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The map label for this gene is rluB [H]

Identifier: 73541719

GI number: 73541719

Start: 2229895

End: 2231742

Strand: Reverse

Name: rluB [H]

Synonym: Reut_A2031

Alternate gene names: 73541719

Gene position: 2231742-2229895 (Counterclockwise)

Preceding gene: 73541720

Following gene: 73541718

Centisome position: 58.63

GC content: 68.13

Gene sequence:

>1848_bases
ATGCAACATCCGATAGTGAATACTTTGTCTGACTCCGCTGAACAAGACACCCGGGCCGCCGGGGCACCGGCCGCCGATGG
CGCTGCAGAATCCGGATCAGGCTCCGATGGCGCGCCGCGCAGGAAAGGGCTGAGGCGCGGCTTGCGCAACCTGGTGGCTT
CGCGCCGGCAGGCTGCGCAGGATCGCGACGACCGCGGCGAGGAGACGGAATCTTCGGCACCGGCCGATGCGGGCGAGGGC
AAGGCTGCCGGCGAGGCCGGCGGACGTGCCGCCCCGCGTGGCCGCGGCAAGCGCAAGCCGGCCAGGGCCGAAGGCACCGC
GCCGCGTGAAGGCGGCCAACCCGCGCAGGAAGGTGGCGAAAAGGCCGAGTCGGCTGGCCGCAAGCGCAAGCCACAAGGCC
AGCGTGCAGCCAGGGTTTCTCCGGAAGGTGACGCCCAGCCGGCAGCCCGGCAACAGGGCCGACGCAAGGGCGGCCCCCAG
CAGGGTGGTGTCCAGCAACAGCGCAAGGGTCAACGCGGCGCCGAGAAGTCGGCCGCAGGCAGCGAAGACCTGTTCCGTTT
CGTCATTTCCGACCAGTACGACCAGGAAGACACCGTCATTCCCCGCACCAAGGCAAAGCCGGTGCGCGAACTGTCGGCCG
ACGACGATGCACCGAAGCTGCACAAGGTCCTGGCCGAAGGCGGCCTTGGCTCGCGGCGCGAAATGGAAGAGCTGATCCTG
CAGGGCCGCGTCTCCGTCAACGGACTGCCCGCGCATATCGGCCAGCGGATCCTGCCGACCGATCAGGTGCGCGTAAACGG
CAAGCTGATCCACCGCAAAGTCTCCACCAAGCCGCCGCGCGTGCTGCTGTACCACAAGCCGGCCGGTGAAATCGTCAGCC
AGTCAGATCCGGAAGGCCGTCCGACCGTGTTCGATAGCCTGCCGCGCATGAAGACCGGCAAGTGGGTCGCCGTGGGGCGC
CTGGACTTCAATACCGAAGGCCTGCTGATCTTTACGACGTCCGGCGATATCGCCAACCGCTTCATGCATCCGCGCTATGG
CGTGGAGCGCGAGTACGCGGTGCGTACGCTGGGCGAACTGGCGGAAGCCGATCGCCAGCGTCTGCTGCATGGCATCAAGC
TCGATGATGGCGAAGCCAATTTCCTGCGCATTGCGGATGGCGGCGGCGAGGGCGTGAACCAGTGGTATCACGTGGCGCTG
ACCGAAGGCCGCAACCGCGAAGTGCGGCGCATGTTTGAGGCGGTTGGCCTGACTGTGTCGCGTCTGATCCGCACGCGCTA
CGGCCAGTTCCTGCTGCCGCGCGGCCTCAAGCGTGGCCGCTGGCAGGAGCTGGAGGCGAACGAGGTCAAGTCGATGATGA
CTGCGATCGGTCTGAAGGTGCCCGGCACGAGCGAGCAGGGCGGCAAGGGCGTGACCAAGGTCGGCGGGCGCAAGCAGCGT
ACCGAGGCGGCGATCGCAGGCATGCCCATGCACACCGGCATGGACGGTTTGCCGCGCGTCGACAAGGGCGGCGCTGGTCG
TGGTCAGCCGGATCCGATGCGCACGTCGATGGGCTATATCAGCCATGGTCCGACCGTGTTGACGTCGCACGCCGCCAATC
TCGGTGGTGGGCGCGGGCAGGGTGGCGGGCGTGCTGGTGGTGCCGGCCGTGGCACGGGCATGGGACTGGGTGGCCAGGGT
CGGCCCCAGCGCCGCGGCGGTGGCGATGTCAACGGCAATGTGATGCCGAAGGCGGCCAAGTCCGGTGGCAGCGGTGGCGG
TGGTGGTGGTGGTGGCAACCGCCCGGGCCGTGGCAACAAGGGTGGCGGAGGCAACAATCGCGGTGGCAATCGCGGCGGAA
ACCGCTGA

Upstream 100 bases:

>100_bases
ACGCGCCGGGCGATCAGGACGGCGGCAATCCATCCGGCGACGACGACGCTGCGCCGCCGCACGCGAACGAGCACGATGAG
CGCGTGCCCAACTGAACAAT

Downstream 100 bases:

>100_bases
ATCTTCCCGCTCCAACCCCGGCGGCTAGCGCCTGCCGGGGATCCATTACGCGCCGATACATGCCCTTTTGCCTTTTGGAC
ATAATCTGCGTATAATTAAA

Product: ribosomal large subunit pseudouridine synthase B

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 615; Mature: 615

Protein sequence:

>615_residues
MQHPIVNTLSDSAEQDTRAAGAPAADGAAESGSGSDGAPRRKGLRRGLRNLVASRRQAAQDRDDRGEETESSAPADAGEG
KAAGEAGGRAAPRGRGKRKPARAEGTAPREGGQPAQEGGEKAESAGRKRKPQGQRAARVSPEGDAQPAARQQGRRKGGPQ
QGGVQQQRKGQRGAEKSAAGSEDLFRFVISDQYDQEDTVIPRTKAKPVRELSADDDAPKLHKVLAEGGLGSRREMEELIL
QGRVSVNGLPAHIGQRILPTDQVRVNGKLIHRKVSTKPPRVLLYHKPAGEIVSQSDPEGRPTVFDSLPRMKTGKWVAVGR
LDFNTEGLLIFTTSGDIANRFMHPRYGVEREYAVRTLGELAEADRQRLLHGIKLDDGEANFLRIADGGGEGVNQWYHVAL
TEGRNREVRRMFEAVGLTVSRLIRTRYGQFLLPRGLKRGRWQELEANEVKSMMTAIGLKVPGTSEQGGKGVTKVGGRKQR
TEAAIAGMPMHTGMDGLPRVDKGGAGRGQPDPMRTSMGYISHGPTVLTSHAANLGGGRGQGGGRAGGAGRGTGMGLGGQG
RPQRRGGGDVNGNVMPKAAKSGGSGGGGGGGGNRPGRGNKGGGGNNRGGNRGGNR

Sequences:

>Translated_615_residues
MQHPIVNTLSDSAEQDTRAAGAPAADGAAESGSGSDGAPRRKGLRRGLRNLVASRRQAAQDRDDRGEETESSAPADAGEG
KAAGEAGGRAAPRGRGKRKPARAEGTAPREGGQPAQEGGEKAESAGRKRKPQGQRAARVSPEGDAQPAARQQGRRKGGPQ
QGGVQQQRKGQRGAEKSAAGSEDLFRFVISDQYDQEDTVIPRTKAKPVRELSADDDAPKLHKVLAEGGLGSRREMEELIL
QGRVSVNGLPAHIGQRILPTDQVRVNGKLIHRKVSTKPPRVLLYHKPAGEIVSQSDPEGRPTVFDSLPRMKTGKWVAVGR
LDFNTEGLLIFTTSGDIANRFMHPRYGVEREYAVRTLGELAEADRQRLLHGIKLDDGEANFLRIADGGGEGVNQWYHVAL
TEGRNREVRRMFEAVGLTVSRLIRTRYGQFLLPRGLKRGRWQELEANEVKSMMTAIGLKVPGTSEQGGKGVTKVGGRKQR
TEAAIAGMPMHTGMDGLPRVDKGGAGRGQPDPMRTSMGYISHGPTVLTSHAANLGGGRGQGGGRAGGAGRGTGMGLGGQG
RPQRRGGGDVNGNVMPKAAKSGGSGGGGGGGGNRPGRGNKGGGGNNRGGNRGGNR
>Mature_615_residues
MQHPIVNTLSDSAEQDTRAAGAPAADGAAESGSGSDGAPRRKGLRRGLRNLVASRRQAAQDRDDRGEETESSAPADAGEG
KAAGEAGGRAAPRGRGKRKPARAEGTAPREGGQPAQEGGEKAESAGRKRKPQGQRAARVSPEGDAQPAARQQGRRKGGPQ
QGGVQQQRKGQRGAEKSAAGSEDLFRFVISDQYDQEDTVIPRTKAKPVRELSADDDAPKLHKVLAEGGLGSRREMEELIL
QGRVSVNGLPAHIGQRILPTDQVRVNGKLIHRKVSTKPPRVLLYHKPAGEIVSQSDPEGRPTVFDSLPRMKTGKWVAVGR
LDFNTEGLLIFTTSGDIANRFMHPRYGVEREYAVRTLGELAEADRQRLLHGIKLDDGEANFLRIADGGGEGVNQWYHVAL
TEGRNREVRRMFEAVGLTVSRLIRTRYGQFLLPRGLKRGRWQELEANEVKSMMTAIGLKVPGTSEQGGKGVTKVGGRKQR
TEAAIAGMPMHTGMDGLPRVDKGGAGRGQPDPMRTSMGYISHGPTVLTSHAANLGGGRGQGGGRAGGAGRGTGMGLGGQG
RPQRRGGGDVNGNVMPKAAKSGGSGGGGGGGGNRPGRGNKGGGGNNRGGNRGGNR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=253, Percent_Identity=53.3596837944664, Blast_Score=262, Evalue=4e-71,
Organism=Escherichia coli, GI1790453, Length=251, Percent_Identity=30.2788844621514, Blast_Score=100, Evalue=5e-22,
Organism=Escherichia coli, GI1788510, Length=213, Percent_Identity=30.0469483568075, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI87081838, Length=193, Percent_Identity=31.0880829015544, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 64713; Mature: 64713

Theoretical pI: Translated: 11.29; Mature: 11.29

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHPIVNTLSDSAEQDTRAAGAPAADGAAESGSGSDGAPRRKGLRRGLRNLVASRRQAAQ
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
DRDDRGEETESSAPADAGEGKAAGEAGGRAAPRGRGKRKPARAEGTAPREGGQPAQEGGE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
KAESAGRKRKPQGQRAARVSPEGDAQPAARQQGRRKGGPQQGGVQQQRKGQRGAEKSAAG
HHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHCCCCCHHHCCC
SEDLFRFVISDQYDQEDTVIPRTKAKPVRELSADDDAPKLHKVLAEGGLGSRREMEELIL
HHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHH
QGRVSVNGLPAHIGQRILPTDQVRVNGKLIHRKVSTKPPRVLLYHKPAGEIVSQSDPEGR
HCCCCCCCCHHHHCCCCCCCCCEEECCEEEEEECCCCCCEEEEEECCCHHHHCCCCCCCC
PTVFDSLPRMKTGKWVAVGRLDFNTEGLLIFTTSGDIANRFMHPRYGVEREYAVRTLGEL
CHHHHCCCCCCCCCEEEEEEEEECCCCEEEEECCCHHHHHHCCCCCCCCHHHHHHHHHHH
AEADRQRLLHGIKLDDGEANFLRIADGGGEGVNQWYHVALTEGRNREVRRMFEAVGLTVS
HHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHH
RLIRTRYGQFLLPRGLKRGRWQELEANEVKSMMTAIGLKVPGTSEQGGKGVTKVGGRKQR
HHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCHHH
TEAAIAGMPMHTGMDGLPRVDKGGAGRGQPDPMRTSMGYISHGPTVLTSHAANLGGGRGQ
HHHHHHCCCHHCCCCCCCCCCCCCCCCCCCCCHHHHCCHHCCCCEEEHHHHHHCCCCCCC
GGGRAGGAGRGTGMGLGGQGRPQRRGGGDVNGNVMPKAAKSGGSGGGGGGGGNRPGRGNK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCC
GGGGNNRGGNRGGNR
CCCCCCCCCCCCCCC
>Mature Secondary Structure
MQHPIVNTLSDSAEQDTRAAGAPAADGAAESGSGSDGAPRRKGLRRGLRNLVASRRQAAQ
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
DRDDRGEETESSAPADAGEGKAAGEAGGRAAPRGRGKRKPARAEGTAPREGGQPAQEGGE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
KAESAGRKRKPQGQRAARVSPEGDAQPAARQQGRRKGGPQQGGVQQQRKGQRGAEKSAAG
HHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHCCCCCHHHCCC
SEDLFRFVISDQYDQEDTVIPRTKAKPVRELSADDDAPKLHKVLAEGGLGSRREMEELIL
HHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHH
QGRVSVNGLPAHIGQRILPTDQVRVNGKLIHRKVSTKPPRVLLYHKPAGEIVSQSDPEGR
HCCCCCCCCHHHHCCCCCCCCCEEECCEEEEEECCCCCCEEEEEECCCHHHHCCCCCCCC
PTVFDSLPRMKTGKWVAVGRLDFNTEGLLIFTTSGDIANRFMHPRYGVEREYAVRTLGEL
CHHHHCCCCCCCCCEEEEEEEEECCCCEEEEECCCHHHHHHCCCCCCCCHHHHHHHHHHH
AEADRQRLLHGIKLDDGEANFLRIADGGGEGVNQWYHVALTEGRNREVRRMFEAVGLTVS
HHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHH
RLIRTRYGQFLLPRGLKRGRWQELEANEVKSMMTAIGLKVPGTSEQGGKGVTKVGGRKQR
HHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCHHH
TEAAIAGMPMHTGMDGLPRVDKGGAGRGQPDPMRTSMGYISHGPTVLTSHAANLGGGRGQ
HHHHHHCCCHHCCCCCCCCCCCCCCCCCCCCCHHHHCCHHCCCCEEEHHHHHHCCCCCCC
GGGRAGGAGRGTGMGLGGQGRPQRRGGGDVNGNVMPKAAKSGGSGGGGGGGGNRPGRGNK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCC
GGGGNNRGGNRGGNR
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]