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The map label for this gene is truB

Identifier: 75674225

GI number: 75674225

Start: 28031

End: 29107

Strand: Direct

Name: truB

Synonym: Nwi_0026

Alternate gene names: 75674225

Gene position: 28031-29107 (Clockwise)

Preceding gene: 75674224

Following gene: 75674226

Centisome position: 0.82

GC content: 66.3

Gene sequence:

>1077_bases
ATGACCACGCCTGACGCCGCCATCGACTCCAAAATCAGCGATTCGAACGGCGCCGACAAAAATAAATCCGCGGCCGACGA
TAACGCCTTCAACGCGCCGGGCCGGAAAAGACACCACAACAACCAGCCGCGCCGCGACAAGCGTGACGTCCATGGCTGGG
TTGCGCTCGACAAGCCGGTCGGCATGACCTCGACCCAGGCGGTCGCGGTGGTGAAGCGGCTGTTCTCCGCCAAGCGCGCC
GGCCACGCCGGGACGCTCGATCCGCTCGCCTCCGGCGGCCTTCCGATCGCGCTCGGCGAGGCCACCAAGACGGTGCCGTT
CGTGATGGACGGCCGCAAGCGTTACCGCTTCACGGTGGCCTGGGGCGAGGAACGCGACACCGACGACACCGAGGGCCGCG
CGGTGGCGACCAGCGCCGAGCGCCCCACGGCGGAGGCGATCATGGCGCTGCTGCCGAGCTTCACCGGCAAGATCGAGCAA
ATCCCGCCGCAATATTCGGCAGTGAAGATCCAGGGTGAGCGCGCCTACGATCTGGCGCGCGACGGCGAGGTCGTGCCGCT
GGCGCCCCGCCCGGTCGAGATACACCATTTAAATCTTGCCGATCATCAGGATAGCGGACATTCGGTGTTCGAGGCCGAAT
GCGGCAAGGGAACCTATGTCCGAGCGCTCGCCCGCGACATGGGGCGGCTGCTGGGCTGCTACGGCCATATCTGCGCGCTG
CGCCGGACGCTGGTCGGCCCGTTCGACGAGACGAGCATGATTCCGCTGGAACATCTGCAGGCTTTGTGCGATAGAGCCGC
GTCTGGCGAGGGAAACCTCGCCGACGCGCTTTTGCCCGTTGAGACCGCGCTGGACGACATCCCGGCACTGGCCGTCACAC
GGGCGGATGCGGCAAGGCTCCATCGGGGCCAAGCCGTTTTGTTGCGCGGACGGGATGCGCCCAATATGAGCGGCACGGTT
TATGTCACGGTGGCGGGCCGGCTTCTGGCCCTTGCCGAGCTTGGCAATGGCGAACTCATCCCCAAGCGCGTGTTCAACCT
CTCCGGACTGACCGCCAGTCCCCATCGCCAAGGATAG

Upstream 100 bases:

>100_bases
CGACGAGCGATTCGACGAAGCGGAACGTATCGAGAAGTTATTGAGAACGCCTGCGGTACAGAGAGATCTCGCACCGGATA
CCGACGAAAGCTGACCGATC

Downstream 100 bases:

>100_bases
TTTACGATGTCGATTGCCGCAGAACGCAAAGCGGAAGTCATCAAGACCAGCGCCAACAAGCCCGGCGATACTGGTTCGCC
CGAGGTTCAGGTCGCGATCC

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 358; Mature: 357

Protein sequence:

>358_residues
MTTPDAAIDSKISDSNGADKNKSAADDNAFNAPGRKRHHNNQPRRDKRDVHGWVALDKPVGMTSTQAVAVVKRLFSAKRA
GHAGTLDPLASGGLPIALGEATKTVPFVMDGRKRYRFTVAWGEERDTDDTEGRAVATSAERPTAEAIMALLPSFTGKIEQ
IPPQYSAVKIQGERAYDLARDGEVVPLAPRPVEIHHLNLADHQDSGHSVFEAECGKGTYVRALARDMGRLLGCYGHICAL
RRTLVGPFDETSMIPLEHLQALCDRAASGEGNLADALLPVETALDDIPALAVTRADAARLHRGQAVLLRGRDAPNMSGTV
YVTVAGRLLALAELGNGELIPKRVFNLSGLTASPHRQG

Sequences:

>Translated_358_residues
MTTPDAAIDSKISDSNGADKNKSAADDNAFNAPGRKRHHNNQPRRDKRDVHGWVALDKPVGMTSTQAVAVVKRLFSAKRA
GHAGTLDPLASGGLPIALGEATKTVPFVMDGRKRYRFTVAWGEERDTDDTEGRAVATSAERPTAEAIMALLPSFTGKIEQ
IPPQYSAVKIQGERAYDLARDGEVVPLAPRPVEIHHLNLADHQDSGHSVFEAECGKGTYVRALARDMGRLLGCYGHICAL
RRTLVGPFDETSMIPLEHLQALCDRAASGEGNLADALLPVETALDDIPALAVTRADAARLHRGQAVLLRGRDAPNMSGTV
YVTVAGRLLALAELGNGELIPKRVFNLSGLTASPHRQG
>Mature_357_residues
TTPDAAIDSKISDSNGADKNKSAADDNAFNAPGRKRHHNNQPRRDKRDVHGWVALDKPVGMTSTQAVAVVKRLFSAKRAG
HAGTLDPLASGGLPIALGEATKTVPFVMDGRKRYRFTVAWGEERDTDDTEGRAVATSAERPTAEAIMALLPSFTGKIEQI
PPQYSAVKIQGERAYDLARDGEVVPLAPRPVEIHHLNLADHQDSGHSVFEAECGKGTYVRALARDMGRLLGCYGHICALR
RTLVGPFDETSMIPLEHLQALCDRAASGEGNLADALLPVETALDDIPALAVTRADAARLHRGQAVLLRGRDAPNMSGTVY
VTVAGRLLALAELGNGELIPKRVFNLSGLTASPHRQG

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=257, Percent_Identity=34.6303501945525, Blast_Score=119, Evalue=6e-27,
Organism=Escherichia coli, GI2367200, Length=315, Percent_Identity=39.6825396825397, Blast_Score=208, Evalue=5e-55,
Organism=Caenorhabditis elegans, GI17553978, Length=274, Percent_Identity=27.7372262773723, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323204, Length=248, Percent_Identity=29.8387096774194, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324037, Length=131, Percent_Identity=33.587786259542, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281364189, Length=215, Percent_Identity=26.5116279069767, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281364187, Length=215, Percent_Identity=26.5116279069767, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281364185, Length=215, Percent_Identity=26.5116279069767, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281364183, Length=215, Percent_Identity=26.5116279069767, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI62471759, Length=215, Percent_Identity=26.5116279069767, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17975520, Length=215, Percent_Identity=26.5116279069767, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_NITWN (Q3SWP7)

Other databases:

- EMBL:   CP000115
- RefSeq:   YP_316646.1
- ProteinModelPortal:   Q3SWP7
- SMR:   Q3SWP7
- STRING:   Q3SWP7
- GeneID:   3675149
- GenomeReviews:   CP000115_GR
- KEGG:   nwi:Nwi_0026
- NMPDR:   fig|323098.3.peg.42
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   LGCGAYV
- PhylomeDB:   Q3SWP7
- ProtClustDB:   PRK05389
- BioCyc:   NWIN323098:NWI_0026-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom; SSF88697 PUA-like

EC number: 4.2.1.70

Molecular weight: Translated: 38378; Mature: 38247

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: NA

Important sites: ACT_SITE 87-87

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPDAAIDSKISDSNGADKNKSAADDNAFNAPGRKRHHNNQPRRDKRDVHGWVALDKPV
CCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCHHHCCCEEEEECCCC
GMTSTQAVAVVKRLFSAKRAGHAGTLDPLASGGLPIALGEATKTVPFVMDGRKRYRFTVA
CCCHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCEEEECCCCCCCCEEECCCEEEEEEEE
WGEERDTDDTEGRAVATSAERPTAEAIMALLPSFTGKIEQIPPQYSAVKIQGERAYDLAR
ECCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCHHHCCCCCEEEEEECCCCCHHCC
DGEVVPLAPRPVEIHHLNLADHQDSGHSVFEAECGKGTYVRALARDMGRLLGCYGHICAL
CCCEEECCCCCEEEEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
RRTLVGPFDETSMIPLEHLQALCDRAASGEGNLADALLPVETALDDIPALAVTRADAARL
HHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHEEEHHHHHHH
HRGQAVLLRGRDAPNMSGTVYVTVAGRLLALAELGNGELIPKRVFNLSGLTASPHRQG
CCCCEEEEECCCCCCCCCEEEEEECCHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TTPDAAIDSKISDSNGADKNKSAADDNAFNAPGRKRHHNNQPRRDKRDVHGWVALDKPV
CCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCHHHCCCEEEEECCCC
GMTSTQAVAVVKRLFSAKRAGHAGTLDPLASGGLPIALGEATKTVPFVMDGRKRYRFTVA
CCCHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCEEEECCCCCCCCEEECCCEEEEEEEE
WGEERDTDDTEGRAVATSAERPTAEAIMALLPSFTGKIEQIPPQYSAVKIQGERAYDLAR
ECCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCHHHCCCCCEEEEEECCCCCHHCC
DGEVVPLAPRPVEIHHLNLADHQDSGHSVFEAECGKGTYVRALARDMGRLLGCYGHICAL
CCCEEECCCCCEEEEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
RRTLVGPFDETSMIPLEHLQALCDRAASGEGNLADALLPVETALDDIPALAVTRADAARL
HHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHEEEHHHHHHH
HRGQAVLLRGRDAPNMSGTVYVTVAGRLLALAELGNGELIPKRVFNLSGLTASPHRQG
CCCCEEEEECCCCCCCCCEEEEEECCHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA