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The map label for this gene is murD

Identifier: 73542665

GI number: 73542665

Start: 3270042

End: 3271553

Strand: Reverse

Name: murD

Synonym: Reut_A2981

Alternate gene names: 73542665

Gene position: 3271553-3270042 (Counterclockwise)

Preceding gene: 73542666

Following gene: 73542664

Centisome position: 85.95

GC content: 69.38

Gene sequence:

>1512_bases
GTGTTTGGCGAGCTGCAAAAACCTCATGTGCTGGTACTGGGCCTCGGCGAATCGGGGCTGGCCATGGCGCGCTGGTGTGG
CCTGAACGGCTGCGCCGTGCGCGTGGCCGACACGCGCGAGGCACCCGCCAACCTTGTCTTCCTGCAGGCCGAACTGATGT
CGGCCGAGTTCGTGGGCGGCCCGTTCGCCGAAAGCCTGCTCGACGGCATCGGCCTGGTGGCGATCAGCCCGGGCTTGTCG
CCGCTGGAGGCGAATACCGGCGAGCTGCTGGCCGCGGCACGCCAGCGCGGTATCCCCGTGTGGGGCGAGATCGAACTGTT
CGCGCGCGCGCTCGCGCACCTGCAGGCCGAATCCGGCTATGCGCCGAAGGTGCTCGCGATCACCGGCACCAACGGCAAGA
CGACTACGACCGCGCTGACCGGCCGGCTGGTCGAGCGCGCCGGCAAGAGCGTGGCGGTGGCCGGCAATATCAGCCCGTCG
GCGCTCGACAAGCTGTCGGCGTGCATTGCTTCGGCCACGCTGCCGGACGTCTGGGTGCTCGAGTTGTCGAGCTTCCAGCT
CGAAACGACGCATACGCTGGCGCCGCACGCGGCGACCGTGCTCAACGTCACGCAGGATCACCTCGACTGGCATGGCTCGA
TGGAAGCCTATGCCGCGTCCAAGGCGCGCATCTTCGGACCGGCCGGCACGGCTTGCGTGCAGGTGCTGAACCGCAATGAC
CGCCTGACCATGGACATGGCCCGCCCGGGCACGGCACCGGTGACCTTCGGCACCGACCTGCCCGAGACGCCGGGCAGCTT
CGGCGTGCTGCGCGAAGGCGGCATGCCGTGGCTGGTGCTCGCCGAACCCGACTCGGAAGCGGAAGGGGAGGGCAAGCCGC
GCCGCCGCAAGGCCGATGCCACCGCGCAGGAGGCCGTGCCGGTGCGCCACAAGCGCCTGATGCCGGCCGACGCGCTGCAT
ATCCGCGGCATGCACAACGCTACCAACGCCATGGCGGCGCTGGCGCTGTGCCGCGCGATCGACCTGCCGCTCAACGCGCT
GCTGCATGGCCTGCGCGAATATCGTGGCGAGCCGCATCGCGTGGAGTGGGTCGCCACTATCGATGAAGTCGAGTATTTCG
ACGACAGCAAGGGCACCAACGTGGGCGCCACGGTGGCCGCGCTGTCGGGTCTGGACAAGCGCGTCGTGCTGATTGCCGGC
GGCGAAGGCAAGGGTCAGGATTTCTCGCCGCTGGCCGCGCCGGTCGCGCAGTACGCGCGCGCCGTGGTGCTGATCGGCCG
TGCAGCGGGCGAGTTGCGCGATGCGCTGCAAGGCAGCGGCGCGTCGCTGGTCGACGCGGCCACGCTTGAAGAAGCCGTCA
ACAAGGCGGCGGAGCTGGCTGAAGGCGGCGACGTTGTGCTCCTGTCGCCGGCCTGCGCCAGCCTCGACATGTTCCGTAAC
TACGTGCACCGCGCCGAAGTGTTCCGCTCGGCGGTGGAAGAACTGGCCCTGTCGCGGGGGATCATGCCATGA

Upstream 100 bases:

>100_bases
CTGCTGGTGCTGATCGGGCTGTCCACGCTGAAGCTGCGCTGATTCGGGCGCTTTCTATATAGACAACAGGATTACTGAAC
TCAAAAGGCAGGAATACGAA

Downstream 100 bases:

>100_bases
GTGAATCGCAGGTCAAGCGCAGCGGTTTCATCGGCGCCACCATCGGCAATGCCTGGGGTGGCCTGCGCGATGCGGTGTCC
GGCGTCAAGCCGACCCGTTC

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MFGELQKPHVLVLGLGESGLAMARWCGLNGCAVRVADTREAPANLVFLQAELMSAEFVGGPFAESLLDGIGLVAISPGLS
PLEANTGELLAAARQRGIPVWGEIELFARALAHLQAESGYAPKVLAITGTNGKTTTTALTGRLVERAGKSVAVAGNISPS
ALDKLSACIASATLPDVWVLELSSFQLETTHTLAPHAATVLNVTQDHLDWHGSMEAYAASKARIFGPAGTACVQVLNRND
RLTMDMARPGTAPVTFGTDLPETPGSFGVLREGGMPWLVLAEPDSEAEGEGKPRRRKADATAQEAVPVRHKRLMPADALH
IRGMHNATNAMAALALCRAIDLPLNALLHGLREYRGEPHRVEWVATIDEVEYFDDSKGTNVGATVAALSGLDKRVVLIAG
GEGKGQDFSPLAAPVAQYARAVVLIGRAAGELRDALQGSGASLVDAATLEEAVNKAAELAEGGDVVLLSPACASLDMFRN
YVHRAEVFRSAVEELALSRGIMP

Sequences:

>Translated_503_residues
MFGELQKPHVLVLGLGESGLAMARWCGLNGCAVRVADTREAPANLVFLQAELMSAEFVGGPFAESLLDGIGLVAISPGLS
PLEANTGELLAAARQRGIPVWGEIELFARALAHLQAESGYAPKVLAITGTNGKTTTTALTGRLVERAGKSVAVAGNISPS
ALDKLSACIASATLPDVWVLELSSFQLETTHTLAPHAATVLNVTQDHLDWHGSMEAYAASKARIFGPAGTACVQVLNRND
RLTMDMARPGTAPVTFGTDLPETPGSFGVLREGGMPWLVLAEPDSEAEGEGKPRRRKADATAQEAVPVRHKRLMPADALH
IRGMHNATNAMAALALCRAIDLPLNALLHGLREYRGEPHRVEWVATIDEVEYFDDSKGTNVGATVAALSGLDKRVVLIAG
GEGKGQDFSPLAAPVAQYARAVVLIGRAAGELRDALQGSGASLVDAATLEEAVNKAAELAEGGDVVLLSPACASLDMFRN
YVHRAEVFRSAVEELALSRGIMP
>Mature_503_residues
MFGELQKPHVLVLGLGESGLAMARWCGLNGCAVRVADTREAPANLVFLQAELMSAEFVGGPFAESLLDGIGLVAISPGLS
PLEANTGELLAAARQRGIPVWGEIELFARALAHLQAESGYAPKVLAITGTNGKTTTTALTGRLVERAGKSVAVAGNISPS
ALDKLSACIASATLPDVWVLELSSFQLETTHTLAPHAATVLNVTQDHLDWHGSMEAYAASKARIFGPAGTACVQVLNRND
RLTMDMARPGTAPVTFGTDLPETPGSFGVLREGGMPWLVLAEPDSEAEGEGKPRRRKADATAQEAVPVRHKRLMPADALH
IRGMHNATNAMAALALCRAIDLPLNALLHGLREYRGEPHRVEWVATIDEVEYFDDSKGTNVGATVAALSGLDKRVVLIAG
GEGKGQDFSPLAAPVAQYARAVVLIGRAAGELRDALQGSGASLVDAATLEEAVNKAAELAEGGDVVLLSPACASLDMFRN
YVHRAEVFRSAVEELALSRGIMP

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=496, Percent_Identity=36.0887096774194, Blast_Score=216, Evalue=4e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_CUPPJ (Q46WZ2)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_297185.1
- ProteinModelPortal:   Q46WZ2
- SMR:   Q46WZ2
- GeneID:   3612378
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A2981
- NMPDR:   fig|264198.3.peg.3102
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- ProtClustDB:   PRK02006
- BioCyc:   REUT264198:REUT_A2981-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 52887; Mature: 52887

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGELQKPHVLVLGLGESGLAMARWCGLNGCAVRVADTREAPANLVFLQAELMSAEFVGG
CCCCCCCCEEEEEECCCCCCHHHHHCCCCCCEEEEECCCCCCCCEEEEEHHHHHHHHCCC
PFAESLLDGIGLVAISPGLSPLEANTGELLAAARQRGIPVWGEIELFARALAHLQAESGY
HHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCC
APKVLAITGTNGKTTTTALTGRLVERAGKSVAVAGNISPSALDKLSACIASATLPDVWVL
CCEEEEEECCCCCEEHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEE
ELSSFQLETTHTLAPHAATVLNVTQDHLDWHGSMEAYAASKARIFGPAGTACVQVLNRND
EECCEEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHCCCCEEECCCHHHHHHHHCCCC
RLTMDMARPGTAPVTFGTDLPETPGSFGVLREGGMPWLVLAEPDSEAEGEGKPRRRKADA
CEEEEECCCCCCCEEECCCCCCCCCCCCEEECCCCCEEEEECCCCCCCCCCCCCHHCCCC
TAQEAVPVRHKRLMPADALHIRGMHNATNAMAALALCRAIDLPLNALLHGLREYRGEPHR
CHHHCCCHHHHHCCCCCHHEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCE
VEWVATIDEVEYFDDSKGTNVGATVAALSGLDKRVVLIAGGEGKGQDFSPLAAPVAQYAR
EEEEEEEHHHHHCCCCCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHH
AVVLIGRAAGELRDALQGSGASLVDAATLEEAVNKAAELAEGGDVVLLSPACASLDMFRN
HEEEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHH
YVHRAEVFRSAVEELALSRGIMP
HHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFGELQKPHVLVLGLGESGLAMARWCGLNGCAVRVADTREAPANLVFLQAELMSAEFVGG
CCCCCCCCEEEEEECCCCCCHHHHHCCCCCCEEEEECCCCCCCCEEEEEHHHHHHHHCCC
PFAESLLDGIGLVAISPGLSPLEANTGELLAAARQRGIPVWGEIELFARALAHLQAESGY
HHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCC
APKVLAITGTNGKTTTTALTGRLVERAGKSVAVAGNISPSALDKLSACIASATLPDVWVL
CCEEEEEECCCCCEEHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEE
ELSSFQLETTHTLAPHAATVLNVTQDHLDWHGSMEAYAASKARIFGPAGTACVQVLNRND
EECCEEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHCCCCEEECCCHHHHHHHHCCCC
RLTMDMARPGTAPVTFGTDLPETPGSFGVLREGGMPWLVLAEPDSEAEGEGKPRRRKADA
CEEEEECCCCCCCEEECCCCCCCCCCCCEEECCCCCEEEEECCCCCCCCCCCCCHHCCCC
TAQEAVPVRHKRLMPADALHIRGMHNATNAMAALALCRAIDLPLNALLHGLREYRGEPHR
CHHHCCCHHHHHCCCCCHHEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCE
VEWVATIDEVEYFDDSKGTNVGATVAALSGLDKRVVLIAGGEGKGQDFSPLAAPVAQYAR
EEEEEEEHHHHHCCCCCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHH
AVVLIGRAAGELRDALQGSGASLVDAATLEEAVNKAAELAEGGDVVLLSPACASLDMFRN
HEEEEEHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHH
YVHRAEVFRSAVEELALSRGIMP
HHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA