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The map label for this gene is clcA [H]

Identifier: 73542739

GI number: 73542739

Start: 3357865

End: 3359385

Strand: Reverse

Name: clcA [H]

Synonym: Reut_A3055

Alternate gene names: 73542739

Gene position: 3359385-3357865 (Counterclockwise)

Preceding gene: 73542740

Following gene: 73542738

Centisome position: 88.25

GC content: 70.09

Gene sequence:

>1521_bases
ATGCCTTGGCCATGCCCCCTCGTCATGACTGACCGCGCCCCCTCCGACCCAGCCGAACCGTCCCCCGGCGCTGACCGCGC
CGACGCTTCCCACGCAGATTCTCCAGACACCGGAACCGATCGCGCGACCGAGCGCGCCGAAGCGCGCCTGTCCGAAGCCA
TCAGCCGCCGCGCGCGCATGGCGGCGTGGCGCAAGTCACGCCAGGCCGGCCGCATCTCTCGCACCACGCTGCGCTATGCC
GTCTTCATGTGCGGCGCCGGCTGCGTGGCGTTGTTCTCGATGGTGTTCGCGTGGATCGCCGAGGTCGCGCTGCGCTGGAA
CCATCACCTGACGCAGAGCACGCCGTGGATCGCATTCGTGATGCTGCCGTTCGGGCTGGCCGCGCTGCGCTGGCTCACCA
TCCGGCTCGCGCCGCAAGCGCGCGGCAGCGGCATACCGCAGGTGATCGCCGCGGTCACGCTGCCGCCGGCAGGACCGGCG
CAGACGGTGCTCGTGTCGTTCCGCCAGTCGATGTGGAAGGTGGTGCTGACCGCTGCGGGCCTCCTCGCCGGGGCATCGGT
CGGACGCGAAGGGCCCTCGGTGCAGGTCGGCGCAGCGGCCATGCTGGCGTGGGGCCGCTGGTGCCACGAAAAGATGCGCT
TTCGCATCGGCTTCCATCCGAACGCTTTGATCGCGGCGGGCGCCGCGGGCGGGCTTGCGGCAGCATTCAACACGCCGCTG
GCCGGCGTGGTGTTCGCGATCGAGGAACTTGGCCGCGGCACGGCCGTGCGCTGGGACCGGCTGGTGCTGTCGGGCGTGCT
GACGGCCGGTTTCCTGTCGCTGGCGCTGCTCGGCAACAACCCGTATTTCGCCGTCAAGGTGCCGATGCTGGTGCTGCGAA
ACGCCTGGGGTCCGGTGCTGCTGTGCGCGGTCGTGAACGGCGTGCTCGGCGGGCTGTTCGCCAAGGGGCTGATCGGCGGC
GTGCCGGGCCTCTTGCCGGTGCGCTGGCGCCACTGGCCGCTCGAGCATCCGGTGCAGGTGGCGTTTGGCTGCGGCCTGGT
CGTGGCGGTACTCGGCTGGGCGACAAGCGGGGCGACATTCGGCACGGGCTACGAACAAGCGTCGGCGCTGATCAACGGCG
AATCGCACGCCACGCTGTGGTTCGGGCTGGCCAAGCTGGTTGCCACCGTGGTGTCGTACTTCGCGGGCATCCCCGGCGGC
ATCTTTACGCCAGCACTGGCCATCGGCGCCGGCATCGGCGAAAACGTCGCGCATTTCATCCCGGGCATGCATGAGCCGCG
CGTGCTCGCGCTCGTGGCCATGGCCGCGTTCCTCGCGGCGGCCACGCAGGCGCCGATCACCGCGAGCGTGATCGTCATGG
AGATGACGCGCACGCAGGACCTGACGCTATTCCTGCTGGCATCGTCGCTGCTGGCATCGTTCATCTCGCGCCAGTTCTCG
CCGCATCCGTTCTACCACCACGTCGGCCATGTGTTCCGGCGCGAGGCGATGGCGCTGGTGCGCAAGGCCCCCGCGAGCTG
A

Upstream 100 bases:

>100_bases
TATGTGGCCGATAACCGGCGCGCGGCCCGCCCCGGGCGGCATGACCGGCGTATAGTGCCGGGACATACCGGCATAGGCGC
CTCCCCCAACACGGCGCAAT

Downstream 100 bases:

>100_bases
CGCTGCGCTATGTGCGCCAAGCCATGGTGGCGCGCGCGGCTTTCCCCTAACGTTGTGGCGCGCCGGTCGCAGGACCGGCT
CGTGTGTAGCGGCGGCACAC

Product: Cl- channel, voltage gated

Products: Cl Ion [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 506; Mature: 505

Protein sequence:

>506_residues
MPWPCPLVMTDRAPSDPAEPSPGADRADASHADSPDTGTDRATERAEARLSEAISRRARMAAWRKSRQAGRISRTTLRYA
VFMCGAGCVALFSMVFAWIAEVALRWNHHLTQSTPWIAFVMLPFGLAALRWLTIRLAPQARGSGIPQVIAAVTLPPAGPA
QTVLVSFRQSMWKVVLTAAGLLAGASVGREGPSVQVGAAAMLAWGRWCHEKMRFRIGFHPNALIAAGAAGGLAAAFNTPL
AGVVFAIEELGRGTAVRWDRLVLSGVLTAGFLSLALLGNNPYFAVKVPMLVLRNAWGPVLLCAVVNGVLGGLFAKGLIGG
VPGLLPVRWRHWPLEHPVQVAFGCGLVVAVLGWATSGATFGTGYEQASALINGESHATLWFGLAKLVATVVSYFAGIPGG
IFTPALAIGAGIGENVAHFIPGMHEPRVLALVAMAAFLAAATQAPITASVIVMEMTRTQDLTLFLLASSLLASFISRQFS
PHPFYHHVGHVFRREAMALVRKAPAS

Sequences:

>Translated_506_residues
MPWPCPLVMTDRAPSDPAEPSPGADRADASHADSPDTGTDRATERAEARLSEAISRRARMAAWRKSRQAGRISRTTLRYA
VFMCGAGCVALFSMVFAWIAEVALRWNHHLTQSTPWIAFVMLPFGLAALRWLTIRLAPQARGSGIPQVIAAVTLPPAGPA
QTVLVSFRQSMWKVVLTAAGLLAGASVGREGPSVQVGAAAMLAWGRWCHEKMRFRIGFHPNALIAAGAAGGLAAAFNTPL
AGVVFAIEELGRGTAVRWDRLVLSGVLTAGFLSLALLGNNPYFAVKVPMLVLRNAWGPVLLCAVVNGVLGGLFAKGLIGG
VPGLLPVRWRHWPLEHPVQVAFGCGLVVAVLGWATSGATFGTGYEQASALINGESHATLWFGLAKLVATVVSYFAGIPGG
IFTPALAIGAGIGENVAHFIPGMHEPRVLALVAMAAFLAAATQAPITASVIVMEMTRTQDLTLFLLASSLLASFISRQFS
PHPFYHHVGHVFRREAMALVRKAPAS
>Mature_505_residues
PWPCPLVMTDRAPSDPAEPSPGADRADASHADSPDTGTDRATERAEARLSEAISRRARMAAWRKSRQAGRISRTTLRYAV
FMCGAGCVALFSMVFAWIAEVALRWNHHLTQSTPWIAFVMLPFGLAALRWLTIRLAPQARGSGIPQVIAAVTLPPAGPAQ
TVLVSFRQSMWKVVLTAAGLLAGASVGREGPSVQVGAAAMLAWGRWCHEKMRFRIGFHPNALIAAGAAGGLAAAFNTPLA
GVVFAIEELGRGTAVRWDRLVLSGVLTAGFLSLALLGNNPYFAVKVPMLVLRNAWGPVLLCAVVNGVLGGLFAKGLIGGV
PGLLPVRWRHWPLEHPVQVAFGCGLVVAVLGWATSGATFGTGYEQASALINGESHATLWFGLAKLVATVVSYFAGIPGGI
FTPALAIGAGIGENVAHFIPGMHEPRVLALVAMAAFLAAATQAPITASVIVMEMTRTQDLTLFLLASSLLASFISRQFSP
HPFYHHVGHVFRREAMALVRKAPAS

Specific function: Proton-coupled chloride transporter. Functions as antiport system and exchanges two chloride ions for 1 proton. Probably acts as an electrical shunt for an outwardly-directed proton pump that is linked to amino acid decarboxylation, as part of the extreme

COG id: COG0038

COG function: function code P; Chloride channel protein EriC

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chloride channel (TC 2.A.49) family. ClcA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786350, Length=390, Percent_Identity=29.7435897435897, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI87081943, Length=257, Percent_Identity=29.1828793774319, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014743
- InterPro:   IPR001807 [H]

Pfam domain/function: PF00654 Voltage_CLC [H]

EC number: NA

Molecular weight: Translated: 53602; Mature: 53471

Theoretical pI: Translated: 10.90; Mature: 10.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPWPCPLVMTDRAPSDPAEPSPGADRADASHADSPDTGTDRATERAEARLSEAISRRARM
CCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AAWRKSRQAGRISRTTLRYAVFMCGAGCVALFSMVFAWIAEVALRWNHHLTQSTPWIAFV
HHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
MLPFGLAALRWLTIRLAPQARGSGIPQVIAAVTLPPAGPAQTVLVSFRQSMWKVVLTAAG
HHHHHHHHHHHHHHEECCCCCCCCCHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHH
LLAGASVGREGPSVQVGAAAMLAWGRWCHEKMRFRIGFHPNALIAAGAAGGLAAAFNTPL
HHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHEEECCCCCCCEECCCCCCHHHHHCCCH
AGVVFAIEELGRGTAVRWDRLVLSGVLTAGFLSLALLGNNPYFAVKVPMLVLRNAWGPVL
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHCCCHHHH
LCAVVNGVLGGLFAKGLIGGVPGLLPVRWRHWPLEHPVQVAFGCGLVVAVLGWATSGATF
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
GTGYEQASALINGESHATLWFGLAKLVATVVSYFAGIPGGIFTPALAIGAGIGENVAHFI
CCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHC
PGMHEPRVLALVAMAAFLAAATQAPITASVIVMEMTRTQDLTLFLLASSLLASFISRQFS
CCCCCCHHHHHHHHHHHHHHHHCCCCCHHEEEEEECCCCCHHHHHHHHHHHHHHHHHCCC
PHPFYHHVGHVFRREAMALVRKAPAS
CCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PWPCPLVMTDRAPSDPAEPSPGADRADASHADSPDTGTDRATERAEARLSEAISRRARM
CCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AAWRKSRQAGRISRTTLRYAVFMCGAGCVALFSMVFAWIAEVALRWNHHLTQSTPWIAFV
HHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
MLPFGLAALRWLTIRLAPQARGSGIPQVIAAVTLPPAGPAQTVLVSFRQSMWKVVLTAAG
HHHHHHHHHHHHHHEECCCCCCCCCHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHH
LLAGASVGREGPSVQVGAAAMLAWGRWCHEKMRFRIGFHPNALIAAGAAGGLAAAFNTPL
HHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHEEECCCCCCCEECCCCCCHHHHHCCCH
AGVVFAIEELGRGTAVRWDRLVLSGVLTAGFLSLALLGNNPYFAVKVPMLVLRNAWGPVL
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHCCCHHHH
LCAVVNGVLGGLFAKGLIGGVPGLLPVRWRHWPLEHPVQVAFGCGLVVAVLGWATSGATF
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
GTGYEQASALINGESHATLWFGLAKLVATVVSYFAGIPGGIFTPALAIGAGIGENVAHFI
CCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHC
PGMHEPRVLALVAMAAFLAAATQAPITASVIVMEMTRTQDLTLFLLASSLLASFISRQFS
CCCCCCHHHHHHHHHHHHHHHHCCCCCHHEEEEEECCCCCHHHHHHHHHHHHHHHHHCCC
PHPFYHHVGHVFRREAMALVRKAPAS
CCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cl Ion [Periplasm] [C]

Specific reaction: Cl Ion [Periplasm] = Cl Ion [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA