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The map label for this gene is dusA [H]

Identifier: 73541705

GI number: 73541705

Start: 2209954

End: 2210979

Strand: Reverse

Name: dusA [H]

Synonym: Reut_A2017

Alternate gene names: 73541705

Gene position: 2210979-2209954 (Counterclockwise)

Preceding gene: 73541706

Following gene: 73541703

Centisome position: 58.08

GC content: 63.16

Gene sequence:

>1026_bases
ATGAAAGTAAGCCCCCGCCGCATCTCGGTTGCCCCGATGATGGACTGGACCGATCGCCATTGCCGCACCTTCCATCGCCA
GCTCAGCCGCCATACCTGGCTGTACACGGAGATGGTGACCACTGGCGCGCTGCTGCATGGAGATGTGCCACGGCACCTGG
ATTTCAACGCCGCCGAGCACCCGGTGGCGCTGCAACTTGGCGGCAGCGAGCCAGCAGATCTCGCCGTAGCCGCTCGCCTT
GGCCAGCAGTGGGGCTATGCGGAAGTCAATCTGAATTGCGGATGCCCGTCCGAGCGCGTACAGCGTGGTGCATTCGGCGC
TTGCCTGATGGCCGAACCGCAACTCGTGGCCGACTGCGTGAAAGCCATGCGCGATGCCGTGTCGATTCCCGTTACGGTCA
AGCATCGCATCGGCATCGATAAGGTCGAGCATTACGGTTTCGTTCGGGATTTTGTCGGAACCGTGGCGCAGGCAGGCTGC
GAAACGTTTATCGTCCATGCACGCAATGCCATCCTGAAGGGGTTGAGCCCGAAGGAAAATCGCGAGATCCCGCCGCTCCG
CTATGAAGTTGCCTATCAGTTGAAGCAGGATTTCCCGCAACTGGAGATCCTGATCAACGGCGGCATCGTCAGCTATGACG
AAATGGCGAGCCACCTCGAGAATGTCGACGGCGTGATGATCGGCCGGGAGGCTTATCACCAGCCCTATGTGCTGGCGGAG
ATGGATGGCCGGTTCTACGGCGACGCGGAGGCGCCGGTGCGATCGCGGCTGGACGTGGAGCTAGCCATGCAGCGCTATAT
CGGCGAACTGGTGGAGCGCGGCGGTTACATGGGCGCCGTCACGCGCCACATGCTGGGCCTCTACCGCGGTGTGGCGGGTG
GCCGCGGCTGGCGGCGCGTGCTGTCAGACGCCAAGCGGATGTCGGCGGCCCGCACGCATGCCAATGTGGATGCGCTGTTC
GAAGAAGCGCGGTCCCATCTGCATGCTAACGCCCCGCTCGAACCCGAGCATCGCGCAGCCGCATAG

Upstream 100 bases:

>100_bases
CGTAGCGCTTGCACCCCAAAATGGCTTCAACGAGGTCAATTCCGAGACGACGAGACAGGGCGAGACCGCCCGCAGACCCT
AGTACTGACGCCGTTTTCCC

Downstream 100 bases:

>100_bases
GCGATTCGGAGGGGCGGCGCCAGAAAAGTCATCGAATAAATACTCTAATTCCGAATGGTGGATTTATGCATCAGGGCAGG
GCGGGTAGCGGGAGCGGCAC

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MKVSPRRISVAPMMDWTDRHCRTFHRQLSRHTWLYTEMVTTGALLHGDVPRHLDFNAAEHPVALQLGGSEPADLAVAARL
GQQWGYAEVNLNCGCPSERVQRGAFGACLMAEPQLVADCVKAMRDAVSIPVTVKHRIGIDKVEHYGFVRDFVGTVAQAGC
ETFIVHARNAILKGLSPKENREIPPLRYEVAYQLKQDFPQLEILINGGIVSYDEMASHLENVDGVMIGREAYHQPYVLAE
MDGRFYGDAEAPVRSRLDVELAMQRYIGELVERGGYMGAVTRHMLGLYRGVAGGRGWRRVLSDAKRMSAARTHANVDALF
EEARSHLHANAPLEPEHRAAA

Sequences:

>Translated_341_residues
MKVSPRRISVAPMMDWTDRHCRTFHRQLSRHTWLYTEMVTTGALLHGDVPRHLDFNAAEHPVALQLGGSEPADLAVAARL
GQQWGYAEVNLNCGCPSERVQRGAFGACLMAEPQLVADCVKAMRDAVSIPVTVKHRIGIDKVEHYGFVRDFVGTVAQAGC
ETFIVHARNAILKGLSPKENREIPPLRYEVAYQLKQDFPQLEILINGGIVSYDEMASHLENVDGVMIGREAYHQPYVLAE
MDGRFYGDAEAPVRSRLDVELAMQRYIGELVERGGYMGAVTRHMLGLYRGVAGGRGWRRVLSDAKRMSAARTHANVDALF
EEARSHLHANAPLEPEHRAAA
>Mature_341_residues
MKVSPRRISVAPMMDWTDRHCRTFHRQLSRHTWLYTEMVTTGALLHGDVPRHLDFNAAEHPVALQLGGSEPADLAVAARL
GQQWGYAEVNLNCGCPSERVQRGAFGACLMAEPQLVADCVKAMRDAVSIPVTVKHRIGIDKVEHYGFVRDFVGTVAQAGC
ETFIVHARNAILKGLSPKENREIPPLRYEVAYQLKQDFPQLEILINGGIVSYDEMASHLENVDGVMIGREAYHQPYVLAE
MDGRFYGDAEAPVRSRLDVELAMQRYIGELVERGGYMGAVTRHMLGLYRGVAGGRGWRRVLSDAKRMSAARTHANVDALF
EEARSHLHANAPLEPEHRAAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=262, Percent_Identity=27.0992366412214, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI145693211, Length=314, Percent_Identity=57.9617834394904, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI1788462, Length=243, Percent_Identity=29.2181069958848, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789660, Length=243, Percent_Identity=25.5144032921811, Blast_Score=76, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6323560, Length=241, Percent_Identity=26.1410788381743, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24580595, Length=244, Percent_Identity=26.6393442622951, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19920448, Length=244, Percent_Identity=26.6393442622951, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 37961; Mature: 37961

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVSPRRISVAPMMDWTDRHCRTFHRQLSRHTWLYTEMVTTGALLHGDVPRHLDFNAAEH
CCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PVALQLGGSEPADLAVAARLGQQWGYAEVNLNCGCPSERVQRGAFGACLMAEPQLVADCV
CEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHHCCCCCEEEECCHHHHHHHH
KAMRDAVSIPVTVKHRIGIDKVEHYGFVRDFVGTVAQAGCETFIVHARNAILKGLSPKEN
HHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCC
REIPPLRYEVAYQLKQDFPQLEILINGGIVSYDEMASHLENVDGVMIGREAYHQPYVLAE
CCCCCHHHHHHHHHHHCCCEEEEEEECCEECHHHHHHHHHCCCCEEECHHHHCCCEEEEE
MDGRFYGDAEAPVRSRLDVELAMQRYIGELVERGGYMGAVTRHMLGLYRGVAGGRGWRRV
CCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHH
LSDAKRMSAARTHANVDALFEEARSHLHANAPLEPEHRAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MKVSPRRISVAPMMDWTDRHCRTFHRQLSRHTWLYTEMVTTGALLHGDVPRHLDFNAAEH
CCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PVALQLGGSEPADLAVAARLGQQWGYAEVNLNCGCPSERVQRGAFGACLMAEPQLVADCV
CEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHHCCCCCEEEECCHHHHHHHH
KAMRDAVSIPVTVKHRIGIDKVEHYGFVRDFVGTVAQAGCETFIVHARNAILKGLSPKEN
HHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCC
REIPPLRYEVAYQLKQDFPQLEILINGGIVSYDEMASHLENVDGVMIGREAYHQPYVLAE
CCCCCHHHHHHHHHHHCCCEEEEEEECCEECHHHHHHHHHCCCCEEECHHHHCCCEEEEE
MDGRFYGDAEAPVRSRLDVELAMQRYIGELVERGGYMGAVTRHMLGLYRGVAGGRGWRRV
CCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHH
LSDAKRMSAARTHANVDALFEEARSHLHANAPLEPEHRAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]