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The map label for this gene is gcvA [H]

Identifier: 73542316

GI number: 73542316

Start: 2886293

End: 2887243

Strand: Direct

Name: gcvA [H]

Synonym: Reut_A2631

Alternate gene names: 73542316

Gene position: 2886293-2887243 (Clockwise)

Preceding gene: 73542314

Following gene: 73542317

Centisome position: 75.82

GC content: 66.88

Gene sequence:

>951_bases
ATGGCCCGCAAACCTATCGATCGCCCTGTCCACATCCCGCCGTTGCAAGCGCTGCGTGCGCTGGAAGCTGCCGCGCGCCA
TCGCAGCTTCTCGCGTGCCGCAGAAGAGCTTGCACTGACCCACAGCGCCATCAGTCATCACATGCGGGCGCTCGAGGACA
AGCTCGGCACCAAGCTATTTCAGCGTACCGGCAGCCAGATGGCGCCGACCAGTGCCGGAGCAAGGCTCGCCGAGCAAATC
CGTGCCGCGCTGGACGATATTGAAAGCGCCGTGCGCGAAGCCAGCAGCAGCACGACCACACCGGTCGTGCGACTGCAGGT
CAGCGTCATGGCGGACCTGGCCAACGCCTGGCTGATTCGCCGCTTGCCGAGCCTGCACGCCCAGGTGCCGTCGCTGGACC
TGCACCTGCGGCTGCATGCCGAGATCACGCCGCCCGACCCGTACAGCGTCGACGTCGGCATCTGGCACCAGCGCATCGAT
CTTCCCGGCTTCGAATGCCACAACCTGATCGAAGACCATGTTATCGCCGTGGCCAGCCCGGCGCTCCTGGCGCGCTACCC
CGGCTTCACGCCAGCCGACGTGCCTCGCATGCCGATGCTGCGCTTTGCCCTGCGGCCCTGGCGCGACTGGCTCGAAGCCG
CCGGCCTGCCAGATGCCGAGCCCGAACGCGGCCCGATCTTCCAGGACGCCGGGCTGATGCTGCAGTCAGCCGTGGCCGGC
CTGGGCGTAGCCACCGCCAGGGCGCAACTGGCCCACGACTACCTTGAGAGCGGCCAGTTGGTGCAGGTCGGTTCTACCCG
CATTCCGTCCAGCCTGCATTACTGGGTCACCTGGCGCGAGGGCAATCCTCGCGAAAAAGCGATCCAGCAGTTCCATGCCT
GGCTGCAGGAACAGGTGCGCCGCGAAACGCCGACCGCGGAGCCGACGACGACAGACGACGCCATGAAGTAA

Upstream 100 bases:

>100_bases
CGGGTAGCAGCAGAGGTCGTCATGCCGCCATTGTCCTCACCAGCATGGTGCAACGCCAACGAAAGTTACGGTCGGCAATT
GTGAGTAGAATTTGACGGCC

Downstream 100 bases:

>100_bases
CAGACATGAAATGTTTTCGATGTCCAAAGTTGGCAATACGAATCATTTTCGTTTGATTCAGAAGAATCCATGGCTTAGCG
TCATGCAGGGAAGCGTTGCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MARKPIDRPVHIPPLQALRALEAAARHRSFSRAAEELALTHSAISHHMRALEDKLGTKLFQRTGSQMAPTSAGARLAEQI
RAALDDIESAVREASSSTTTPVVRLQVSVMADLANAWLIRRLPSLHAQVPSLDLHLRLHAEITPPDPYSVDVGIWHQRID
LPGFECHNLIEDHVIAVASPALLARYPGFTPADVPRMPMLRFALRPWRDWLEAAGLPDAEPERGPIFQDAGLMLQSAVAG
LGVATARAQLAHDYLESGQLVQVGSTRIPSSLHYWVTWREGNPREKAIQQFHAWLQEQVRRETPTAEPTTTDDAMK

Sequences:

>Translated_316_residues
MARKPIDRPVHIPPLQALRALEAAARHRSFSRAAEELALTHSAISHHMRALEDKLGTKLFQRTGSQMAPTSAGARLAEQI
RAALDDIESAVREASSSTTTPVVRLQVSVMADLANAWLIRRLPSLHAQVPSLDLHLRLHAEITPPDPYSVDVGIWHQRID
LPGFECHNLIEDHVIAVASPALLARYPGFTPADVPRMPMLRFALRPWRDWLEAAGLPDAEPERGPIFQDAGLMLQSAVAG
LGVATARAQLAHDYLESGQLVQVGSTRIPSSLHYWVTWREGNPREKAIQQFHAWLQEQVRRETPTAEPTTTDDAMK
>Mature_315_residues
ARKPIDRPVHIPPLQALRALEAAARHRSFSRAAEELALTHSAISHHMRALEDKLGTKLFQRTGSQMAPTSAGARLAEQIR
AALDDIESAVREASSSTTTPVVRLQVSVMADLANAWLIRRLPSLHAQVPSLDLHLRLHAEITPPDPYSVDVGIWHQRIDL
PGFECHNLIEDHVIAVASPALLARYPGFTPADVPRMPMLRFALRPWRDWLEAAGLPDAEPERGPIFQDAGLMLQSAVAGL
GVATARAQLAHDYLESGQLVQVGSTRIPSSLHYWVTWREGNPREKAIQQFHAWLQEQVRRETPTAEPTTTDDAMK

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=288, Percent_Identity=32.9861111111111, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1786448, Length=294, Percent_Identity=28.9115646258503, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1788706, Length=310, Percent_Identity=26.4516129032258, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1787128, Length=280, Percent_Identity=27.5, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786401, Length=278, Percent_Identity=23.7410071942446, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787589, Length=137, Percent_Identity=29.1970802919708, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1790399, Length=122, Percent_Identity=31.1475409836066, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35037; Mature: 34906

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKPIDRPVHIPPLQALRALEAAARHRSFSRAAEELALTHSAISHHMRALEDKLGTKLF
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRTGSQMAPTSAGARLAEQIRAALDDIESAVREASSSTTTPVVRLQVSVMADLANAWLIR
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
RLPSLHAQVPSLDLHLRLHAEITPPDPYSVDVGIWHQRIDLPGFECHNLIEDHVIAVASP
HHHHHHHCCCCCEEEEEEEEEECCCCCCEEEHHHHHHHCCCCCHHHHHHHHHHHHHHCCH
ALLARYPGFTPADVPRMPMLRFALRPWRDWLEAAGLPDAEPERGPIFQDAGLMLQSAVAG
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
LGVATARAQLAHDYLESGQLVQVGSTRIPSSLHYWVTWREGNPREKAIQQFHAWLQEQVR
HHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHH
RETPTAEPTTTDDAMK
HCCCCCCCCCCHHCCC
>Mature Secondary Structure 
ARKPIDRPVHIPPLQALRALEAAARHRSFSRAAEELALTHSAISHHMRALEDKLGTKLF
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRTGSQMAPTSAGARLAEQIRAALDDIESAVREASSSTTTPVVRLQVSVMADLANAWLIR
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
RLPSLHAQVPSLDLHLRLHAEITPPDPYSVDVGIWHQRIDLPGFECHNLIEDHVIAVASP
HHHHHHHCCCCCEEEEEEEEEECCCCCCEEEHHHHHHHCCCCCHHHHHHHHHHHHHHCCH
ALLARYPGFTPADVPRMPMLRFALRPWRDWLEAAGLPDAEPERGPIFQDAGLMLQSAVAG
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
LGVATARAQLAHDYLESGQLVQVGSTRIPSSLHYWVTWREGNPREKAIQQFHAWLQEQVR
HHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHH
RETPTAEPTTTDDAMK
HCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]