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The map label for this gene is cysB [H]

Identifier: 73542383

GI number: 73542383

Start: 2960716

End: 2961657

Strand: Direct

Name: cysB [H]

Synonym: Reut_A2698

Alternate gene names: 73542383

Gene position: 2960716-2961657 (Clockwise)

Preceding gene: 73542375

Following gene: 73542384

Centisome position: 77.78

GC content: 60.3

Gene sequence:

>942_bases
ATGAACCTGCACCAGTTTCGCTTTGTGCGTGAGGCGGTACGCCAGAACTACAATCTGACGGAGGCCGCCAAAGCGCTCTA
CACATCACAACCCGGTGTGTCCAAGGCCATCATCGAGCTGGAGGAAGAGCTCGGCGTCGATATCTTCACGCGCCATGGCA
AGCGCATCCGCAGCCTGACGGAGCCGGGGCGCCGCATCCTGAACTCGGTGGAAAAAATTCTTCAGGAGGTGGAAAGCCTC
AAGCGTGTCGGCATGGACTATGCGGCACAGGACCAGGGCAATTTCACCATTGCGACCACGCACACGCAGGCGCGCTACGC
GCTGCCGCGCGTGATCAGCGAGTTCACCAAGCGCTACCCGAAGGTCCGGCTGTCGATCCAGCAGGGCAATCCTTCGCAGA
TTGCCGACATGCTGCTTCATGACCAGGCGGATATCGGCATCGCCACCGAAGGCATCACCCACGAGAAGACCTTGGTCTCC
CTGCCTGGCTACCAATGGCAGCACGTGGTGATTACGCCACCCGATCACCCGCTACTGGAAAAAAAGCATCTGGCGCTCGA
AGACCTGATGAGCTACCCGCTCATCACCTACGACCCGAATTTTGCCGGGCGGCCCAAGATCAACAAGGCGTTCGAGCTGC
GGCACTTGCAGCCTGACATCATCCTGGAAGCGATCGATGCGGACGTGATCAAGACCTATGTGGAGATCGGCCTCGGCATC
GGTATCGTTGCGGGGCTTGCCTATGACGCTGAGCGCGACCGCAACCTGCGCGGCATTCCCGCCGGGCACCTGTTCGGCAC
CAATGTGACGCATCTGGCCGTCAAGCAGGGCGCCTACCTGCGCAGCTTCGTCTATACCTTCATCGAGTTGTTCTCGCCCA
CGCTGAACCGCAAGCTGGTCGAACAGGCGATGTCGGGCGACCACGAGGCGTACGAACTCTGA

Upstream 100 bases:

>100_bases
ATGCGGGCTGGCCGTTCATTAACAAAGTTCGAGAATCGTAATAAACTGGCCTCATACCTCAAACTAATAAGAAGTTGTTT
GTTTATACGACCCGAGTTAT

Downstream 100 bases:

>100_bases
ACGGGGCGGCTACGCCCTTCCCTCTCTATATAGCAGGACAAAATTCATGAACATTTCCCTGGCACTCGTGTGCCGCGCGC
AGCCGGAGGCCCGCCTGTGA

Product: transcriptional regulator CysB-like protein

Products: NA

Alternate protein names: Cys regulon transcriptional activator [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MNLHQFRFVREAVRQNYNLTEAAKALYTSQPGVSKAIIELEEELGVDIFTRHGKRIRSLTEPGRRILNSVEKILQEVESL
KRVGMDYAAQDQGNFTIATTHTQARYALPRVISEFTKRYPKVRLSIQQGNPSQIADMLLHDQADIGIATEGITHEKTLVS
LPGYQWQHVVITPPDHPLLEKKHLALEDLMSYPLITYDPNFAGRPKINKAFELRHLQPDIILEAIDADVIKTYVEIGLGI
GIVAGLAYDAERDRNLRGIPAGHLFGTNVTHLAVKQGAYLRSFVYTFIELFSPTLNRKLVEQAMSGDHEAYEL

Sequences:

>Translated_313_residues
MNLHQFRFVREAVRQNYNLTEAAKALYTSQPGVSKAIIELEEELGVDIFTRHGKRIRSLTEPGRRILNSVEKILQEVESL
KRVGMDYAAQDQGNFTIATTHTQARYALPRVISEFTKRYPKVRLSIQQGNPSQIADMLLHDQADIGIATEGITHEKTLVS
LPGYQWQHVVITPPDHPLLEKKHLALEDLMSYPLITYDPNFAGRPKINKAFELRHLQPDIILEAIDADVIKTYVEIGLGI
GIVAGLAYDAERDRNLRGIPAGHLFGTNVTHLAVKQGAYLRSFVYTFIELFSPTLNRKLVEQAMSGDHEAYEL
>Mature_313_residues
MNLHQFRFVREAVRQNYNLTEAAKALYTSQPGVSKAIIELEEELGVDIFTRHGKRIRSLTEPGRRILNSVEKILQEVESL
KRVGMDYAAQDQGNFTIATTHTQARYALPRVISEFTKRYPKVRLSIQQGNPSQIADMLLHDQADIGIATEGITHEKTLVS
LPGYQWQHVVITPPDHPLLEKKHLALEDLMSYPLITYDPNFAGRPKINKAFELRHLQPDIILEAIDADVIKTYVEIGLGI
GIVAGLAYDAERDRNLRGIPAGHLFGTNVTHLAVKQGAYLRSFVYTFIELFSPTLNRKLVEQAMSGDHEAYEL

Specific function: This protein is a positive regulator of gene expression for the cysteine regulon. The inducer for cysB is N-acetylserine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787530, Length=305, Percent_Identity=43.6065573770492, Blast_Score=281, Evalue=5e-77,
Organism=Escherichia coli, GI1788296, Length=310, Percent_Identity=44.8387096774194, Blast_Score=278, Evalue=3e-76,
Organism=Escherichia coli, GI157672245, Length=213, Percent_Identity=34.2723004694836, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI145693105, Length=264, Percent_Identity=26.8939393939394, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1788481, Length=248, Percent_Identity=29.8387096774194, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788887, Length=247, Percent_Identity=27.1255060728745, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87082132, Length=300, Percent_Identity=25, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1789204, Length=239, Percent_Identity=27.6150627615063, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1790399, Length=256, Percent_Identity=22.65625, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787601, Length=203, Percent_Identity=28.0788177339901, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787806, Length=173, Percent_Identity=27.1676300578035, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI2367136, Length=239, Percent_Identity=23.4309623430962, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787879, Length=260, Percent_Identity=25, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35353; Mature: 35353

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLHQFRFVREAVRQNYNLTEAAKALYTSQPGVSKAIIELEEELGVDIFTRHGKRIRSLT
CCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHCCHHHHHHH
EPGRRILNSVEKILQEVESLKRVGMDYAAQDQGNFTIATTHTQARYALPRVISEFTKRYP
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCC
KVRLSIQQGNPSQIADMLLHDQADIGIATEGITHEKTLVSLPGYQWQHVVITPPDHPLLE
EEEEEEECCCHHHHHHHHHHCCCCCCEECCCCCCCCEEEECCCCCEEEEEECCCCCCCHH
KKHLALEDLMSYPLITYDPNFAGRPKINKAFELRHLQPDIILEAIDADVIKTYVEIGLGI
HHHHHHHHHHHCCEEEECCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
GIVAGLAYDAERDRNLRGIPAGHLFGTNVTHLAVKQGAYLRSFVYTFIELFSPTLNRKLV
HHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHH
EQAMSGDHEAYEL
HHHHCCCCHHHCC
>Mature Secondary Structure
MNLHQFRFVREAVRQNYNLTEAAKALYTSQPGVSKAIIELEEELGVDIFTRHGKRIRSLT
CCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHCCHHHHHHH
EPGRRILNSVEKILQEVESLKRVGMDYAAQDQGNFTIATTHTQARYALPRVISEFTKRYP
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCC
KVRLSIQQGNPSQIADMLLHDQADIGIATEGITHEKTLVSLPGYQWQHVVITPPDHPLLE
EEEEEEECCCHHHHHHHHHHCCCCCCEECCCCCCCCEEEECCCCCEEEEEECCCCCCCHH
KKHLALEDLMSYPLITYDPNFAGRPKINKAFELRHLQPDIILEAIDADVIKTYVEIGLGI
HHHHHHHHHHHCCEEEECCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
GIVAGLAYDAERDRNLRGIPAGHLFGTNVTHLAVKQGAYLRSFVYTFIELFSPTLNRKLV
HHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHH
EQAMSGDHEAYEL
HHHHCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]