTFYTAAPGGVRTEIAFSQDKRFEEL
DADRAKGCIRDAEHAFSKDGGLAVLYGNIARDGCIVKTAGVDDSILTFSGPARVFESQDAAVDAILGNRIKPGDVVLIRY
EGPRGGPGMQEMLYPTSYLKSKGLGKQCALITDGRFSGGSSGLSIGHVSPEAAEGGAIGLVEEGDLIAFDIPNRKVHLDV
SDAELERRRAAMEAKGDKAWKPAAPRTRRITMALKAYAAHTTSAALGAVRVVKD

Sequences:

>Translated_614_residues
MPAYRSRTSTHGRNMAGARGLWRATGMKNEDFGKPIIAVVNSFTQFVPGHVHLKDLGQLVAREIEKAGGVAKEFHTIAVD
DGIAMGHDGMLYSLPSREVIADSVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPSVFVSGGPMESGKVTLNGKVK
SVDLIDAMVAAADDSVSDADVEAIERSACPTCGSCSGMFTANSMNCLTEALGLALPGNGSVLATHADRKGLFVEAGHLIV
DMARRYYEQNDERVLPRSVASFKAFENAMTLDISMGGSTNTVLHLLAAAYEGEVPFTMADIDRLSRRVPVLCKVAPSVAD
VHLEDVHRAGGIMGILGELDRAGLIDASLPTVHSNSLREALERWDIKRTKSESVRTFYTAAPGGVRTEIAFSQDKRFEEL
DADRAKGCIRDAEHAFSKDGGLAVLYGNIARDGCIVKTAGVDDSILTFSGPARVFESQDAAVDAILGNRIKPGDVVLIRY
EGPRGGPGMQEMLYPTSYLKSKGLGKQCALITDGRFSGGSSGLSIGHVSPEAAEGGAIGLVEEGDLIAFDIPNRKVHLDV
SDAELERRRAAMEAKGDKAWKPAAPRTRRITMALKAYAAHTTSAALGAVRVVKD
>Mature_613_residues
PAYRSRTSTHGRNMAGARGLWRATGMKNEDFGKPIIAVVNSFTQFVPGHVHLKDLGQLVAREIEKAGGVAKEFHTIAVDD
GIAMGHDGMLYSLPSREVIADSVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPSVFVSGGPMESGKVTLNGKVKS
VDLIDAMVAAADDSVSDADVEAIERSACPTCGSCSGMFTANSMNCLTEALGLALPGNGSVLATHADRKGLFVEAGHLIVD
MARRYYEQNDERVLPRSVASFKAFENAMTLDISMGGSTNTVLHLLAAAYEGEVPFTMADIDRLSRRVPVLCKVAPSVADV
HLEDVHRAGGIMGILGELDRAGLIDASLPTVHSNSLREALERWDIKRTKSESVRTFYTAAPGGVRTEIAFSQDKRFEELD
ADRAKGCIRDAEHAFSKDGGLAVLYGNIARDGCIVKTAGVDDSILTFSGPARVFESQDAAVDAILGNRIKPGDVVLIRYE
GPRGGPGMQEMLYPTSYLKSKGLGKQCALITDGRFSGGSSGLSIGHVSPEAAEGGAIGLVEEGDLIAFDIPNRKVHLDVS
DAELERRRAAMEAKGDKAWKPAAPRTRRITMALKAYAAHTTSAALGAVRVVKD

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family

Homologues:

Organism=Escherichia coli, GI48994964, Length=610, Percent_Identity=72.9508196721311, Blast_Score=938, Evalue=0.0,
Organism=Escherichia coli, GI2367371, Length=615, Percent_Identity=31.219512195122, Blast_Score=191, Evalue=9e-50,
Organism=Escherichia coli, GI1788157, Length=539, Percent_Identity=29.3135435992579, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1786464, Length=516, Percent_Identity=31.7829457364341, Blast_Score=174, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6322476, Length=608, Percent_Identity=37.0065789473684, Blast_Score=343, Evalue=3e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ILVD_NITWN (Q3SW60)

Other databases:

- EMBL:   CP000115
- RefSeq:   YP_316833.1
- STRING:   Q3SW60
- GeneID:   3676670
- GenomeReviews:   CP000115_GR
- KEGG:   nwi:Nwi_0213
- NMPDR:   fig|323098.3.peg.683
- eggNOG:   COG0129
- HOGENOM:   HBG671001
- OMA:   KVPCLSK
- PhylomeDB:   Q3SW60
- ProtClustDB:   PRK12448
- BioCyc:   NWIN323098:NWI_0213-MONOMER
- HAMAP:   MF_00012
- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558
- PANTHER:   PTHR21000
- TIGRFAMs:   TIGR00110

Pfam domain/function: PF00920 ILVD_EDD; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.9

Molecular weight: Translated: 65467; Mature: 65336

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00886 ILVD_EDD_1; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAYRSRTSTHGRNMAGARGLWRATGMKNEDFGKPIIAVVNSFTQFVPGHVHLKDLGQLV
CCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHH
AREIEKAGGVAKEFHTIAVDDGIAMGHDGMLYSLPSREVIADSVEYMVNAHCADAMVCIS
HHHHHHHCCCHHHEEEEEECCCEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
NCDKITPGMLMASLRLNIPSVFVSGGPMESGKVTLNGKVKSVDLIDAMVAAADDSVSDAD
CCCCCCHHHHHHHHHCCCCEEEEECCCCCCCEEEECCCEEEHHHHHHHHHHCCCCCCCHH
VEAIERSACPTCGSCSGMFTANSMNCLTEALGLALPGNGSVLATHADRKGLFVEAGHLIV
HHHHHHHCCCCCCCCCCCEECCHHHHHHHHHCEEECCCCCEEEEECCCCCEEEECCHHHH
DMARRYYEQNDERVLPRSVASFKAFENAMTLDISMGGSTNTVLHLLAAAYEGEVPFTMAD
HHHHHHHHCCCCEECHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHCCCCCEEHHH
IDRLSRRVPVLCKVAPSVADVHLEDVHRAGGIMGILGELDRAGLIDASLPTVHSNSLREA
HHHHHCCCCEEEEECCCHHHHHHHHHHHHCCHHHHHHCCCCCCEEECCCCCCCCHHHHHH
LERWDIKRTKSESVRTFYTAAPGGVRTEIAFSQDKRFEELDADRAKGCIRDAEHAFSKDG
HHHCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCHHHHHCCHHHHHHHHHHHHHHCCCC
GLAVLYGNIARDGCIVKTAGVDDSILTFSGPARVFESQDAAVDAILGNRIKPGDVVLIRY
CEEEEECCCCCCCEEEEECCCCCCEEEECCCHHHHCCCCHHHHHHHCCCCCCCCEEEEEE
EGPRGGPGMQEMLYPTSYLKSKGLGKQCALITDGRFSGGSSGLSIGHVSPEAAEGGAIGL
CCCCCCCCHHHHHCCHHHHHHCCCCCEEEEEECCCCCCCCCCCEEECCCCCCCCCCEEEE
VEEGDLIAFDIPNRKVHLDVSDAELERRRAAMEAKGDKAWKPAAPRTRRITMALKAYAAH
EECCCEEEEECCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHH
TTSAALGAVRVVKD
HHHHHHHEEEEECC
>Mature Secondary Structure 
PAYRSRTSTHGRNMAGARGLWRATGMKNEDFGKPIIAVVNSFTQFVPGHVHLKDLGQLV
CCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHH
AREIEKAGGVAKEFHTIAVDDGIAMGHDGMLYSLPSREVIADSVEYMVNAHCADAMVCIS
HHHHHHHCCCHHHEEEEEECCCEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
NCDKITPGMLMASLRLNIPSVFVSGGPMESGKVTLNGKVKSVDLIDAMVAAADDSVSDAD
CCCCCCHHHHHHHHHCCCCEEEEECCCCCCCEEEECCCEEEHHHHHHHHHHCCCCCCCHH
VEAIERSACPTCGSCSGMFTANSMNCLTEALGLALPGNGSVLATHADRKGLFVEAGHLIV
HHHHHHHCCCCCCCCCCCEECCHHHHHHHHHCEEECCCCCEEEEECCCCCEEEECCHHHH
DMARRYYEQNDERVLPRSVASFKAFENAMTLDISMGGSTNTVLHLLAAAYEGEVPFTMAD
HHHHHHHHCCCCEECHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHCCCCCEEHHH
IDRLSRRVPVLCKVAPSVADVHLEDVHRAGGIMGILGELDRAGLIDASLPTVHSNSLREA
HHHHHCCCCEEEEECCCHHHHHHHHHHHHCCHHHHHHCCCCCCEEECCCCCCCCHHHHHH
LERWDIKRTKSESVRTFYTAAPGGVRTEIAFSQDKRFEELDADRAKGCIRDAEHAFSKDG
HHHCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCHHHHHCCHHHHHHHHHHHHHHCCCC
GLAVLYGNIARDGCIVKTAGVDDSILTFSGPARVFESQDAAVDAILGNRIKPGDVVLIRY
CEEEEECCCCCCCEEEEECCCCCCEEEECCCHHHHCCCCHHHHHHHCCCCCCCCEEEEEE
EGPRGGPGMQEMLYPTSYLKSKGLGKQCALITDGRFSGGSSGLSIGHVSPEAAEGGAIGL
CCCCCCCCHHHHHCCHHHHHHCCCCCEEEEEECCCCCCCCCCCEEECCCCCCCCCCEEEE
VEEGDLIAFDIPNRKVHLDVSDAELERRRAAMEAKGDKAWKPAAPRTRRITMALKAYAAH
EECCCEEEEECCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHH
TTSAALGAVRVVKD
HHHHHHHEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA