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The map label for this gene is grpE

Identifier: 75674394

GI number: 75674394

Start: 225153

End: 225746

Strand: Direct

Name: grpE

Synonym: Nwi_0195

Alternate gene names: 75674394

Gene position: 225153-225746 (Clockwise)

Preceding gene: 75674393

Following gene: 75674396

Centisome position: 6.62

GC content: 64.31

Gene sequence:

>594_bases
ATGACCGATTCCGACGGCAAAACAGACAAGTCCGGCGAACCGGCTGCGGAGGTCGAGCCGGTGGTATCCAAGCCGTATGT
CATGCCGGACGATCCCGAGGACGATGCGCTCGACGCGCTGAACAAGCAGCTCGCAGAGGCGAAAGATCGGACGTTGCGCA
CGCTGGCGGAGATGGAGAATCTGCGCAAGCGTACGGCGCGGGAGGTCTCGGACGCTCGAACCTACGGCATCTCGGGCTTC
GCGCGCGACGTTCTGGAGATCGCCGACAATCTGCAGCGCGCGCTTGATGCGGTGCCGGCGGACGCTCGCGCGGCGCCAGA
TCCCGGCCTGAAAGCCCTGATCGAGGGCGTGGAGCTGACCGAACGCTCGCTGCACAACGCTCTGGAAAAGCATGGCGTGA
AAAAATTTGATCCGGCGGGCGAAAAATTCGATCCCAACGTCCATCAGGCCATGTACGAAGTTCCCGATCCGTCGATCCCC
GTCGGCACGGTCGCCCAGGTCATTCAAGCCGGCTACATGATCGGAGAGCGGGTGCTGCGCCCGGCGCTGGTGGGCGTTGC
GAAGGGCGGAGCCAAGGCGGCCGCGCCGGAATAA

Upstream 100 bases:

>100_bases
CAGGTGAGCCGGACCCGCCACGCTTGATTTTTACGCCTGAACCACGATATCCCGCACGACCATTCCTTTCCATTCGAGAC
AATTGCGAGAAGGCTGCGTA

Downstream 100 bases:

>100_bases
TGGCAGCGCGCGTCGGATCAAGATCACGGAGCCACGCTTCTGATTTGAATCAGAAGCGTGAGAGGTTAACGCGGCCGGAT
ACCGTCACGAACCGCGCGGA

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 197; Mature: 196

Protein sequence:

>197_residues
MTDSDGKTDKSGEPAAEVEPVVSKPYVMPDDPEDDALDALNKQLAEAKDRTLRTLAEMENLRKRTAREVSDARTYGISGF
ARDVLEIADNLQRALDAVPADARAAPDPGLKALIEGVELTERSLHNALEKHGVKKFDPAGEKFDPNVHQAMYEVPDPSIP
VGTVAQVIQAGYMIGERVLRPALVGVAKGGAKAAAPE

Sequences:

>Translated_197_residues
MTDSDGKTDKSGEPAAEVEPVVSKPYVMPDDPEDDALDALNKQLAEAKDRTLRTLAEMENLRKRTAREVSDARTYGISGF
ARDVLEIADNLQRALDAVPADARAAPDPGLKALIEGVELTERSLHNALEKHGVKKFDPAGEKFDPNVHQAMYEVPDPSIP
VGTVAQVIQAGYMIGERVLRPALVGVAKGGAKAAAPE
>Mature_196_residues
TDSDGKTDKSGEPAAEVEPVVSKPYVMPDDPEDDALDALNKQLAEAKDRTLRTLAEMENLRKRTAREVSDARTYGISGFA
RDVLEIADNLQRALDAVPADARAAPDPGLKALIEGVELTERSLHNALEKHGVKKFDPAGEKFDPNVHQAMYEVPDPSIPV
GTVAQVIQAGYMIGERVLRPALVGVAKGGAKAAAPE

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=149, Percent_Identity=43.6241610738255, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI40255109, Length=154, Percent_Identity=35.7142857142857, Blast_Score=97, Evalue=1e-20,
Organism=Escherichia coli, GI1788967, Length=178, Percent_Identity=39.3258426966292, Blast_Score=108, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17552458, Length=151, Percent_Identity=37.7483443708609, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6324806, Length=154, Percent_Identity=40.9090909090909, Blast_Score=126, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24653432, Length=181, Percent_Identity=40.8839779005525, Blast_Score=138, Evalue=2e-33,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_NITWN (Q3SW78)

Other databases:

- EMBL:   CP000115
- RefSeq:   YP_316815.1
- ProteinModelPortal:   Q3SW78
- SMR:   Q3SW78
- STRING:   Q3SW78
- GeneID:   3676651
- GenomeReviews:   CP000115_GR
- KEGG:   nwi:Nwi_0195
- NMPDR:   fig|323098.3.peg.665
- eggNOG:   COG0576
- HOGENOM:   HBG732630
- OMA:   NLHQAVM
- PhylomeDB:   Q3SW78
- ProtClustDB:   PRK14141
- BioCyc:   NWIN323098:NWI_0195-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 21108; Mature: 20977

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDSDGKTDKSGEPAAEVEPVVSKPYVMPDDPEDDALDALNKQLAEAKDRTLRTLAEMEN
CCCCCCCCCCCCCCHHHHCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRKRTAREVSDARTYGISGFARDVLEIADNLQRALDAVPADARAAPDPGLKALIEGVELT
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
ERSLHNALEKHGVKKFDPAGEKFDPNVHQAMYEVPDPSIPVGTVAQVIQAGYMIGERVLR
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
PALVGVAKGGAKAAAPE
HHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TDSDGKTDKSGEPAAEVEPVVSKPYVMPDDPEDDALDALNKQLAEAKDRTLRTLAEMEN
CCCCCCCCCCCCCHHHHCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRKRTAREVSDARTYGISGFARDVLEIADNLQRALDAVPADARAAPDPGLKALIEGVELT
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
ERSLHNALEKHGVKKFDPAGEKFDPNVHQAMYEVPDPSIPVGTVAQVIQAGYMIGERVLR
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
PALVGVAKGGAKAAAPE
HHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA