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The map label for this gene is ansZ [H]

Identifier: 73541695

GI number: 73541695

Start: 2196027

End: 2197163

Strand: Reverse

Name: ansZ [H]

Synonym: Reut_A2007

Alternate gene names: 73541695

Gene position: 2197163-2196027 (Counterclockwise)

Preceding gene: 73541703

Following gene: 73541694

Centisome position: 57.72

GC content: 66.05

Gene sequence:

>1137_bases
ATGCCTGTGATTTTATACAGTATCGGCACAAGCGCAAGCAGAATTTCAGAGCCGTCTGCTCGACTTCGGGTTTGCCGGCA
TGCCGCAGGTAGAATCGGCGAACCTTTACTTCCTCGTGCCCTGCCCATGTCACGATCCATGTCCCAGCTTCCCCGCGTCG
TCATTCTGGCTACCGGCGGCACCATTGCCGGCGCTTCCGCCAATCCGGCCAGCAGTGCGCGCTATCAGGCTGCAACGGTG
CCGGTGACGACGCTGCTGGCTGCCGTGCCGGCGCTGCAATCCGTGGCCAGGGTCGAGGCCGAGCAGGTGGCGCAGGTCGA
CAGCAAGGACATGGTGTTCTCTCTATGGTCGACGCTGGCGGCGCGCGTGCAGCACTGGTCGGGCCAGCCGGACGTCGCGG
GTATTGTCATTACGCACGGCACCGACACGCTGGAAGAAACCGCCATGTTCCTGCACCTGACGCAGGCTTGCCCGGTGCCG
GTGGTGCTGACTGCGGCGATGCGGCCGTCGACCTCGCTGTCCGCCGATGGTCCGCTAAACCTGCTCGATGCGGTTCGGGT
TGCGGCCCATCCCGATGCCTGCGGCAAGGGCGTGCTGGTCGTGCTGAACCAGCAGATCCACGCGGCGCGCGACGTGGTCA
AGGCGCATACGTCCGCGGTAGATGCTTTCGTATCGGTACCATGCGGGCCGCTTGGCTTCGTGCAGGACGAGCTGGTGCGC
ATTGCCCGCGCGCCGGTGCGGTCTGGCGTGGGGGTGTTGCCAGTGCCGCAGCATTGGCCCGATGTGGAGATCATCGCCAG
CTATGCGCAACCTGGCCGCATGGTGGTGGATGCCCTCGTCGGCGCTGGTGTGCGGGGGATCGTCGTAGCGGCGGCGGGCA
ACGGCTCGGTACACGAGACGCTCGTGGCCGCGCTCAGGGATGCCGTGGCCGCTGGCGTCGCGGTGGTCCGCAGTTCGCGT
ACCGCGGCGGGTCATGTGGCGATTCCGCCAGTTGTTGATCCTCTCGGCGGGGCATTCCTTTCCGCCGCCGATCTCAATCC
CTATAAGGCCAGGGTCCTGCTGTTGCTGGCCCTGGCGCAGGATCCGGGGCTCGCTTCCGACCCGGAACGCCTGCAAGGGC
AGTTTGCCTCTTCGTGA

Upstream 100 bases:

>100_bases
CTGCTGCCGGGGTGTCAGGGTCGCCATGGAAACGCTTCCTTGATGAGTGCCCCGGTGCAATACGGCTGCCAGCCAGGCGG
GCACTGTATGTTTAAACAGT

Downstream 100 bases:

>100_bases
CAGCGGTTGCGCGTTTGTCAATAGCCGGGCTATAATTTAAGGCTATTCAACCTTGCCGCACCGGACGTGACGCCCGGGTG
GCTCATCCAAAAGGAGCGTC

Product: L-asparaginase, type II

Products: NA

Alternate protein names: L-ASNase 2; L-asparagine amidohydrolase 2 [H]

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MPVILYSIGTSASRISEPSARLRVCRHAAGRIGEPLLPRALPMSRSMSQLPRVVILATGGTIAGASANPASSARYQAATV
PVTTLLAAVPALQSVARVEAEQVAQVDSKDMVFSLWSTLAARVQHWSGQPDVAGIVITHGTDTLEETAMFLHLTQACPVP
VVLTAAMRPSTSLSADGPLNLLDAVRVAAHPDACGKGVLVVLNQQIHAARDVVKAHTSAVDAFVSVPCGPLGFVQDELVR
IARAPVRSGVGVLPVPQHWPDVEIIASYAQPGRMVVDALVGAGVRGIVVAAAGNGSVHETLVAALRDAVAAGVAVVRSSR
TAAGHVAIPPVVDPLGGAFLSAADLNPYKARVLLLLALAQDPGLASDPERLQGQFASS

Sequences:

>Translated_378_residues
MPVILYSIGTSASRISEPSARLRVCRHAAGRIGEPLLPRALPMSRSMSQLPRVVILATGGTIAGASANPASSARYQAATV
PVTTLLAAVPALQSVARVEAEQVAQVDSKDMVFSLWSTLAARVQHWSGQPDVAGIVITHGTDTLEETAMFLHLTQACPVP
VVLTAAMRPSTSLSADGPLNLLDAVRVAAHPDACGKGVLVVLNQQIHAARDVVKAHTSAVDAFVSVPCGPLGFVQDELVR
IARAPVRSGVGVLPVPQHWPDVEIIASYAQPGRMVVDALVGAGVRGIVVAAAGNGSVHETLVAALRDAVAAGVAVVRSSR
TAAGHVAIPPVVDPLGGAFLSAADLNPYKARVLLLLALAQDPGLASDPERLQGQFASS
>Mature_377_residues
PVILYSIGTSASRISEPSARLRVCRHAAGRIGEPLLPRALPMSRSMSQLPRVVILATGGTIAGASANPASSARYQAATVP
VTTLLAAVPALQSVARVEAEQVAQVDSKDMVFSLWSTLAARVQHWSGQPDVAGIVITHGTDTLEETAMFLHLTQACPVPV
VLTAAMRPSTSLSADGPLNLLDAVRVAAHPDACGKGVLVVLNQQIHAARDVVKAHTSAVDAFVSVPCGPLGFVQDELVRI
ARAPVRSGVGVLPVPQHWPDVEIIASYAQPGRMVVDALVGAGVRGIVVAAAGNGSVHETLVAALRDAVAAGVAVVRSSRT
AAGHVAIPPVVDPLGGAFLSAADLNPYKARVLLLLALAQDPGLASDPERLQGQFASS

Specific function: Catalyzes the conversion of L-asparagine to L-aspartate and ammonium [H]

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789327, Length=330, Percent_Identity=43.3333333333333, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI1788065, Length=292, Percent_Identity=27.0547945205479, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI193207374, Length=322, Percent_Identity=26.7080745341615, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17558278, Length=322, Percent_Identity=26.7080745341615, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17558280, Length=322, Percent_Identity=26.7080745341615, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI193207372, Length=325, Percent_Identity=26.4615384615385, Blast_Score=78, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6323189, Length=335, Percent_Identity=34.9253731343284, Blast_Score=170, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6323187, Length=335, Percent_Identity=34.9253731343284, Blast_Score=170, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6323186, Length=335, Percent_Identity=34.9253731343284, Blast_Score=170, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6323184, Length=335, Percent_Identity=34.9253731343284, Blast_Score=170, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6320527, Length=327, Percent_Identity=36.3914373088685, Blast_Score=164, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004550
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 38939; Mature: 38808

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVILYSIGTSASRISEPSARLRVCRHAAGRIGEPLLPRALPMSRSMSQLPRVVILATGG
CCEEEEECCCCHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCEEEEEEECC
TIAGASANPASSARYQAATVPVTTLLAAVPALQSVARVEAEQVAQVDSKDMVFSLWSTLA
EEECCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
ARVQHWSGQPDVAGIVITHGTDTLEETAMFLHLTQACPVPVVLTAAMRPSTSLSADGPLN
HHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCHH
LLDAVRVAAHPDACGKGVLVVLNQQIHAARDVVKAHTSAVDAFVSVPCGPLGFVQDELVR
HHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
IARAPVRSGVGVLPVPQHWPDVEIIASYAQPGRMVVDALVGAGVRGIVVAAAGNGSVHET
HHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHCCCCCEEEEEECCCCHHHHH
LVAALRDAVAAGVAVVRSSRTAAGHVAIPPVVDPLGGAFLSAADLNPYKARVLLLLALAQ
HHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCHHEECCCCCHHHHHHHHHHHHHC
DPGLASDPERLQGQFASS
CCCCCCCHHHHCCCCCCC
>Mature Secondary Structure 
PVILYSIGTSASRISEPSARLRVCRHAAGRIGEPLLPRALPMSRSMSQLPRVVILATGG
CEEEEECCCCHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCEEEEEEECC
TIAGASANPASSARYQAATVPVTTLLAAVPALQSVARVEAEQVAQVDSKDMVFSLWSTLA
EEECCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
ARVQHWSGQPDVAGIVITHGTDTLEETAMFLHLTQACPVPVVLTAAMRPSTSLSADGPLN
HHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCHH
LLDAVRVAAHPDACGKGVLVVLNQQIHAARDVVKAHTSAVDAFVSVPCGPLGFVQDELVR
HHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
IARAPVRSGVGVLPVPQHWPDVEIIASYAQPGRMVVDALVGAGVRGIVVAAAGNGSVHET
HHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHCCCCCEEEEEECCCCHHHHH
LVAALRDAVAAGVAVVRSSRTAAGHVAIPPVVDPLGGAFLSAADLNPYKARVLLLLALAQ
HHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCHHEECCCCCHHHHHHHHHHHHHC
DPGLASDPERLQGQFASS
CCCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274031; 9384377 [H]