Click here to switch to the map view.

The map label for this gene is gltD [H]

Identifier: 73542809

GI number: 73542809

Start: 3430360

End: 3431823

Strand: Reverse

Name: gltD [H]

Synonym: Reut_A3125

Alternate gene names: 73542809

Gene position: 3431823-3430360 (Counterclockwise)

Preceding gene: 73542810

Following gene: 73542807

Centisome position: 90.16

GC content: 63.73

Gene sequence:

>1464_bases
ATGGGTAAGGCGACTGGCTTTCTCGAATTTCCGCGCCAGAACGAAGGCTACGAAGCGGTAGTCAAGCGCGTGAAGCACTA
CAAGGAATTCGTGTTTGCGCTGTCCGACAGCGAGGCGAAGATCCAGGGTGCGCGCTGCATGGACTGCGGCATCCCGTTCT
GCAACAACGGCTGTCCGGTCAACAACATCATTCCCGACTTCAATGACCTGGTGTACCGCCAGGACTGGAAGTCGGCGATC
GAAGTGCTGCACCAGACCAACAACTTCCCCGAGTTCACGGGCCGCATCTGCCCGGCACCGTGCGAAGCTGCCTGCACGCT
CGGCATCAATGAGCTGCCGGTCGGCATCAAGTCGATCGAGCACGCGATCATCGACAAGGCCTGGGAAGAAGGCTGGGTCG
CACCGCAGCCGCCGAAGCACAAGACCGGCAAGACCGTCGCCGTGGTCGGCTCGGGCCCCGCCGGCATGGCGGCCGCGCAG
CAACTGGCACGCGCCGGCCATGACGTGACCGTGTTCGAAAAGAACGATCGCATCGGCGGCCTGCTGCGCTACGGCATCCC
CGACTTCAAGATGGAGAAGACGCTGATCGACCGCCGCATCGAGCAGATGCAGGCCGAAGGCGTGACTTTCCGCGCAGGCG
TGATGGTGACCGACGGCGCGCTGCCGGCCGGCATCAAGAACTATGCGCGTGAAACCATCTCCGCCAAGGATCTGATGGAC
CAGTTCGACGCTGTCGTGCTGGCCGGCGGTTCGGAAGTGCCGCGCGACCTGCCGGTGCCGGGCCGTGACCTGGATGGCAT
CCACTTCGCGCTGGAATTCCTGATTCCGCAGAACAAGGAAGTGGCCGGCGACGGCGAGAACGAGATCCGTGCCGAAGGCA
AGAACGTGATCGTGATCGGCGGTGGCGATACGGGTTCCGACTGCGTCGGTACCTCGAACCGCCATGGCGCGACCTCGGTG
AACCAGTTCGAACTGCTGCCGCAGCCGCCGGAAGAAGAGGACAAGCCGCTGGTGTGGCCGTACTGGCCGATCAAGCTGCG
CACCTCGTCGTCGCATGAAGAAGGTTGCGATCGCGACTGGTCCGTCGCCACCAAGGAATTCATCGGCGAAAACGGCAAGG
TCACCGCGCTGAAGGCCTGCCGCGTCGAGTGGAAGGACGGCAAGATGCAGGAAGTGCCGGGCAGCGAGTTCATTCTGCCG
GCCGACCTCGTGCTGCTGGCGATGGGCTTTACCAATCCGGTGGGTTCGATGCTGGACGCATTCGGTGTTGACACCGATGC
GCGCAAGAACGCGAAGGCTGCCACGGAAGGCGAGCGTGCGTACCACACCAATGTGCCGAAGGTGTTCGCCGCTGGCGACG
TGCGCCGTGGCCAGTCGCTGGTGGTGTGGGCGATTCGGGAAGGCCGCCAGGCTGCGCGTTCGGTAGACGCGTTCCTGATG
GGGCATACGGAACTGCCGCGCTGA

Upstream 100 bases:

>100_bases
GATCGCCCCAACGGTAACACCGGCGACGATCCCGACAAGATACTGACCTCACAGCAAGGCCGGCCACGCCGGTCTTGCCT
AGCAAGATCAAGGACGCAAC

Downstream 100 bases:

>100_bases
TCAGCTGGAAAGCTGTTTCAGGAAAGACGCCGGGTGAATCCCGGCGTTTTTTTGCCTCGATAAGACGCTCAGGCAGCGAG
CACACGCTCCATCATCATCG

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MGKATGFLEFPRQNEGYEAVVKRVKHYKEFVFALSDSEAKIQGARCMDCGIPFCNNGCPVNNIIPDFNDLVYRQDWKSAI
EVLHQTNNFPEFTGRICPAPCEAACTLGINELPVGIKSIEHAIIDKAWEEGWVAPQPPKHKTGKTVAVVGSGPAGMAAAQ
QLARAGHDVTVFEKNDRIGGLLRYGIPDFKMEKTLIDRRIEQMQAEGVTFRAGVMVTDGALPAGIKNYARETISAKDLMD
QFDAVVLAGGSEVPRDLPVPGRDLDGIHFALEFLIPQNKEVAGDGENEIRAEGKNVIVIGGGDTGSDCVGTSNRHGATSV
NQFELLPQPPEEEDKPLVWPYWPIKLRTSSSHEEGCDRDWSVATKEFIGENGKVTALKACRVEWKDGKMQEVPGSEFILP
ADLVLLAMGFTNPVGSMLDAFGVDTDARKNAKAATEGERAYHTNVPKVFAAGDVRRGQSLVVWAIREGRQAARSVDAFLM
GHTELPR

Sequences:

>Translated_487_residues
MGKATGFLEFPRQNEGYEAVVKRVKHYKEFVFALSDSEAKIQGARCMDCGIPFCNNGCPVNNIIPDFNDLVYRQDWKSAI
EVLHQTNNFPEFTGRICPAPCEAACTLGINELPVGIKSIEHAIIDKAWEEGWVAPQPPKHKTGKTVAVVGSGPAGMAAAQ
QLARAGHDVTVFEKNDRIGGLLRYGIPDFKMEKTLIDRRIEQMQAEGVTFRAGVMVTDGALPAGIKNYARETISAKDLMD
QFDAVVLAGGSEVPRDLPVPGRDLDGIHFALEFLIPQNKEVAGDGENEIRAEGKNVIVIGGGDTGSDCVGTSNRHGATSV
NQFELLPQPPEEEDKPLVWPYWPIKLRTSSSHEEGCDRDWSVATKEFIGENGKVTALKACRVEWKDGKMQEVPGSEFILP
ADLVLLAMGFTNPVGSMLDAFGVDTDARKNAKAATEGERAYHTNVPKVFAAGDVRRGQSLVVWAIREGRQAARSVDAFLM
GHTELPR
>Mature_486_residues
GKATGFLEFPRQNEGYEAVVKRVKHYKEFVFALSDSEAKIQGARCMDCGIPFCNNGCPVNNIIPDFNDLVYRQDWKSAIE
VLHQTNNFPEFTGRICPAPCEAACTLGINELPVGIKSIEHAIIDKAWEEGWVAPQPPKHKTGKTVAVVGSGPAGMAAAQQ
LARAGHDVTVFEKNDRIGGLLRYGIPDFKMEKTLIDRRIEQMQAEGVTFRAGVMVTDGALPAGIKNYARETISAKDLMDQ
FDAVVLAGGSEVPRDLPVPGRDLDGIHFALEFLIPQNKEVAGDGENEIRAEGKNVIVIGGGDTGSDCVGTSNRHGATSVN
QFELLPQPPEEEDKPLVWPYWPIKLRTSSSHEEGCDRDWSVATKEFIGENGKVTALKACRVEWKDGKMQEVPGSEFILPA
DLVLLAMGFTNPVGSMLDAFGVDTDARKNAKAATEGERAYHTNVPKVFAAGDVRRGQSLVVWAIREGRQAARSVDAFLMG
HTELPR

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=466, Percent_Identity=23.6051502145923, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI1789606, Length=486, Percent_Identity=35.59670781893, Blast_Score=264, Evalue=8e-72,
Organism=Escherichia coli, GI1788811, Length=476, Percent_Identity=35.9243697478992, Blast_Score=255, Evalue=4e-69,
Organism=Escherichia coli, GI87082180, Length=471, Percent_Identity=33.3333333333333, Blast_Score=221, Evalue=6e-59,
Organism=Escherichia coli, GI1788468, Length=458, Percent_Identity=26.4192139737991, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1789243, Length=441, Percent_Identity=27.2108843537415, Blast_Score=114, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17570289, Length=509, Percent_Identity=44.4007858546169, Blast_Score=422, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6320030, Length=503, Percent_Identity=49.5029821073559, Blast_Score=469, Evalue=1e-133,
Organism=Drosophila melanogaster, GI28574881, Length=496, Percent_Identity=50.4032258064516, Blast_Score=491, Evalue=1e-139,
Organism=Drosophila melanogaster, GI24665539, Length=496, Percent_Identity=50.4032258064516, Blast_Score=491, Evalue=1e-139,
Organism=Drosophila melanogaster, GI24665547, Length=497, Percent_Identity=50.3018108651911, Blast_Score=489, Evalue=1e-138,
Organism=Drosophila melanogaster, GI24665543, Length=497, Percent_Identity=50.3018108651911, Blast_Score=489, Evalue=1e-138,
Organism=Drosophila melanogaster, GI24640763, Length=490, Percent_Identity=24.0816326530612, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI18858217, Length=490, Percent_Identity=24.0816326530612, Blast_Score=95, Evalue=1e-19,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 53145; Mature: 53014

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKATGFLEFPRQNEGYEAVVKRVKHYKEFVFALSDSEAKIQGARCMDCGIPFCNNGCPV
CCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCC
NNIIPDFNDLVYRQDWKSAIEVLHQTNNFPEFTGRICPAPCEAACTLGINELPVGIKSIE
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCHHHHCCCHHCCCCHHHHH
HAIIDKAWEEGWVAPQPPKHKTGKTVAVVGSGPAGMAAAQQLARAGHDVTVFEKNDRIGG
HHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCC
LLRYGIPDFKMEKTLIDRRIEQMQAEGVTFRAGVMVTDGALPAGIKNYARETISAKDLMD
EEECCCCCHHHHHHHHHHHHHHHHHCCEEEEECEEEECCCCCHHHHHHHHHHCCHHHHHH
QFDAVVLAGGSEVPRDLPVPGRDLDGIHFALEFLIPQNKEVAGDGENEIRAEGKNVIVIG
HHCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHCCCCEEEEEE
GGDTGSDCVGTSNRHGATSVNQFELLPQPPEEEDKPLVWPYWPIKLRTSSSHEEGCDRDW
CCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCEECCCCCEEEECCCCCCCCCCCCH
SVATKEFIGENGKVTALKACRVEWKDGKMQEVPGSEFILPADLVLLAMGFTNPVGSMLDA
HHHHHHHHCCCCCEEEEEEEEEECCCCCEECCCCCCEEEHHHHHHHHHCCCCHHHHHHHH
FGVDTDARKNAKAATEGERAYHTNVPKVFAAGDVRRGQSLVVWAIREGRQAARSVDAFLM
HCCCCCCCCCCCCCCCCCCCEECCCCCEEECCCCCCCCEEEEEEEHHHHHHHHHHHHHHC
GHTELPR
CCCCCCC
>Mature Secondary Structure 
GKATGFLEFPRQNEGYEAVVKRVKHYKEFVFALSDSEAKIQGARCMDCGIPFCNNGCPV
CCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCC
NNIIPDFNDLVYRQDWKSAIEVLHQTNNFPEFTGRICPAPCEAACTLGINELPVGIKSIE
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCHHHHCCCHHCCCCHHHHH
HAIIDKAWEEGWVAPQPPKHKTGKTVAVVGSGPAGMAAAQQLARAGHDVTVFEKNDRIGG
HHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCCCC
LLRYGIPDFKMEKTLIDRRIEQMQAEGVTFRAGVMVTDGALPAGIKNYARETISAKDLMD
EEECCCCCHHHHHHHHHHHHHHHHHCCEEEEECEEEECCCCCHHHHHHHHHHCCHHHHHH
QFDAVVLAGGSEVPRDLPVPGRDLDGIHFALEFLIPQNKEVAGDGENEIRAEGKNVIVIG
HHCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHCCCCEEEEEE
GGDTGSDCVGTSNRHGATSVNQFELLPQPPEEEDKPLVWPYWPIKLRTSSSHEEGCDRDW
CCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCEECCCCCEEEECCCCCCCCCCCCH
SVATKEFIGENGKVTALKACRVEWKDGKMQEVPGSEFILPADLVLLAMGFTNPVGSMLDA
HHHHHHHHCCCCCEEEEEEEEEECCCCCEECCCCCCEEEHHHHHHHHHCCCCHHHHHHHH
FGVDTDARKNAKAATEGERAYHTNVPKVFAAGDVRRGQSLVVWAIREGRQAARSVDAFLM
HCCCCCCCCCCCCCCCCCCCEECCCCCEEECCCCCCCCEEEEEEEHHHHHHHHHHHHHHC
GHTELPR
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]