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The map label for this gene is mltF [H]

Identifier: 73542344

GI number: 73542344

Start: 2917288

End: 2918814

Strand: Direct

Name: mltF [H]

Synonym: Reut_A2659

Alternate gene names: 73542344

Gene position: 2917288-2918814 (Clockwise)

Preceding gene: 73542343

Following gene: 73542348

Centisome position: 76.64

GC content: 62.93

Gene sequence:

>1527_bases
ATGCCCGCTTTCCGCTTGCCGCCCACGCTGCCAGGCCTCCTTGTGCAAGGCCTGGTGGGCGCTGCATGCCTCTTCGGCCC
GATTGCCGCCGCGCAGCAAAAGCCCACTCCCGCCTCGGCACCCGTGGCTGTCGCCCCGGCCAAAGCAGATTCCGCAGCCG
TACGCCGGCTCAACCTGGGCAACAAACCCTGGAAAGGCGATTTCGACGCGATGCTCGATCGCCGCGTCATCCGCGTCCTG
GTGCCATACAGCCGTACGCTGTATTTCAGCGACAAGGGCCGTGAATGCGGCCTGACCGCCGGACTGGTGCGGGACTTCGA
GCGCTATCTGAACAAGACCTACGCCAGCCGCCTCGGCAAGCGGCCGCTCACGGTCATCGTCATTCCCACGACGCGTGACC
GCCTGCTGCCCGACCTGGCCGCCGGACTGGGCGACATTGCGGCCGGCAACCTGACAGCGACCGACGAACGTCTGAAGGTT
GCCGACTTCTCGGCGCCGCGCGACCGCAAGCCGGTGCGCGAACTGATCGTGACCGGTTCGAAGGCGCCTGCCCTGGCCAG
TCTGGACGACTTGTCCGGCAAGACGGTGCATGTACGGCGCGCCAGCAGCTACTACGAAAGCCTCACGGCGCTCAATGACC
AGTTGCGCAAGGCCGGCAAGGCACCCATGCGCCTGGTGCTGCTGCCCGATGCGCTGGAAGACGAAGACGCGATGGAGATG
GCCAATGCCGGCTTGCTGCCCATCCTCGTGGTGGATGACTGGAAGGCCGCGATGTGGGCGCAGATCCTGCCCAACATCAA
GGTACACAATGACCTCGTCGTCCGCGCGCAAGGCTATGTCGGCTGGGCCTACCGCAAGGACAGCCCGCAGATGCGCCAGG
CGCTGGACGATTTCTACGTGAACTACCTGAAGAAGCAGGGCGTCGCCGAATACCGGCTCAAGCAGATGATGAAGAGTGTC
CGGCAGATCCGGAACAATACCAACGACGCCGAGTACAAGCGCTTCGAGCAGACCATTGCCTTCTTCCAGAAATACGGCAA
GGACTACAGCTTCGATCCGTTGATGCTGGCAGCGCAGGGCTTCCAGGAATCGCAGCTGAACCAGAAGGCGCGCAGCCACG
TGGGCGCCATCGGCATCATGCAGGTCATGCCAGCCACCGGCAAGCAACTCAATGTGGGCAATATCCAGGAGCCCGAATCA
AACATCCACGCTGGCACCAAATACATGGACCAGCTGATGACGCGGTATTTCCCCGATGCGCGCTTCTCGGAATCCAACCG
GCCGCTCTTTGCATTCGCCAGCTATAACGCGGGCCCGGGCAATATCGCGAAGATGCGCACGGAAGCCGCGGCGCGCGGGC
TGGATCCGGACCAGTGGTTCAACAATGTGGAGATTGTCGTGGCCGAGAAGATCGGCATCGAGACCACGACCTATGTGCGC
AACATCTACAAGTACTACGCTGCGTACCGGCTGGCGCAGGACATGGAGGACACGCGCGCACGCGCGCTGAAGGAAGTCGG
TAAATAG

Upstream 100 bases:

>100_bases
ACCTTCGCCGGCGGGCTTACGCACGGCTTGTACCGTGCCGTATCCGCGCTAGACTCAATGAGCATGCGCCGCAGGCAATC
CGGCAGCGGCGCTGCGCGAG

Downstream 100 bases:

>100_bases
CCGGCCGGTCGCGCGCGCATCGCGGTGGTCATGCGTGCCGCCGGACGGTGCTAGTCGGCCAGGCCGCTCGCGTCGATATA
ACCGAGCGCTGCGCTCACCG

Product: lytic transglycosylase, catalytic

Products: 1,6-Anhydrobond [C]

Alternate protein names: Murein lyase F [H]

Number of amino acids: Translated: 508; Mature: 507

Protein sequence:

>508_residues
MPAFRLPPTLPGLLVQGLVGAACLFGPIAAAQQKPTPASAPVAVAPAKADSAAVRRLNLGNKPWKGDFDAMLDRRVIRVL
VPYSRTLYFSDKGRECGLTAGLVRDFERYLNKTYASRLGKRPLTVIVIPTTRDRLLPDLAAGLGDIAAGNLTATDERLKV
ADFSAPRDRKPVRELIVTGSKAPALASLDDLSGKTVHVRRASSYYESLTALNDQLRKAGKAPMRLVLLPDALEDEDAMEM
ANAGLLPILVVDDWKAAMWAQILPNIKVHNDLVVRAQGYVGWAYRKDSPQMRQALDDFYVNYLKKQGVAEYRLKQMMKSV
RQIRNNTNDAEYKRFEQTIAFFQKYGKDYSFDPLMLAAQGFQESQLNQKARSHVGAIGIMQVMPATGKQLNVGNIQEPES
NIHAGTKYMDQLMTRYFPDARFSESNRPLFAFASYNAGPGNIAKMRTEAAARGLDPDQWFNNVEIVVAEKIGIETTTYVR
NIYKYYAAYRLAQDMEDTRARALKEVGK

Sequences:

>Translated_508_residues
MPAFRLPPTLPGLLVQGLVGAACLFGPIAAAQQKPTPASAPVAVAPAKADSAAVRRLNLGNKPWKGDFDAMLDRRVIRVL
VPYSRTLYFSDKGRECGLTAGLVRDFERYLNKTYASRLGKRPLTVIVIPTTRDRLLPDLAAGLGDIAAGNLTATDERLKV
ADFSAPRDRKPVRELIVTGSKAPALASLDDLSGKTVHVRRASSYYESLTALNDQLRKAGKAPMRLVLLPDALEDEDAMEM
ANAGLLPILVVDDWKAAMWAQILPNIKVHNDLVVRAQGYVGWAYRKDSPQMRQALDDFYVNYLKKQGVAEYRLKQMMKSV
RQIRNNTNDAEYKRFEQTIAFFQKYGKDYSFDPLMLAAQGFQESQLNQKARSHVGAIGIMQVMPATGKQLNVGNIQEPES
NIHAGTKYMDQLMTRYFPDARFSESNRPLFAFASYNAGPGNIAKMRTEAAARGLDPDQWFNNVEIVVAEKIGIETTTYVR
NIYKYYAAYRLAQDMEDTRARALKEVGK
>Mature_507_residues
PAFRLPPTLPGLLVQGLVGAACLFGPIAAAQQKPTPASAPVAVAPAKADSAAVRRLNLGNKPWKGDFDAMLDRRVIRVLV
PYSRTLYFSDKGRECGLTAGLVRDFERYLNKTYASRLGKRPLTVIVIPTTRDRLLPDLAAGLGDIAAGNLTATDERLKVA
DFSAPRDRKPVRELIVTGSKAPALASLDDLSGKTVHVRRASSYYESLTALNDQLRKAGKAPMRLVLLPDALEDEDAMEMA
NAGLLPILVVDDWKAAMWAQILPNIKVHNDLVVRAQGYVGWAYRKDSPQMRQALDDFYVNYLKKQGVAEYRLKQMMKSVR
QIRNNTNDAEYKRFEQTIAFFQKYGKDYSFDPLMLAAQGFQESQLNQKARSHVGAIGIMQVMPATGKQLNVGNIQEPESN
IHAGTKYMDQLMTRYFPDARFSESNRPLFAFASYNAGPGNIAKMRTEAAARGLDPDQWFNNVEIVVAEKIGIETTTYVRN
IYKYYAAYRLAQDMEDTRARALKEVGK

Specific function: Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the

COG id: COG4623

COG function: function code M; Predicted soluble lytic transglycosylase fused to an ABC-type amino acid-binding protein

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein. Note=Attached to the inner leaflet of the outer membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI171474010, Length=453, Percent_Identity=23.1788079470199, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI87082441, Length=161, Percent_Identity=31.055900621118, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR001638
- InterPro:   IPR000189 [H]

Pfam domain/function: PF00497 SBP_bac_3; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 56564; Mature: 56432

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAFRLPPTLPGLLVQGLVGAACLFGPIAAAQQKPTPASAPVAVAPAKADSAAVRRLNLG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHCCC
NKPWKGDFDAMLDRRVIRVLVPYSRTLYFSDKGRECGLTAGLVRDFERYLNKTYASRLGK
CCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
RPLTVIVIPTTRDRLLPDLAAGLGDIAAGNLTATDERLKVADFSAPRDRKPVRELIVTGS
CCEEEEEECCCHHHHHHHHHHCCHHHCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHCCC
KAPALASLDDLSGKTVHVRRASSYYESLTALNDQLRKAGKAPMRLVLLPDALEDEDAMEM
CCCCCCCHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHH
ANAGLLPILVVDDWKAAMWAQILPNIKVHNDLVVRAQGYVGWAYRKDSPQMRQALDDFYV
HCCCCEEEEEECCCHHHHHHHHCCCCEECCCEEEEECCCEEEEEECCCHHHHHHHHHHHH
NYLKKQGVAEYRLKQMMKSVRQIRNNTNDAEYKRFEQTIAFFQKYGKDYSFDPLMLAAQG
HHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCC
FQESQLNQKARSHVGAIGIMQVMPATGKQLNVGNIQEPESNIHAGTKYMDQLMTRYFPDA
CHHHHHHHHHHHHHCHHHHHHHCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
RFSESNRPLFAFASYNAGPGNIAKMRTEAAARGLDPDQWFNNVEIVVAEKIGIETTTYVR
CCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHCCCEEEEEEECCCCCHHHHHH
NIYKYYAAYRLAQDMEDTRARALKEVGK
HHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PAFRLPPTLPGLLVQGLVGAACLFGPIAAAQQKPTPASAPVAVAPAKADSAAVRRLNLG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHCCC
NKPWKGDFDAMLDRRVIRVLVPYSRTLYFSDKGRECGLTAGLVRDFERYLNKTYASRLGK
CCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
RPLTVIVIPTTRDRLLPDLAAGLGDIAAGNLTATDERLKVADFSAPRDRKPVRELIVTGS
CCEEEEEECCCHHHHHHHHHHCCHHHCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHCCC
KAPALASLDDLSGKTVHVRRASSYYESLTALNDQLRKAGKAPMRLVLLPDALEDEDAMEM
CCCCCCCHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHH
ANAGLLPILVVDDWKAAMWAQILPNIKVHNDLVVRAQGYVGWAYRKDSPQMRQALDDFYV
HCCCCEEEEEECCCHHHHHHHHCCCCEECCCEEEEECCCEEEEEECCCHHHHHHHHHHHH
NYLKKQGVAEYRLKQMMKSVRQIRNNTNDAEYKRFEQTIAFFQKYGKDYSFDPLMLAAQG
HHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCC
FQESQLNQKARSHVGAIGIMQVMPATGKQLNVGNIQEPESNIHAGTKYMDQLMTRYFPDA
CHHHHHHHHHHHHHCHHHHHHHCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
RFSESNRPLFAFASYNAGPGNIAKMRTEAAARGLDPDQWFNNVEIVVAEKIGIETTTYVR
CCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHCCCEEEEEEECCCCCHHHHHH
NIYKYYAAYRLAQDMEDTRARALKEVGK
HHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA