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The map label for this gene is aceA [H]

Identifier: 73541649

GI number: 73541649

Start: 2147171

End: 2148466

Strand: Reverse

Name: aceA [H]

Synonym: Reut_A1961

Alternate gene names: 73541649

Gene position: 2148466-2147171 (Counterclockwise)

Preceding gene: 73541650

Following gene: 73541648

Centisome position: 56.44

GC content: 60.88

Gene sequence:

>1296_bases
ATGAACCGCGAACAACAAATCCAGGCTCTGCAAAAGGATTGGGATACCAATCCACGCTGGAAGGGCATCAAGCGCAACTT
CACGGCTGAAGACGTAGTGCGCCTGCGCGGTTCGGTCCAGGTGGAGCACACGCTGGCACGCCGCGGTTCCGAGAAACTGT
GGAACTTGCTGCATACAGAGCCGTTCGTGAACTCGCTGGGCGCACTGACCGGCAATCAGGCCATGCAGCAAGTCAAGGCC
GGCCTGAAGGCCATTTACCTGTCGGGCTGGCAAGTCGCTGGCGATGCCAATCTCGCCGGCGAGATGTACCCGGACCAGTC
GCTGTATCCGGCCAACTCGGTGCCGCAGGTCGTCAAGCGCATCAACAACACGTTCCAGCGCGCCGACCAGATCCAGTGGA
GCGAGGGCTCGGGCGACACCGATTTCTTCGCCCCGATCGTGGCTGACGCAGAAGCCGGCTTCGGCGGTGTGCTGAACGCG
TTCGAACTGATGAAGTCGATGATCGAAGCGGGCGCAGCCGGCGTGCACTTCGAAGACCAGCTCGCTTCGGTGAAGAAGTG
CGGGCACATGGGTGGCAAGGTACTGGTCCCGACTCGGGAAGCCGTGGCCAAGCTGACCGCGGCGCGTCTGGCTGCCGACG
TGTCGGGCGTTCCGACATTGGTGATTGCTCGTACCGATGCCGAAGCTGCTGACCTGCTTACCTCGGACGTAGACGACAAC
GACAAGCCGTTCTGCACTGGCGAGCGCACTGTGGAAGGCTTCTATCGCGTCCGCAACGGCCTGGAGCAGTCGATCTCGCG
GGCCCTGGCCTACGCTGAAGTTGCTGATCTGGTGTGGTGCGAAACCGGCAAGCCGGACCTCGAGTTCGCCAAGAAGTTTG
CCGAAGCCGTGCACGCCAAGTTCCCGGGCAAGATGCTGGCCTACAACTGCTCGCCGTCGTTCAACTGGAAGAAGAACCTC
GACGACGCGACCATCGCCAGGTTCCAAAGGGAGTTGGGCGCGATGGGCTACAAGTTCCAGTTCATCACACTGGCCGGTTT
CCACTCGCTGAACTACTCGATGTTTAACCTGGCCTACGGCTACGCCCGCAACCAGATGAGCGCGTTCGTGGAACTGCAGG
AAGCCGAGTTCAAGGCAGCCGAGAAAGGCTTCACCGCCGTGAAGCACCAGCGCGAAGTCGGCACCGGCTATTTCGACGCC
GTGACACAGGCGATCGAAGGCGGCCAGTCATCGACAACCGCATTGAAGGGATCTACCGAAGACGAGCAGTTCTTCGAAGA
CAAGAAAGTGGCCTGA

Upstream 100 bases:

>100_bases
GACCTGAGACGTAAAAGACGCGTCACCGCCGACAAGAGCGGACAGGTCAGGCATCACAGGTTTCCTTGCCATCCCCCAAT
TTGTCGACAGGAGAGTGAAC

Downstream 100 bases:

>100_bases
CTCTCCTGGCCACCGTTTGCTGAAACATAGATCGGGGGGAACCGAGCAGTCTCTAGGTTCAGCAGACCAGGGGGAGCGTG
CGACCATCACGCTCCTCTTT

Product: isocitrate lyase

Products: NA

Alternate protein names: ICL; Isocitrase; Isocitratase [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MNREQQIQALQKDWDTNPRWKGIKRNFTAEDVVRLRGSVQVEHTLARRGSEKLWNLLHTEPFVNSLGALTGNQAMQQVKA
GLKAIYLSGWQVAGDANLAGEMYPDQSLYPANSVPQVVKRINNTFQRADQIQWSEGSGDTDFFAPIVADAEAGFGGVLNA
FELMKSMIEAGAAGVHFEDQLASVKKCGHMGGKVLVPTREAVAKLTAARLAADVSGVPTLVIARTDAEAADLLTSDVDDN
DKPFCTGERTVEGFYRVRNGLEQSISRALAYAEVADLVWCETGKPDLEFAKKFAEAVHAKFPGKMLAYNCSPSFNWKKNL
DDATIARFQRELGAMGYKFQFITLAGFHSLNYSMFNLAYGYARNQMSAFVELQEAEFKAAEKGFTAVKHQREVGTGYFDA
VTQAIEGGQSSTTALKGSTEDEQFFEDKKVA

Sequences:

>Translated_431_residues
MNREQQIQALQKDWDTNPRWKGIKRNFTAEDVVRLRGSVQVEHTLARRGSEKLWNLLHTEPFVNSLGALTGNQAMQQVKA
GLKAIYLSGWQVAGDANLAGEMYPDQSLYPANSVPQVVKRINNTFQRADQIQWSEGSGDTDFFAPIVADAEAGFGGVLNA
FELMKSMIEAGAAGVHFEDQLASVKKCGHMGGKVLVPTREAVAKLTAARLAADVSGVPTLVIARTDAEAADLLTSDVDDN
DKPFCTGERTVEGFYRVRNGLEQSISRALAYAEVADLVWCETGKPDLEFAKKFAEAVHAKFPGKMLAYNCSPSFNWKKNL
DDATIARFQRELGAMGYKFQFITLAGFHSLNYSMFNLAYGYARNQMSAFVELQEAEFKAAEKGFTAVKHQREVGTGYFDA
VTQAIEGGQSSTTALKGSTEDEQFFEDKKVA
>Mature_431_residues
MNREQQIQALQKDWDTNPRWKGIKRNFTAEDVVRLRGSVQVEHTLARRGSEKLWNLLHTEPFVNSLGALTGNQAMQQVKA
GLKAIYLSGWQVAGDANLAGEMYPDQSLYPANSVPQVVKRINNTFQRADQIQWSEGSGDTDFFAPIVADAEAGFGGVLNA
FELMKSMIEAGAAGVHFEDQLASVKKCGHMGGKVLVPTREAVAKLTAARLAADVSGVPTLVIARTDAEAADLLTSDVDDN
DKPFCTGERTVEGFYRVRNGLEQSISRALAYAEVADLVWCETGKPDLEFAKKFAEAVHAKFPGKMLAYNCSPSFNWKKNL
DDATIARFQRELGAMGYKFQFITLAGFHSLNYSMFNLAYGYARNQMSAFVELQEAEFKAAEKGFTAVKHQREVGTGYFDA
VTQAIEGGQSSTTALKGSTEDEQFFEDKKVA

Specific function: Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle. May be involved in the assimilation of one-carbon compounds via the isocitrate lyase- positive serine pathway [H]

COG id: COG2224

COG function: function code C; Isocitrate lyase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1790445, Length=433, Percent_Identity=70.2078521939954, Blast_Score=624, Evalue=1e-180,
Organism=Escherichia coli, GI1786525, Length=253, Percent_Identity=29.6442687747036, Blast_Score=90, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71982926, Length=413, Percent_Identity=63.4382566585956, Blast_Score=554, Evalue=1e-158,
Organism=Caenorhabditis elegans, GI17561814, Length=413, Percent_Identity=63.4382566585956, Blast_Score=553, Evalue=1e-158,
Organism=Saccharomyces cerevisiae, GI6320908, Length=287, Percent_Identity=35.8885017421603, Blast_Score=168, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6325263, Length=251, Percent_Identity=37.4501992031873, Blast_Score=149, Evalue=8e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006254
- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.1 [H]

Molecular weight: Translated: 47422; Mature: 47422

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNREQQIQALQKDWDTNPRWKGIKRNFTAEDVVRLRGSVQVEHTLARRGSEKLWNLLHTE
CCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHCC
PFVNSLGALTGNQAMQQVKAGLKAIYLSGWQVAGDANLAGEMYPDQSLYPANSVPQVVKR
HHHHHHHHCCHHHHHHHHHHHHHEEEECCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHH
INNTFQRADQIQWSEGSGDTDFFAPIVADAEAGFGGVLNAFELMKSMIEAGAAGVHFEDQ
HHHHHHHHHCCEECCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHH
LASVKKCGHMGGKVLVPTREAVAKLTAARLAADVSGVPTLVIARTDAEAADLLTSDVDDN
HHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHCCCCCC
DKPFCTGERTVEGFYRVRNGLEQSISRALAYAEVADLVWCETGKPDLEFAKKFAEAVHAK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHC
FPGKMLAYNCSPSFNWKKNLDDATIARFQRELGAMGYKFQFITLAGFHSLNYSMFNLAYG
CCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECHHHCCHHHHHHHHH
YARNQMSAFVELQEAEFKAAEKGFTAVKHQREVGTGYFDAVTQAIEGGQSSTTALKGSTE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEECCCCC
DEQFFEDKKVA
HHHHHHHCCCC
>Mature Secondary Structure
MNREQQIQALQKDWDTNPRWKGIKRNFTAEDVVRLRGSVQVEHTLARRGSEKLWNLLHTE
CCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHCC
PFVNSLGALTGNQAMQQVKAGLKAIYLSGWQVAGDANLAGEMYPDQSLYPANSVPQVVKR
HHHHHHHHCCHHHHHHHHHHHHHEEEECCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHH
INNTFQRADQIQWSEGSGDTDFFAPIVADAEAGFGGVLNAFELMKSMIEAGAAGVHFEDQ
HHHHHHHHHCCEECCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHH
LASVKKCGHMGGKVLVPTREAVAKLTAARLAADVSGVPTLVIARTDAEAADLLTSDVDDN
HHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHCCCCCC
DKPFCTGERTVEGFYRVRNGLEQSISRALAYAEVADLVWCETGKPDLEFAKKFAEAVHAK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHC
FPGKMLAYNCSPSFNWKKNLDDATIARFQRELGAMGYKFQFITLAGFHSLNYSMFNLAYG
CCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECHHHCCHHHHHHHHH
YARNQMSAFVELQEAEFKAAEKGFTAVKHQREVGTGYFDAVTQAIEGGQSSTTALKGSTE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEECCCCC
DEQFFEDKKVA
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]