Click here to switch to the map view.

The map label for this gene is dctD [H]

Identifier: 73542354

GI number: 73542354

Start: 2932364

End: 2933683

Strand: Direct

Name: dctD [H]

Synonym: Reut_A2669

Alternate gene names: 73542354

Gene position: 2932364-2933683 (Clockwise)

Preceding gene: 73542353

Following gene: 73542355

Centisome position: 77.04

GC content: 68.94

Gene sequence:

>1320_bases
ATGCCGGCTGAACTGACTGTACTGATCGTCGAAGACGACGCCGACGTGCGTCTGGGCTGCGAGCAGGCACTGCGGCTCGA
AGGCATCGCCACGCAAGGCGTGGCAAGTGCCGAGGCGGCCCTGCGCGAGATCACGCCGGGCTGGCCCGGCATCGTAGTCA
GCGACATCCGCCTGCCGGGCATCGATGGCATGGCCTTGCAGGCGGAACTGCGCGGGCGGGATCCGTCGCTCCCGGTGATC
ATGATCACCGGCCACGGCGATGTGAGCCTGGCCGTGCAGGCCATGAAGCAAGGCGCCTACGATTTCCTCGAAAAGCCGTT
CTCGCCCGAGCAGCTCGTCGACACCACGCGCCGCGCGCTCGAGCAGCGCCGTCTGACGCTTGAAGTCGAGGAACTGCGCG
AGCGCCTGGCCGGCCGCGACAAGCTCGCGGCGCAGCTGATCGGCCGCTCGCCGGCGATCGAACGCCTGCGCCGGCTGATT
GCCGATCTGGCCGACACGGCCGCCAACGTGCTGATCCATGGCGAGACCGGTACCGGCAAGGAACTGGTCGCACGCTGCCT
GCACGATGCCAGCCGGCGCCATGCGCGCAATTTCGTCGCCATCAACTGCGGCGGCCTCCCCGAACAGCTGTTCGAGAGCG
AGATCTTCGGCCATGAGGCGGGCTCATTCACCGGCGCCGCGCGCCGCCGCATCGGCAAGGTCGAGCACGCCGAAGGCGGC
ACGCTGTTCCTCGACGAAATCGAGAGCATGCCGATGCCGCTGCAGATCAAGCTGCTGCGGGTCCTGCAGGAACGCGTGGT
GGAACGCCTGGGGTCGAACGAGCCCGTGCCCGTGGACACGCGTGTGGTCGCGGCCACCAAGGCCGACCTGCGCGCGTTGT
CCGACGCAGGGCAGTTTCGCTCGGACCTTTATTACCGCCTCAACGTCATCACGCTGGAACTGCCGGCGCTACGCGACCGG
CGCGAAGACGTGCCGCTGCTGTTTGAGCATTTCGTGGCGCAGGCCGCGCTGCGCTTCGGGCGCGACGCCGTGCCGGCCAC
GCCCGCCGAACTGGCCACGCTGGTCGCCTATCCCTGGCCCGGCAATGTGCGGGAACTGCGCAACCTGGCCGAACGCCACG
TGCTCGGTCTCGGCTGCGATCCGGGCCACGGCACCTCCGCGCCGGAAGCACTGCCACTGGCGCAGGCGGTCGAGCAATTC
GAGCGCGCGCTGATTGCCGACGCGCTGCGCCGGCACGACGGCAACCTCAGCCGCGCCAGCGAGGCGCTGGGCGTGGCCAA
GACTACGCTGTTCGACAAGACCCGCAAGTACGGCCTGTAG

Upstream 100 bases:

>100_bases
ATCCTCAACGAGAACGGTGGCAGCCTGTCCGCGTCCAATCACCCCGACGGCGGCGCGTGCTTCACGGTGGTCCTGCCCTT
GGTCCGCCAGGAAATCCCAC

Downstream 100 bases:

>100_bases
CCGGCACCCGCTCCACACCGGGGGAACTGCTTGCGATGGCGGAGGTCTGCTGTGCAAGCACGACCCACGATTTTGGTCGA
GAGCCGCGAATTGCAAGAAT

Product: two component, sigma54 specific, Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MPAELTVLIVEDDADVRLGCEQALRLEGIATQGVASAEAALREITPGWPGIVVSDIRLPGIDGMALQAELRGRDPSLPVI
MITGHGDVSLAVQAMKQGAYDFLEKPFSPEQLVDTTRRALEQRRLTLEVEELRERLAGRDKLAAQLIGRSPAIERLRRLI
ADLADTAANVLIHGETGTGKELVARCLHDASRRHARNFVAINCGGLPEQLFESEIFGHEAGSFTGAARRRIGKVEHAEGG
TLFLDEIESMPMPLQIKLLRVLQERVVERLGSNEPVPVDTRVVAATKADLRALSDAGQFRSDLYYRLNVITLELPALRDR
REDVPLLFEHFVAQAALRFGRDAVPATPAELATLVAYPWPGNVRELRNLAERHVLGLGCDPGHGTSAPEALPLAQAVEQF
ERALIADALRRHDGNLSRASEALGVAKTTLFDKTRKYGL

Sequences:

>Translated_439_residues
MPAELTVLIVEDDADVRLGCEQALRLEGIATQGVASAEAALREITPGWPGIVVSDIRLPGIDGMALQAELRGRDPSLPVI
MITGHGDVSLAVQAMKQGAYDFLEKPFSPEQLVDTTRRALEQRRLTLEVEELRERLAGRDKLAAQLIGRSPAIERLRRLI
ADLADTAANVLIHGETGTGKELVARCLHDASRRHARNFVAINCGGLPEQLFESEIFGHEAGSFTGAARRRIGKVEHAEGG
TLFLDEIESMPMPLQIKLLRVLQERVVERLGSNEPVPVDTRVVAATKADLRALSDAGQFRSDLYYRLNVITLELPALRDR
REDVPLLFEHFVAQAALRFGRDAVPATPAELATLVAYPWPGNVRELRNLAERHVLGLGCDPGHGTSAPEALPLAQAVEQF
ERALIADALRRHDGNLSRASEALGVAKTTLFDKTRKYGL
>Mature_438_residues
PAELTVLIVEDDADVRLGCEQALRLEGIATQGVASAEAALREITPGWPGIVVSDIRLPGIDGMALQAELRGRDPSLPVIM
ITGHGDVSLAVQAMKQGAYDFLEKPFSPEQLVDTTRRALEQRRLTLEVEELRERLAGRDKLAAQLIGRSPAIERLRRLIA
DLADTAANVLIHGETGTGKELVARCLHDASRRHARNFVAINCGGLPEQLFESEIFGHEAGSFTGAARRRIGKVEHAEGGT
LFLDEIESMPMPLQIKLLRVLQERVVERLGSNEPVPVDTRVVAATKADLRALSDAGQFRSDLYYRLNVITLELPALRDRR
EDVPLLFEHFVAQAALRFGRDAVPATPAELATLVAYPWPGNVRELRNLAERHVLGLGCDPGHGTSAPEALPLAQAVEQFE
RALIADALRRHDGNLSRASEALGVAKTTLFDKTRKYGL

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. When activated by dctB acts in conjunction with sigma-54 to activate the transcription of dctA [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=457, Percent_Identity=39.3873085339169, Blast_Score=331, Evalue=7e-92,
Organism=Escherichia coli, GI1790437, Length=440, Percent_Identity=40, Blast_Score=307, Evalue=1e-84,
Organism=Escherichia coli, GI1790299, Length=468, Percent_Identity=38.034188034188, Blast_Score=301, Evalue=6e-83,
Organism=Escherichia coli, GI1788905, Length=450, Percent_Identity=39.7777777777778, Blast_Score=294, Evalue=1e-80,
Organism=Escherichia coli, GI1789087, Length=332, Percent_Identity=43.9759036144578, Blast_Score=245, Evalue=5e-66,
Organism=Escherichia coli, GI87082117, Length=343, Percent_Identity=42.8571428571429, Blast_Score=240, Evalue=1e-64,
Organism=Escherichia coli, GI1789233, Length=320, Percent_Identity=40.3125, Blast_Score=221, Evalue=7e-59,
Organism=Escherichia coli, GI1786524, Length=356, Percent_Identity=39.8876404494382, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI87082152, Length=231, Percent_Identity=48.9177489177489, Blast_Score=204, Evalue=8e-54,
Organism=Escherichia coli, GI87081872, Length=306, Percent_Identity=39.8692810457516, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI1787583, Length=334, Percent_Identity=37.4251497005988, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI87081858, Length=368, Percent_Identity=29.0760869565217, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1789828, Length=243, Percent_Identity=37.037037037037, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1789809, Length=123, Percent_Identity=31.7073170731707, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1790552, Length=108, Percent_Identity=34.2592592592593, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1786599, Length=130, Percent_Identity=30, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 48020; Mature: 47888

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4 ; PS00758 ARGE_DAPE_CPG2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAELTVLIVEDDADVRLGCEQALRLEGIATQGVASAEAALREITPGWPGIVVSDIRLPG
CCCCEEEEEEECCCCCHHCHHHHHHHHCCHHCCHHHHHHHHHHCCCCCCCEEEECEECCC
IDGMALQAELRGRDPSLPVIMITGHGDVSLAVQAMKQGAYDFLEKPFSPEQLVDTTRRAL
CCCCEEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHHHH
EQRRLTLEVEELRERLAGRDKLAAQLIGRSPAIERLRRLIADLADTAANVLIHGETGTGK
HHHHCEEEHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEECCCCCCH
ELVARCLHDASRRHARNFVAINCGGLPEQLFESEIFGHEAGSFTGAARRRIGKVEHAEGG
HHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCC
TLFLDEIESMPMPLQIKLLRVLQERVVERLGSNEPVPVDTRVVAATKADLRALSDAGQFR
EEEHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHHHHH
SDLYYRLNVITLELPALRDRREDVPLLFEHFVAQAALRFGRDAVPATPAELATLVAYPWP
HHHEEEEEEEEEECCHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHEECCCC
GNVRELRNLAERHVLGLGCDPGHGTSAPEALPLAQAVEQFERALIADALRRHDGNLSRAS
CCHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
EALGVAKTTLFDKTRKYGL
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PAELTVLIVEDDADVRLGCEQALRLEGIATQGVASAEAALREITPGWPGIVVSDIRLPG
CCCEEEEEEECCCCCHHCHHHHHHHHCCHHCCHHHHHHHHHHCCCCCCCEEEECEECCC
IDGMALQAELRGRDPSLPVIMITGHGDVSLAVQAMKQGAYDFLEKPFSPEQLVDTTRRAL
CCCCEEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHHHH
EQRRLTLEVEELRERLAGRDKLAAQLIGRSPAIERLRRLIADLADTAANVLIHGETGTGK
HHHHCEEEHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEECCCCCCH
ELVARCLHDASRRHARNFVAINCGGLPEQLFESEIFGHEAGSFTGAARRRIGKVEHAEGG
HHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCC
TLFLDEIESMPMPLQIKLLRVLQERVVERLGSNEPVPVDTRVVAATKADLRALSDAGQFR
EEEHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHHHHH
SDLYYRLNVITLELPALRDRREDVPLLFEHFVAQAALRFGRDAVPATPAELATLVAYPWP
HHHEEEEEEEEEECCHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHEECCCC
GNVRELRNLAERHVLGLGCDPGHGTSAPEALPLAQAVEQFERALIADALRRHDGNLSRAS
CCHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
EALGVAKTTLFDKTRKYGL
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2134335; 2793824; 11481431 [H]