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The map label for this gene is hemL [H]

Identifier: 73542310

GI number: 73542310

Start: 2880659

End: 2881948

Strand: Direct

Name: hemL [H]

Synonym: Reut_A2625

Alternate gene names: 73542310

Gene position: 2880659-2881948 (Clockwise)

Preceding gene: 73542303

Following gene: 73542311

Centisome position: 75.68

GC content: 68.29

Gene sequence:

>1290_bases
ATGTCCCGTAACCAGCAGCTCTTCGACCGTGCCCAGCAAACCATTCCCGGCGGCGTCAACTCGCCCGTGCGGGCCTTCCG
CTCGGTGGGCGGCACGCCGCGCTTCATCACGTGTGCCGAGGGCGCATACATGTGGGATGCGGATGGCCAGCGCTATATCG
ACTACATCGGCTCCTGGGGCCCGATGATCGTCGGCCACGCCCACCCCGACGTGGTCCGCGCCGTGCAGGAGACCGCCGCG
CACAGCTTCTCGTTCGGCGCCCCGACCGAGGCCGAGATCGACATGGCCGAGGAAATCTGCAAGCTGGTGCCGTCGATCGA
ACAGGTGCGCCTGGTGTCGTCGGGCACCGAGGCAACCATGAGCGCGCTGCGCCTGGCGCGCGGGTTCACCGGCCGCGACC
TGATCATCAAGTTCGAAGGCTGCTACCACGGCCATGCCGACAGCCTGCTGGTCAAGGCCGGCTCGGGCCTGCTGACCTTT
GCCGACACCACGCAGAACGCACCGTCGTCGGCCGGCGTGCCCGCGGACGTGACGCGCCACACCATGGTGCTCGAGTACAA
CAACGTCGCGCAGCTCGAAGAAGCGTTTGCCCAGCACCGCGGCGAGATTGCGGCCGTGATCGTGGAAGTGGTGGCCGGCA
ACATGAACCTCGTGCGCGCGAGCGACGCGTTCCTGAAGGCCATGCGTGAACTGTGCACGCGCGACGGCGCCGTGCTGATC
TTCGACGAAGTCATGACCGGGTTCCGCGTGGCGCTGGGCGGCGCGCAGTCCCACTACGGCATCACGCCGGACATGACGTG
CCTGGGCAAGGTCATCGGTGGCGGCATGCCGGCAGCGGCGTTTGGCGGCCGGCGCGACATCATGGCCAAGCTCGCGCCGC
TGGGTGGCGTGTACCAGGCGGGCACGCTGTCGGGCAATCCGCTGGCCGTGGCGGCCGGTCTGACCACACTGCGGCTGATC
CAGGCACCGGGCTTCTACGACAAACTGACCGCGCAAACCCGCAAGCTGGCCGATGGCCTGAGCGAAGCGGCCCGTGCGGC
CGGCGTGCCGTTCGCGGCCGATGCCATTGGCGGCATGTTCGGCATCTACTTCCGCGACGGCGTGCCAGGCAGCTTTGCCG
AGGTGACGAAGAGCGACACCGCGCGCTTCAACCGCTTCTTCCACGCCATGCTCGACAACGGCGTGTACCTGGCGCCCTCG
GCATTCGAGGCCGGCTTTGTCTCGGCCTGCCATGACGACGCCATCCTGCAGGCCACGCTGGACGCCGCACGCAAGGCCTT
CGCGGCCTGA

Upstream 100 bases:

>100_bases
GCCGCGCTATTCCACCGATTTGCACCGCTTTGCCCACGCTTTGGACGCCAGTTCGCGCCCGCTTGAACGCGTTTCCCCCG
AGTTTTCCACCAGGCAGACC

Downstream 100 bases:

>100_bases
CGGGAGCCAAAGCAGGGACCGCGGCTGTCAGCAACGCGCCGTGCGCTACACTTGAGATTCGCCTCAAACCGGGAGCCCAC
GATGCGCGCCTCACTGCCCC

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSRNQQLFDRAQQTIPGGVNSPVRAFRSVGGTPRFITCAEGAYMWDADGQRYIDYIGSWGPMIVGHAHPDVVRAVQETAA
HSFSFGAPTEAEIDMAEEICKLVPSIEQVRLVSSGTEATMSALRLARGFTGRDLIIKFEGCYHGHADSLLVKAGSGLLTF
ADTTQNAPSSAGVPADVTRHTMVLEYNNVAQLEEAFAQHRGEIAAVIVEVVAGNMNLVRASDAFLKAMRELCTRDGAVLI
FDEVMTGFRVALGGAQSHYGITPDMTCLGKVIGGGMPAAAFGGRRDIMAKLAPLGGVYQAGTLSGNPLAVAAGLTTLRLI
QAPGFYDKLTAQTRKLADGLSEAARAAGVPFAADAIGGMFGIYFRDGVPGSFAEVTKSDTARFNRFFHAMLDNGVYLAPS
AFEAGFVSACHDDAILQATLDAARKAFAA

Sequences:

>Translated_429_residues
MSRNQQLFDRAQQTIPGGVNSPVRAFRSVGGTPRFITCAEGAYMWDADGQRYIDYIGSWGPMIVGHAHPDVVRAVQETAA
HSFSFGAPTEAEIDMAEEICKLVPSIEQVRLVSSGTEATMSALRLARGFTGRDLIIKFEGCYHGHADSLLVKAGSGLLTF
ADTTQNAPSSAGVPADVTRHTMVLEYNNVAQLEEAFAQHRGEIAAVIVEVVAGNMNLVRASDAFLKAMRELCTRDGAVLI
FDEVMTGFRVALGGAQSHYGITPDMTCLGKVIGGGMPAAAFGGRRDIMAKLAPLGGVYQAGTLSGNPLAVAAGLTTLRLI
QAPGFYDKLTAQTRKLADGLSEAARAAGVPFAADAIGGMFGIYFRDGVPGSFAEVTKSDTARFNRFFHAMLDNGVYLAPS
AFEAGFVSACHDDAILQATLDAARKAFAA
>Mature_428_residues
SRNQQLFDRAQQTIPGGVNSPVRAFRSVGGTPRFITCAEGAYMWDADGQRYIDYIGSWGPMIVGHAHPDVVRAVQETAAH
SFSFGAPTEAEIDMAEEICKLVPSIEQVRLVSSGTEATMSALRLARGFTGRDLIIKFEGCYHGHADSLLVKAGSGLLTFA
DTTQNAPSSAGVPADVTRHTMVLEYNNVAQLEEAFAQHRGEIAAVIVEVVAGNMNLVRASDAFLKAMRELCTRDGAVLIF
DEVMTGFRVALGGAQSHYGITPDMTCLGKVIGGGMPAAAFGGRRDIMAKLAPLGGVYQAGTLSGNPLAVAAGLTTLRLIQ
APGFYDKLTAQTRKLADGLSEAARAAGVPFAADAIGGMFGIYFRDGVPGSFAEVTKSDTARFNRFFHAMLDNGVYLAPSA
FEAGFVSACHDDAILQATLDAARKAFAA

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=365, Percent_Identity=27.1232876712329, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI24119277, Length=317, Percent_Identity=26.813880126183, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI37574042, Length=349, Percent_Identity=26.0744985673352, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI226442705, Length=348, Percent_Identity=25, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI226442709, Length=318, Percent_Identity=25.1572327044025, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI284507298, Length=216, Percent_Identity=29.1666666666667, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1786349, Length=429, Percent_Identity=59.2074592074592, Blast_Score=521, Evalue=1e-149,
Organism=Escherichia coli, GI1789016, Length=385, Percent_Identity=31.1688311688312, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI1788044, Length=350, Percent_Identity=30.2857142857143, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1787560, Length=366, Percent_Identity=30.6010928961749, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1789759, Length=313, Percent_Identity=28.7539936102236, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI145693181, Length=315, Percent_Identity=30.4761904761905, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI1786991, Length=387, Percent_Identity=28.1653746770026, Blast_Score=101, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI25144271, Length=401, Percent_Identity=24.6882793017456, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71992977, Length=380, Percent_Identity=24.4736842105263, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI32564660, Length=323, Percent_Identity=27.8637770897833, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI25144274, Length=274, Percent_Identity=24.0875912408759, Blast_Score=75, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6323470, Length=309, Percent_Identity=26.8608414239482, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6324432, Length=327, Percent_Identity=24.4648318042813, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21357415, Length=322, Percent_Identity=29.1925465838509, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI21356575, Length=323, Percent_Identity=28.1733746130031, Blast_Score=94, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =5.4.3.8 [H]

Molecular weight: Translated: 45370; Mature: 45239

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRNQQLFDRAQQTIPGGVNSPVRAFRSVGGTPRFITCAEGAYMWDADGQRYIDYIGSWG
CCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCEEEECCCCEEECCCCHHHHHHHCCCC
PMIVGHAHPDVVRAVQETAAHSFSFGAPTEAEIDMAEEICKLVPSIEQVRLVSSGTEATM
CEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCHHHHH
SALRLARGFTGRDLIIKFEGCYHGHADSLLVKAGSGLLTFADTTQNAPSSAGVPADVTRH
HHHHHHHCCCCCEEEEEEECCCCCCCCCEEEECCCCEEEEECCCCCCCCCCCCCCCCCCC
TMVLEYNNVAQLEEAFAQHRGEIAAVIVEVVAGNMNLVRASDAFLKAMRELCTRDGAVLI
EEEEEECCHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHCCCCCEEE
FDEVMTGFRVALGGAQSHYGITPDMTCLGKVIGGGMPAAAFGGRRDIMAKLAPLGGVYQA
HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCHHHCCCHHHHHHHHHCCCCCEEC
GTLSGNPLAVAAGLTTLRLIQAPGFYDKLTAQTRKLADGLSEAARAAGVPFAADAIGGMF
CCCCCCCEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
GIYFRDGVPGSFAEVTKSDTARFNRFFHAMLDNGVYLAPSAFEAGFVSACHDDAILQATL
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHCCHHHHHHHH
DAARKAFAA
HHHHHHHCC
>Mature Secondary Structure 
SRNQQLFDRAQQTIPGGVNSPVRAFRSVGGTPRFITCAEGAYMWDADGQRYIDYIGSWG
CCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCEEEECCCCEEECCCCHHHHHHHCCCC
PMIVGHAHPDVVRAVQETAAHSFSFGAPTEAEIDMAEEICKLVPSIEQVRLVSSGTEATM
CEEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCHHHHH
SALRLARGFTGRDLIIKFEGCYHGHADSLLVKAGSGLLTFADTTQNAPSSAGVPADVTRH
HHHHHHHCCCCCEEEEEEECCCCCCCCCEEEECCCCEEEEECCCCCCCCCCCCCCCCCCC
TMVLEYNNVAQLEEAFAQHRGEIAAVIVEVVAGNMNLVRASDAFLKAMRELCTRDGAVLI
EEEEEECCHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHCCCCCEEE
FDEVMTGFRVALGGAQSHYGITPDMTCLGKVIGGGMPAAAFGGRRDIMAKLAPLGGVYQA
HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCHHHCCCHHHHHHHHHCCCCCEEC
GTLSGNPLAVAAGLTTLRLIQAPGFYDKLTAQTRKLADGLSEAARAAGVPFAADAIGGMF
CCCCCCCEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
GIYFRDGVPGSFAEVTKSDTARFNRFFHAMLDNGVYLAPSAFEAGFVSACHDDAILQATL
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHCCHHHHHHHH
DAARKAFAA
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA