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The map label for this gene is sspA [H]

Identifier: 73542774

GI number: 73542774

Start: 3401892

End: 3402503

Strand: Reverse

Name: sspA [H]

Synonym: Reut_A3090

Alternate gene names: 73542774

Gene position: 3402503-3401892 (Counterclockwise)

Preceding gene: 73542775

Following gene: 73542773

Centisome position: 89.39

GC content: 59.97

Gene sequence:

>612_bases
ATGATGGTGTTGTATTCGGGTACGACTTGCCCGTTTTCCCAACGTTGCCGCCTCGTCCTGTTCGAAAAGGGCATGGACTT
CGAGATCCGCGACGTCGACCTGTTCAACAAGCCCGAAGATATTTCGGTGATGAACCCCTACGGCCAGGTGCCTATCCTGG
TAGAGCGCGACCTCATCCTGTATGAGTCGAACATCATCAACGAGTACATCGACGAGCGCTTCCCGCACCCGCAGCTGATG
CCGGCTGACCCGGTGCAGCGCGCCCGCGCCCGCCTGTTCCTGTTCAACTTTGAAAAGGAACTGTTCACGCACGTGTACGT
GCTCGAGAACGAAAAGGGCAAGGCCGCCGAGAAGAACCACGAGCGCGCCCGCGCCGCGATTCGCGACCGCCTGACGCAGC
TCGCGCCGATCTTCGTCAAGAACAAGTACATGCTTGGGGAAGAGTTCTCGATGCTCGACGTGGCCATCGCCCCGCTGCTG
TGGCGCCTGGACCATTACGGTATCGAACTGTCGAAGAACGCCGCGCCGCTGCTCAAGTACGCCGAGCGCATCTTCAGCCG
TCCGGCCTACATCGAGGCGCTGACGCCGTCGGAAAAGGTTATGCGCCGTTAA

Upstream 100 bases:

>100_bases
TGGATTATATTCAACTTATTTGCTTTTCGATGCCCGGATTTGGCAGAATGTCAGACCTTACCGTCCGACCCAGCATCGGC
TAAGCCCAAGGAATCCAACC

Downstream 100 bases:

>100_bases
GCATGGCGGGCCCGATGCTTGCATCGGGCCAACCACCGGCGCCATGAAGAAGCACCATGCCTGAAACCTCCACCAAGCCC
TATCTGATCCGCGCCATTTA

Product: glutathione S-transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MMVLYSGTTCPFSQRCRLVLFEKGMDFEIRDVDLFNKPEDISVMNPYGQVPILVERDLILYESNIINEYIDERFPHPQLM
PADPVQRARARLFLFNFEKELFTHVYVLENEKGKAAEKNHERARAAIRDRLTQLAPIFVKNKYMLGEEFSMLDVAIAPLL
WRLDHYGIELSKNAAPLLKYAERIFSRPAYIEALTPSEKVMRR

Sequences:

>Translated_203_residues
MMVLYSGTTCPFSQRCRLVLFEKGMDFEIRDVDLFNKPEDISVMNPYGQVPILVERDLILYESNIINEYIDERFPHPQLM
PADPVQRARARLFLFNFEKELFTHVYVLENEKGKAAEKNHERARAAIRDRLTQLAPIFVKNKYMLGEEFSMLDVAIAPLL
WRLDHYGIELSKNAAPLLKYAERIFSRPAYIEALTPSEKVMRR
>Mature_203_residues
MMVLYSGTTCPFSQRCRLVLFEKGMDFEIRDVDLFNKPEDISVMNPYGQVPILVERDLILYESNIINEYIDERFPHPQLM
PADPVQRARARLFLFNFEKELFTHVYVLENEKGKAAEKNHERARAAIRDRLTQLAPIFVKNKYMLGEEFSMLDVAIAPLL
WRLDHYGIELSKNAAPLLKYAERIFSRPAYIEALTPSEKVMRR

Specific function: Forms an equimolar complex with the RNA polymerase holoenzyme (RNAP) but not with the core enzyme [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI4758484, Length=200, Percent_Identity=32.5, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI38016131, Length=184, Percent_Identity=34.2391304347826, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI300360534, Length=194, Percent_Identity=32.4742268041237, Blast_Score=94, Evalue=9e-20,
Organism=Homo sapiens, GI300360569, Length=175, Percent_Identity=34.8571428571429, Blast_Score=93, Evalue=1e-19,
Organism=Homo sapiens, GI300360532, Length=197, Percent_Identity=31.4720812182741, Blast_Score=85, Evalue=5e-17,
Organism=Homo sapiens, GI300360567, Length=180, Percent_Identity=30, Blast_Score=81, Evalue=7e-16,
Organism=Homo sapiens, GI300360571, Length=171, Percent_Identity=30.4093567251462, Blast_Score=79, Evalue=4e-15,
Organism=Escherichia coli, GI1789624, Length=203, Percent_Identity=45.320197044335, Blast_Score=173, Evalue=7e-45,
Organism=Caenorhabditis elegans, GI17551302, Length=198, Percent_Identity=29.7979797979798, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24660979, Length=166, Percent_Identity=31.3253012048193, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI21355779, Length=164, Percent_Identity=30.4878048780488, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: NA

Molecular weight: Translated: 23788; Mature: 23788

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMVLYSGTTCPFSQRCRLVLFEKGMDFEIRDVDLFNKPEDISVMNPYGQVPILVERDLIL
CEEEEECCCCCHHHCCEEEEEECCCCEEEEEEECCCCCCCCEEECCCCCCCEEEECCEEE
YESNIINEYIDERFPHPQLMPADPVQRARARLFLFNFEKELFTHVYVLENEKGKAAEKNH
EHHHHHHHHHHHHCCCCCCCCCCHHHHHHHEEEEEECHHHHHEEEEEEECCCCCCHHHHH
ERARAAIRDRLTQLAPIFVKNKYMLGEEFSMLDVAIAPLLWRLDHYGIELSKNAAPLLKY
HHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHCCEEECCCCHHHHHH
AERIFSRPAYIEALTPSEKVMRR
HHHHHCCCCEEEEECCHHHHHCC
>Mature Secondary Structure
MMVLYSGTTCPFSQRCRLVLFEKGMDFEIRDVDLFNKPEDISVMNPYGQVPILVERDLIL
CEEEEECCCCCHHHCCEEEEEECCCCEEEEEEECCCCCCCCEEECCCCCCCEEEECCEEE
YESNIINEYIDERFPHPQLMPADPVQRARARLFLFNFEKELFTHVYVLENEKGKAAEKNH
EHHHHHHHHHHHHCCCCCCCCCCHHHHHHHEEEEEECHHHHHEEEEEEECCCCCCHHHHH
ERARAAIRDRLTQLAPIFVKNKYMLGEEFSMLDVAIAPLLWRLDHYGIELSKNAAPLLKY
HHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHCCEEECCCCHHHHHH
AERIFSRPAYIEALTPSEKVMRR
HHHHHCCCCEEEEECCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]