BacMap

Definition	Ralstonia eutropha JMP134 chromosome 1, complete sequence.
Accession	NC_007347
Length	3,806,533

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The map label for this gene is fadB1 [H]

Identifier: 73542694

GI number: 73542694

Start: 3313473

End: 3314249

Strand: Reverse

Name: fadB1 [H]

Synonym: Reut_A3010

Alternate gene names: 73542694

Gene position: 3314249-3313473 (Counterclockwise)

Preceding gene: 73542695

Following gene: 73542693

Centisome position: 87.07

GC content: 62.55

Gene sequence:

>777_bases
ATGTCGTACGAAAACATCCTCGTGGAAACGCGCGGCCGCGTCGGGCTGGTCACGCTGCACCGTCCGAAGGCGCTGAATGC
GCTCAATGACGCATTGATGGATGAACTGGGTGCCGCACTGACGGTGTTCGACAAGGACGAGAACATCGGTGCCATCGTTA
TTACCGGCAGCGAAAAGGCGTTCGCGGCCGGCGCCGATATTGGCATGATGGCCAAGTACTCGTTCATGGACGTGTACAAG
GGCGACTACATTACCCGCAACTGGGAAACGATCCGCACCATCCGCAAGCCGGTGATTGCAGCCGTGGCAGGCTACGCGCT
GGGCGGTGGCTCGGAACTCGCGATGATGTGCGACATCATCATTGCCGCTGAAAATGCCCGCTTCGGGCAGCCGGAAGTCA
AGCTCGGCACCATGCCCGGCGCCGGCGGCACCCAGCGCATGCCGCGCGCGGTGTCCAAAGCCAAGGCCATGGACCTGTGC
CTGACCGCGCGCATGATGGATGCCGCCGAAGCGGAGCGGGCCGGCCTTGTCTCGCGCGTGGTGCCGGCGGACAAGCTGCT
GGATGAAGCGCTCGCAGCGGCGGAGACCATCGCCGGCTACTCGCTGCCTGTGGTCATGATGATCAAGGAATCGATCAACG
CGGCCTATGAGACAACGCTCGCTGAGGGCGTGCGCTTCGAGCGCCGGCTCTTCCATGCGACGTTTGCCAGCGAAGACCAG
AAGGAAGGCATGGCTGCTTTTGTCGAGAAACGCAGCCCTGACTTCAAGCATCGCTGA

Upstream 100 bases:

>100_bases
AATGCCCGCGGCGTCGCACCTGCCACTCCGCGCGGATAGTCCCAATTGCCGCGTCAACGTGGCAAACTCTCCGGCTGCAT
TTGCCTACAAAGGAGAAGAC

Downstream 100 bases:

>100_bases
TAACTTGATAGGGGAATACCCGAGGTTTTCTCAGGGGTATTCAGTTTCCCGGTGCTAAAAAGAACGCCGTATTCCCTTAT
CGATCAAAGAGTTGGGTGAA

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MSYENILVETRGRVGLVTLHRPKALNALNDALMDELGAALTVFDKDENIGAIVITGSEKAFAAGADIGMMAKYSFMDVYK
GDYITRNWETIRTIRKPVIAAVAGYALGGGSELAMMCDIIIAAENARFGQPEVKLGTMPGAGGTQRMPRAVSKAKAMDLC
LTARMMDAAEAERAGLVSRVVPADKLLDEALAAAETIAGYSLPVVMMIKESINAAYETTLAEGVRFERRLFHATFASEDQ
KEGMAAFVEKRSPDFKHR

Sequences:

>Translated_258_residues
MSYENILVETRGRVGLVTLHRPKALNALNDALMDELGAALTVFDKDENIGAIVITGSEKAFAAGADIGMMAKYSFMDVYK
GDYITRNWETIRTIRKPVIAAVAGYALGGGSELAMMCDIIIAAENARFGQPEVKLGTMPGAGGTQRMPRAVSKAKAMDLC
LTARMMDAAEAERAGLVSRVVPADKLLDEALAAAETIAGYSLPVVMMIKESINAAYETTLAEGVRFERRLFHATFASEDQ
KEGMAAFVEKRSPDFKHR
>Mature_257_residues
SYENILVETRGRVGLVTLHRPKALNALNDALMDELGAALTVFDKDENIGAIVITGSEKAFAAGADIGMMAKYSFMDVYKG
DYITRNWETIRTIRKPVIAAVAGYALGGGSELAMMCDIIIAAENARFGQPEVKLGTMPGAGGTQRMPRAVSKAKAMDLCL
TARMMDAAEAERAGLVSRVVPADKLLDEALAAAETIAGYSLPVVMMIKESINAAYETTLAEGVRFERRLFHATFASEDQK
EGMAAFVEKRSPDFKHR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=260, Percent_Identity=55, Blast_Score=294, Evalue=4e-80,
Organism=Homo sapiens, GI4502327, Length=256, Percent_Identity=37.109375, Blast_Score=118, Evalue=5e-27,
Organism=Homo sapiens, GI70995211, Length=266, Percent_Identity=33.8345864661654, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI68989263, Length=228, Percent_Identity=34.2105263157895, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI20127408, Length=177, Percent_Identity=35.5932203389831, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI31542718, Length=245, Percent_Identity=29.3877551020408, Blast_Score=101, Evalue=8e-22,
Organism=Homo sapiens, GI37594469, Length=212, Percent_Identity=30.6603773584906, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI37594471, Length=212, Percent_Identity=30.6603773584906, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI150378541, Length=244, Percent_Identity=30.7377049180328, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI4757968, Length=266, Percent_Identity=24.4360902255639, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI49355787, Length=266, Percent_Identity=24.4360902255639, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI25453481, Length=265, Percent_Identity=24.5283018867925, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI51538573, Length=265, Percent_Identity=24.5283018867925, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI4757966, Length=216, Percent_Identity=26.3888888888889, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI51538581, Length=216, Percent_Identity=26.3888888888889, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI260274832, Length=260, Percent_Identity=24.2307692307692, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI45643119, Length=260, Percent_Identity=24.2307692307692, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI260275230, Length=260, Percent_Identity=24.2307692307692, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI157694516, Length=247, Percent_Identity=24.2914979757085, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI213417737, Length=247, Percent_Identity=24.2914979757085, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI221136756, Length=259, Percent_Identity=23.5521235521236, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI221136753, Length=259, Percent_Identity=23.5521235521236, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI261878539, Length=135, Percent_Identity=34.0740740740741, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI221307494, Length=259, Percent_Identity=23.5521235521236, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1787659, Length=253, Percent_Identity=44.6640316205534, Blast_Score=214, Evalue=5e-57,
Organism=Escherichia coli, GI221142681, Length=260, Percent_Identity=36.5384615384615, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1788597, Length=264, Percent_Identity=36.3636363636364, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1787660, Length=266, Percent_Identity=32.7067669172932, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI87082183, Length=260, Percent_Identity=31.1538461538462, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI1788682, Length=201, Percent_Identity=31.3432835820896, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1790281, Length=175, Percent_Identity=33.1428571428571, Blast_Score=95, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17554946, Length=251, Percent_Identity=56.1752988047809, Blast_Score=291, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI25145438, Length=243, Percent_Identity=55.1440329218107, Blast_Score=275, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI17540306, Length=240, Percent_Identity=34.1666666666667, Blast_Score=127, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17560910, Length=252, Percent_Identity=30.952380952381, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17540714, Length=220, Percent_Identity=30, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17558304, Length=201, Percent_Identity=35.3233830845771, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17534483, Length=256, Percent_Identity=28.90625, Blast_Score=104, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI25144276, Length=182, Percent_Identity=37.3626373626374, Blast_Score=103, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17508951, Length=182, Percent_Identity=37.3626373626374, Blast_Score=103, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17508953, Length=182, Percent_Identity=37.3626373626374, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI25144160, Length=192, Percent_Identity=31.7708333333333, Blast_Score=88, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI25144157, Length=192, Percent_Identity=31.7708333333333, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17536985, Length=265, Percent_Identity=27.5471698113208, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17549921, Length=179, Percent_Identity=31.2849162011173, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI25141363, Length=200, Percent_Identity=29.5, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17535521, Length=259, Percent_Identity=25.4826254826255, Blast_Score=74, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6320241, Length=194, Percent_Identity=29.3814432989691, Blast_Score=94, Evalue=2e-20,
Organism=Drosophila melanogaster, GI20129971, Length=259, Percent_Identity=56.3706563706564, Blast_Score=301, Evalue=4e-82,
Organism=Drosophila melanogaster, GI24653477, Length=259, Percent_Identity=56.3706563706564, Blast_Score=301, Evalue=4e-82,
Organism=Drosophila melanogaster, GI24653139, Length=266, Percent_Identity=36.4661654135338, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI19920382, Length=249, Percent_Identity=34.9397590361446, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI21357171, Length=260, Percent_Identity=29.6153846153846, Blast_Score=111, Evalue=6e-25,
Organism=Drosophila melanogaster, GI19922422, Length=254, Percent_Identity=28.3464566929134, Blast_Score=104, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24650670, Length=213, Percent_Identity=28.6384976525822, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24654903, Length=187, Percent_Identity=28.8770053475936, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI19921000, Length=174, Percent_Identity=32.7586206896552, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24583077, Length=174, Percent_Identity=32.7586206896552, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24583079, Length=174, Percent_Identity=32.7586206896552, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI28571730, Length=245, Percent_Identity=25.3061224489796, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI28571729, Length=245, Percent_Identity=25.3061224489796, Blast_Score=74, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27905; Mature: 27773

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYENILVETRGRVGLVTLHRPKALNALNDALMDELGAALTVFDKDENIGAIVITGSEKA
CCCCCEEEECCCCEEEEEEECCHHHHHHHHHHHHHHCCEEEEEECCCCCEEEEEECCCCC
FAAGADIGMMAKYSFMDVYKGDYITRNWETIRTIRKPVIAAVAGYALGGGSELAMMCDII
EECCCCCCHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHEEE
IAAENARFGQPEVKLGTMPGAGGTQRMPRAVSKAKAMDLCLTARMMDAAEAERAGLVSRV
EEECCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPADKLLDEALAAAETIAGYSLPVVMMIKESINAAYETTLAEGVRFERRLFHATFASEDQ
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
KEGMAAFVEKRSPDFKHR
HHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SYENILVETRGRVGLVTLHRPKALNALNDALMDELGAALTVFDKDENIGAIVITGSEKA
CCCCEEEECCCCEEEEEEECCHHHHHHHHHHHHHHCCEEEEEECCCCCEEEEEECCCCC
FAAGADIGMMAKYSFMDVYKGDYITRNWETIRTIRKPVIAAVAGYALGGGSELAMMCDII
EECCCCCCHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHEEE
IAAENARFGQPEVKLGTMPGAGGTQRMPRAVSKAKAMDLCLTARMMDAAEAERAGLVSRV
EEECCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPADKLLDEALAAAETIAGYSLPVVMMIKESINAAYETTLAEGVRFERRLFHATFASEDQ
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
KEGMAAFVEKRSPDFKHR
HHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7751302; 11481430 [H]