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The map label for this gene is nrdB [H]

Identifier: 73542624

GI number: 73542624

Start: 3222450

End: 3223634

Strand: Reverse

Name: nrdB [H]

Synonym: Reut_A2940

Alternate gene names: 73542624

Gene position: 3223634-3222450 (Counterclockwise)

Preceding gene: 73542625

Following gene: 73542623

Centisome position: 84.69

GC content: 59.58

Gene sequence:

>1185_bases
ATGCTGAGCTGGGACGACGACGTTCAAGCCACCCCGCAGGCCGCACCGCAGCCTGCCCTGCAACCCGCTGCCGCCGTCAC
GGCTGACCAGCAAGGCGTGCTGCCGTCGAGCGCCACGCAAGCCGGCATCCTGGGCAACAACCCCAACGCCGCCGCCGCGC
AAAGCAACCGCCGCGTCAACGCCGCCGACAAGCGCGTCATCAACGGCTCGACCGACGTCAATCAGCTCGTCCCGTTCAAG
TACAAGTGGGCGTGGGAAAAGTACCTGGCAGGCTGCGCCAACCACTGGATGCCGCAGGAAATCAACATGTCGCGCGACAT
CGCGACGTGGAAAGACCCCAACGGCCTGACCGAAGACGAGCGCCGCATCATCAAGCGCAACCTCGGCTTCTTCGTCACCG
CCGACTCGCTGGCCGCCAACAACATCGTGCTGGGCACCTACCGCCAGATCACCGCGCCGGAATGCCGCCAGTACCTGCTG
CGCCAGGCCTTTGAAGAGGCCATCCACACGCACGCCTACCAGTACATCGTTGAATCGCTGGGCCTGAACGAAGCCGAGAT
CTTCAACGCGTACCACGAAGTGCAGTCGATCCGCGACAAGGACGAGTTCCTGATCCCGTTCATCGACACGCTGACCGACC
CGTCGTTCAAGACCGGCACGCCGGAAAACGACCAGAAGCTGCTGAAGTCGCTGATCGTGTTCGCCTGCATCATGGAAGGG
CTGTTCTTCTATGTGGGCTTCACGCAGATTTTGGCGATGGGGCGGCAGAACAAGATGACTGGGGCTGCGGAGCAGTATCA
GTACATCCTGCGGGATGAGTCGCTGCACTGCAATTTTGGTATCGACCTGATTAATCAGATCAAGCTGGAGAATCCGCATC
TTTGGACGGCGGAGTTCAAGGCTGAAATCACCGAGCTGTTCAAGAAGGCTGTTGATCTGGAGTATCGGTACGCTGAGGAC
ACCATGCCGCGCGGCGTGCTGGGGCTGAATGCGCCGATGTTCAAGTCGTATCTGCGGTTTATTTGCAATCGTCGGTGCCA
GCAGATTGGGTTGGATCAGCTGTTCCCGAATGAGGAGAATCCGTTCCCGTGGATGAGCGAGATGATCGATCTGAAGAAGG
AGCGGAACTTCTTTGAGACTCGGGTTATTGAGTATCAGACGGGTGGGGCGTTGAGCTGGGATTGA

Upstream 100 bases:

>100_bases
GCATGCGGGAGAGGGGCCGGGGGAGAGGGCCGGCGCCACCACCACGTGAGCCGTCAACATTGACCACCGCGCCCCCCAAT
CGATCACGGAGAACCACATC

Downstream 100 bases:

>100_bases
GGTTGTTGTTGGTGGACGTAGAAATGGGAGCATGGTGCTCCCATTTTTGTTTGTGGCCAGACATGCTAGTCCTTGCTTTG
TAAGAATCTGAAGGCTGGGT

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Ribonucleotide reductase small subunit [H]

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MLSWDDDVQATPQAAPQPALQPAAAVTADQQGVLPSSATQAGILGNNPNAAAAQSNRRVNAADKRVINGSTDVNQLVPFK
YKWAWEKYLAGCANHWMPQEINMSRDIATWKDPNGLTEDERRIIKRNLGFFVTADSLAANNIVLGTYRQITAPECRQYLL
RQAFEEAIHTHAYQYIVESLGLNEAEIFNAYHEVQSIRDKDEFLIPFIDTLTDPSFKTGTPENDQKLLKSLIVFACIMEG
LFFYVGFTQILAMGRQNKMTGAAEQYQYILRDESLHCNFGIDLINQIKLENPHLWTAEFKAEITELFKKAVDLEYRYAED
TMPRGVLGLNAPMFKSYLRFICNRRCQQIGLDQLFPNEENPFPWMSEMIDLKKERNFFETRVIEYQTGGALSWD

Sequences:

>Translated_394_residues
MLSWDDDVQATPQAAPQPALQPAAAVTADQQGVLPSSATQAGILGNNPNAAAAQSNRRVNAADKRVINGSTDVNQLVPFK
YKWAWEKYLAGCANHWMPQEINMSRDIATWKDPNGLTEDERRIIKRNLGFFVTADSLAANNIVLGTYRQITAPECRQYLL
RQAFEEAIHTHAYQYIVESLGLNEAEIFNAYHEVQSIRDKDEFLIPFIDTLTDPSFKTGTPENDQKLLKSLIVFACIMEG
LFFYVGFTQILAMGRQNKMTGAAEQYQYILRDESLHCNFGIDLINQIKLENPHLWTAEFKAEITELFKKAVDLEYRYAED
TMPRGVLGLNAPMFKSYLRFICNRRCQQIGLDQLFPNEENPFPWMSEMIDLKKERNFFETRVIEYQTGGALSWD
>Mature_394_residues
MLSWDDDVQATPQAAPQPALQPAAAVTADQQGVLPSSATQAGILGNNPNAAAAQSNRRVNAADKRVINGSTDVNQLVPFK
YKWAWEKYLAGCANHWMPQEINMSRDIATWKDPNGLTEDERRIIKRNLGFFVTADSLAANNIVLGTYRQITAPECRQYLL
RQAFEEAIHTHAYQYIVESLGLNEAEIFNAYHEVQSIRDKDEFLIPFIDTLTDPSFKTGTPENDQKLLKSLIVFACIMEG
LFFYVGFTQILAMGRQNKMTGAAEQYQYILRDESLHCNFGIDLINQIKLENPHLWTAEFKAEITELFKKAVDLEYRYAED
TMPRGVLGLNAPMFKSYLRFICNRRCQQIGLDQLFPNEENPFPWMSEMIDLKKERNFFETRVIEYQTGGALSWD

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Homo sapiens, GI289177074, Length=356, Percent_Identity=25.8426966292135, Blast_Score=138, Evalue=9e-33,
Organism=Homo sapiens, GI42544136, Length=356, Percent_Identity=24.7191011235955, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI260064013, Length=320, Percent_Identity=26.875, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI4557845, Length=320, Percent_Identity=26.875, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI289177076, Length=296, Percent_Identity=26.6891891891892, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI1788567, Length=292, Percent_Identity=26.3698630136986, Blast_Score=101, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17551968, Length=319, Percent_Identity=26.9592476489028, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI193206379, Length=399, Percent_Identity=21.3032581453634, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17567153, Length=224, Percent_Identity=25.4464285714286, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322434, Length=330, Percent_Identity=30.3030303030303, Blast_Score=152, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6321619, Length=320, Percent_Identity=22.5, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI18079279, Length=371, Percent_Identity=24.5283018867925, Blast_Score=141, Evalue=8e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 44947; Mature: 44947

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSWDDDVQATPQAAPQPALQPAAAVTADQQGVLPSSATQAGILGNNPNAAAAQSNRRVN
CCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCC
AADKRVINGSTDVNQLVPFKYKWAWEKYLAGCANHWMPQEINMSRDIATWKDPNGLTEDE
HHHHHEECCCCCHHHHCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHH
RRIIKRNLGFFVTADSLAANNIVLGTYRQITAPECRQYLLRQAFEEAIHTHAYQYIVESL
HHHHHHCCCEEEEECCCCCCCEEEECCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLNEAEIFNAYHEVQSIRDKDEFLIPFIDTLTDPSFKTGTPENDQKLLKSLIVFACIMEG
CCCHHHHHHHHHHHHHHCCCCCEEHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LFFYVGFTQILAMGRQNKMTGAAEQYQYILRDESLHCNFGIDLINQIKLENPHLWTAEFK
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEECCCHHHHHHHCCCCCCEEHHHHH
AEITELFKKAVDLEYRYAEDTMPRGVLGLNAPMFKSYLRFICNRRCQQIGLDQLFPNEEN
HHHHHHHHHHHCCCEEECCCCCCCCEECCCCHHHHHHHHHHHHHHHHHCCHHHHCCCCCC
PFPWMSEMIDLKKERNFFETRVIEYQTGGALSWD
CCCHHHHHHHHHHHCCHHHHHEEEEECCCCCCCC
>Mature Secondary Structure
MLSWDDDVQATPQAAPQPALQPAAAVTADQQGVLPSSATQAGILGNNPNAAAAQSNRRVN
CCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCC
AADKRVINGSTDVNQLVPFKYKWAWEKYLAGCANHWMPQEINMSRDIATWKDPNGLTEDE
HHHHHEECCCCCHHHHCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHH
RRIIKRNLGFFVTADSLAANNIVLGTYRQITAPECRQYLLRQAFEEAIHTHAYQYIVESL
HHHHHHCCCEEEEECCCCCCCEEEECCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLNEAEIFNAYHEVQSIRDKDEFLIPFIDTLTDPSFKTGTPENDQKLLKSLIVFACIMEG
CCCHHHHHHHHHHHHHHCCCCCEEHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LFFYVGFTQILAMGRQNKMTGAAEQYQYILRDESLHCNFGIDLINQIKLENPHLWTAEFK
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEECCCHHHHHHHCCCCCCEEHHHHH
AEITELFKKAVDLEYRYAEDTMPRGVLGLNAPMFKSYLRFICNRRCQQIGLDQLFPNEEN
HHHHHHHHHHHCCCEEECCCCCCCCEECCCCHHHHHHHHHHHHHHHHHCCHHHHCCCCCC
PFPWMSEMIDLKKERNFFETRVIEYQTGGALSWD
CCCHHHHHHHHHHHCCHHHHHEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10684935 [H]