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The map label for this gene is xylJ [H]

Identifier: 73541201

GI number: 73541201

Start: 1630444

End: 1631226

Strand: Reverse

Name: xylJ [H]

Synonym: Reut_A1510

Alternate gene names: 73541201

Gene position: 1631226-1630444 (Counterclockwise)

Preceding gene: 73541202

Following gene: 73541200

Centisome position: 42.85

GC content: 65.13

Gene sequence:

>783_bases
ATGACAGCCGATCTGATCACCTCGCTCAGCAACGAACTTCACCATGCGCTCGTCACGCGCTTGCCCGTGCCGCCCCTGAG
CGGCCGTGGACTGAGCCTGGGCATCAACGACGCCTACCGCATCCAGCAAGGCTTCATTGCGCACCGGCTCGCGGCCGGCG
ACCGCATCACGGGCAAGAAGATCGGCGTGACCAGCCAGGCCGTGCAGCAAATGCTCGATGTCCACCAGCCGGATTTCGGC
ATCCTGCTGCAGAGCATGCACTATGCCGAAGGCCTGCCGATTCCCACCGACACGCTGATCCAGCCTCGCGCCGAAGGCGA
GATCGCGTTCTTCCTGCGGCGCGACTTGCGCGGTCCCGGCGTCACGCGCGACGATGTGCTCGATGCCACTGAGGCCGTCG
CGGCTTGCTTCGAGATCGTCGATTCGCGCATCCGGGACTGGCGCATCCGCATCGAGGACACCGTTGCCGACAACGCCTCG
TGCGGCGTCTACGTGCTCGGCGACGAGCGTATCAGTCCGCACGCGCTGAACCTGGCTGCTTGCGCCATGACGCTCGAAAA
GAACGGCGAATGCGTGGCCACGGGATCAGGCAGCGCCGCGCTGGGCCACCCCGCCGATGCGGTGGCGTGGCTGGCCAACA
CGCTTGGCAGCTTCGGCATTCCACTACTGGCAGGCGAAACCATTCTATCGGGCTCGCTGGCGGCACTGATTCCCGTGCAA
GCGGGGGACCGGTTGTCCATGCAGATCAGTGGCATCGGTAGCTGCACCGCACAATTCATCTGA

Upstream 100 bases:

>100_bases
ACCGCGACTACGTGGCCTTCAACGCCAAGTATTCCTTCTGACCCGCGTGCGCCGCGGCGGCTTCACGCCGCGGCTGCATC
GACCGAATTCCCCTTCCCCC

Downstream 100 bases:

>100_bases
AGCGCTCACAACGAGGACGCGGCAACGCTCGACGCGGCGTTGCTGCACCGCGAGGATTCCTCCAACTCGACGTTGGTCCA
TTACCCCGCGCGATCCAAAC

Product: 4-oxalocrotonate decarboxylase

Products: 2-oxopent-4-enoate; H2O

Alternate protein names: HPH; 2-oxopent-4-enoate hydratase [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MTADLITSLSNELHHALVTRLPVPPLSGRGLSLGINDAYRIQQGFIAHRLAAGDRITGKKIGVTSQAVQQMLDVHQPDFG
ILLQSMHYAEGLPIPTDTLIQPRAEGEIAFFLRRDLRGPGVTRDDVLDATEAVAACFEIVDSRIRDWRIRIEDTVADNAS
CGVYVLGDERISPHALNLAACAMTLEKNGECVATGSGSAALGHPADAVAWLANTLGSFGIPLLAGETILSGSLAALIPVQ
AGDRLSMQISGIGSCTAQFI

Sequences:

>Translated_260_residues
MTADLITSLSNELHHALVTRLPVPPLSGRGLSLGINDAYRIQQGFIAHRLAAGDRITGKKIGVTSQAVQQMLDVHQPDFG
ILLQSMHYAEGLPIPTDTLIQPRAEGEIAFFLRRDLRGPGVTRDDVLDATEAVAACFEIVDSRIRDWRIRIEDTVADNAS
CGVYVLGDERISPHALNLAACAMTLEKNGECVATGSGSAALGHPADAVAWLANTLGSFGIPLLAGETILSGSLAALIPVQ
AGDRLSMQISGIGSCTAQFI
>Mature_259_residues
TADLITSLSNELHHALVTRLPVPPLSGRGLSLGINDAYRIQQGFIAHRLAAGDRITGKKIGVTSQAVQQMLDVHQPDFGI
LLQSMHYAEGLPIPTDTLIQPRAEGEIAFFLRRDLRGPGVTRDDVLDATEAVAACFEIVDSRIRDWRIRIEDTVADNASC
GVYVLGDERISPHALNLAACAMTLEKNGECVATGSGSAALGHPADAVAWLANTLGSFGIPLLAGETILSGSLAALIPVQA
GDRLSMQISGIGSCTAQFI

Specific function: Conversion of 2-hydroxypent-2,4-dienoate into 4-hydroxy- 2-oxopentanoate [H]

COG id: COG3971

COG function: function code Q; 2-keto-4-pentenoate hydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hydratase/decarboxylase family [H]

Homologues:

Organism=Escherichia coli, GI87081722, Length=262, Percent_Identity=45.0381679389313, Blast_Score=215, Evalue=3e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: 4.2.1.80

Molecular weight: Translated: 27518; Mature: 27387

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTADLITSLSNELHHALVTRLPVPPLSGRGLSLGINDAYRIQQGFIAHRLAAGDRITGKK
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCC
IGVTSQAVQQMLDVHQPDFGILLQSMHYAEGLPIPTDTLIQPRAEGEIAFFLRRDLRGPG
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEHHCCCCC
VTRDDVLDATEAVAACFEIVDSRIRDWRIRIEDTVADNASCGVYVLGDERISPHALNLAA
CCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCEEEEECCCCCCHHHHHHH
CAMTLEKNGECVATGSGSAALGHPADAVAWLANTLGSFGIPLLAGETILSGSLAALIPVQ
HHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCHHCCCHHHCCCEEEEEEEC
AGDRLSMQISGIGSCTAQFI
CCCEEEEEECCCCCCCEECC
>Mature Secondary Structure 
TADLITSLSNELHHALVTRLPVPPLSGRGLSLGINDAYRIQQGFIAHRLAAGDRITGKK
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCC
IGVTSQAVQQMLDVHQPDFGILLQSMHYAEGLPIPTDTLIQPRAEGEIAFFLRRDLRGPG
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEHHCCCCC
VTRDDVLDATEAVAACFEIVDSRIRDWRIRIEDTVADNASCGVYVLGDERISPHALNLAA
CCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCEEEEECCCCCCHHHHHHH
CAMTLEKNGECVATGSGSAALGHPADAVAWLANTLGSFGIPLLAGETILSGSLAALIPVQ
HHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCHHCCCHHHCCCEEEEEEEC
AGDRLSMQISGIGSCTAQFI
CCCEEEEEECCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-hydroxy-2-oxopentanoate

Specific reaction: 4-hydroxy-2-oxopentanoate = 2-oxopent-4-enoate + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1791759 [H]