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The map label for this gene is miaB

Identifier: 75674213

GI number: 75674213

Start: 13929

End: 15362

Strand: Direct

Name: miaB

Synonym: Nwi_0014

Alternate gene names: 75674213

Gene position: 13929-15362 (Clockwise)

Preceding gene: 75674212

Following gene: 75674214

Centisome position: 0.41

GC content: 65.83

Gene sequence:

>1434_bases
ATGCCGGAAAGCCGCATGCCGCCGCGCAAGCTGCACATCAAATCCTACGGTTGCCAGATGAACGTCTACGACGCCCAGCG
CATGGCGGACACGCTGGCGGTCGAAGGATATGTCGAGACCACCAGCGCCGAGGACGCCGACCTCGTCATTCTCAACACCT
GCCACATTCGCGAGAAGGCGTCCGAGAAGGTCTATTCCGAGCTCGGCCGCCTGCGCGCAGCCAAGGATCAGGCGGCGCGT
GATGGCCGCGGCATGAACATCGTGGTCGCCGGCTGCGTCGCGCAGGCCGAAGGCGAGGAGATCATCAGGCGCGCGCCCAT
GGTCGATGTGGTGGTGGGTCCGCAGAGCTATCATCATCTGCCGCAACTGCTCGCGCGCGCAAAGACCTGTGGCCGTGCGC
TGGAGACCGAGTTTCCGGTCGCGGACAAGTTCGGCTTCCTGCCGCAGCCGTCGCGGCAGGCGATCCGTTCCCGTGGCATC
TCGGCGTTCGTGACGGTGCAGGAAGGCTGCGACAAATTCTGCACCTTCTGCGTCGTGCCTTATACCCGCGGCGCCGAGGC
GTCGCGGCCGGTGGCGAAGGTCGTCGAAGACGTGCGGCGCCTGGCGGACAACGGCGTTCGCGAGATCACCCTCATCGGAC
AGAACGTCAACGCCTATCACGGCGAGGGTCCCGACGGGCGGCCCTGGCCGTTCGGCAAGCTGCTGTATCGTCTCGCCGGG
ATTCCAGGCATCGTCCGGCTGCGTTACTCGACCAGCCATCCGCGCGACGTCGAAGACACCCTGATCGACGCCCACCGCGA
TCTCGACGCGCTGATGCCGTTCGTGCACCTGCCGGTGCAATCCGGATCAGACCGCATCCTCGCCGCCATGAATCGCAGGC
ATACCGCCGATGATTACCGCCGGGTGATCGACCGGTTTCGTCAGGCCCGGCCCGACATCGCGTTCTCCTCGGACTTCATC
GTCGGCTTCCCCGGCGAAACCGAGGAAGATTTCGCCGCCACCCTCGCGCTCGTCGGCCAAATCGGATACGCTGCCGCCTA
TTCGTTCAAATACTCGCCCCGACCCGGAACGCCGGCGGCGGACATGCAGGAGACGGTGTCAACGGCCGACATGGACCAGC
GATTGGTGCAGCTTCAGAACCTGATCGACAGCCAGCAATCCGCCTTCAACCGGACCGCGCTGGGCAGGACGATCGACGTG
CTGTTCGATCGCGCCGGACGCAAGCCCGGCCAGATCGTCGGCCGCACGGCCTATCTTCAGCCCGCCCATGTGGAAGCGTC
GGACGCCATCATCGGACAGGTGCTGCCCGTAACCGTCGCCAGTCTGGAACGCTACAGCCTGTTCGGAACGCTGGCGTCCA
AGCCGACGTCCGGCGAACCTTCGAACCATGCCGCGACGGGCGGCGCGCAGTTCCAGACGACCGCCGGAGCCTGA

Upstream 100 bases:

>100_bases
GCCGAGGTCGCGCGCAAGCTCGGCTACCGGCTGGTCGATCATCGGCTCGAACTCTATTGCGTGCCGATGGATGACGAATA
AGTCGCGGGTGCCTGTCGAA

Downstream 100 bases:

>100_bases
CCCCTTGCCCAAGAGCGCATCGGATTCCCCTCCGCTTGCTCCCAGCCGGAAACCCGAGATCCAGGGCCCGCCCGAAACGC
AGGTCGTCATCGATTTCGAG

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MPESRMPPRKLHIKSYGCQMNVYDAQRMADTLAVEGYVETTSAEDADLVILNTCHIREKASEKVYSELGRLRAAKDQAAR
DGRGMNIVVAGCVAQAEGEEIIRRAPMVDVVVGPQSYHHLPQLLARAKTCGRALETEFPVADKFGFLPQPSRQAIRSRGI
SAFVTVQEGCDKFCTFCVVPYTRGAEASRPVAKVVEDVRRLADNGVREITLIGQNVNAYHGEGPDGRPWPFGKLLYRLAG
IPGIVRLRYSTSHPRDVEDTLIDAHRDLDALMPFVHLPVQSGSDRILAAMNRRHTADDYRRVIDRFRQARPDIAFSSDFI
VGFPGETEEDFAATLALVGQIGYAAAYSFKYSPRPGTPAADMQETVSTADMDQRLVQLQNLIDSQQSAFNRTALGRTIDV
LFDRAGRKPGQIVGRTAYLQPAHVEASDAIIGQVLPVTVASLERYSLFGTLASKPTSGEPSNHAATGGAQFQTTAGA

Sequences:

>Translated_477_residues
MPESRMPPRKLHIKSYGCQMNVYDAQRMADTLAVEGYVETTSAEDADLVILNTCHIREKASEKVYSELGRLRAAKDQAAR
DGRGMNIVVAGCVAQAEGEEIIRRAPMVDVVVGPQSYHHLPQLLARAKTCGRALETEFPVADKFGFLPQPSRQAIRSRGI
SAFVTVQEGCDKFCTFCVVPYTRGAEASRPVAKVVEDVRRLADNGVREITLIGQNVNAYHGEGPDGRPWPFGKLLYRLAG
IPGIVRLRYSTSHPRDVEDTLIDAHRDLDALMPFVHLPVQSGSDRILAAMNRRHTADDYRRVIDRFRQARPDIAFSSDFI
VGFPGETEEDFAATLALVGQIGYAAAYSFKYSPRPGTPAADMQETVSTADMDQRLVQLQNLIDSQQSAFNRTALGRTIDV
LFDRAGRKPGQIVGRTAYLQPAHVEASDAIIGQVLPVTVASLERYSLFGTLASKPTSGEPSNHAATGGAQFQTTAGA
>Mature_476_residues
PESRMPPRKLHIKSYGCQMNVYDAQRMADTLAVEGYVETTSAEDADLVILNTCHIREKASEKVYSELGRLRAAKDQAARD
GRGMNIVVAGCVAQAEGEEIIRRAPMVDVVVGPQSYHHLPQLLARAKTCGRALETEFPVADKFGFLPQPSRQAIRSRGIS
AFVTVQEGCDKFCTFCVVPYTRGAEASRPVAKVVEDVRRLADNGVREITLIGQNVNAYHGEGPDGRPWPFGKLLYRLAGI
PGIVRLRYSTSHPRDVEDTLIDAHRDLDALMPFVHLPVQSGSDRILAAMNRRHTADDYRRVIDRFRQARPDIAFSSDFIV
GFPGETEEDFAATLALVGQIGYAAAYSFKYSPRPGTPAADMQETVSTADMDQRLVQLQNLIDSQQSAFNRTALGRTIDVL
FDRAGRKPGQIVGRTAYLQPAHVEASDAIIGQVLPVTVASLERYSLFGTLASKPTSGEPSNHAATGGAQFQTTAGA

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=433, Percent_Identity=33.9491916859122, Blast_Score=268, Evalue=9e-72,
Organism=Homo sapiens, GI28872782, Length=433, Percent_Identity=33.9491916859122, Blast_Score=268, Evalue=9e-72,
Organism=Homo sapiens, GI93277076, Length=468, Percent_Identity=28.8461538461538, Blast_Score=176, Evalue=4e-44,
Organism=Escherichia coli, GI1786882, Length=447, Percent_Identity=46.3087248322148, Blast_Score=401, Evalue=1e-113,
Organism=Escherichia coli, GI1787057, Length=473, Percent_Identity=28.9640591966173, Blast_Score=172, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI17553146, Length=423, Percent_Identity=32.387706855792, Blast_Score=236, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI71996771, Length=386, Percent_Identity=26.4248704663212, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI21356207, Length=431, Percent_Identity=32.9466357308585, Blast_Score=258, Evalue=5e-69,
Organism=Drosophila melanogaster, GI19922432, Length=466, Percent_Identity=29.1845493562232, Blast_Score=173, Evalue=3e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_NITWN (Q3SMT7)

Other databases:

- EMBL:   CP000115
- RefSeq:   YP_316634.1
- ProteinModelPortal:   Q3SMT7
- SMR:   Q3SMT7
- STRING:   Q3SMT7
- GeneID:   3676454
- GenomeReviews:   CP000115_GR
- KEGG:   nwi:Nwi_0014
- NMPDR:   fig|323098.3.peg.11
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   SYHHLPQ
- PhylomeDB:   Q3SMT7
- ProtClustDB:   PRK14325
- BioCyc:   NWIN323098:NWI_0014-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 52191; Mature: 52059

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPESRMPPRKLHIKSYGCQMNVYDAQRMADTLAVEGYVETTSAEDADLVILNTCHIREKA
CCCCCCCCCEEEEEECCCEEECHHHHHHHHHHHHCCEEECCCCCCCCEEEEEECHHHHHH
SEKVYSELGRLRAAKDQAARDGRGMNIVVAGCVAQAEGEEIIRRAPMVDVVVGPQSYHHL
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHCCHHHHHHHCCCEEEEECCHHHHHH
PQLLARAKTCGRALETEFPVADKFGFLPQPSRQAIRSRGISAFVTVQEGCDKFCTFCVVP
HHHHHHHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHCCCEEEEEHHHHHHHHHHEEECC
YTRGAEASRPVAKVVEDVRRLADNGVREITLIGQNVNAYHGEGPDGRPWPFGKLLYRLAG
CCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHC
IPGIVRLRYSTSHPRDVEDTLIDAHRDLDALMPFVHLPVQSGSDRILAAMNRRHTADDYR
CCEEEEEEECCCCCCCHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHH
RVIDRFRQARPDIAFSSDFIVGFPGETEEDFAATLALVGQIGYAAAYSFKYSPRPGTPAA
HHHHHHHHCCCCCEECCCEEEECCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCHH
DMQETVSTADMDQRLVQLQNLIDSQQSAFNRTALGRTIDVLFDRAGRKPGQIVGRTAYLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCEEECC
PAHVEASDAIIGQVLPVTVASLERYSLFGTLASKPTSGEPSNHAATGGAQFQTTAGA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCC
>Mature Secondary Structure 
PESRMPPRKLHIKSYGCQMNVYDAQRMADTLAVEGYVETTSAEDADLVILNTCHIREKA
CCCCCCCCEEEEEECCCEEECHHHHHHHHHHHHCCEEECCCCCCCCEEEEEECHHHHHH
SEKVYSELGRLRAAKDQAARDGRGMNIVVAGCVAQAEGEEIIRRAPMVDVVVGPQSYHHL
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHCCHHHHHHHCCCEEEEECCHHHHHH
PQLLARAKTCGRALETEFPVADKFGFLPQPSRQAIRSRGISAFVTVQEGCDKFCTFCVVP
HHHHHHHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHCCCEEEEEHHHHHHHHHHEEECC
YTRGAEASRPVAKVVEDVRRLADNGVREITLIGQNVNAYHGEGPDGRPWPFGKLLYRLAG
CCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHC
IPGIVRLRYSTSHPRDVEDTLIDAHRDLDALMPFVHLPVQSGSDRILAAMNRRHTADDYR
CCEEEEEEECCCCCCCHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHH
RVIDRFRQARPDIAFSSDFIVGFPGETEEDFAATLALVGQIGYAAAYSFKYSPRPGTPAA
HHHHHHHHCCCCCEECCCEEEECCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCHH
DMQETVSTADMDQRLVQLQNLIDSQQSAFNRTALGRTIDVLFDRAGRKPGQIVGRTAYLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCEEECC
PAHVEASDAIIGQVLPVTVASLERYSLFGTLASKPTSGEPSNHAATGGAQFQTTAGA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA