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The map label for this gene is xthA [H]

Identifier: 75674284

GI number: 75674284

Start: 102283

End: 103170

Strand: Direct

Name: xthA [H]

Synonym: Nwi_0085

Alternate gene names: 75674284

Gene position: 102283-103170 (Clockwise)

Preceding gene: 75674283

Following gene: 75674287

Centisome position: 3.01

GC content: 63.4

Gene sequence:

>888_bases
TTGTGGGAGAGGGTGCCCGAGCATCCGCGAGGGCGGGTGAAGGTAAGAACCATCGACCGTACCAAGCACCCCATGCACCT
CACCTTGACCACCTGGAATATCAACTCGGTGCGGCTGCGCATCGATCTGGTCGCCAGGTTTCTGAAAGCGCAGCGGCCGG
ACGTTCTGTGCCTGCAGGAGACCAAATGCCCCGATGACGCGTTTCCGCTGAAACGCTTTCACCGGCTCGGCTATGAGCAC
ATCGCGCTCAACGGCCAGAAGGGATATCACGGGGTGGCGGTGGTCTCGAAGCTGCCTTTCGACGCCAGCAACATCCGCAC
ATTCTGCGACAAGATCGACTCCCGCCACGTCAGCGTGATCCTCGGCGGCGAGGCGGGTCTCGCCGCGCCGCTGGTGCTGC
ATAACTTCTACGTGCCCGCGGGCGGCGACATTCCCGATCCCGCGCTCAATCCGAAGTTCGCCCACAAGCTGCAGTTTCTC
GACGAGATGAAAGGCTGCGCGCCGCTGCATCCGGACAACGGCGACCGGCATGTGCTGGTCGGCGATCTCAACGTCGCTCC
GCATGAGAACGACGTCTGGTCGCACAAACAGCTCCTCAAGATCGTCTCGCACACACCCATCGAATGCGAAAAGCTGCTCG
GCGTGCAGGATCGCGGCAACTGGGTCGACATCGCGCGCGCGCGGATCCCGATGTCGGAGAAAGTCTATACGTGGTGGAGC
TATCGCGCCGCCGACTGGGTCGCCGCCAATCGCGGCCGCAGGCTCGATCATATCTGGGTCTCGGAGGCGCTGCGCGACGG
AATCCGCGACTTCCGCATCACTCGCGACGCCCGCGGCTGGGAGCGTCCATCCGATCATGTCCCGGTGACAGCGGTGATCG
AGGTGTGA

Upstream 100 bases:

>100_bases
ACGGCGAAGCTCGATCCCGCGCTGTTCAGAATCAACGGCACCATGCATCCGGCATCGACCAACAATTGAGCGCTGAAGTC
CTCTTCCTTTCCCTCTCGCC

Downstream 100 bases:

>100_bases
GCACCTTTGCCGCGGTGGGGTGAGCAACCCGTGTGACGGCAACGCCGCTATAGTCTTTTCGCTTCCGACGAAATCGGAAG
CGAGGCTCTGAAAAACGCGA

Product: exodeoxyribonuclease III xth

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MWERVPEHPRGRVKVRTIDRTKHPMHLTLTTWNINSVRLRIDLVARFLKAQRPDVLCLQETKCPDDAFPLKRFHRLGYEH
IALNGQKGYHGVAVVSKLPFDASNIRTFCDKIDSRHVSVILGGEAGLAAPLVLHNFYVPAGGDIPDPALNPKFAHKLQFL
DEMKGCAPLHPDNGDRHVLVGDLNVAPHENDVWSHKQLLKIVSHTPIECEKLLGVQDRGNWVDIARARIPMSEKVYTWWS
YRAADWVAANRGRRLDHIWVSEALRDGIRDFRITRDARGWERPSDHVPVTAVIEV

Sequences:

>Translated_295_residues
MWERVPEHPRGRVKVRTIDRTKHPMHLTLTTWNINSVRLRIDLVARFLKAQRPDVLCLQETKCPDDAFPLKRFHRLGYEH
IALNGQKGYHGVAVVSKLPFDASNIRTFCDKIDSRHVSVILGGEAGLAAPLVLHNFYVPAGGDIPDPALNPKFAHKLQFL
DEMKGCAPLHPDNGDRHVLVGDLNVAPHENDVWSHKQLLKIVSHTPIECEKLLGVQDRGNWVDIARARIPMSEKVYTWWS
YRAADWVAANRGRRLDHIWVSEALRDGIRDFRITRDARGWERPSDHVPVTAVIEV
>Mature_295_residues
MWERVPEHPRGRVKVRTIDRTKHPMHLTLTTWNINSVRLRIDLVARFLKAQRPDVLCLQETKCPDDAFPLKRFHRLGYEH
IALNGQKGYHGVAVVSKLPFDASNIRTFCDKIDSRHVSVILGGEAGLAAPLVLHNFYVPAGGDIPDPALNPKFAHKLQFL
DEMKGCAPLHPDNGDRHVLVGDLNVAPHENDVWSHKQLLKIVSHTPIECEKLLGVQDRGNWVDIARARIPMSEKVYTWWS
YRAADWVAANRGRRLDHIWVSEALRDGIRDFRITRDARGWERPSDHVPVTAVIEV

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Escherichia coli, GI1788046, Length=273, Percent_Identity=29.3040293040293, Blast_Score=105, Evalue=4e-24,
Organism=Drosophila melanogaster, GI221330655, Length=279, Percent_Identity=29.0322580645161, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI17136678, Length=279, Percent_Identity=29.0322580645161, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 33678; Mature: 33678

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWERVPEHPRGRVKVRTIDRTKHPMHLTLTTWNINSVRLRIDLVARFLKAQRPDVLCLQE
CCCCCCCCCCCCEEEEEECCCCCCEEEEEEEEECCEEEEEHHHHHHHHHCCCCCEEEEEC
TKCPDDAFPLKRFHRLGYEHIALNGQKGYHGVAVVSKLPFDASNIRTFCDKIDSRHVSVI
CCCCCCCHHHHHHHHCCCEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEE
LGGEAGLAAPLVLHNFYVPAGGDIPDPALNPKFAHKLQFLDEMKGCAPLHPDNGDRHVLV
ECCCCCCHHHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
GDLNVAPHENDVWSHKQLLKIVSHTPIECEKLLGVQDRGNWVDIARARIPMSEKVYTWWS
EECCCCCCCCCHHHHHHHHHHHHCCCCCHHHHCCCCCCCCEEEEHHHCCCCCCCEEEEEE
YRAADWVAANRGRRLDHIWVSEALRDGIRDFRITRDARGWERPSDHVPVTAVIEV
CCCHHHHHHCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCEEEEEEC
>Mature Secondary Structure
MWERVPEHPRGRVKVRTIDRTKHPMHLTLTTWNINSVRLRIDLVARFLKAQRPDVLCLQE
CCCCCCCCCCCCEEEEEECCCCCCEEEEEEEEECCEEEEEHHHHHHHHHCCCCCEEEEEC
TKCPDDAFPLKRFHRLGYEHIALNGQKGYHGVAVVSKLPFDASNIRTFCDKIDSRHVSVI
CCCCCCCHHHHHHHHCCCEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEE
LGGEAGLAAPLVLHNFYVPAGGDIPDPALNPKFAHKLQFLDEMKGCAPLHPDNGDRHVLV
ECCCCCCHHHHHHHEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
GDLNVAPHENDVWSHKQLLKIVSHTPIECEKLLGVQDRGNWVDIARARIPMSEKVYTWWS
EECCCCCCCCCHHHHHHHHHHHHCCCCCHHHHCCCCCCCCEEEEHHHCCCCCCCEEEEEE
YRAADWVAANRGRRLDHIWVSEALRDGIRDFRITRDARGWERPSDHVPVTAVIEV
CCCHHHHHHCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]