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The map label for this gene is purD [H]

Identifier: 73542208

GI number: 73542208

Start: 2765767

End: 2767035

Strand: Direct

Name: purD [H]

Synonym: Reut_A2523

Alternate gene names: 73542208

Gene position: 2765767-2767035 (Clockwise)

Preceding gene: 73542207

Following gene: 73542209

Centisome position: 72.66

GC content: 66.67

Gene sequence:

>1269_bases
ATGAAAGTATTGGTTGTCGGCTCGGGTGGACGTGAACACGCGCTGGCCTGGAAATTGGCCCAATCCCCCAAGCTACAGGT
GGTGTACGTGGCACCGGGCAACGGCGGGACCGCGCTCGACAAGCGCCTGCAGAATGTTCCGCTGACGGATCCGGAAGTGC
TGGCCGCGTTTGCCGAGCGCGAAGGCGTGGCCTTTACCGTGGTCGGCCCCGAAGCGCCGCTGGCTGGGGGCATCGTGGAC
GTGTTCCGCGGCAAGGGCCTGCGCATCTTTGGCCCGACCAAAGCTGCCGCGCAGCTCGAGTCGTCGAAGGATTTCGCCAA
GGCCTTCATGCATCGTCACGGCATTCCGACGGCGGCCTACCAGACCTTCTCCGATGCCGCCCAGGCGCACGCCTATATCG
ACGCGCAGGGCGCGCCGATCGTGATCAAGGCTGACGGCCTGGCCGCCGGCAAGGGTGTGGTCGTCGCCATGAGCCTGGAA
GAAGCGCACTCGGCGGTCGACATGATGCTGGCCGACAACAAGCTCGGCGACGCTGGCGCGCGCGTGGTGGTCGAGGAATT
CCTCGAAGGCGAGGAAGCGAGCTTCATCGTGCTGGTCGACGGCAAGAACGTGCTGGCGCTGGCCACCAGCCAGGACCACA
AGCGCCTGCTGGACGCCGACGCCGGCCCCAACACCGGCGGCATGGGCGCCTACTCGCCCGCGCCGGTGGTCACGCCGGCC
CTGCATGCGCGCGCGCTGCGCGAGATCATCATGCCGACCGTGCGCGGCATGGAGAAGGACGGCATTCCGTACACGGGCTT
CCTGTACGCAGGCCTGATGATCGACAAGGACGGCAACCTGAAGACGCTGGAATTCAACTGCCGCATGGGCGATCCGGAAA
CCCAGCCGATCATGGCTCGCCTGAAGACCGACCTGGTCGACGTGATGGACGCCGCCGTGTCGGGCAAGCTTGACGAAGTC
GAGCTGGACTGGGACCGCCGCACCGCGCTGGGCGTCGTGATGGCCGCTTACGGCTATCCGGATGCGCCGCGCAAGGGCGA
TGCTATTACCGGCATCCCAGCCGAAACCGACGACAGCGTGACCTTCCACGCCGGCACCACGCTCAAGGACGGCACGCTGC
TGACCACGGGCGGACGCGTGCTGTGCGTGGTCGGCCTGGCCGATACCGTCAAGGCTGCGCAACGCGCCGCCTACGCCGCC
GTGGAGCAGATCCGCTTCGATGGCATGCAATACCGTACCGACATCGGCTACCGCGCCATCAAGCGCTGA

Upstream 100 bases:

>100_bases
CAACGCGGTCATTGAAGAATAACTAGACTGAATTAGCCCCGGGCGGCAGCACACTCACGCTGCCGCCCTTTTGCATGCGT
TTCTTTTCTGGCAGTGGATC

Downstream 100 bases:

>100_bases
CCGCCGATATGGCGCCGCCATGGGGCCGGGCGGCTCCGGGCGGCCGGTCACGTTCCGACGGCACGCGCGGGCCAGAGGCT
CGCCAGCCGTTACAATCGTG

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MKVLVVGSGGREHALAWKLAQSPKLQVVYVAPGNGGTALDKRLQNVPLTDPEVLAAFAEREGVAFTVVGPEAPLAGGIVD
VFRGKGLRIFGPTKAAAQLESSKDFAKAFMHRHGIPTAAYQTFSDAAQAHAYIDAQGAPIVIKADGLAAGKGVVVAMSLE
EAHSAVDMMLADNKLGDAGARVVVEEFLEGEEASFIVLVDGKNVLALATSQDHKRLLDADAGPNTGGMGAYSPAPVVTPA
LHARALREIIMPTVRGMEKDGIPYTGFLYAGLMIDKDGNLKTLEFNCRMGDPETQPIMARLKTDLVDVMDAAVSGKLDEV
ELDWDRRTALGVVMAAYGYPDAPRKGDAITGIPAETDDSVTFHAGTTLKDGTLLTTGGRVLCVVGLADTVKAAQRAAYAA
VEQIRFDGMQYRTDIGYRAIKR

Sequences:

>Translated_422_residues
MKVLVVGSGGREHALAWKLAQSPKLQVVYVAPGNGGTALDKRLQNVPLTDPEVLAAFAEREGVAFTVVGPEAPLAGGIVD
VFRGKGLRIFGPTKAAAQLESSKDFAKAFMHRHGIPTAAYQTFSDAAQAHAYIDAQGAPIVIKADGLAAGKGVVVAMSLE
EAHSAVDMMLADNKLGDAGARVVVEEFLEGEEASFIVLVDGKNVLALATSQDHKRLLDADAGPNTGGMGAYSPAPVVTPA
LHARALREIIMPTVRGMEKDGIPYTGFLYAGLMIDKDGNLKTLEFNCRMGDPETQPIMARLKTDLVDVMDAAVSGKLDEV
ELDWDRRTALGVVMAAYGYPDAPRKGDAITGIPAETDDSVTFHAGTTLKDGTLLTTGGRVLCVVGLADTVKAAQRAAYAA
VEQIRFDGMQYRTDIGYRAIKR
>Mature_422_residues
MKVLVVGSGGREHALAWKLAQSPKLQVVYVAPGNGGTALDKRLQNVPLTDPEVLAAFAEREGVAFTVVGPEAPLAGGIVD
VFRGKGLRIFGPTKAAAQLESSKDFAKAFMHRHGIPTAAYQTFSDAAQAHAYIDAQGAPIVIKADGLAAGKGVVVAMSLE
EAHSAVDMMLADNKLGDAGARVVVEEFLEGEEASFIVLVDGKNVLALATSQDHKRLLDADAGPNTGGMGAYSPAPVVTPA
LHARALREIIMPTVRGMEKDGIPYTGFLYAGLMIDKDGNLKTLEFNCRMGDPETQPIMARLKTDLVDVMDAAVSGKLDEV
ELDWDRRTALGVVMAAYGYPDAPRKGDAITGIPAETDDSVTFHAGTTLKDGTLLTTGGRVLCVVGLADTVKAAQRAAYAA
VEQIRFDGMQYRTDIGYRAIKR

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=424, Percent_Identity=50, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI4503915, Length=424, Percent_Identity=50, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI209869995, Length=424, Percent_Identity=50, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI209869993, Length=424, Percent_Identity=50, Blast_Score=402, Evalue=1e-112,
Organism=Escherichia coli, GI1790438, Length=426, Percent_Identity=62.4413145539906, Blast_Score=520, Evalue=1e-148,
Organism=Caenorhabditis elegans, GI17567511, Length=421, Percent_Identity=43.2304038004751, Blast_Score=330, Evalue=1e-90,
Organism=Saccharomyces cerevisiae, GI6321203, Length=445, Percent_Identity=44.2696629213483, Blast_Score=346, Evalue=5e-96,
Organism=Drosophila melanogaster, GI62471619, Length=432, Percent_Identity=48.6111111111111, Blast_Score=359, Evalue=2e-99,
Organism=Drosophila melanogaster, GI24582400, Length=432, Percent_Identity=48.6111111111111, Blast_Score=358, Evalue=3e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 44635; Mature: 44635

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLVVGSGGREHALAWKLAQSPKLQVVYVAPGNGGTALDKRLQNVPLTDPEVLAAFAER
CEEEEEECCCCCCEEEEEECCCCCEEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHC
EGVAFTVVGPEAPLAGGIVDVFRGKGLRIFGPTKAAAQLESSKDFAKAFMHRHGIPTAAY
CCEEEEEECCCCCCCCCHHHHHCCCCEEEECCCHHHHHHHCCHHHHHHHHHHCCCCHHHH
QTFSDAAQAHAYIDAQGAPIVIKADGLAAGKGVVVAMSLEEAHSAVDMMLADNKLGDAGA
HHHHHHHHHEEEEECCCCEEEEEECCCCCCCCEEEEEEHHHHHHHHHHHEECCCCCCCHH
RVVVEEFLEGEEASFIVLVDGKNVLALATSQDHKRLLDADAGPNTGGMGAYSPAPVVTPA
HHHHHHHHCCCCCEEEEEECCCCEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCCCCHH
LHARALREIIMPTVRGMEKDGIPYTGFLYAGLMIDKDGNLKTLEFNCRMGDPETQPIMAR
HHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEEECCCCEEEEEEEEECCCCCCHHHHHH
LKTDLVDVMDAAVSGKLDEVELDWDRRTALGVVMAAYGYPDAPRKGDAITGIPAETDDSV
HHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCE
TFHAGTTLKDGTLLTTGGRVLCVVGLADTVKAAQRAAYAAVEQIRFDGMQYRTDIGYRAI
EEEECCEECCCEEEECCCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHH
KR
CC
>Mature Secondary Structure
MKVLVVGSGGREHALAWKLAQSPKLQVVYVAPGNGGTALDKRLQNVPLTDPEVLAAFAER
CEEEEEECCCCCCEEEEEECCCCCEEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHC
EGVAFTVVGPEAPLAGGIVDVFRGKGLRIFGPTKAAAQLESSKDFAKAFMHRHGIPTAAY
CCEEEEEECCCCCCCCCHHHHHCCCCEEEECCCHHHHHHHCCHHHHHHHHHHCCCCHHHH
QTFSDAAQAHAYIDAQGAPIVIKADGLAAGKGVVVAMSLEEAHSAVDMMLADNKLGDAGA
HHHHHHHHHEEEEECCCCEEEEEECCCCCCCCEEEEEEHHHHHHHHHHHEECCCCCCCHH
RVVVEEFLEGEEASFIVLVDGKNVLALATSQDHKRLLDADAGPNTGGMGAYSPAPVVTPA
HHHHHHHHCCCCCEEEEEECCCCEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCCCCHH
LHARALREIIMPTVRGMEKDGIPYTGFLYAGLMIDKDGNLKTLEFNCRMGDPETQPIMAR
HHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEEECCCCEEEEEEEEECCCCCCHHHHHH
LKTDLVDVMDAAVSGKLDEVELDWDRRTALGVVMAAYGYPDAPRKGDAITGIPAETDDSV
HHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCE
TFHAGTTLKDGTLLTTGGRVLCVVGLADTVKAAQRAAYAAVEQIRFDGMQYRTDIGYRAI
EEEECCEECCCEEEECCCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHH
KR
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]