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The map label for this gene is rpoN2 [H]

Identifier: 75674376

GI number: 75674376

Start: 202121

End: 203752

Strand: Direct

Name: rpoN2 [H]

Synonym: Nwi_0177

Alternate gene names: 75674376

Gene position: 202121-203752 (Clockwise)

Preceding gene: 75674375

Following gene: 75674377

Centisome position: 5.94

GC content: 64.09

Gene sequence:

>1632_bases
ATGGCGCTGACGCAGAAACTCGAATTTCGACAGTCCCAGTCGCTGGTGATGACGCCGCAGCTGATGCAGGCGATCAAGCT
GCTGCAACTGTCGAATCTGGACCTCTCAGCATATGTCGAGGAGGAGCTGGAGCGGAATCCGCTATTGGAGCGTGCCAGCG
ACGGCTCCGGCCCGCCGGCCGCCGAAGCAGCAAGCGATGTTGAATATTCAGAAGGCGACGAAGCGGCGTTCGAGGACCAC
GGCGGGGGCGAGCGCACCGGCGCTCAGGATCCGGCGTCCACGCCCGAGGAATGGATGAACCGCGATCTCGGCAGTCGTGC
GGAGATCGAGCAGACCTTCGATAGCGGTCTGGAGAATGTTTTTCCGGAAGAACCGGCCGAGGCGGCTGCGCGGACCGCGC
AGGACGCCGCGCCGAGCGCGTTCACGGAATGGGGAGGCGGTGCCTCGAGCGATGACAGCTACAATCTCGAAGCTTTCGTC
GCGGCGGAGATAACGCTCAGTGAACATCTCGCCGAACAGCTTGCGGTGGCTCTCACCTCGCCGGCGCAGCGCATGATCGG
ACGGTATCTGATCGATCTCGTCGACGAGGCTGGCTACCTGCCGGCCGACCTCGGCGAAGCCGGCGACAGGCTCGGCGCCG
GCGAAAGCGATGTGGCGGCCGCTGTCGCGGTATTGCAGACGTTCGATCCGCCCGGCATCTGCGCCCGCAGCCTGAGCGAA
TGCCTTGCGATCCAGTTGCGCGAGAAGAACCGCTACGATCCGGCGATGCAGGCGCTGGTCGAGCATCTCGAACTGCTGGC
GAGGCGCGACTTCGCGGCCTTGCGCAGGATCTGCGGCGTCGACGATGAAGACCTTGCCGAGATGATCGGCGAAATCCGTC
ATCTCGATCCCAAGCCGGGCTTGCGGTTCGGTGCCGCGCGCACGCAGTCGGTGGTGCCGGACGTGTATGTGCGTCCCGGC
CCGGACGGCGGCTGGCTCGTGGAACTCAACAGCGAGACGCTGCCGCGCGTACTCGTCAACCACACCTACTATGCCGAATT
GTCGAAGACGATCCGCAAGGACGGCGACAAAACCTATTTCACGGACTGTTTGCAAAACGCGACCTGGCTGGTCCGTGCGC
TCGATCAGCGCGCCCGCACCATTCTCAAGGTGGCGACCGAGATCGTGCGGCAGCAGGACGGCTTTTTCGCGCACGGCATC
GCCCATCTGAGACCGCTCAATCTCAGGACCGTGGCGGATGCGATCCAGATGCATGAATCCACCGTGTCCCGCGTCACCGC
CAACAAATACATGGCGACAAATCGTGGCATCTTCGAACTGAAATACTTCTTCACCGCTTCGATCGCCGCGGCCGACGGCG
GTTCCGCCCACTCGGCGGAAGCGGTCCGGCACCACATCAAGCGGCTGATCGATGCCGAATCGCCGTCGGCGATTCTGTCC
GACGACACCATCGTGGAACGGCTGCGCGAAGCCGGAATTGATATCGCACGCCGCACAGTGGCGAAGTATCGCGAAGCGCT
GCGAATTCCTTCCTCGGTGCAGCGCCGCCGTGAAAAGCAGAATCTGCCAGGCGGCGCGCGCGCCGAGACCAGGAAGCCGC
CGAACCGCCCGCGCGATGCCGCACCGGCTTGA

Upstream 100 bases:

>100_bases
GCGCCGCTGGTTGCCGGAAGCCGAATTCGTTCCGGTCAGGCGTGTACATCTGTCCGCAGAAAAGCTAAGCAAGAATCGGA
CCGTTTGGACCGATGCTGGC

Downstream 100 bases:

>100_bases
TTGCGCAACCGCGACGCCCGATAATCACTTCCCACTCATGATCGATGAGGCTTGTCATGACACTTCGAATTTCGGGCAAG
AGCATCAATGTCGGCGAGGC

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 543; Mature: 542

Protein sequence:

>543_residues
MALTQKLEFRQSQSLVMTPQLMQAIKLLQLSNLDLSAYVEEELERNPLLERASDGSGPPAAEAASDVEYSEGDEAAFEDH
GGGERTGAQDPASTPEEWMNRDLGSRAEIEQTFDSGLENVFPEEPAEAAARTAQDAAPSAFTEWGGGASSDDSYNLEAFV
AAEITLSEHLAEQLAVALTSPAQRMIGRYLIDLVDEAGYLPADLGEAGDRLGAGESDVAAAVAVLQTFDPPGICARSLSE
CLAIQLREKNRYDPAMQALVEHLELLARRDFAALRRICGVDDEDLAEMIGEIRHLDPKPGLRFGAARTQSVVPDVYVRPG
PDGGWLVELNSETLPRVLVNHTYYAELSKTIRKDGDKTYFTDCLQNATWLVRALDQRARTILKVATEIVRQQDGFFAHGI
AHLRPLNLRTVADAIQMHESTVSRVTANKYMATNRGIFELKYFFTASIAAADGGSAHSAEAVRHHIKRLIDAESPSAILS
DDTIVERLREAGIDIARRTVAKYREALRIPSSVQRRREKQNLPGGARAETRKPPNRPRDAAPA

Sequences:

>Translated_543_residues
MALTQKLEFRQSQSLVMTPQLMQAIKLLQLSNLDLSAYVEEELERNPLLERASDGSGPPAAEAASDVEYSEGDEAAFEDH
GGGERTGAQDPASTPEEWMNRDLGSRAEIEQTFDSGLENVFPEEPAEAAARTAQDAAPSAFTEWGGGASSDDSYNLEAFV
AAEITLSEHLAEQLAVALTSPAQRMIGRYLIDLVDEAGYLPADLGEAGDRLGAGESDVAAAVAVLQTFDPPGICARSLSE
CLAIQLREKNRYDPAMQALVEHLELLARRDFAALRRICGVDDEDLAEMIGEIRHLDPKPGLRFGAARTQSVVPDVYVRPG
PDGGWLVELNSETLPRVLVNHTYYAELSKTIRKDGDKTYFTDCLQNATWLVRALDQRARTILKVATEIVRQQDGFFAHGI
AHLRPLNLRTVADAIQMHESTVSRVTANKYMATNRGIFELKYFFTASIAAADGGSAHSAEAVRHHIKRLIDAESPSAILS
DDTIVERLREAGIDIARRTVAKYREALRIPSSVQRRREKQNLPGGARAETRKPPNRPRDAAPA
>Mature_542_residues
ALTQKLEFRQSQSLVMTPQLMQAIKLLQLSNLDLSAYVEEELERNPLLERASDGSGPPAAEAASDVEYSEGDEAAFEDHG
GGERTGAQDPASTPEEWMNRDLGSRAEIEQTFDSGLENVFPEEPAEAAARTAQDAAPSAFTEWGGGASSDDSYNLEAFVA
AEITLSEHLAEQLAVALTSPAQRMIGRYLIDLVDEAGYLPADLGEAGDRLGAGESDVAAAVAVLQTFDPPGICARSLSEC
LAIQLREKNRYDPAMQALVEHLELLARRDFAALRRICGVDDEDLAEMIGEIRHLDPKPGLRFGAARTQSVVPDVYVRPGP
DGGWLVELNSETLPRVLVNHTYYAELSKTIRKDGDKTYFTDCLQNATWLVRALDQRARTILKVATEIVRQQDGFFAHGIA
HLRPLNLRTVADAIQMHESTVSRVTANKYMATNRGIFELKYFFTASIAAADGGSAHSAEAVRHHIKRLIDAESPSAILSD
DTIVERLREAGIDIARRTVAKYREALRIPSSVQRRREKQNLPGGARAETRKPPNRPRDAAPA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the nitrogen fixation genes [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=522, Percent_Identity=36.3984674329502, Blast_Score=314, Evalue=1e-86,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 59468; Mature: 59337

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALTQKLEFRQSQSLVMTPQLMQAIKLLQLSNLDLSAYVEEELERNPLLERASDGSGPPA
CCCCHHHHHHHCCCEEECHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHCCCCCCCCH
AEAASDVEYSEGDEAAFEDHGGGERTGAQDPASTPEEWMNRDLGSRAEIEQTFDSGLENV
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHC
FPEEPAEAAARTAQDAAPSAFTEWGGGASSDDSYNLEAFVAAEITLSEHLAEQLAVALTS
CCCCHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHCC
PAQRMIGRYLIDLVDEAGYLPADLGEAGDRLGAGESDVAAAVAVLQTFDPPGICARSLSE
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
CLAIQLREKNRYDPAMQALVEHLELLARRDFAALRRICGVDDEDLAEMIGEIRHLDPKPG
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCC
LRFGAARTQSVVPDVYVRPGPDGGWLVELNSETLPRVLVNHTYYAELSKTIRKDGDKTYF
CCCCCHHHHHCCCCCEEEECCCCCEEEEECCCHHHHHHHCCHHHHHHHHHHHCCCCHHHH
TDCLQNATWLVRALDQRARTILKVATEIVRQQDGFFAHGIAHLRPLNLRTVADAIQMHES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHH
TVSRVTANKYMATNRGIFELKYFFTASIAAADGGSAHSAEAVRHHIKRLIDAESPSAILS
HHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
DDTIVERLREAGIDIARRTVAKYREALRIPSSVQRRREKQNLPGGARAETRKPPNRPRDA
CHHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
APA
CCC
>Mature Secondary Structure 
ALTQKLEFRQSQSLVMTPQLMQAIKLLQLSNLDLSAYVEEELERNPLLERASDGSGPPA
CCCHHHHHHHCCCEEECHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHCCCCCCCCH
AEAASDVEYSEGDEAAFEDHGGGERTGAQDPASTPEEWMNRDLGSRAEIEQTFDSGLENV
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHC
FPEEPAEAAARTAQDAAPSAFTEWGGGASSDDSYNLEAFVAAEITLSEHLAEQLAVALTS
CCCCHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHCC
PAQRMIGRYLIDLVDEAGYLPADLGEAGDRLGAGESDVAAAVAVLQTFDPPGICARSLSE
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
CLAIQLREKNRYDPAMQALVEHLELLARRDFAALRRICGVDDEDLAEMIGEIRHLDPKPG
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCC
LRFGAARTQSVVPDVYVRPGPDGGWLVELNSETLPRVLVNHTYYAELSKTIRKDGDKTYF
CCCCCHHHHHCCCCCEEEECCCCCEEEEECCCHHHHHHHCCHHHHHHHHHHHCCCCHHHH
TDCLQNATWLVRALDQRARTILKVATEIVRQQDGFFAHGIAHLRPLNLRTVADAIQMHES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHH
TVSRVTANKYMATNRGIFELKYFFTASIAAADGGSAHSAEAVRHHIKRLIDAESPSAILS
HHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
DDTIVERLREAGIDIARRTVAKYREALRIPSSVQRRREKQNLPGGARAETRKPPNRPRDA
CHHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
APA
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1991712; 12597275 [H]