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The map label for this gene is cysL [H]

Identifier: 73541322

GI number: 73541322

Start: 1754533

End: 1755435

Strand: Reverse

Name: cysL [H]

Synonym: Reut_A1632

Alternate gene names: 73541322

Gene position: 1755435-1754533 (Counterclockwise)

Preceding gene: 73541324

Following gene: 73541321

Centisome position: 46.12

GC content: 59.69

Gene sequence:

>903_bases
ATGAGATTCGATCTCGCGGATCTGCGCTTGTTCGTTTGCGTGGTCGATGCCGGCAGCATCACAGCCGGGGCCAGACAAGC
AAACCTTGCGTTGGCGTCGGCAAGCGAGCGTCTCAGCAATATCGAGGCGGATGCCGGTGTCGTCCTGCTTGAGAGAAGCC
GCAAAGGGGTAGTAACCACCGAGGCAGGCGAGGCGCTGGCGCACCATGCCCGTCTGATCCTCCGACAGCACAGCCTGTTG
CAAGGGGAGCTACGGGACTTTGGTGCTGGTGCACGCGGTACGTTGCAGCTGTACGCAAATACGGCGGCGCTGACTGGCCT
GTTGCCGGAGAAGCTTGCCCCGTGGCTTGCACAGCGTCCGCGTCTGCACATGGATCTCAGAGAACGTACAAGTGCCGAGA
TCGTGAAGATGATCAGCGCGGGACTGGCCGAAGCGGGCGTGGTATCCGATGCCGTCAATCCCACGGGTCTCACAATCCAG
CCCGTCGGCAGCGACCATCTCGTCCTGATTGTTCCTGTGGCACACAGACTCGCAGCATCGAAGGAAGTTTGCTTTGCCGA
AATTACGGGCGAGCAGTTCGTTGGCCTGGAGGCGGGGAGCGCGCTTCAAGACCACATCGAAGAACACGCCGCAGGTCTGC
GTAAGACGTTGTCACTCCGTATTCGCATGAAGACCTTCGAGGGATCGTGCCAGATGGTGTCGCAGGGAGTGGGAGTCGCG
ATCATTCCGTTGGCGGCCGCAAAACGCTATCGACGCAGATATCCGTTCAAAACCCTCATGATCAGAGAGGATTGGGCGCG
CAGACAGCTCTGCCTGTGCTTTCAGCAATGGCACAGTCTGTCTAAGCCGATGCAAAGTCTGCTGGCGCATCTCGGTGGTA
GACCGCAGACCCCCTCACTGTGA

Upstream 100 bases:

>100_bases
AGTCATAGCGGCCATTACATATCGGGTCCTGGGCTGGCACAATGCGGTTTTTCCGACACCCCCTTCGGATTTGCCGAAGG
AAAGCCGTCTTGGAGGCCGT

Downstream 100 bases:

>100_bases
ACAATGTGACAACACAAGGCGACCGGTCCGCTACGGCAATCGATACTCCATCCGTATGGTTGCAGACCCAATCAGTATTG
GACTGAGCCCCGCCGCCGCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: CysJI operon transcriptional activator [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MRFDLADLRLFVCVVDAGSITAGARQANLALASASERLSNIEADAGVVLLERSRKGVVTTEAGEALAHHARLILRQHSLL
QGELRDFGAGARGTLQLYANTAALTGLLPEKLAPWLAQRPRLHMDLRERTSAEIVKMISAGLAEAGVVSDAVNPTGLTIQ
PVGSDHLVLIVPVAHRLAASKEVCFAEITGEQFVGLEAGSALQDHIEEHAAGLRKTLSLRIRMKTFEGSCQMVSQGVGVA
IIPLAAAKRYRRRYPFKTLMIREDWARRQLCLCFQQWHSLSKPMQSLLAHLGGRPQTPSL

Sequences:

>Translated_300_residues
MRFDLADLRLFVCVVDAGSITAGARQANLALASASERLSNIEADAGVVLLERSRKGVVTTEAGEALAHHARLILRQHSLL
QGELRDFGAGARGTLQLYANTAALTGLLPEKLAPWLAQRPRLHMDLRERTSAEIVKMISAGLAEAGVVSDAVNPTGLTIQ
PVGSDHLVLIVPVAHRLAASKEVCFAEITGEQFVGLEAGSALQDHIEEHAAGLRKTLSLRIRMKTFEGSCQMVSQGVGVA
IIPLAAAKRYRRRYPFKTLMIREDWARRQLCLCFQQWHSLSKPMQSLLAHLGGRPQTPSL
>Mature_300_residues
MRFDLADLRLFVCVVDAGSITAGARQANLALASASERLSNIEADAGVVLLERSRKGVVTTEAGEALAHHARLILRQHSLL
QGELRDFGAGARGTLQLYANTAALTGLLPEKLAPWLAQRPRLHMDLRERTSAEIVKMISAGLAEAGVVSDAVNPTGLTIQ
PVGSDHLVLIVPVAHRLAASKEVCFAEITGEQFVGLEAGSALQDHIEEHAAGLRKTLSLRIRMKTFEGSCQMVSQGVGVA
IIPLAAAKRYRRRYPFKTLMIREDWARRQLCLCFQQWHSLSKPMQSLLAHLGGRPQTPSL

Specific function: Transcriptional activator of the cysJI operon which is involved in sulfur assimilation. Also negatively regulates its own transcription [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI145693105, Length=243, Percent_Identity=22.2222222222222, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787879, Length=298, Percent_Identity=24.496644295302, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1790399, Length=274, Percent_Identity=23.3576642335766, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI157672245, Length=245, Percent_Identity=22.0408163265306, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787806, Length=290, Percent_Identity=21.7241379310345, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32617; Mature: 32617

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFDLADLRLFVCVVDAGSITAGARQANLALASASERLSNIEADAGVVLLERSRKGVVTT
CCCCHHHHEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEE
EAGEALAHHARLILRQHSLLQGELRDFGAGARGTLQLYANTAALTGLLPEKLAPWLAQRP
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHHHHCCHHHHHHHHHCCC
RLHMDLRERTSAEIVKMISAGLAEAGVVSDAVNPTGLTIQPVGSDHLVLIVPVAHRLAAS
CCCCCHHHHHHHHHHHHHHHCHHHCCHHHCCCCCCCCEEEECCCCCEEEEEEHHHHHHHC
KEVCFAEITGEQFVGLEAGSALQDHIEEHAAGLRKTLSLRIRMKTFEGSCQMVSQGVGVA
CCEEEEEECCCCEECCCCCHHHHHHHHHHHHHHHHHHEEEEEEEECCCCHHHHHCCCCEE
IIPLAAAKRYRRRYPFKTLMIREDWARRQLCLCFQQWHSLSKPMQSLLAHLGGRPQTPSL
EEHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRFDLADLRLFVCVVDAGSITAGARQANLALASASERLSNIEADAGVVLLERSRKGVVTT
CCCCHHHHEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEE
EAGEALAHHARLILRQHSLLQGELRDFGAGARGTLQLYANTAALTGLLPEKLAPWLAQRP
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHHHHCCHHHHHHHHHCCC
RLHMDLRERTSAEIVKMISAGLAEAGVVSDAVNPTGLTIQPVGSDHLVLIVPVAHRLAAS
CCCCCHHHHHHHHHHHHHHHCHHHCCHHHCCCCCCCCEEEECCCCCEEEEEEHHHHHHHC
KEVCFAEITGEQFVGLEAGSALQDHIEEHAAGLRKTLSLRIRMKTFEGSCQMVSQGVGVA
CCEEEEEECCCCEECCCCCHHHHHHHHHHHHHHHHHHEEEEEEEECCCCHHHHHCCCCEE
IIPLAAAKRYRRRYPFKTLMIREDWARRQLCLCFQQWHSLSKPMQSLLAHLGGRPQTPSL
EEHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]