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The map label for this gene is ywnE [H]

Identifier: 73542395

GI number: 73542395

Start: 2974973

End: 2976442

Strand: Direct

Name: ywnE [H]

Synonym: Reut_A2710

Alternate gene names: 73542395

Gene position: 2974973-2976442 (Clockwise)

Preceding gene: 73542394

Following gene: 73542407

Centisome position: 78.15

GC content: 65.1

Gene sequence:

>1470_bases
ATGCCGCTTCCACCGTTCCTGTCCTCGCTGCCCGATCCTGCCCTGTGGTGGGGCCCGCTGTTCACGGTGACCGAATGGGC
GATCCGCCTGACGATGCTGGTCTACGTGCCGCAGCGCCGTGCGCCGGCTGCCGCGCGCACGTGGCTGCTGCTGGTGTTCT
TCCTGCCGTGGCTGGGCCTGATACTTTATGCGCTGTTCGGACGCATCTACTATCCGAAGTCACGCCGCCTGAAACTGGCC
CGCATCAACCAGGGGATCGAGCAGCTGCGCGCGCGCATCAGCCATGAACTGCCACAGGGTCCATTGCCGGATGGGCTGTG
CCAGACGGGTTCCTGCCTGATTGAACGGGTCGGCTCATTCGCGCCGATGCGCGGCAACGATGTCACGCTGATCGACCGCT
ACGACGACGTGATTGCCGCAATGGTGGCCGACATCGACGCCGCGAAGCAGTCGGTGCACATCCTGATGTACATCTACGCG
GACGATGCGACGGGCCGAAGGATTACCGACGCCGTGCTGCGCGCCGCGCGCCGTGGCGTACGCTGCAGCGTGCTGATGGA
CGCGCTCGGTGCGCGCGACGGACTGAAGGCCTTTACCGGCAAGCTGCGCTCTGCCGGCGTGCAGGTGGTACCGGTGCTGC
CGATGCGCTCGCTGTCGGCAGTGCTCAAGGGCAAGAGTGCGCGCTTCGACCTGCGCAATCACCGCAAGATCGTCGTGCTC
GATGGCAGCGTCGGCTATATCGGCTCGCAGAACGTCGTCGATGCCGAATCGATGCCGGGTCTGCCCAATGAGGAACTGGT
GGCCCGCGTGACCGGCCCGGTGGTATCGCAACTGCAGGCTGTGTTCCTCGCGGACCGCTACCTCGAGACCGGCGACAAGG
ACCTCGGCGCTGCCGATTTCCCGCCGCTGGCGCCGGACGGGCCGTGCATTGCGCAGATGCTCCCGAGCGGGCCTGGCTAC
GGCACGCAGAATGCGCAGATGGTGCTGGTGTCGATGATCCACAATGCCGTGCGCCGCGTGGTCATCACCACGCCTTATTT
CGTGCCCGACGAGTCGTTCCTGCAAGCGCTGCACGTCGCGTGCTATCGCGGCGTGGAGGTGCATCTGGTGCTGTCCCAGC
GCATCGACCAGCGCTTCACGCAGGCCGCGCAGGAAGCGTATTTCGGTGTGCTGCTGGAATCGGGCGTGAAGGTCCACCTG
TACCGGCCCGCGTTCCTGCATGCCAAGCATGTGACCATCGACGACACGGTGGCGATGGTCGGCTCGACGAACATGGACAT
CCGCTCGTTCGCGCTCAATGCGGAATCGGCGCTGGTGATTTACGACAGGGATGTGGTGGCGAAGATGGTGCGCATTCAGC
AGCGCTATCTGGCTGCGAGCGACACCGTGGTACGCGAGAAATGGGCCGCGCGGCCGTTGCGCACGCGGACCTGGCAGAAC
CTTTGCCGGTTGGCGGATTCGTTGTTGTGA

Upstream 100 bases:

>100_bases
CGGGCGCGTCGCCGGCTGCATGCGGTTGGCTGCATGCAGCCGGCTCTGGTATGGTCGTGGGCGATTCCAGTGACCTCCGC
CGCCATACCCGAGCCCCGGC

Downstream 100 bases:

>100_bases
TGGCGAGGGCTGCGGCAAGCGCTGGCCCTCTCCACGGAGTTCGGCTTAACGCCCTTCTACAAACCCCAGCACCGCCGCCA
GCATCTGCTTCAGCGACGGC

Product: cardiolipin synthetase 2

Products: NA

Alternate protein names: CL synthase 2 [H]

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MPLPPFLSSLPDPALWWGPLFTVTEWAIRLTMLVYVPQRRAPAAARTWLLLVFFLPWLGLILYALFGRIYYPKSRRLKLA
RINQGIEQLRARISHELPQGPLPDGLCQTGSCLIERVGSFAPMRGNDVTLIDRYDDVIAAMVADIDAAKQSVHILMYIYA
DDATGRRITDAVLRAARRGVRCSVLMDALGARDGLKAFTGKLRSAGVQVVPVLPMRSLSAVLKGKSARFDLRNHRKIVVL
DGSVGYIGSQNVVDAESMPGLPNEELVARVTGPVVSQLQAVFLADRYLETGDKDLGAADFPPLAPDGPCIAQMLPSGPGY
GTQNAQMVLVSMIHNAVRRVVITTPYFVPDESFLQALHVACYRGVEVHLVLSQRIDQRFTQAAQEAYFGVLLESGVKVHL
YRPAFLHAKHVTIDDTVAMVGSTNMDIRSFALNAESALVIYDRDVVAKMVRIQQRYLAASDTVVREKWAARPLRTRTWQN
LCRLADSLL

Sequences:

>Translated_489_residues
MPLPPFLSSLPDPALWWGPLFTVTEWAIRLTMLVYVPQRRAPAAARTWLLLVFFLPWLGLILYALFGRIYYPKSRRLKLA
RINQGIEQLRARISHELPQGPLPDGLCQTGSCLIERVGSFAPMRGNDVTLIDRYDDVIAAMVADIDAAKQSVHILMYIYA
DDATGRRITDAVLRAARRGVRCSVLMDALGARDGLKAFTGKLRSAGVQVVPVLPMRSLSAVLKGKSARFDLRNHRKIVVL
DGSVGYIGSQNVVDAESMPGLPNEELVARVTGPVVSQLQAVFLADRYLETGDKDLGAADFPPLAPDGPCIAQMLPSGPGY
GTQNAQMVLVSMIHNAVRRVVITTPYFVPDESFLQALHVACYRGVEVHLVLSQRIDQRFTQAAQEAYFGVLLESGVKVHL
YRPAFLHAKHVTIDDTVAMVGSTNMDIRSFALNAESALVIYDRDVVAKMVRIQQRYLAASDTVVREKWAARPLRTRTWQN
LCRLADSLL
>Mature_488_residues
PLPPFLSSLPDPALWWGPLFTVTEWAIRLTMLVYVPQRRAPAAARTWLLLVFFLPWLGLILYALFGRIYYPKSRRLKLAR
INQGIEQLRARISHELPQGPLPDGLCQTGSCLIERVGSFAPMRGNDVTLIDRYDDVIAAMVADIDAAKQSVHILMYIYAD
DATGRRITDAVLRAARRGVRCSVLMDALGARDGLKAFTGKLRSAGVQVVPVLPMRSLSAVLKGKSARFDLRNHRKIVVLD
GSVGYIGSQNVVDAESMPGLPNEELVARVTGPVVSQLQAVFLADRYLETGDKDLGAADFPPLAPDGPCIAQMLPSGPGYG
TQNAQMVLVSMIHNAVRRVVITTPYFVPDESFLQALHVACYRGVEVHLVLSQRIDQRFTQAAQEAYFGVLLESGVKVHLY
RPAFLHAKHVTIDDTVAMVGSTNMDIRSFALNAESALVIYDRDVVAKMVRIQQRYLAASDTVVREKWAARPLRTRTWQNL
CRLADSLL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=465, Percent_Identity=29.4623655913978, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI1787007, Length=364, Percent_Identity=28.5714285714286, Blast_Score=125, Evalue=7e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR015679
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 54172; Mature: 54041

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLPPFLSSLPDPALWWGPLFTVTEWAIRLTMLVYVPQRRAPAAARTWLLLVFFLPWLGL
CCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHH
ILYALFGRIYYPKSRRLKLARINQGIEQLRARISHELPQGPLPDGLCQTGSCLIERVGSF
HHHHHHHHEECCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCC
APMRGNDVTLIDRYDDVIAAMVADIDAAKQSVHILMYIYADDATGRRITDAVLRAARRGV
CCCCCCCEEEEECHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHCCC
RCSVLMDALGARDGLKAFTGKLRSAGVQVVPVLPMRSLSAVLKGKSARFDLRNHRKIVVL
CHHHHHHHHCCCCHHHHHHHHHHHCCEEEEECCCHHHHHHHHCCCCCCEECCCCCEEEEE
DGSVGYIGSQNVVDAESMPGLPNEELVARVTGPVVSQLQAVFLADRYLETGDKDLGAADF
ECCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PPLAPDGPCIAQMLPSGPGYGTQNAQMVLVSMIHNAVRRVVITTPYFVPDESFLQALHVA
CCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHH
CYRGVEVHLVLSQRIDQRFTQAAQEAYFGVLLESGVKVHLYRPAFLHAKHVTIDDTVAMV
HHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHEEEEEEECCHHHHH
GSTNMDIRSFALNAESALVIYDRDVVAKMVRIQQRYLAASDTVVREKWAARPLRTRTWQN
CCCCCCHHHEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LCRLADSLL
HHHHHHHCC
>Mature Secondary Structure 
PLPPFLSSLPDPALWWGPLFTVTEWAIRLTMLVYVPQRRAPAAARTWLLLVFFLPWLGL
CCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHH
ILYALFGRIYYPKSRRLKLARINQGIEQLRARISHELPQGPLPDGLCQTGSCLIERVGSF
HHHHHHHHEECCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCC
APMRGNDVTLIDRYDDVIAAMVADIDAAKQSVHILMYIYADDATGRRITDAVLRAARRGV
CCCCCCCEEEEECHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHCCC
RCSVLMDALGARDGLKAFTGKLRSAGVQVVPVLPMRSLSAVLKGKSARFDLRNHRKIVVL
CHHHHHHHHCCCCHHHHHHHHHHHCCEEEEECCCHHHHHHHHCCCCCCEECCCCCEEEEE
DGSVGYIGSQNVVDAESMPGLPNEELVARVTGPVVSQLQAVFLADRYLETGDKDLGAADF
ECCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PPLAPDGPCIAQMLPSGPGYGTQNAQMVLVSMIHNAVRRVVITTPYFVPDESFLQALHVA
CCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHH
CYRGVEVHLVLSQRIDQRFTQAAQEAYFGVLLESGVKVHLYRPAFLHAKHVTIDDTVAMV
HHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHEEEEEEECCHHHHH
GSTNMDIRSFALNAESALVIYDRDVVAKMVRIQQRYLAASDTVVREKWAARPLRTRTWQN
CCCCCCHHHEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LCRLADSLL
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9150240; 9384377 [H]