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The map label for this gene is mraW [H]

Identifier: 73542671

GI number: 73542671

Start: 3277877

End: 3278875

Strand: Reverse

Name: mraW [H]

Synonym: Reut_A2987

Alternate gene names: 73542671

Gene position: 3278875-3277877 (Counterclockwise)

Preceding gene: 73542672

Following gene: 73542670

Centisome position: 86.14

GC content: 67.37

Gene sequence:

>999_bases
ATGAGTCCTACCGGATCGCCGGCAACCCCCGCCCTGCGCCATCGCACCGTGCTGCTGGACGAGGCCGTCGAGGCATTGGT
CTGGCGGCCCGACGGTGTTTACGTGGACGGCACCTTCGGCAGGGGAGGGCACAGCCGGGCAGTTCTGGCCCGGCTGGGGC
CAGACGGGGCCCTCGTCGCGTTCGACAAGGACCCAGCAGCAATCGCAGAAGCGGGCACCATCCAGGATGCCCGCTTCTCC
ATTGAGCACGAGAGCTTTGCGGCCATGGGCGAGCGCCTGGCCGGGCGCGGCCATGTGGCCGGCGTGCTGCTCGACCTGGG
GATCAGCTCGCCCCAGATCGATGAAGCGGCGAGGGGGTTTTCCTTTCGTTTCGAGGGCCCGCTGGACATGCGCATGGACA
CCACGCGCGGCATTACCGCCGCCGAGTGGCTGGCGCAGGCGGAAGAGCAGGACATTGCCAGGGTGATACGAGACTATGGG
GAAGAACGGTTTGCTGTACAGATTGCAAAGGCGATTGTTGCTCGCCGGCGCGAACCCGGCGATGGCGGACCTCTCGCCAC
TACTGCCGACCTTGCCGCGCTCGTGGCGAAAGCCGTCAAAACACGCGAGAAGGGTCAGGACCCTGCGACCCGCACCTTTC
AGGCTCTACGGATTCACATCAATCAAGAGCTTGAGGACCTCGAAAGAGGGCTGAAGGCTGCCTATGACCTGCTGCAGGTA
GGGGGCCGCCTCGTGGTGATCAGCTTCCATTCGCTGGAGGACCGCATCGTCAAGCGGTTCATGGCGGCGCACGCCCGTCC
CCAGCAGGATGCCGATCCCGCGCTGCGCCGCGCGCCACTGCGCGCGGCCGATTTGCCACAGCCGACGCTGCGCCTGCTGG
GCCGCGTCAAGCCCGGCGCGCAGGAAGTTGCCGACAACCCGCGCGCGCGATCGGCCGTGATGCGCGTCGCCGAAAAACTG
GCACCGGCCGCATCGGGCCGAGGGGCGGCGCCAGCATGA

Upstream 100 bases:

>100_bases
TTCGAAGTCTGGGATGCCGCGACATACGCCGCCAAGGAACAGCAGGCGATGGCGCAAGGCATGCCGGAAGCATTGAAGAA
TTTCTCTTTCTGACGAGGTC

Downstream 100 bases:

>100_bases
ACCGACTAACATTTTTCCTCCTTGCGGCACTGATCTTCTGTGCGCTCTCGCTCGTAAGCGCGCAGCACCAGGCGCGCACG
CTGTTCGTCGCGCTCGAGCG

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH [H]

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MSPTGSPATPALRHRTVLLDEAVEALVWRPDGVYVDGTFGRGGHSRAVLARLGPDGALVAFDKDPAAIAEAGTIQDARFS
IEHESFAAMGERLAGRGHVAGVLLDLGISSPQIDEAARGFSFRFEGPLDMRMDTTRGITAAEWLAQAEEQDIARVIRDYG
EERFAVQIAKAIVARRREPGDGGPLATTADLAALVAKAVKTREKGQDPATRTFQALRIHINQELEDLERGLKAAYDLLQV
GGRLVVISFHSLEDRIVKRFMAAHARPQQDADPALRRAPLRAADLPQPTLRLLGRVKPGAQEVADNPRARSAVMRVAEKL
APAASGRGAAPA

Sequences:

>Translated_332_residues
MSPTGSPATPALRHRTVLLDEAVEALVWRPDGVYVDGTFGRGGHSRAVLARLGPDGALVAFDKDPAAIAEAGTIQDARFS
IEHESFAAMGERLAGRGHVAGVLLDLGISSPQIDEAARGFSFRFEGPLDMRMDTTRGITAAEWLAQAEEQDIARVIRDYG
EERFAVQIAKAIVARRREPGDGGPLATTADLAALVAKAVKTREKGQDPATRTFQALRIHINQELEDLERGLKAAYDLLQV
GGRLVVISFHSLEDRIVKRFMAAHARPQQDADPALRRAPLRAADLPQPTLRLLGRVKPGAQEVADNPRARSAVMRVAEKL
APAASGRGAAPA
>Mature_331_residues
SPTGSPATPALRHRTVLLDEAVEALVWRPDGVYVDGTFGRGGHSRAVLARLGPDGALVAFDKDPAAIAEAGTIQDARFSI
EHESFAAMGERLAGRGHVAGVLLDLGISSPQIDEAARGFSFRFEGPLDMRMDTTRGITAAEWLAQAEEQDIARVIRDYGE
ERFAVQIAKAIVARRREPGDGGPLATTADLAALVAKAVKTREKGQDPATRTFQALRIHINQELEDLERGLKAAYDLLQVG
GRLVVISFHSLEDRIVKRFMAAHARPQQDADPALRRAPLRAADLPQPTLRLLGRVKPGAQEVADNPRARSAVMRVAEKLA
PAASGRGAAPA

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA [H]

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family [H]

Homologues:

Organism=Homo sapiens, GI165377209, Length=355, Percent_Identity=38.3098591549296, Blast_Score=188, Evalue=7e-48,
Organism=Homo sapiens, GI165377202, Length=206, Percent_Identity=37.8640776699029, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI1786270, Length=311, Percent_Identity=57.556270096463, Blast_Score=337, Evalue=6e-94,
Organism=Drosophila melanogaster, GI62472493, Length=332, Percent_Identity=38.2530120481928, Blast_Score=184, Evalue=9e-47,
Organism=Drosophila melanogaster, GI28571637, Length=332, Percent_Identity=38.2530120481928, Blast_Score=184, Evalue=9e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002903 [H]

Pfam domain/function: PF01795 Methyltransf_5 [H]

EC number: NA

Molecular weight: Translated: 35657; Mature: 35526

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPTGSPATPALRHRTVLLDEAVEALVWRPDGVYVDGTFGRGGHSRAVLARLGPDGALVA
CCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCEEEECCCCCCCCCCEEEEEECCCCEEEE
FDKDPAAIAEAGTIQDARFSIEHESFAAMGERLAGRGHVAGVLLDLGISSPQIDEAARGF
ECCCCHHHHHCCCCCHHHEECCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHCCC
SFRFEGPLDMRMDTTRGITAAEWLAQAEEQDIARVIRDYGEERFAVQIAKAIVARRREPG
CEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCC
DGGPLATTADLAALVAKAVKTREKGQDPATRTFQALRIHINQELEDLERGLKAAYDLLQV
CCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GGRLVVISFHSLEDRIVKRFMAAHARPQQDADPALRRAPLRAADLPQPTLRLLGRVKPGA
CCEEEEEEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHCCCCCH
QEVADNPRARSAVMRVAEKLAPAASGRGAAPA
HHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SPTGSPATPALRHRTVLLDEAVEALVWRPDGVYVDGTFGRGGHSRAVLARLGPDGALVA
CCCCCCCCHHHHHHHHHHHHHHHHHEECCCCEEEECCCCCCCCCCEEEEEECCCCEEEE
FDKDPAAIAEAGTIQDARFSIEHESFAAMGERLAGRGHVAGVLLDLGISSPQIDEAARGF
ECCCCHHHHHCCCCCHHHEECCHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHCCC
SFRFEGPLDMRMDTTRGITAAEWLAQAEEQDIARVIRDYGEERFAVQIAKAIVARRREPG
CEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCC
DGGPLATTADLAALVAKAVKTREKGQDPATRTFQALRIHINQELEDLERGLKAAYDLLQV
CCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GGRLVVISFHSLEDRIVKRFMAAHARPQQDADPALRRAPLRAADLPQPTLRLLGRVKPGA
CCEEEEEEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHCCCCCH
QEVADNPRARSAVMRVAEKLAPAASGRGAAPA
HHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA