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The map label for this gene is rsmE [H]

Identifier: 73542528

GI number: 73542528

Start: 3116317

End: 3117066

Strand: Direct

Name: rsmE [H]

Synonym: Reut_A2843

Alternate gene names: 73542528

Gene position: 3116317-3117066 (Clockwise)

Preceding gene: 73542527

Following gene: 73542529

Centisome position: 81.87

GC content: 68.4

Gene sequence:

>750_bases
ATGCCACCGCGTTTCTTTGTCGGCAGCGCCGACGCGGCGCTTGCCGCCGGTACCGATTTCGACCTCCCCGAAGCCGTCGT
TCGCCATGTGCAGGTGCTGCGCCTGGCGCCGGGCGACGCCATCACGCTGTTCGATGGCCGTGGCGGCAGCCATGCCGCCA
CGCTGCTTGATTTGGGCAAGCGCCACGCACTCGCGCGCGTGGGTGAGCGAGACAACACGGAAACGGAACCACCATTCCGT
GTCATGCTTGCCCAGGGCCTGGCCGGCGGCGACAAGATGGACTGGCTGATCGAAAAGGCCGTCGAACTGGGCGTGACTGC
CATCCAGCCGCTGCAGGCCGCGCGTTCGGTCGTACGGCTCAGCGGCGACCGCGCGCAGAAGCGCCAGGCCCACTGGCAGG
CGCTCGTAGAAGCTGCCTGCGAGCAATGCGGCCGCAATCGCTTGCCGGCCGTCGCGCCGGTAACTAACTTGGATACGTGG
CTGCGTGCCGCAGGCGAAGGCAACCGGCTCTTGGTGTCGCCACGTGCCGACCAGTCGCTGCCGGCCTATGCCGCGGCGAA
CAGGGTAAGCCTGCTGGCTGGCGGCGTCACGCTGCTGATTGGGCCAGAAGGCGGCCTGGCGCCGGAAGAGGAAGATGCCG
CCCTGCACGCGGGCTTCACCGCCGTGTCACTCGGCCCGCGCATCCTGCGCACCGAAACTGCCGGGCTTGCCTGCCTGGCA
ACGCTGAACGCCTTGCTGGGCGGATTCTGA

Upstream 100 bases:

>100_bases
AGTTCTGGGGCGACCGCTACTGCATGGTCGAAGACCCGGACGGCTACCGCTGGGGTTTCGGCAAACCCGTAGCGGGCAAA
CAGGAAGACAAGACCAGCTG

Downstream 100 bases:

>100_bases
TGGCGGCGCACGCCATCCTTCATCACAAAGGAGTCGACCATGGGATTACTCGATAGCGTGCTGGGCGGGGTGCTCGGGCA
ACTCGGCGGCGCGCGCCAGG

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MPPRFFVGSADAALAAGTDFDLPEAVVRHVQVLRLAPGDAITLFDGRGGSHAATLLDLGKRHALARVGERDNTETEPPFR
VMLAQGLAGGDKMDWLIEKAVELGVTAIQPLQAARSVVRLSGDRAQKRQAHWQALVEAACEQCGRNRLPAVAPVTNLDTW
LRAAGEGNRLLVSPRADQSLPAYAAANRVSLLAGGVTLLIGPEGGLAPEEEDAALHAGFTAVSLGPRILRTETAGLACLA
TLNALLGGF

Sequences:

>Translated_249_residues
MPPRFFVGSADAALAAGTDFDLPEAVVRHVQVLRLAPGDAITLFDGRGGSHAATLLDLGKRHALARVGERDNTETEPPFR
VMLAQGLAGGDKMDWLIEKAVELGVTAIQPLQAARSVVRLSGDRAQKRQAHWQALVEAACEQCGRNRLPAVAPVTNLDTW
LRAAGEGNRLLVSPRADQSLPAYAAANRVSLLAGGVTLLIGPEGGLAPEEEDAALHAGFTAVSLGPRILRTETAGLACLA
TLNALLGGF
>Mature_248_residues
PPRFFVGSADAALAAGTDFDLPEAVVRHVQVLRLAPGDAITLFDGRGGSHAATLLDLGKRHALARVGERDNTETEPPFRV
MLAQGLAGGDKMDWLIEKAVELGVTAIQPLQAARSVVRLSGDRAQKRQAHWQALVEAACEQCGRNRLPAVAPVTNLDTWL
RAAGEGNRLLVSPRADQSLPAYAAANRVSLLAGGVTLLIGPEGGLAPEEEDAALHAGFTAVSLGPRILRTETAGLACLAT
LNALLGGF

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=237, Percent_Identity=37.5527426160338, Blast_Score=160, Evalue=5e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 26087; Mature: 25956

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPRFFVGSADAALAAGTDFDLPEAVVRHVQVLRLAPGDAITLFDGRGGSHAATLLDLGK
CCCCEEECCCCCCEECCCCCCCHHHHHHHHHHHEECCCCEEEEEECCCCCHHHHHHHHHH
RHALARVGERDNTETEPPFRVMLAQGLAGGDKMDWLIEKAVELGVTAIQPLQAARSVVRL
HHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
SGDRAQKRQAHWQALVEAACEQCGRNRLPAVAPVTNLDTWLRAAGEGNRLLVSPRADQSL
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCEEEECCCCCCCC
PAYAAANRVSLLAGGVTLLIGPEGGLAPEEEDAALHAGFTAVSLGPRILRTETAGLACLA
CHHHHHHHHHEEECCEEEEECCCCCCCCCCCCCHHHCCCHHHHCCCHHEECCCCHHHHHH
TLNALLGGF
HHHHHHCCC
>Mature Secondary Structure 
PPRFFVGSADAALAAGTDFDLPEAVVRHVQVLRLAPGDAITLFDGRGGSHAATLLDLGK
CCCEEECCCCCCEECCCCCCCHHHHHHHHHHHEECCCCEEEEEECCCCCHHHHHHHHHH
RHALARVGERDNTETEPPFRVMLAQGLAGGDKMDWLIEKAVELGVTAIQPLQAARSVVRL
HHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
SGDRAQKRQAHWQALVEAACEQCGRNRLPAVAPVTNLDTWLRAAGEGNRLLVSPRADQSL
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCEEEECCCCCCCC
PAYAAANRVSLLAGGVTLLIGPEGGLAPEEEDAALHAGFTAVSLGPRILRTETAGLACLA
CHHHHHHHHHEEECCEEEEECCCCCCCCCCCCCHHHCCCHHHHCCCHHEECCCCHHHHHH
TLNALLGGF
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8332061 [H]