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The map label for this gene is fadH [C]

Identifier: 73541265

GI number: 73541265

Start: 1697238

End: 1698539

Strand: Direct

Name: fadH [C]

Synonym: Reut_A1575

Alternate gene names: 73541265

Gene position: 1697238-1698539 (Clockwise)

Preceding gene: 73541264

Following gene: 73541266

Centisome position: 44.59

GC content: 67.59

Gene sequence:

>1302_bases
ATGGAGGCTCCGCAATCCGCCGGCGCAGCGGCATTCGCACCGGTTCGCATCGGCCCGCTGACGCTGCGCAACCGCTTTAT
CAAGGCTGGCGCAAACGAGGGCATGACACCCGAAGGCCTGCCGACGCAGGCGCTGGTCAAGCATCACGCCGACCTGGCCG
CAGGTGGCGTAGGCATGACCACGGTCGCCTATGCCGCCGTGGCCGATGACGGCTTGACCTTCGCGCACCAGCTCAGCATG
CGCGCGGAGCAGGTGCCGCATTTGCGCGTACTGACCGATGCCGTGCACCGCGAGGGCGCTGCGGCCTGTCTGCAGATTAC
CCATGCCGGCTCGTTCACCACCATGCGCCACGGCGGCAGCCGTGCGCCGGGCAGCGCGTCGACGGGGCTCAATGCCTTCG
GCATGATGCACGGCGTGTATTTCCAGCGCGCCATGAGCCAGGCGGACATGTCGCGCCGCGCCGCGCAGTTCGCACAGGCG
GCGCGGCTCGCGCGCGAGGCCGGCTTCGACGCCGTGGAAATCCATATGGGCCATGGCTACCTGCTTAACCAGTTTCTGTC
GCCGCTAAGCAACCGGCGTCGCGACATGTTTGGCGGCAGCGTGGAAAACCGCACGCGCTTCCCGGCGATGGTGCTGCGCC
AGGTAAAGGATGCAGTCGGTGCCGAGATGGCGGTCTGCTGCAAGCTCAGCGTGACCGACGGCGTGCCCGGCGGCAATGAC
GCTGCCGACTCCGCAATCACCGCGCGCATCCTGGAAGCGGAGGGCGCCGACCTGCTGACCCTCAGCGGCGGACGCAATGT
CGAAAGCCCGTGGGCGCTGTTCGGCAGCCCGATGCCGACCGCGCAGATGAAGGCTGCAGCGCCGAACGCAATGGCCCGCT
TCGGCATCACCATGCTGGAGAAGCGCACGCCGCGCGACCTGACATTCCGGGAACTGTATTTCCTCGAGGCGTCGCGCGTG
GTGCGGCAGGCGGTGCAAATGCCGCTGGCCTATATCGGCGGCGTGAAATCGCTGCAGAACGTGGATGCATTGCTCGGCGA
AGGCTTCGACTGCATCGCGCTGGCGCGCGCGCTGATCCACGATCCGGCGCTGGTCGAGCACTGGCGTAACGGCGTGGCCG
CGCGTTCGGCGTGCGACAGCTGCAATGGCTGCGTGGCAAAGATCTACGACCCGGCGGGCGTGTGCTGCGTGCACGGCCCG
GCGAACGATCCCGCGCTCACCGGCATCCCGGCGGCGTTGAAGCCGATCCAGGTCCTGAAGTCCGTGAAGGCGCACACAGA
GCCTAGTCTGATGGGACGATGA

Upstream 100 bases:

>100_bases
ATGTCGATGGTCTTCTGCGCTGTCCCACGAACGGGCAGAGCAGAAGACCATCGGTTCATATGAGTCATTACCAAAAGCAA
AAGACCCAGGGAGGGACAGC

Downstream 100 bases:

>100_bases
GCGGCGTGCGCGCGCCTCGTATCGTGAAGCATCCACAGAGATCCAAGGAGACTGACGCCATGACAACTACCGGCTACAAG
ATCGAAACGCGCCCGCTCGA

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MEAPQSAGAAAFAPVRIGPLTLRNRFIKAGANEGMTPEGLPTQALVKHHADLAAGGVGMTTVAYAAVADDGLTFAHQLSM
RAEQVPHLRVLTDAVHREGAAACLQITHAGSFTTMRHGGSRAPGSASTGLNAFGMMHGVYFQRAMSQADMSRRAAQFAQA
ARLAREAGFDAVEIHMGHGYLLNQFLSPLSNRRRDMFGGSVENRTRFPAMVLRQVKDAVGAEMAVCCKLSVTDGVPGGND
AADSAITARILEAEGADLLTLSGGRNVESPWALFGSPMPTAQMKAAAPNAMARFGITMLEKRTPRDLTFRELYFLEASRV
VRQAVQMPLAYIGGVKSLQNVDALLGEGFDCIALARALIHDPALVEHWRNGVAARSACDSCNGCVAKIYDPAGVCCVHGP
ANDPALTGIPAALKPIQVLKSVKAHTEPSLMGR

Sequences:

>Translated_433_residues
MEAPQSAGAAAFAPVRIGPLTLRNRFIKAGANEGMTPEGLPTQALVKHHADLAAGGVGMTTVAYAAVADDGLTFAHQLSM
RAEQVPHLRVLTDAVHREGAAACLQITHAGSFTTMRHGGSRAPGSASTGLNAFGMMHGVYFQRAMSQADMSRRAAQFAQA
ARLAREAGFDAVEIHMGHGYLLNQFLSPLSNRRRDMFGGSVENRTRFPAMVLRQVKDAVGAEMAVCCKLSVTDGVPGGND
AADSAITARILEAEGADLLTLSGGRNVESPWALFGSPMPTAQMKAAAPNAMARFGITMLEKRTPRDLTFRELYFLEASRV
VRQAVQMPLAYIGGVKSLQNVDALLGEGFDCIALARALIHDPALVEHWRNGVAARSACDSCNGCVAKIYDPAGVCCVHGP
ANDPALTGIPAALKPIQVLKSVKAHTEPSLMGR
>Mature_433_residues
MEAPQSAGAAAFAPVRIGPLTLRNRFIKAGANEGMTPEGLPTQALVKHHADLAAGGVGMTTVAYAAVADDGLTFAHQLSM
RAEQVPHLRVLTDAVHREGAAACLQITHAGSFTTMRHGGSRAPGSASTGLNAFGMMHGVYFQRAMSQADMSRRAAQFAQA
ARLAREAGFDAVEIHMGHGYLLNQFLSPLSNRRRDMFGGSVENRTRFPAMVLRQVKDAVGAEMAVCCKLSVTDGVPGGND
AADSAITARILEAEGADLLTLSGGRNVESPWALFGSPMPTAQMKAAAPNAMARFGITMLEKRTPRDLTFRELYFLEASRV
VRQAVQMPLAYIGGVKSLQNVDALLGEGFDCIALARALIHDPALVEHWRNGVAARSACDSCNGCVAKIYDPAGVCCVHGP
ANDPALTGIPAALKPIQVLKSVKAHTEPSLMGR

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=398, Percent_Identity=28.1407035175879, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1787939, Length=377, Percent_Identity=28.3819628647215, Blast_Score=103, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17564188, Length=382, Percent_Identity=25.6544502617801, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17559804, Length=387, Percent_Identity=25.5813953488372, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17559802, Length=388, Percent_Identity=25.5154639175258, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI72001454, Length=373, Percent_Identity=25.201072386059, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17565138, Length=386, Percent_Identity=24.8704663212435, Blast_Score=95, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17540738, Length=387, Percent_Identity=24.031007751938, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17566914, Length=273, Percent_Identity=27.8388278388278, Blast_Score=89, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6321973, Length=264, Percent_Identity=29.9242424242424, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6325086, Length=90, Percent_Identity=40, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 45765; Mature: 45765

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAPQSAGAAAFAPVRIGPLTLRNRFIKAGANEGMTPEGLPTQALVKHHADLAAGGVGMT
CCCCCCCCCCEECCEEECHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHH
TVAYAAVADDGLTFAHQLSMRAEQVPHLRVLTDAVHREGAAACLQITHAGSFTTMRHGGS
HHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHEEEEECCCCEEEECCCCC
RAPGSASTGLNAFGMMHGVYFQRAMSQADMSRRAAQFAQAARLAREAGFDAVEIHMGHGY
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCH
LLNQFLSPLSNRRRDMFGGSVENRTRFPAMVLRQVKDAVGAEMAVCCKLSVTDGVPGGND
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCC
AADSAITARILEAEGADLLTLSGGRNVESPWALFGSPMPTAQMKAAAPNAMARFGITMLE
HHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHCCCCCCCHHHHHCCCHHHHHHHHHHHH
KRTPRDLTFRELYFLEASRVVRQAVQMPLAYIGGVKSLQNVDALLGEGFDCIALARALIH
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHC
DPALVEHWRNGVAARSACDSCNGCVAKIYDPAGVCCVHGPANDPALTGIPAALKPIQVLK
CHHHHHHHHCCHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCCCCCCCCHHHHHHHHHH
SVKAHTEPSLMGR
HHHHCCCCHHCCC
>Mature Secondary Structure
MEAPQSAGAAAFAPVRIGPLTLRNRFIKAGANEGMTPEGLPTQALVKHHADLAAGGVGMT
CCCCCCCCCCEECCEEECHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHH
TVAYAAVADDGLTFAHQLSMRAEQVPHLRVLTDAVHREGAAACLQITHAGSFTTMRHGGS
HHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHEEEEECCCCEEEECCCCC
RAPGSASTGLNAFGMMHGVYFQRAMSQADMSRRAAQFAQAARLAREAGFDAVEIHMGHGY
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCH
LLNQFLSPLSNRRRDMFGGSVENRTRFPAMVLRQVKDAVGAEMAVCCKLSVTDGVPGGND
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCC
AADSAITARILEAEGADLLTLSGGRNVESPWALFGSPMPTAQMKAAAPNAMARFGITMLE
HHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHCCCCCCCHHHHHCCCHHHHHHHHHHHH
KRTPRDLTFRELYFLEASRVVRQAVQMPLAYIGGVKSLQNVDALLGEGFDCIALARALIH
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHC
DPALVEHWRNGVAARSACDSCNGCVAKIYDPAGVCCVHGPANDPALTGIPAALKPIQVLK
CHHHHHHHHCCHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCCCCCCCCHHHHHHHHHH
SVKAHTEPSLMGR
HHHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]