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The map label for this gene is rfaI [H]

Identifier: 73542243

GI number: 73542243

Start: 2802854

End: 2803849

Strand: Direct

Name: rfaI [H]

Synonym: Reut_A2558

Alternate gene names: 73542243

Gene position: 2802854-2803849 (Clockwise)

Preceding gene: 73542242

Following gene: 73542244

Centisome position: 73.63

GC content: 57.73

Gene sequence:

>996_bases
ATGAACCTGCCCGCATCCGACAACAACACCATGGCTCCGATGACAGGCAGCAACGGCAAGCCGTCGTTCCATATCGCGTT
CTGCGTCGACGACAATTATTTCCGTGCGATGGGCGCGACGATCGCATCGATCATCGACAATAACCCGGGACAGCATTTCA
CCTTCCATGTGCTGACGTTCTCCGCGCTGGAAGAAAATCAGCGCCGCCTGAAGCAGCTCGAAGAGATGTACCCGGTCAGC
ACCCAGCTGCACTTGCTGGATCTGGCATCCTTCACGCAGTTCTCGCATTTCCTCGGGCACTCGCACTACTCGCTGTCGAT
CTTTACTCGACTGGTCATTCCCGAGGTGCTGCAAGGGCAGACCGACCGCGTGCTGTACCTCGATGCCGATATCCTGTGCG
TCAACCGCCTCGACGAACTGGTCGACATGGATATCAGCAACGAGATCGCGGTCGTCGTGCCGGATGCGCCCGTTACACTG
CGCCGGCGTGTTGCCGCACTTGGCCTGGCCCATGCCGAGTATTTCAATGGCGGCGTGCTGTTCATCAATATCGACAAGTG
GCTGGCCGAAAACATCACGCCTCAAACCCTGGAAGCGCTACTTGATACCAGCACGGACATGCGTTTCAACGACCAGGACG
CGCTCAATAAGGTGCTCAACGGTCGCGCGAAATACATCTCGCCGCGCTGGAATTACCTATATGACCTGATCCACGACCTG
AACGTCAACCGCTTTGCCATGCGTCCGGTCGGCAAGGCGGTCTTTATCCATTTTGCGGGCTCGGTCAAACCCTGGGCCGA
CTGGAGCGGCCATGAGGCCCGCGGACTGTTCCGCAAATATCTGGCCTTGTCACCGTGGCGTGACATGCCGCTGGATCCGG
AACCGAGAAATACCAAGGAAATGCGCATGCATTCGCGCTTCATGTTCCGCCAGCACAAGCCGGTGGAGAGCCTGAAATGG
TATTTGCGGTATTTGCGCAAGCGGGCGCAACGGTAA

Upstream 100 bases:

>100_bases
GGGTGCCGATCGACAGGCAGAACAGCACGGTGATCACCGCAGCGAATCGCCGGGACTGGGTCAATGCCATTCCCGTCGAA
CAAGTCATGAAGACCATCCA

Downstream 100 bases:

>100_bases
TGCCTGAGTGGCATGCCCCCACCCTGCTCTGACATTGAAGATAAATGGCAATCCGAAGCAACCACATACTGGTCAGCCGC
ACCGACAACATCGGCGACAT

Product: glycosyl transferase family protein

Products: NA

Alternate protein names: Lipopolysaccharide 3-alpha-galactosyltransferase [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MNLPASDNNTMAPMTGSNGKPSFHIAFCVDDNYFRAMGATIASIIDNNPGQHFTFHVLTFSALEENQRRLKQLEEMYPVS
TQLHLLDLASFTQFSHFLGHSHYSLSIFTRLVIPEVLQGQTDRVLYLDADILCVNRLDELVDMDISNEIAVVVPDAPVTL
RRRVAALGLAHAEYFNGGVLFINIDKWLAENITPQTLEALLDTSTDMRFNDQDALNKVLNGRAKYISPRWNYLYDLIHDL
NVNRFAMRPVGKAVFIHFAGSVKPWADWSGHEARGLFRKYLALSPWRDMPLDPEPRNTKEMRMHSRFMFRQHKPVESLKW
YLRYLRKRAQR

Sequences:

>Translated_331_residues
MNLPASDNNTMAPMTGSNGKPSFHIAFCVDDNYFRAMGATIASIIDNNPGQHFTFHVLTFSALEENQRRLKQLEEMYPVS
TQLHLLDLASFTQFSHFLGHSHYSLSIFTRLVIPEVLQGQTDRVLYLDADILCVNRLDELVDMDISNEIAVVVPDAPVTL
RRRVAALGLAHAEYFNGGVLFINIDKWLAENITPQTLEALLDTSTDMRFNDQDALNKVLNGRAKYISPRWNYLYDLIHDL
NVNRFAMRPVGKAVFIHFAGSVKPWADWSGHEARGLFRKYLALSPWRDMPLDPEPRNTKEMRMHSRFMFRQHKPVESLKW
YLRYLRKRAQR
>Mature_331_residues
MNLPASDNNTMAPMTGSNGKPSFHIAFCVDDNYFRAMGATIASIIDNNPGQHFTFHVLTFSALEENQRRLKQLEEMYPVS
TQLHLLDLASFTQFSHFLGHSHYSLSIFTRLVIPEVLQGQTDRVLYLDADILCVNRLDELVDMDISNEIAVVVPDAPVTL
RRRVAALGLAHAEYFNGGVLFINIDKWLAENITPQTLEALLDTSTDMRFNDQDALNKVLNGRAKYISPRWNYLYDLIHDL
NVNRFAMRPVGKAVFIHFAGSVKPWADWSGHEARGLFRKYLALSPWRDMPLDPEPRNTKEMRMHSRFMFRQHKPVESLKW
YLRYLRKRAQR

Specific function: Adds the galactose(I) group on the glucose(I) group of LPS [H]

COG id: COG1442

COG function: function code M; Lipopolysaccharide biosynthesis proteins, LPS:glycosyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 8 family [H]

Homologues:

Organism=Escherichia coli, GI1790057, Length=313, Percent_Identity=25.8785942492013, Blast_Score=128, Evalue=7e-31,
Organism=Escherichia coli, GI1790056, Length=333, Percent_Identity=24.9249249249249, Blast_Score=111, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002495
- InterPro:   IPR013645 [H]

Pfam domain/function: PF01501 Glyco_transf_8; PF08437 Glyco_transf_8N [H]

EC number: =2.4.1.44 [H]

Molecular weight: Translated: 38213; Mature: 38213

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLPASDNNTMAPMTGSNGKPSFHIAFCVDDNYFRAMGATIASIIDNNPGQHFTFHVLTF
CCCCCCCCCEECCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCEEEEEEEEH
SALEENQRRLKQLEEMYPVSTQLHLLDLASFTQFSHFLGHSHYSLSIFTRLVIPEVLQGQ
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
TDRVLYLDADILCVNRLDELVDMDISNEIAVVVPDAPVTLRRRVAALGLAHAEYFNGGVL
CCEEEEEECCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCEE
FINIDKWLAENITPQTLEALLDTSTDMRFNDQDALNKVLNGRAKYISPRWNYLYDLIHDL
EEECHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCEEECCHHHHHHHHHHHC
NVNRFAMRPVGKAVFIHFAGSVKPWADWSGHEARGLFRKYLALSPWRDMPLDPEPRNTKE
CCCEEEECCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHH
MRMHSRFMFRQHKPVESLKWYLRYLRKRAQR
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNLPASDNNTMAPMTGSNGKPSFHIAFCVDDNYFRAMGATIASIIDNNPGQHFTFHVLTF
CCCCCCCCCEECCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCCEEEEEEEEH
SALEENQRRLKQLEEMYPVSTQLHLLDLASFTQFSHFLGHSHYSLSIFTRLVIPEVLQGQ
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
TDRVLYLDADILCVNRLDELVDMDISNEIAVVVPDAPVTLRRRVAALGLAHAEYFNGGVL
CCEEEEEECCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCEE
FINIDKWLAENITPQTLEALLDTSTDMRFNDQDALNKVLNGRAKYISPRWNYLYDLIHDL
EEECHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCEEECCHHHHHHHHHHHC
NVNRFAMRPVGKAVFIHFAGSVKPWADWSGHEARGLFRKYLALSPWRDMPLDPEPRNTKE
CCCEEEECCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHH
MRMHSRFMFRQHKPVESLKWYLRYLRKRAQR
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2235496; 9535865; 11677609 [H]