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The map label for this gene is yhcA [H]

Identifier: 73541630

GI number: 73541630

Start: 2128833

End: 2130314

Strand: Reverse

Name: yhcA [H]

Synonym: Reut_A1941

Alternate gene names: 73541630

Gene position: 2130314-2128833 (Counterclockwise)

Preceding gene: 73541631

Following gene: 73541628

Centisome position: 55.96

GC content: 68.02

Gene sequence:

>1482_bases
GTGAGCCGCGCTCCGCACGCGCCGGAAGATTCCGGCGGCGGGTCGGGCGGACCGACCCGGGCGGCGCTGCGCGCGCGCTA
CGGCGACCGCCTGCGCTGGCTGGTACTGCTCACGCTGATGCTTGGGACGATGTCGTCCATCGTCTCTTCCACCATCATCA
ACGTGGCTATTCCCGACCTGAGCCGCCATTTCGTGCTGGGACAGGAACGCGCACAATGGGTCGCGGCCAGTTTCATGATC
GCCATGACGCTGGCGATGCTGCTAACCCCGTGGCTGCTCAACCGGTACGGCCTGCGGCGGACGTTTCTGGGCGCGCTGGC
GCTGCTGGGCGTAGGCGGGGCGGTAGGCGGGCTCTCGCCGACCTACGGCGTGATGATCGCGATGCGCGTGGCGGAAGGCG
CCGCCGCGGGCATCATGCAGCCGCTGCCCAACATCCTCGTACTGCGTGTCTTCGACGAACGCGAGCAGGGCAAGGCCATG
AGCCTGTTCGGTTTCGGCGTGGTGCTGGCGCCCGCGCTGGGTCCGAGTCTGGGCGGTTTCCTTGTCGAGGCCTTCGGCTG
GCGCTCGATCTTCTTTGTGGTGGTTCCATTGACGCTCCTCGGCGCAGTCATGGCGCGTCGCTTCATGGCCGTCGACTCGA
TCATGATGGGCGAACGCAAGCCGCTGGACTGGCGCGGGCTAGCGCTGGCCGGTGTCGCCACGGTCTGCCTGTTGAACGGG
CTCGTGGAAATGCGCGAAAGCGTGGGCGCCGGGCTGGCGCTGGCCGGTCTGGGCGTGGTGCTGATCGTGGTGTTCGTGAT
GTGGCAGCTGCGCGCGGCCGATCCGCTGATGGACATGCGGCTCTACAGCTACCGCCAGTTCTCGGCGGGCGCGGTGGTCG
CGTTCATCTACGGCGCCGGCCTGTTCGGTTCGACCTACCTGCTGCCGGTGTACATGCAGATGGCGCTGAGCTATACGCCG
TCGCGCGCGGGCCTGGTGCTGCTGCCGGCCGGCATTGCGCTTGGGATTACGATCGCTGTGGCGGGACGCTTCACGCATCG
CGTATCGCCGCACTTGCAGGTGTCGTTGGGACTGGCGCTGCTGGCGCTGTCGTTCCTGCTGATGGCGACGGGTTCGCCGT
CCACGCCGTACCTGGTGCTGGTGGCGCTGGCGGTGCTGGGCCGAATCGGGCTCGGTTGCATCCTGCCGTCGCTGACGCTG
GGCTCCATGCGCGGTGTGGACTTCTCGCTGATTGCGCAAGGATCTAGCTGCATCAACTTCGTGCGCCAGCTTGGCGGGGC
GATCGGCGTCAGCCTGGCCGGCGTGGGCCTGCAATGGCGGCTGAACCAGCACGGCGTGGCGCTGGGGCAGGGCGGCGACG
TGCTGGCGCAAGCCGCGCGTATCCGCGCGTTCGATGAAACCTTCCTGGCCGTAGGCCTGGTGATCGCCACGGCCATTCTT
GCGTCCTGGCGCATCCGGCCGAAGCCGGTGCCAGGAGCTTGA

Upstream 100 bases:

>100_bases
GCGACGACCGCTACGCCGAGATCGTCAAATCCGCCTATCACGAGGCGGACATCGACACCGTACGCAAGATCCGCGAGATC
GCCGCCACGGGGCGCCTGGG

Downstream 100 bases:

>100_bases
GGCGGGGCGCCGTCTGCTGGAACGGACGGCGCCAGCGAAGCCTAGTTCGTCGCGCCGTTGCGCAGGTCTTCCACCAGCGC
GATGTAGCTCTCCATGGCCG

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MSRAPHAPEDSGGGSGGPTRAALRARYGDRLRWLVLLTLMLGTMSSIVSSTIINVAIPDLSRHFVLGQERAQWVAASFMI
AMTLAMLLTPWLLNRYGLRRTFLGALALLGVGGAVGGLSPTYGVMIAMRVAEGAAAGIMQPLPNILVLRVFDEREQGKAM
SLFGFGVVLAPALGPSLGGFLVEAFGWRSIFFVVVPLTLLGAVMARRFMAVDSIMMGERKPLDWRGLALAGVATVCLLNG
LVEMRESVGAGLALAGLGVVLIVVFVMWQLRAADPLMDMRLYSYRQFSAGAVVAFIYGAGLFGSTYLLPVYMQMALSYTP
SRAGLVLLPAGIALGITIAVAGRFTHRVSPHLQVSLGLALLALSFLLMATGSPSTPYLVLVALAVLGRIGLGCILPSLTL
GSMRGVDFSLIAQGSSCINFVRQLGGAIGVSLAGVGLQWRLNQHGVALGQGGDVLAQAARIRAFDETFLAVGLVIATAIL
ASWRIRPKPVPGA

Sequences:

>Translated_493_residues
MSRAPHAPEDSGGGSGGPTRAALRARYGDRLRWLVLLTLMLGTMSSIVSSTIINVAIPDLSRHFVLGQERAQWVAASFMI
AMTLAMLLTPWLLNRYGLRRTFLGALALLGVGGAVGGLSPTYGVMIAMRVAEGAAAGIMQPLPNILVLRVFDEREQGKAM
SLFGFGVVLAPALGPSLGGFLVEAFGWRSIFFVVVPLTLLGAVMARRFMAVDSIMMGERKPLDWRGLALAGVATVCLLNG
LVEMRESVGAGLALAGLGVVLIVVFVMWQLRAADPLMDMRLYSYRQFSAGAVVAFIYGAGLFGSTYLLPVYMQMALSYTP
SRAGLVLLPAGIALGITIAVAGRFTHRVSPHLQVSLGLALLALSFLLMATGSPSTPYLVLVALAVLGRIGLGCILPSLTL
GSMRGVDFSLIAQGSSCINFVRQLGGAIGVSLAGVGLQWRLNQHGVALGQGGDVLAQAARIRAFDETFLAVGLVIATAIL
ASWRIRPKPVPGA
>Mature_492_residues
SRAPHAPEDSGGGSGGPTRAALRARYGDRLRWLVLLTLMLGTMSSIVSSTIINVAIPDLSRHFVLGQERAQWVAASFMIA
MTLAMLLTPWLLNRYGLRRTFLGALALLGVGGAVGGLSPTYGVMIAMRVAEGAAAGIMQPLPNILVLRVFDEREQGKAMS
LFGFGVVLAPALGPSLGGFLVEAFGWRSIFFVVVPLTLLGAVMARRFMAVDSIMMGERKPLDWRGLALAGVATVCLLNGL
VEMRESVGAGLALAGLGVVLIVVFVMWQLRAADPLMDMRLYSYRQFSAGAVVAFIYGAGLFGSTYLLPVYMQMALSYTPS
RAGLVLLPAGIALGITIAVAGRFTHRVSPHLQVSLGLALLALSFLLMATGSPSTPYLVLVALAVLGRIGLGCILPSLTLG
SMRGVDFSLIAQGSSCINFVRQLGGAIGVSLAGVGLQWRLNQHGVALGQGGDVLAQAARIRAFDETFLAVGLVIATAILA
SWRIRPKPVPGA

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=425, Percent_Identity=28.4705882352941, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1788710, Length=413, Percent_Identity=27.3607748184019, Blast_Score=136, Evalue=4e-33,
Organism=Escherichia coli, GI1790195, Length=435, Percent_Identity=22.2988505747126, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788392, Length=460, Percent_Identity=26.5217391304348, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI87081983, Length=336, Percent_Identity=23.8095238095238, Blast_Score=86, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6323735, Length=318, Percent_Identity=25.7861635220126, Blast_Score=82, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 51873; Mature: 51741

Theoretical pI: Translated: 11.04; Mature: 11.04

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRAPHAPEDSGGGSGGPTRAALRARYGDRLRWLVLLTLMLGTMSSIVSSTIINVAIPDL
CCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SRHFVLGQERAQWVAASFMIAMTLAMLLTPWLLNRYGLRRTFLGALALLGVGGAVGGLSP
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
TYGVMIAMRVAEGAAAGIMQPLPNILVLRVFDEREQGKAMSLFGFGVVLAPALGPSLGGF
HHHHHHHHHHHCCHHHHHHHCCCCEEEEEEECCCCCCCEEEHHHHHHHHHHHCCCCHHHH
LVEAFGWRSIFFVVVPLTLLGAVMARRFMAVDSIMMGERKPLDWRGLALAGVATVCLLNG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
LVEMRESVGAGLALAGLGVVLIVVFVMWQLRAADPLMDMRLYSYRQFSAGAVVAFIYGAG
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHCC
LFGSTYLLPVYMQMALSYTPSRAGLVLLPAGIALGITIAVAGRFTHRVSPHLQVSLGLAL
HHCHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LALSFLLMATGSPSTPYLVLVALAVLGRIGLGCILPSLTLGSMRGVDFSLIAQGSSCINF
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHH
VRQLGGAIGVSLAGVGLQWRLNQHGVALGQGGDVLAQAARIRAFDETFLAVGLVIATAIL
HHHHHHHHHHHHCCCCEEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASWRIRPKPVPGA
HHCCCCCCCCCCC
>Mature Secondary Structure 
SRAPHAPEDSGGGSGGPTRAALRARYGDRLRWLVLLTLMLGTMSSIVSSTIINVAIPDL
CCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SRHFVLGQERAQWVAASFMIAMTLAMLLTPWLLNRYGLRRTFLGALALLGVGGAVGGLSP
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
TYGVMIAMRVAEGAAAGIMQPLPNILVLRVFDEREQGKAMSLFGFGVVLAPALGPSLGGF
HHHHHHHHHHHCCHHHHHHHCCCCEEEEEEECCCCCCCEEEHHHHHHHHHHHCCCCHHHH
LVEAFGWRSIFFVVVPLTLLGAVMARRFMAVDSIMMGERKPLDWRGLALAGVATVCLLNG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
LVEMRESVGAGLALAGLGVVLIVVFVMWQLRAADPLMDMRLYSYRQFSAGAVVAFIYGAG
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHCC
LFGSTYLLPVYMQMALSYTPSRAGLVLLPAGIALGITIAVAGRFTHRVSPHLQVSLGLAL
HHCHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LALSFLLMATGSPSTPYLVLVALAVLGRIGLGCILPSLTLGSMRGVDFSLIAQGSSCINF
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHH
VRQLGGAIGVSLAGVGLQWRLNQHGVALGQGGDVLAQAARIRAFDETFLAVGLVIATAIL
HHHHHHHHHHHHCCCCEEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASWRIRPKPVPGA
HHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 8969498 [H]