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The map label for this gene is pntB [H]

Identifier: 73541181

GI number: 73541181

Start: 1609613

End: 1610332

Strand: Direct

Name: pntB [H]

Synonym: Reut_A1489

Alternate gene names: 73541181

Gene position: 1609613-1610332 (Clockwise)

Preceding gene: 73541180

Following gene: 73541184

Centisome position: 42.29

GC content: 58.47

Gene sequence:

>720_bases
GTGAAGTACGCGATCCACCCGGTGGCGGGCCGCATGCCGGGCCATGTAAACGTGCTGCTGGCCGAGGCCGAAGTGCCATA
CGGCCAAGTCTTTGAGATGGAAGACATCAATGGCGAATTTGGCCGGGCCGGCGTGGTGCTGGGCGCCGACTACGTGGTCG
GGTGCAGGCGAGCCGGGATCCGTTCACCTATCAATGACATCGAGGCAATCATGCGCGTGTGCGATATTTTGCAAATCAAG
GCGGGTCCGATCCCTTTCGTCTCGCCAAACACGCTCCTTTCAGAATGTCTCTTCGTGATGGCGGACAATGATCTCGGATC
GTTGGCCGTAGTCGAGCGAGGGACGCTGGTGGGACTGGTGACTTTCCGCGAGATTTTTTCGGCAATGGCGCAACACCATC
GAGGCCGCAATATGGAGCATGGACCACAGGTGCTGGGTATCCGAGCCCGCCACATCATGAACCCAACTCCCATTGTCATC
GACCCGGAAATGGGCATCGCGGAACTGCGAGGACTGATGGTCCAGCACTCCCTGCGATACGTACCGATGGTAAGCAAAGG
CGACTTCCTCGGAATCATCTCGTTCCATGACGTCGCTAAAGCCGTGCACGAGGAGCAGGCGTTCGAGAATCGGATGCTGA
AGTCCTATATCGGCGATTGGCCCGCTGAGGCTATCGCGGTCAGTTTGGCAGCGGAATCCTCTCTTGGCGCCTCGTGTTGA

Upstream 100 bases:

>100_bases
GGAGACCGTGATCACTGTGCCCGGCTACGGGATCGCCGTGGCGCGTGCCTAGCACGCCTTAGAGGAACCGAACGGCCTGC
TCATCGAGGAAGGCGTCACC

Downstream 100 bases:

>100_bases
CGAGTTCGGATTCTGGTGCAAAGGGAGACGGAAATCGAATTGCACCCTGGCAAAAGGCGCGCCAATGCCGAGCGATTCAT
AGCTCGTCGGGTCTTGTACC

Product: CBS

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit beta; Proton-translocating transhydrogenase NADP(H)-binding component; Pyridine nucleotide transhydrogenase subunit beta; dIII [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MKYAIHPVAGRMPGHVNVLLAEAEVPYGQVFEMEDINGEFGRAGVVLGADYVVGCRRAGIRSPINDIEAIMRVCDILQIK
AGPIPFVSPNTLLSECLFVMADNDLGSLAVVERGTLVGLVTFREIFSAMAQHHRGRNMEHGPQVLGIRARHIMNPTPIVI
DPEMGIAELRGLMVQHSLRYVPMVSKGDFLGIISFHDVAKAVHEEQAFENRMLKSYIGDWPAEAIAVSLAAESSLGASC

Sequences:

>Translated_239_residues
MKYAIHPVAGRMPGHVNVLLAEAEVPYGQVFEMEDINGEFGRAGVVLGADYVVGCRRAGIRSPINDIEAIMRVCDILQIK
AGPIPFVSPNTLLSECLFVMADNDLGSLAVVERGTLVGLVTFREIFSAMAQHHRGRNMEHGPQVLGIRARHIMNPTPIVI
DPEMGIAELRGLMVQHSLRYVPMVSKGDFLGIISFHDVAKAVHEEQAFENRMLKSYIGDWPAEAIAVSLAAESSLGASC
>Mature_239_residues
MKYAIHPVAGRMPGHVNVLLAEAEVPYGQVFEMEDINGEFGRAGVVLGADYVVGCRRAGIRSPINDIEAIMRVCDILQIK
AGPIPFVSPNTLLSECLFVMADNDLGSLAVVERGTLVGLVTFREIFSAMAQHHRGRNMEHGPQVLGIRARHIMNPTPIVI
DPEMGIAELRGLMVQHSLRYVPMVSKGDFLGIISFHDVAKAVHEEQAFENRMLKSYIGDWPAEAIAVSLAAESSLGASC

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG0517

COG function: function code R; FOG: CBS domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNT beta subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787886, Length=47, Percent_Identity=59.5744680851064, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012136
- InterPro:   IPR004003 [H]

Pfam domain/function: PF02233 PNTB [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 26017; Mature: 26017

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYAIHPVAGRMPGHVNVLLAEAEVPYGQVFEMEDINGEFGRAGVVLGADYVVGCRRAGI
CCEECCCCCCCCCCCEEEEEEECCCCCCCEEEEECCCCCCCCCEEEECCHHHHHHHHHCC
RSPINDIEAIMRVCDILQIKAGPIPFVSPNTLLSECLFVMADNDLGSLAVVERGTLVGLV
CCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHEEECCCCCCEEEEECCCEEHHH
TFREIFSAMAQHHRGRNMEHGPQVLGIRARHIMNPTPIVIDPEMGIAELRGLMVQHSLRY
HHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHE
VPMVSKGDFLGIISFHDVAKAVHEEQAFENRMLKSYIGDWPAEAIAVSLAAESSLGASC
EEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEHHHHCCCCCCCC
>Mature Secondary Structure
MKYAIHPVAGRMPGHVNVLLAEAEVPYGQVFEMEDINGEFGRAGVVLGADYVVGCRRAGI
CCEECCCCCCCCCCCEEEEEEECCCCCCCEEEEECCCCCCCCCEEEECCHHHHHHHHHCC
RSPINDIEAIMRVCDILQIKAGPIPFVSPNTLLSECLFVMADNDLGSLAVVERGTLVGLV
CCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHEEECCCCCCEEEEECCCEEHHH
TFREIFSAMAQHHRGRNMEHGPQVLGIRARHIMNPTPIVIDPEMGIAELRGLMVQHSLRY
HHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHE
VPMVSKGDFLGIISFHDVAKAVHEEQAFENRMLKSYIGDWPAEAIAVSLAAESSLGASC
EEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHEEHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8075801 [H]