Click here to switch to the map view.

The map label for this gene is nac [H]

Identifier: 73542769

GI number: 73542769

Start: 3396862

End: 3397887

Strand: Reverse

Name: nac [H]

Synonym: Reut_A3085

Alternate gene names: 73542769

Gene position: 3397887-3396862 (Counterclockwise)

Preceding gene: 73542773

Following gene: 73542767

Centisome position: 89.26

GC content: 67.64

Gene sequence:

>1026_bases
ATGGGAATACCGGTGGACGTGCGGGGATTGCGCGGCTTTCTCGCCGTGGCGGCGGCGGGATCGATCTCGCGCGCGGCGGA
AACGCTGCATATCGCCCAGCCGGCGCTGAGCTTGCAGATCCGCCAGCTTGAAGACACACTCGGCGTGGCCTTGTTCACGC
GCAGCCACCGGGGCGTGTCGGTCACGGCCGCCGGCCAACGTTTCGAGCACCACGCGCGGGATATCCTGAAGCGGGTCGAC
CTGGCGTGCGAAGACGTCAGGGACCTCGTCACGGACCCGGAAGGCCGCGTCGCCATTGGCCTGCCGCAGTCCATGGCCAA
GATCCTGACCGTGCCGCTGGTGCGGGAGACCATCCGGCGCTGGCCCAAGGTGCGCCTGCAGGTGATCGAGCTGAGCACCG
GTTACATCCCGAACCATTTGCTGACGGGCCATATCGATATCGGCCTGACCTTCCAGGCAAATGCCGACCCCGGCCTCCAG
TTCGATCACCTTGTCGATGAAGACCTTGTGATCGTGGCGGAGCCCGGCCGGTTTGCCGGGGCGAGACGGCGCGACGTCCT
GCCGGCCAACGCGATCCGCCTGCGCGAGCTGAGCGATTACCCCATGATTCTGCCCGCCGGCGAGCATGGCCTGCGCGCGC
TGATCGACAGCCACCTGCGCGCGAACGAGATCGACCTCCACGTACTGGCGGAAGTGAATGCGATTCCCGAACTGATCGCG
CTGGCCGCGGCCGGCGTCGGCTGCACGGTGCTGTCCTACGCTTCGGTCTGCGCGGAGCTGCGCAAGGGTCTGTTGTCGGC
CTCGCGCATTGCGGAGCCCGCCATGTCGCGGCCGGTCTTCCTGTGCCGCTCGGCCACCATGCCACTGTCCGTGGCGGCCT
CGGCGGTGCATGAACTGCTGACGGAAATCGTCAGCGAGCAGATCGGAGACGGCAGCTGGCCGGCGCGCGTCGCTATGGCG
ACAGCACCCGCTCAATCCGCGCGATCTCGCCCTCCATCCAGCGGCGCAGCGACCGATCGGACATGA

Upstream 100 bases:

>100_bases
CTATCCGGGGCGCTGGGCTATATGTCCAAGATATATTTTGGATGCCTGGTATCATGAATCCTTATAGCCAGCGGGCACAC
ACTAGGGTCAGGAGACAGCC

Downstream 100 bases:

>100_bases
CCTGCAACGTGCTGCGACCCAGGATGCGATAGACCGTCTTGCGATCGATCTTCGAGACCTGTGCGGGGTCAAGCCAAACT
TCGTTCCGCACGAGCTGCCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Nitrogen assimilation control protein [H]

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MGIPVDVRGLRGFLAVAAAGSISRAAETLHIAQPALSLQIRQLEDTLGVALFTRSHRGVSVTAAGQRFEHHARDILKRVD
LACEDVRDLVTDPEGRVAIGLPQSMAKILTVPLVRETIRRWPKVRLQVIELSTGYIPNHLLTGHIDIGLTFQANADPGLQ
FDHLVDEDLVIVAEPGRFAGARRRDVLPANAIRLRELSDYPMILPAGEHGLRALIDSHLRANEIDLHVLAEVNAIPELIA
LAAAGVGCTVLSYASVCAELRKGLLSASRIAEPAMSRPVFLCRSATMPLSVAASAVHELLTEIVSEQIGDGSWPARVAMA
TAPAQSARSRPPSSGAATDRT

Sequences:

>Translated_341_residues
MGIPVDVRGLRGFLAVAAAGSISRAAETLHIAQPALSLQIRQLEDTLGVALFTRSHRGVSVTAAGQRFEHHARDILKRVD
LACEDVRDLVTDPEGRVAIGLPQSMAKILTVPLVRETIRRWPKVRLQVIELSTGYIPNHLLTGHIDIGLTFQANADPGLQ
FDHLVDEDLVIVAEPGRFAGARRRDVLPANAIRLRELSDYPMILPAGEHGLRALIDSHLRANEIDLHVLAEVNAIPELIA
LAAAGVGCTVLSYASVCAELRKGLLSASRIAEPAMSRPVFLCRSATMPLSVAASAVHELLTEIVSEQIGDGSWPARVAMA
TAPAQSARSRPPSSGAATDRT
>Mature_340_residues
GIPVDVRGLRGFLAVAAAGSISRAAETLHIAQPALSLQIRQLEDTLGVALFTRSHRGVSVTAAGQRFEHHARDILKRVDL
ACEDVRDLVTDPEGRVAIGLPQSMAKILTVPLVRETIRRWPKVRLQVIELSTGYIPNHLLTGHIDIGLTFQANADPGLQF
DHLVDEDLVIVAEPGRFAGARRRDVLPANAIRLRELSDYPMILPAGEHGLRALIDSHLRANEIDLHVLAEVNAIPELIAL
AAAGVGCTVLSYASVCAELRKGLLSASRIAEPAMSRPVFLCRSATMPLSVAASAVHELLTEIVSEQIGDGSWPARVAMAT
APAQSARSRPPSSGAATDRT

Specific function: Transcriptional activator for the hut, put and ure operons and repressor for the gdh and gltB operons in response to nitrogen limitation. Negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788297, Length=305, Percent_Identity=29.8360655737705, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI157672245, Length=288, Percent_Identity=30.5555555555556, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1787601, Length=208, Percent_Identity=31.25, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI145693105, Length=319, Percent_Identity=25.3918495297806, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI1788887, Length=169, Percent_Identity=36.6863905325444, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1788748, Length=300, Percent_Identity=25, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787879, Length=250, Percent_Identity=32, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI87081904, Length=174, Percent_Identity=32.7586206896552, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87082132, Length=261, Percent_Identity=24.904214559387, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1789204, Length=255, Percent_Identity=25.4901960784314, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787806, Length=249, Percent_Identity=25.7028112449799, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1789440, Length=225, Percent_Identity=26.2222222222222, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1790399, Length=145, Percent_Identity=32.4137931034483, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1786401, Length=163, Percent_Identity=29.4478527607362, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787530, Length=196, Percent_Identity=31.1224489795918, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1787589, Length=253, Percent_Identity=26.8774703557312, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1788706, Length=166, Percent_Identity=29.5180722891566, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1787949, Length=135, Percent_Identity=30.3703703703704, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786713, Length=198, Percent_Identity=30.3030303030303, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI2367136, Length=256, Percent_Identity=26.171875, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1789506, Length=243, Percent_Identity=22.6337448559671, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789173, Length=178, Percent_Identity=28.6516853932584, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787128, Length=161, Percent_Identity=27.3291925465839, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36542; Mature: 36410

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIPVDVRGLRGFLAVAAAGSISRAAETLHIAQPALSLQIRQLEDTLGVALFTRSHRGVS
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHEEEEEECCCCEE
VTAAGQRFEHHARDILKRVDLACEDVRDLVTDPEGRVAIGLPQSMAKILTVPLVRETIRR
EEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHHH
WPKVRLQVIELSTGYIPNHLLTGHIDIGLTFQANADPGLQFDHLVDEDLVIVAEPGRFAG
CCCEEEEEEEEECCCCCCCEEEEEEEEEEEEECCCCCCCCCHHCCCCCEEEEECCCCCCC
ARRRDVLPANAIRLRELSDYPMILPAGEHGLRALIDSHLRANEIDLHVLAEVNAIPELIA
CCCCCCCCCCHHHHEECCCCCEEEECCCHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHH
LAAAGVGCTVLSYASVCAELRKGLLSASRIAEPAMSRPVFLCRSATMPLSVAASAVHELL
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHH
TEIVSEQIGDGSWPARVAMATAPAQSARSRPPSSGAATDRT
HHHHHHHCCCCCCCHHEEEEECCHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
GIPVDVRGLRGFLAVAAAGSISRAAETLHIAQPALSLQIRQLEDTLGVALFTRSHRGVS
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHEEEEEECCCCEE
VTAAGQRFEHHARDILKRVDLACEDVRDLVTDPEGRVAIGLPQSMAKILTVPLVRETIRR
EEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHHH
WPKVRLQVIELSTGYIPNHLLTGHIDIGLTFQANADPGLQFDHLVDEDLVIVAEPGRFAG
CCCEEEEEEEEECCCCCCCEEEEEEEEEEEEECCCCCCCCCHHCCCCCEEEEECCCCCCC
ARRRDVLPANAIRLRELSDYPMILPAGEHGLRALIDSHLRANEIDLHVLAEVNAIPELIA
CCCCCCCCCCHHHHEECCCCCEEEECCCHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHH
LAAAGVGCTVLSYASVCAELRKGLLSASRIAEPAMSRPVFLCRSATMPLSVAASAVHELL
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHH
TEIVSEQIGDGSWPARVAMATAPAQSARSRPPSSGAATDRT
HHHHHHHCCCCCCCHHEEEEECCHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8458853 [H]