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The map label for this gene is mscM [H]

Identifier: 73541490

GI number: 73541490

Start: 1967248

End: 1968561

Strand: Reverse

Name: mscM [H]

Synonym: Reut_A1801

Alternate gene names: 73541490

Gene position: 1968561-1967248 (Counterclockwise)

Preceding gene: 73541491

Following gene: 73541488

Centisome position: 51.72

GC content: 65.14

Gene sequence:

>1314_bases
ATGGACTTCGATGCCCTGCCGGACCTGCTCCAGCGATACATGCCCACCCGGCCGTGGGCGCAGAACCTCGTCTACATGGC
GCTCCTCGTGCTGGTCGCGACCGTGGCGCAGTGGGTGGTGGCCAGGGTGGTGTCGCGCGTTGCCCACCGGCTGCTGGTGA
TCGCGCGCAATGGCCACTGGAGCAGGGCGCTCATGCGTCACCGGGTGTACCGGCGCTTTGGCTATGCGGTCTGGTTTGCC
ATCATCACCGTGGGCGCGGGCGAGGTCCCGAATATCGGTCGCTACGCGCAGGCTGTCGAAAGGGTGACGCATGCGGGCAC
GTGGATCTGCATCTTCACGATGCTGGCTGGCGCTCTCGGTGCCTGGCAGGACGTCTTTGCCGGCACCAGGCGCGCGCAGA
CGCGGTCGATCAAGGGCTATGTGCAGGTGGGCCGCCTGGGCTTGTGGCTGGTGTGCGGCGTACTGGTGCTGTCGATCCTG
ATCGACCGTTCGCCGCTGTGGATGCTGTCGGGCCTGGGTGCGCTGTCGGCAATCCTGCTGCTGGTGTTCAAGGACACGCT
GCTGTCGCTGGTGGCCAGCACGCAACTCACGTCGAACGACATGCTGCGCATCGGCGACTGGATCGAGATGCCGCAATGCA
ATGCCGACGGCTACGTCAAGGACATCGCGCTGCATACGGTCAAGGTGCAGAACTGGGACAACACCGTGACCACAGTGCCG
ACCTACAAGCTGTTTTCGGAGAGCTACCGCAACTATCGGCAGATGTTCGAGTCAGGCGCGCGCCGCATCAAGCGAACCTT
GCGTATCGACGCCGGCAGTGTGCGCTTCCTGAGCGATGATGAAGTCCACGCGCTGATGCGCTTCCGGCTGCTGCACGACT
ACCTGGAACAGAAGCAGTCCGAGGTCGACGAGGCCAACCGGCTGCTGATCGAAGCCGGGAGCGACCCGGTGAACCGCCGC
CGCCTGACCAATATCGGCACGTTCCGCGCCTATGGCATTGCCTACCTGAAGGCGCACCCGGAAATCCACCAGGAACTGCT
GCTCAACGTGCGCATGATGGAGGCAGATTCGCAGGGCATTCCGGTCGAGGTCTACTGCTTTACGCGCCTGACCGCGTGGA
TGGACTACGAACGCATCCAGGGCGACATCTTCGACCACCTGCTGGCGATCCTGCCCGAGCTGAGCCTCCGCCTGTACCAG
GCGCCGTCAGGCGCCGACCTGAGCGGCGTGCGCCTCAACCCCGTGCTGGCCGCAGCGCAGGTCGTGGCCGCACAGGCGGC
GCCGCCCGTGCAGCTATCCACGCCGATGCGGTAG

Upstream 100 bases:

>100_bases
CCGCGGCCTAGCCGTCACGGATTGCCGCCATGGCCGGGCGGGGGCTGGCCCGCACGTTTTGTACACTGTACGACTGAGTC
AGCCCCCAATTCCGCCATTC

Downstream 100 bases:

>100_bases
CCCTCGGCCTCGGCCTCAGCCCGCCACGCGCGGCTTGACGCACGCGGCTGCTTCCTTCGCGCGCTGGCGCGCGGTGTCCA
CGTCATCGGCATAGGCCAGC

Product: MscS mechanosensitive ion channel

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MDFDALPDLLQRYMPTRPWAQNLVYMALLVLVATVAQWVVARVVSRVAHRLLVIARNGHWSRALMRHRVYRRFGYAVWFA
IITVGAGEVPNIGRYAQAVERVTHAGTWICIFTMLAGALGAWQDVFAGTRRAQTRSIKGYVQVGRLGLWLVCGVLVLSIL
IDRSPLWMLSGLGALSAILLLVFKDTLLSLVASTQLTSNDMLRIGDWIEMPQCNADGYVKDIALHTVKVQNWDNTVTTVP
TYKLFSESYRNYRQMFESGARRIKRTLRIDAGSVRFLSDDEVHALMRFRLLHDYLEQKQSEVDEANRLLIEAGSDPVNRR
RLTNIGTFRAYGIAYLKAHPEIHQELLLNVRMMEADSQGIPVEVYCFTRLTAWMDYERIQGDIFDHLLAILPELSLRLYQ
APSGADLSGVRLNPVLAAAQVVAAQAAPPVQLSTPMR

Sequences:

>Translated_437_residues
MDFDALPDLLQRYMPTRPWAQNLVYMALLVLVATVAQWVVARVVSRVAHRLLVIARNGHWSRALMRHRVYRRFGYAVWFA
IITVGAGEVPNIGRYAQAVERVTHAGTWICIFTMLAGALGAWQDVFAGTRRAQTRSIKGYVQVGRLGLWLVCGVLVLSIL
IDRSPLWMLSGLGALSAILLLVFKDTLLSLVASTQLTSNDMLRIGDWIEMPQCNADGYVKDIALHTVKVQNWDNTVTTVP
TYKLFSESYRNYRQMFESGARRIKRTLRIDAGSVRFLSDDEVHALMRFRLLHDYLEQKQSEVDEANRLLIEAGSDPVNRR
RLTNIGTFRAYGIAYLKAHPEIHQELLLNVRMMEADSQGIPVEVYCFTRLTAWMDYERIQGDIFDHLLAILPELSLRLYQ
APSGADLSGVRLNPVLAAAQVVAAQAAPPVQLSTPMR
>Mature_437_residues
MDFDALPDLLQRYMPTRPWAQNLVYMALLVLVATVAQWVVARVVSRVAHRLLVIARNGHWSRALMRHRVYRRFGYAVWFA
IITVGAGEVPNIGRYAQAVERVTHAGTWICIFTMLAGALGAWQDVFAGTRRAQTRSIKGYVQVGRLGLWLVCGVLVLSIL
IDRSPLWMLSGLGALSAILLLVFKDTLLSLVASTQLTSNDMLRIGDWIEMPQCNADGYVKDIALHTVKVQNWDNTVTTVP
TYKLFSESYRNYRQMFESGARRIKRTLRIDAGSVRFLSDDEVHALMRFRLLHDYLEQKQSEVDEANRLLIEAGSDPVNRR
RLTNIGTFRAYGIAYLKAHPEIHQELLLNVRMMEADSQGIPVEVYCFTRLTAWMDYERIQGDIFDHLLAILPELSLRLYQ
APSGADLSGVRLNPVLAAAQVVAAQAAPPVQLSTPMR

Specific function: Mechanosensitive channel of miniconductance that confers protection against mild hypoosmotic shock. Overexpression confers protection against severe shocks [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786791, Length=382, Percent_Identity=41.3612565445026, Blast_Score=297, Evalue=1e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 49352; Mature: 49352

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFDALPDLLQRYMPTRPWAQNLVYMALLVLVATVAQWVVARVVSRVAHRLLVIARNGHW
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCH
SRALMRHRVYRRFGYAVWFAIITVGAGEVPNIGRYAQAVERVTHAGTWICIFTMLAGALG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHH
AWQDVFAGTRRAQTRSIKGYVQVGRLGLWLVCGVLVLSILIDRSPLWMLSGLGALSAILL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LVFKDTLLSLVASTQLTSNDMLRIGDWIEMPQCNADGYVKDIALHTVKVQNWDNTVTTVP
HHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCHHHHHHEEEEEEECCCCCEEECC
TYKLFSESYRNYRQMFESGARRIKRTLRIDAGSVRFLSDDEVHALMRFRLLHDYLEQKQS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHH
EVDEANRLLIEAGSDPVNRRRLTNIGTFRAYGIAYLKAHPEIHQELLLNVRMMEADSQGI
HHHHHCEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHEECCCCCC
PVEVYCFTRLTAWMDYERIQGDIFDHLLAILPELSLRLYQAPSGADLSGVRLNPVLAAAQ
CEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCEECHHHHHHH
VVAAQAAPPVQLSTPMR
HHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MDFDALPDLLQRYMPTRPWAQNLVYMALLVLVATVAQWVVARVVSRVAHRLLVIARNGHW
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCH
SRALMRHRVYRRFGYAVWFAIITVGAGEVPNIGRYAQAVERVTHAGTWICIFTMLAGALG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHH
AWQDVFAGTRRAQTRSIKGYVQVGRLGLWLVCGVLVLSILIDRSPLWMLSGLGALSAILL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LVFKDTLLSLVASTQLTSNDMLRIGDWIEMPQCNADGYVKDIALHTVKVQNWDNTVTTVP
HHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCHHHHHHEEEEEEECCCCCEEECC
TYKLFSESYRNYRQMFESGARRIKRTLRIDAGSVRFLSDDEVHALMRFRLLHDYLEQKQS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHH
EVDEANRLLIEAGSDPVNRRRLTNIGTFRAYGIAYLKAHPEIHQELLLNVRMMEADSQGI
HHHHHCEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHEECCCCCC
PVEVYCFTRLTAWMDYERIQGDIFDHLLAILPELSLRLYQAPSGADLSGVRLNPVLAAAQ
CEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCEECHHHHHHH
VVAAQAAPPVQLSTPMR
HHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 7813889 [H]