| Definition | Ralstonia eutropha JMP134 chromosome 1, complete sequence. |
|---|---|
| Accession | NC_007347 |
| Length | 3,806,533 |
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The map label for this gene is mrcA [H]
Identifier: 73541462
GI number: 73541462
Start: 1933566
End: 1935662
Strand: Reverse
Name: mrcA [H]
Synonym: Reut_A1773
Alternate gene names: 73541462
Gene position: 1935662-1933566 (Counterclockwise)
Preceding gene: 73541465
Following gene: 73541460
Centisome position: 50.85
GC content: 64.95
Gene sequence:
>2097_bases ATGCGCCGCCTTCGGCATCCGACGCGGCGCGGCATCCTCCTGGCCCTCGCCACTGTGCCGGCGCTGTTCCTGCTTTACGC GATCGCACTTGTGCCTTTCACCCCGAGCATCGGCGACATCCGCAAGGCGCGCATCGATCGTCCCGCGCTGATCGTGTCGG CCGATGGCAAGGTCATTGACGAGTTCAAGCCAGTCAATCGCGAATGGGTGCCGCTCGGGCAGATCTCGCCATACATGGTG GATGCATTGATCGCCACCGAAGACCGCCGCTTCTACGAGCACCACGGCATCGACTGGTTGCGGATCGCGTCAGCCGCGCT GCATACGTTCTCCGGTGATCGACAGGGCGGTTCGACCATCACGCAGCAACTGGCGCGCAACCTTTATCCCGACGAAATCG GCCGCGCACCGACGCTTGCGCGCAAACTCAGGGAAGCGATCACGGCGCTCAAGATCGAAACGGTATACAGCAAGGCGCAG ATCCTTGAGACATACCTGAACAATGTGCCCTTCCTGTACAACGCCTACGGCGTGGAAATGGCGGCGCGTACCTACTTTGG CAAGACAGCCCGCCAACTCGACATACTCGAAAGCGCGACACTCGTCGGCATGCTGAAGGCCAACAGCACCTACAACCCCG TGCTCAATCCCGAGCGTGCCTTGCAGCGGCGGAACACGGTGCTCGGGCAGATGAACAAGTACGGGACGCTAAAGCCCAGT GCCTACTCGTGGCTGAGCCGGCAAACGCTCAATGTCAATTTCCAGTTGCAGACCGCGTCACGAAGCGCGGCACCGCATTT TTCCGCGCAGCTGCGCCGGTGGCTGAGCGCGTGGGCGGATCGCAACGGCTACAACCTCTACGCTGACGGTCTCGTGGTGC GCACGACCCTGGACTCGCGCCTGCAGGCCATGGCGGTGCAGGCCATGGCCTGGCAGGCAAACCAGTTGCAATGGGTCGCG AACGGGGCATGGAACGAATACACGGGTTGCAGGCCAGGCAACGAGCCGTTCCGGACGTTCATGCGCCAAACCTCCGAGTA TCGCGCGGCACGCGATGCTGGCGAATCGGACCCGGCCGTGCTCAGGAAGCTCGGGACGAACCCGGCCTTCGTACGCGCCC TTTGCCGCAGCAAGGCGCAGGTGCAGGCAGGCTTTGTCGCGATCGATCCGCGTAGCGGCGATATCAAGGCATGGGTGGGC AGCCGCGATTTCAGCGACACGCCGTTCGATCACGTGCTGCAGGCCCGGCGCCAGCCGGGTTCAACCTTCAAGCCGTTTGT CTACGGGGCGGCGTTCGCGGACGGTGCACGCCCGAGCGACACGCTTTGGGACCGGAACGTTGCCATCCCAACGGCCGGGC AGGTGACCTGGCGGCCTACCGACGCCGAACCGCCGACCGACCAACCGATGTCGCTGCGCGACGCGCTGGTGTACTCGCGC AACCGCGTTACCGCCCAGCTCATGCTGAAGGAGGGGCCCGAGAAGGTCGCGCGGCTCGCGCGTGCGATGGGCGTGCGCGA CAGCGCATTGGAAGCGGTACCGTCGCTCGCACTCGGTACAAGTCCGGTCACACTCAAGGAAATGGTCTCGGCGTACAGCA CGATCGCGAACCGCGGCGTCTATCTGGAGCCGCGCATGGTGACTCGTATCGAGGACCACGACGGCAAGGTACTCGCTCAG TTTGCCAGCGCGGCGCCCGAACAGGCCTTGCCGGCTGGCGCGGCGCAAACGCTCGTCGATGTGATGCACGACGCAGTGGT TCGCGGCACCGGTGCGGCGATCCGGACGCGCTTCGGTATCCGCGCGGACGTGGCCGGCAAGACCGGCACGACGCAGGACA ACGCGGATGGCTGGTTCATCCTGATGCATCCGCAACTGGTGGCCGGCGCGTGGGTGGGATTTGACGACGGACGCGTGACG CTCGGCGACTACTGGGGCCAAGGCGCGCGCAGCGCCCTGCCGATGGTGGGCGCGTTCTACGATATGGCGCTACGGGGGCG AGTGATCGATTCCGATGCGCGATTCAACCCTGAGAGCCGGCCGCCTCGCAAGGCCCGTCCGCCACGGCATCACCGCTTCC TGTTCTGGAACTTCTGA
Upstream 100 bases:
>100_bases TGTATCGTTGTTTCGTGAGTATTGCGCGCGGGGGCTCGTCCACATCCGCCCGCTGGCCAGAGCCGGCATCGCGTTAGCCA GTCGCGCGCTGGGGGGCGCG
Downstream 100 bases:
>100_bases TGCCGTGGCTGGCGGCGGGCACTAGCGCCGTGGTGCCCGGTCGCGTTGCTCAGTCGTCGTGGCGATGCTTCTTCTGGCCC TTGTGCTTGCCGCGATTGCC
Product: glycosyl transferase family penicillin-binding protein transpeptidase
Products: NA
Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]
Number of amino acids: Translated: 698; Mature: 698
Protein sequence:
>698_residues MRRLRHPTRRGILLALATVPALFLLYAIALVPFTPSIGDIRKARIDRPALIVSADGKVIDEFKPVNREWVPLGQISPYMV DALIATEDRRFYEHHGIDWLRIASAALHTFSGDRQGGSTITQQLARNLYPDEIGRAPTLARKLREAITALKIETVYSKAQ ILETYLNNVPFLYNAYGVEMAARTYFGKTARQLDILESATLVGMLKANSTYNPVLNPERALQRRNTVLGQMNKYGTLKPS AYSWLSRQTLNVNFQLQTASRSAAPHFSAQLRRWLSAWADRNGYNLYADGLVVRTTLDSRLQAMAVQAMAWQANQLQWVA NGAWNEYTGCRPGNEPFRTFMRQTSEYRAARDAGESDPAVLRKLGTNPAFVRALCRSKAQVQAGFVAIDPRSGDIKAWVG SRDFSDTPFDHVLQARRQPGSTFKPFVYGAAFADGARPSDTLWDRNVAIPTAGQVTWRPTDAEPPTDQPMSLRDALVYSR NRVTAQLMLKEGPEKVARLARAMGVRDSALEAVPSLALGTSPVTLKEMVSAYSTIANRGVYLEPRMVTRIEDHDGKVLAQ FASAAPEQALPAGAAQTLVDVMHDAVVRGTGAAIRTRFGIRADVAGKTGTTQDNADGWFILMHPQLVAGAWVGFDDGRVT LGDYWGQGARSALPMVGAFYDMALRGRVIDSDARFNPESRPPRKARPPRHHRFLFWNF
Sequences:
>Translated_698_residues MRRLRHPTRRGILLALATVPALFLLYAIALVPFTPSIGDIRKARIDRPALIVSADGKVIDEFKPVNREWVPLGQISPYMV DALIATEDRRFYEHHGIDWLRIASAALHTFSGDRQGGSTITQQLARNLYPDEIGRAPTLARKLREAITALKIETVYSKAQ ILETYLNNVPFLYNAYGVEMAARTYFGKTARQLDILESATLVGMLKANSTYNPVLNPERALQRRNTVLGQMNKYGTLKPS AYSWLSRQTLNVNFQLQTASRSAAPHFSAQLRRWLSAWADRNGYNLYADGLVVRTTLDSRLQAMAVQAMAWQANQLQWVA NGAWNEYTGCRPGNEPFRTFMRQTSEYRAARDAGESDPAVLRKLGTNPAFVRALCRSKAQVQAGFVAIDPRSGDIKAWVG SRDFSDTPFDHVLQARRQPGSTFKPFVYGAAFADGARPSDTLWDRNVAIPTAGQVTWRPTDAEPPTDQPMSLRDALVYSR NRVTAQLMLKEGPEKVARLARAMGVRDSALEAVPSLALGTSPVTLKEMVSAYSTIANRGVYLEPRMVTRIEDHDGKVLAQ FASAAPEQALPAGAAQTLVDVMHDAVVRGTGAAIRTRFGIRADVAGKTGTTQDNADGWFILMHPQLVAGAWVGFDDGRVT LGDYWGQGARSALPMVGAFYDMALRGRVIDSDARFNPESRPPRKARPPRHHRFLFWNF >Mature_698_residues MRRLRHPTRRGILLALATVPALFLLYAIALVPFTPSIGDIRKARIDRPALIVSADGKVIDEFKPVNREWVPLGQISPYMV DALIATEDRRFYEHHGIDWLRIASAALHTFSGDRQGGSTITQQLARNLYPDEIGRAPTLARKLREAITALKIETVYSKAQ ILETYLNNVPFLYNAYGVEMAARTYFGKTARQLDILESATLVGMLKANSTYNPVLNPERALQRRNTVLGQMNKYGTLKPS AYSWLSRQTLNVNFQLQTASRSAAPHFSAQLRRWLSAWADRNGYNLYADGLVVRTTLDSRLQAMAVQAMAWQANQLQWVA NGAWNEYTGCRPGNEPFRTFMRQTSEYRAARDAGESDPAVLRKLGTNPAFVRALCRSKAQVQAGFVAIDPRSGDIKAWVG SRDFSDTPFDHVLQARRQPGSTFKPFVYGAAFADGARPSDTLWDRNVAIPTAGQVTWRPTDAEPPTDQPMSLRDALVYSR NRVTAQLMLKEGPEKVARLARAMGVRDSALEAVPSLALGTSPVTLKEMVSAYSTIANRGVYLEPRMVTRIEDHDGKVLAQ FASAAPEQALPAGAAQTLVDVMHDAVVRGTGAAIRTRFGIRADVAGKTGTTQDNADGWFILMHPQLVAGAWVGFDDGRVT LGDYWGQGARSALPMVGAFYDMALRGRVIDSDARFNPESRPPRKARPPRHHRFLFWNF
Specific function: Cell Wall Formation. Synthesis Of Cross-Linked Peptidoglycan From The Lipid Intermediates. The Enzyme Has A Penicillin-Insensitive Transglycosylase N-Terminal Domain (Formation Of Linear Glycan Strands) And A Penicillin-Sensitive Transpeptidase C-Terminal
COG id: COG5009
COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein
Gene ontology:
Cell location: Cell inner membrane; Single-pass type II membrane protein [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: In the C-terminal section; belongs to the transpeptidase family [H]
Homologues:
Organism=Escherichia coli, GI87082258, Length=793, Percent_Identity=28.8776796973518, Blast_Score=259, Evalue=3e-70, Organism=Escherichia coli, GI1786343, Length=584, Percent_Identity=30.6506849315068, Blast_Score=192, Evalue=6e-50, Organism=Escherichia coli, GI1788867, Length=235, Percent_Identity=34.0425531914894, Blast_Score=106, Evalue=4e-24, Organism=Escherichia coli, GI1789601, Length=235, Percent_Identity=36.1702127659575, Blast_Score=99, Evalue=1e-21,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR012338 - InterPro: IPR001264 - InterPro: IPR011816 - InterPro: IPR001460 [H]
Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]
EC number: 2.4.2.-; 3.4.-.- [C]
Molecular weight: Translated: 77475; Mature: 77475
Theoretical pI: Translated: 10.36; Mature: 10.36
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.3 %Cys (Translated Protein) 2.4 %Met (Translated Protein) 2.7 %Cys+Met (Translated Protein) 0.3 %Cys (Mature Protein) 2.4 %Met (Mature Protein) 2.7 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MRRLRHPTRRGILLALATVPALFLLYAIALVPFTPSIGDIRKARIDRPALIVSADGKVID CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEECCCCCHH EFKPVNREWVPLGQISPYMVDALIATEDRRFYEHHGIDWLRIASAALHTFSGDRQGGSTI HCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHH TQQLARNLYPDEIGRAPTLARKLREAITALKIETVYSKAQILETYLNNVPFLYNAYGVEM HHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHH AARTYFGKTARQLDILESATLVGMLKANSTYNPVLNPERALQRRNTVLGQMNKYGTLKPS HHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCH AYSWLSRQTLNVNFQLQTASRSAAPHFSAQLRRWLSAWADRNGYNLYADGLVVRTTLDSR HHHHHCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCEEEEECHHHH LQAMAVQAMAWQANQLQWVANGAWNEYTGCRPGNEPFRTFMRQTSEYRAARDAGESDPAV HHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHH LRKLGTNPAFVRALCRSKAQVQAGFVAIDPRSGDIKAWVGSRDFSDTPFDHVLQARRQPG HHHHCCCHHHHHHHHHHHHHEEECEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHCCCC STFKPFVYGAAFADGARPSDTLWDRNVAIPTAGQVTWRPTDAEPPTDQPMSLRDALVYSR CCCCCHHEEEHHCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHC NRVTAQLMLKEGPEKVARLARAMGVRDSALEAVPSLALGTSPVTLKEMVSAYSTIANRGV CCCEEEHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCE YLEPRMVTRIEDHDGKVLAQFASAAPEQALPAGAAQTLVDVMHDAVVRGTGAAIRTRFGI EECCHHEEEEECCCCHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCC RADVAGKTGTTQDNADGWFILMHPQLVAGAWVGFDDGRVTLGDYWGQGARSALPMVGAFY EEECCCCCCCCCCCCCEEEEEECCHHHHHEEEECCCCEEEECHHCCCCHHHHHHHHHHHH DMALRGRVIDSDARFNPESRPPRKARPPRHHRFLFWNF HHHHCCEEECCCCCCCCCCCCCCCCCCCCCCEEEEECC >Mature Secondary Structure MRRLRHPTRRGILLALATVPALFLLYAIALVPFTPSIGDIRKARIDRPALIVSADGKVID CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEECCCCCHH EFKPVNREWVPLGQISPYMVDALIATEDRRFYEHHGIDWLRIASAALHTFSGDRQGGSTI HCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHH TQQLARNLYPDEIGRAPTLARKLREAITALKIETVYSKAQILETYLNNVPFLYNAYGVEM HHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHH AARTYFGKTARQLDILESATLVGMLKANSTYNPVLNPERALQRRNTVLGQMNKYGTLKPS HHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCH AYSWLSRQTLNVNFQLQTASRSAAPHFSAQLRRWLSAWADRNGYNLYADGLVVRTTLDSR HHHHHCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCEEEEECHHHH LQAMAVQAMAWQANQLQWVANGAWNEYTGCRPGNEPFRTFMRQTSEYRAARDAGESDPAV HHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHH LRKLGTNPAFVRALCRSKAQVQAGFVAIDPRSGDIKAWVGSRDFSDTPFDHVLQARRQPG HHHHCCCHHHHHHHHHHHHHEEECEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHCCCC STFKPFVYGAAFADGARPSDTLWDRNVAIPTAGQVTWRPTDAEPPTDQPMSLRDALVYSR CCCCCHHEEEHHCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHC NRVTAQLMLKEGPEKVARLARAMGVRDSALEAVPSLALGTSPVTLKEMVSAYSTIANRGV CCCEEEHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCE YLEPRMVTRIEDHDGKVLAQFASAAPEQALPAGAAQTLVDVMHDAVVRGTGAAIRTRFGI EECCHHEEEEECCCCHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCC RADVAGKTGTTQDNADGWFILMHPQLVAGAWVGFDDGRVTLGDYWGQGARSALPMVGAFY EEECCCCCCCCCCCCCEEEEEECCHHHHHEEEECCCCEEEECHHCCCCHHHHHHHHHHHH DMALRGRVIDSDARFNPESRPPRKARPPRHHRFLFWNF HHHHCCEEECCCCCCCCCCCCCCCCCCCCCCEEEEECC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 9537320 [H]