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The map label for this gene is purH [H]

Identifier: 75674357

GI number: 75674357

Start: 183485

End: 185077

Strand: Direct

Name: purH [H]

Synonym: Nwi_0158

Alternate gene names: 75674357

Gene position: 183485-185077 (Clockwise)

Preceding gene: 75674356

Following gene: 75674358

Centisome position: 5.39

GC content: 65.85

Gene sequence:

>1593_bases
ATGACCGACCGACCCCGCCGCGTGACCCGCGCCTTGCTTTCCGTTTCCGACAAGACCGGCCTGACCGAGTTCGCCCGCGC
GCTTGCCGACCTCGGCGTCGAACTGGTCTCGACCGGCGGCACCGCCAAGGAAATCGCGGCGGCGGGATTGAAGGTCAGTG
ACGTCTCCGACCTGACGGGTTTTCCCGAAATGATGGACGGCCGGGTCAAGACGCTGCATCCGAAGGTGCATGGCGGCCTG
CTCGCCATCCGCGACAACGCCGATCATGCGAAGTCCATGAAGGACCACGGCATCGCCCCGATCGATCTTTTGGTCGTCAA
TCTCTATCCGTTCGAATCAACGGTCGATAAAGGCGCCGCCTGCGAGGAGTGCATCGAGAATATCGACATCGGCGGCCCCG
CGATGATTCGCGCCGCCGCCAAGAACCATGATGACGTAGCGGTCGTGGTCGAACCGCAGGATTATCAGGCGGTGCTTGAC
GAACTCAAGGCCAACGCGGGCGCGACGACGTTGAGCTTGCGCAAGCGCCTCGCCGCGAAAGCCTACGCCCGGACCGCCGC
CTATGATGCGGCGATCTCCAACTGGTTCGCGGTGCAGCTTGAGACCGATGCGCCCGACTATCGCGCCGTCGGCGGCCGTC
TCGCGCAGAGGTTGCGCTATGGCGAGAATCCGCACCAGACCGCCGCGTTCTATCGCACGCCGGAGCGGCGCGCCGGCGTA
GCCACCGCGCGGCAGTTGCAGGGCAAGGAACTGTCCTACAACAACATCAACGACACCGACGCGGCTTACGAGTGCGTCGC
CGAATTCGATGCGGCGCGCACCGCGGCCTGCGTCATCATCAAGCACGCCAATCCCTGCGGTGTCGCGGAAGGCTCAAGCC
TCACCGAAGCCTATCGCCGGGCGCTCGCCTGTGACCAGACCTCGGCCTATGGCGGCATCATAGCCTTCAACCGCACCATC
GACGCCGACGCCGCCAATGCGGTGGCCGGCATCTTCACCGAAGTCATCATCGCGCCCGATGCGACCGAGGAGGCGATCGC
GGTCATCGGCAAGCGCAAGAACCTGCGGCTGCTGCTGGCCGGCGGCCTGCCCGATCCGCGCGCGCGCGGCCTGACCGCGA
AGACGGTCGCCGGCGGGCTTCTGGTGCAGGGCCGTGACAACGCCGTCATTGATGATATGTCACTGAAGGTCGTCACGAAG
CGTCCGCCGACCGAGGCGGAGATGCGCGACCTGCGGTTCGCCTTCCGTGTCGCCAAGCACGTCAAGTCGAACACCATCGT
CTATGCCAGGGATCTCGCCACCGTCGGCATCGGCGCGGGCCAGATGAGCCGCGTCGATTCCGCGCGCATCGCCGCGCGCA
AGGCGGAAGATGCGGCGCGCGATCTGAAGCTCGCCGAGCCCTTGACCAAAGGCTCGGTCGTGGCGTCGGATGCGTTCTTT
CCCTTCGCCGACGGCATGCTCGCCTGTATCAAAGCCGGCGCCACCGCGGTCATCCAGCCCGGCGGCTCCATGCGCGACGA
GGAGGTGATCAAGGCCGCCGACGAGCATGGCATCGCCATGGTGTTCACCGGCGTCAGGCATTTCCGTCATTAG

Upstream 100 bases:

>100_bases
CATTCCGTCCGTGGTCGGCGCCTGCACATCGGACAACGCCGGTTTCATGCGCTGCGAAATCATGTTAACGGGCGGCCTTG
ATTTCCCCAGGATCGTCCTC

Downstream 100 bases:

>100_bases
AGCGGCCGCCGGTCAAATGGAATCAGCGCCGCCGCTCCAGTTAATTGATTGAGCATCGGATTTATCCCGAAACCGGTTCC
CACTTTCGGGTCCGATGCTC

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MTDRPRRVTRALLSVSDKTGLTEFARALADLGVELVSTGGTAKEIAAAGLKVSDVSDLTGFPEMMDGRVKTLHPKVHGGL
LAIRDNADHAKSMKDHGIAPIDLLVVNLYPFESTVDKGAACEECIENIDIGGPAMIRAAAKNHDDVAVVVEPQDYQAVLD
ELKANAGATTLSLRKRLAAKAYARTAAYDAAISNWFAVQLETDAPDYRAVGGRLAQRLRYGENPHQTAAFYRTPERRAGV
ATARQLQGKELSYNNINDTDAAYECVAEFDAARTAACVIIKHANPCGVAEGSSLTEAYRRALACDQTSAYGGIIAFNRTI
DADAANAVAGIFTEVIIAPDATEEAIAVIGKRKNLRLLLAGGLPDPRARGLTAKTVAGGLLVQGRDNAVIDDMSLKVVTK
RPPTEAEMRDLRFAFRVAKHVKSNTIVYARDLATVGIGAGQMSRVDSARIAARKAEDAARDLKLAEPLTKGSVVASDAFF
PFADGMLACIKAGATAVIQPGGSMRDEEVIKAADEHGIAMVFTGVRHFRH

Sequences:

>Translated_530_residues
MTDRPRRVTRALLSVSDKTGLTEFARALADLGVELVSTGGTAKEIAAAGLKVSDVSDLTGFPEMMDGRVKTLHPKVHGGL
LAIRDNADHAKSMKDHGIAPIDLLVVNLYPFESTVDKGAACEECIENIDIGGPAMIRAAAKNHDDVAVVVEPQDYQAVLD
ELKANAGATTLSLRKRLAAKAYARTAAYDAAISNWFAVQLETDAPDYRAVGGRLAQRLRYGENPHQTAAFYRTPERRAGV
ATARQLQGKELSYNNINDTDAAYECVAEFDAARTAACVIIKHANPCGVAEGSSLTEAYRRALACDQTSAYGGIIAFNRTI
DADAANAVAGIFTEVIIAPDATEEAIAVIGKRKNLRLLLAGGLPDPRARGLTAKTVAGGLLVQGRDNAVIDDMSLKVVTK
RPPTEAEMRDLRFAFRVAKHVKSNTIVYARDLATVGIGAGQMSRVDSARIAARKAEDAARDLKLAEPLTKGSVVASDAFF
PFADGMLACIKAGATAVIQPGGSMRDEEVIKAADEHGIAMVFTGVRHFRH
>Mature_529_residues
TDRPRRVTRALLSVSDKTGLTEFARALADLGVELVSTGGTAKEIAAAGLKVSDVSDLTGFPEMMDGRVKTLHPKVHGGLL
AIRDNADHAKSMKDHGIAPIDLLVVNLYPFESTVDKGAACEECIENIDIGGPAMIRAAAKNHDDVAVVVEPQDYQAVLDE
LKANAGATTLSLRKRLAAKAYARTAAYDAAISNWFAVQLETDAPDYRAVGGRLAQRLRYGENPHQTAAFYRTPERRAGVA
TARQLQGKELSYNNINDTDAAYECVAEFDAARTAACVIIKHANPCGVAEGSSLTEAYRRALACDQTSAYGGIIAFNRTID
ADAANAVAGIFTEVIIAPDATEEAIAVIGKRKNLRLLLAGGLPDPRARGLTAKTVAGGLLVQGRDNAVIDDMSLKVVTKR
PPTEAEMRDLRFAFRVAKHVKSNTIVYARDLATVGIGAGQMSRVDSARIAARKAEDAARDLKLAEPLTKGSVVASDAFFP
FADGMLACIKAGATAVIQPGGSMRDEEVIKAADEHGIAMVFTGVRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=473, Percent_Identity=39.7463002114165, Blast_Score=280, Evalue=2e-75,
Organism=Escherichia coli, GI1790439, Length=538, Percent_Identity=56.5055762081784, Blast_Score=561, Evalue=1e-161,
Organism=Caenorhabditis elegans, GI71985564, Length=475, Percent_Identity=36.2105263157895, Blast_Score=251, Evalue=9e-67,
Organism=Caenorhabditis elegans, GI71985574, Length=305, Percent_Identity=27.8688524590164, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6323056, Length=602, Percent_Identity=33.2225913621262, Blast_Score=289, Evalue=8e-79,
Organism=Saccharomyces cerevisiae, GI6323768, Length=474, Percent_Identity=37.5527426160338, Blast_Score=272, Evalue=1e-73,
Organism=Drosophila melanogaster, GI24649832, Length=600, Percent_Identity=36.6666666666667, Blast_Score=308, Evalue=8e-84,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 56465; Mature: 56334

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDRPRRVTRALLSVSDKTGLTEFARALADLGVELVSTGGTAKEIAAAGLKVSDVSDLTG
CCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHEECCCCCHHHHHHCCCEECCCHHHCC
FPEMMDGRVKTLHPKVHGGLLAIRDNADHAKSMKDHGIAPIDLLVVNLYPFESTVDKGAA
CHHHHCCCEEEECCHHCCCEEEEECCCHHHHHHHHCCCCCEEEEEEEEECCHHHHCCCCH
CEECIENIDIGGPAMIRAAAKNHDDVAVVVEPQDYQAVLDELKANAGATTLSLRKRLAAK
HHHHHHHCCCCCHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHH
AYARTAAYDAAISNWFAVQLETDAPDYRAVGGRLAQRLRYGENPHQTAAFYRTPERRAGV
HHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHEEECCHHHHHH
ATARQLQGKELSYNNINDTDAAYECVAEFDAARTAACVIIKHANPCGVAEGSSLTEAYRR
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCHHHHHHHH
ALACDQTSAYGGIIAFNRTIDADAANAVAGIFTEVIIAPDATEEAIAVIGKRKNLRLLLA
HHHCCCCCCCCCEEEECCEECCCHHHHHHHHHHHHEECCCCCHHHHHHHCCCCCEEEEEE
GGLPDPRARGLTAKTVAGGLLVQGRDNAVIDDMSLKVVTKRPPTEAEMRDLRFAFRVAKH
CCCCCCHHCCCCHHHHCCCEEEECCCCCEEECCEEEEEECCCCCHHHHHHHHHHHHHHHH
VKSNTIVYARDLATVGIGAGQMSRVDSARIAARKAEDAARDLKLAEPLTKGSVVASDAFF
HCCCEEEEEECHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCC
PFADGMLACIKAGATAVIQPGGSMRDEEVIKAADEHGIAMVFTGVRHFRH
CHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHCCCCEEEEEEHHHHHCC
>Mature Secondary Structure 
TDRPRRVTRALLSVSDKTGLTEFARALADLGVELVSTGGTAKEIAAAGLKVSDVSDLTG
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHEECCCCCHHHHHHCCCEECCCHHHCC
FPEMMDGRVKTLHPKVHGGLLAIRDNADHAKSMKDHGIAPIDLLVVNLYPFESTVDKGAA
CHHHHCCCEEEECCHHCCCEEEEECCCHHHHHHHHCCCCCEEEEEEEEECCHHHHCCCCH
CEECIENIDIGGPAMIRAAAKNHDDVAVVVEPQDYQAVLDELKANAGATTLSLRKRLAAK
HHHHHHHCCCCCHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHH
AYARTAAYDAAISNWFAVQLETDAPDYRAVGGRLAQRLRYGENPHQTAAFYRTPERRAGV
HHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHEEECCHHHHHH
ATARQLQGKELSYNNINDTDAAYECVAEFDAARTAACVIIKHANPCGVAEGSSLTEAYRR
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCHHHHHHHH
ALACDQTSAYGGIIAFNRTIDADAANAVAGIFTEVIIAPDATEEAIAVIGKRKNLRLLLA
HHHCCCCCCCCCEEEECCEECCCHHHHHHHHHHHHEECCCCCHHHHHHHCCCCCEEEEEE
GGLPDPRARGLTAKTVAGGLLVQGRDNAVIDDMSLKVVTKRPPTEAEMRDLRFAFRVAKH
CCCCCCHHCCCCHHHHCCCEEEECCCCCEEECCEEEEEECCCCCHHHHHHHHHHHHHHHH
VKSNTIVYARDLATVGIGAGQMSRVDSARIAARKAEDAARDLKLAEPLTKGSVVASDAFF
HCCCEEEEEECHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCC
PFADGMLACIKAGATAVIQPGGSMRDEEVIKAADEHGIAMVFTGVRHFRH
CHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHCCCCEEEEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]