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The map label for this gene is thcD [H]

Identifier: 73541168

GI number: 73541168

Start: 1596040

End: 1597287

Strand: Direct

Name: thcD [H]

Synonym: Reut_A1474

Alternate gene names: 73541168

Gene position: 1596040-1597287 (Clockwise)

Preceding gene: 73541167

Following gene: 73541169

Centisome position: 41.93

GC content: 65.3

Gene sequence:

>1248_bases
ATGATGCCGAGCTCGACGGTCGTCATCGTCGGCGCCGGCCAGGCTGGCTTGCAGGCAGCCACCTCGCTGCGCCAAGCGGG
GTTCGACGGCAGGATTCGGCTGGTCGGCGATGAGCCCTGCATCCCCTACGAGCGCCCACCCCTGTCGAAATCCTACCTTG
CAGGCGAGACCGGGCTCGACGGCCTTTGGTTGCGGCCGGAGATGTTCTACGGAAAGGAGCGAATCGAGCTGGAACTGGGC
CAAACCGCGACCGCGATTGACCGGCAGACGCGGCAAGTCGAACTCGCCTCTGGCCGCAAGGTTGCCTACGACCGATTGGT
GTTAGCGACCGGTGCTCGCTTCCGCCCCTTGTCGGTCCCGGGCGCCGAGCTTGACGGCGTTTTGCCACTGCGCACTCTCG
CCGACGCAGACGCTTTGCGGCCGCGGCTCGCAGAGGCGCGCGAGGTTGTTGTCATCGGCGCCGGCTTCATTGGCCTTGAA
TTTGCCGCCGTCGCTCGCAAGGCAGGGGTTGCCGTACACATCATCGAAATGACGCAGCGGCTGATGGGGCGGGTCGTCTC
CGAGCAGACATCCCGCTTCTACACCAGGGCGCACCGCGACTGGGGCTCAGCGTTTCTGTTCGGCACCGGTGTTGCCCGTA
TTCTCGGCAACAGGCGGGTCAGCGGGGTTGAGACTAGCGATGGCCGCACACTGCCGGCCGACCTCGTATTGATCGGCATC
GGCGTCGTGCCAAATACCGAGATCGCTGCGGCAGCGGGGCTGGCGATCGACAACGGCATCATTGTCGACCAGAACCTGGC
GAGCACCGACCCAACGATCTTCGCGATCGGCGATTGCGCCAACTACCCCAGCCGCTTCGGCCGCTGCCGTCTCGAATCGG
TACAGAACGCCGTCGATCAGGGCCAAGCCGTCGCTGCCGCGATTGTCGGCGAGTCGATACCCTACGACAAGGTGCCATGG
TTCTGGACCGACCAGGCCGATTTGAAACTGCAGATCGCCGGCATCACGGCTGGCCACGACCGTTCGGTATTGCGCGGCGA
TCCTGAGAGCCGCAGCTTCTCGGTGTTCTTCTTCCGCGACGGCACCCTGATCGGCGTCGAGTCGATCAACCGGCCGGCTG
ACCACGTGGTCGCGCGTCGGTTGCTGCTCGCCGACCCGCGTCTGTCCCCGGAACAGGCCGTCGATCCCGACTTCGATCTG
CGGGCGCACGCGATCCGCTTTTCGCGACGTGAGAGGGCGCAAACATGA

Upstream 100 bases:

>100_bases
TTACCGCCTGCGAGCGCTGTCCGACGGAGCGCCTCTCCAGCCGATTGGTGGCGTCGCCGGGGACGGGCGGCGTCGTCGGG
CCCCTGATGGAGACGTGGAT

Downstream 100 bases:

>100_bases
CGGTCAAAACGACGTTGCGGGCGAAACGGGTATTTCCGATCTCGGCTTGCCATCGGGTGGCAGCCAAACAAGCGCGGGGG
CGATGAATATGGCAGGAGAA

Product: FAD-dependent pyridine nucleotide-disulphide oxidoreductase

Products: oxidized ferredoxin; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MMPSSTVVIVGAGQAGLQAATSLRQAGFDGRIRLVGDEPCIPYERPPLSKSYLAGETGLDGLWLRPEMFYGKERIELELG
QTATAIDRQTRQVELASGRKVAYDRLVLATGARFRPLSVPGAELDGVLPLRTLADADALRPRLAEAREVVVIGAGFIGLE
FAAVARKAGVAVHIIEMTQRLMGRVVSEQTSRFYTRAHRDWGSAFLFGTGVARILGNRRVSGVETSDGRTLPADLVLIGI
GVVPNTEIAAAAGLAIDNGIIVDQNLASTDPTIFAIGDCANYPSRFGRCRLESVQNAVDQGQAVAAAIVGESIPYDKVPW
FWTDQADLKLQIAGITAGHDRSVLRGDPESRSFSVFFFRDGTLIGVESINRPADHVVARRLLLADPRLSPEQAVDPDFDL
RAHAIRFSRRERAQT

Sequences:

>Translated_415_residues
MMPSSTVVIVGAGQAGLQAATSLRQAGFDGRIRLVGDEPCIPYERPPLSKSYLAGETGLDGLWLRPEMFYGKERIELELG
QTATAIDRQTRQVELASGRKVAYDRLVLATGARFRPLSVPGAELDGVLPLRTLADADALRPRLAEAREVVVIGAGFIGLE
FAAVARKAGVAVHIIEMTQRLMGRVVSEQTSRFYTRAHRDWGSAFLFGTGVARILGNRRVSGVETSDGRTLPADLVLIGI
GVVPNTEIAAAAGLAIDNGIIVDQNLASTDPTIFAIGDCANYPSRFGRCRLESVQNAVDQGQAVAAAIVGESIPYDKVPW
FWTDQADLKLQIAGITAGHDRSVLRGDPESRSFSVFFFRDGTLIGVESINRPADHVVARRLLLADPRLSPEQAVDPDFDL
RAHAIRFSRRERAQT
>Mature_415_residues
MMPSSTVVIVGAGQAGLQAATSLRQAGFDGRIRLVGDEPCIPYERPPLSKSYLAGETGLDGLWLRPEMFYGKERIELELG
QTATAIDRQTRQVELASGRKVAYDRLVLATGARFRPLSVPGAELDGVLPLRTLADADALRPRLAEAREVVVIGAGFIGLE
FAAVARKAGVAVHIIEMTQRLMGRVVSEQTSRFYTRAHRDWGSAFLFGTGVARILGNRRVSGVETSDGRTLPADLVLIGI
GVVPNTEIAAAAGLAIDNGIIVDQNLASTDPTIFAIGDCANYPSRFGRCRLESVQNAVDQGQAVAAAIVGESIPYDKVPW
FWTDQADLKLQIAGITAGHDRSVLRGDPESRSFSVFFFRDGTLIGVESINRPADHVVARRLLLADPRLSPEQAVDPDFDL
RAHAIRFSRRERAQT

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=373, Percent_Identity=32.7077747989276, Blast_Score=186, Evalue=5e-47,
Organism=Homo sapiens, GI226437568, Length=373, Percent_Identity=32.7077747989276, Blast_Score=185, Evalue=6e-47,
Organism=Homo sapiens, GI21389617, Length=373, Percent_Identity=32.7077747989276, Blast_Score=185, Evalue=6e-47,
Organism=Homo sapiens, GI22202629, Length=338, Percent_Identity=27.2189349112426, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI4757732, Length=338, Percent_Identity=27.2189349112426, Blast_Score=105, Evalue=1e-22,
Organism=Escherichia coli, GI1788892, Length=402, Percent_Identity=36.0696517412935, Blast_Score=201, Evalue=6e-53,
Organism=Escherichia coli, GI1789065, Length=349, Percent_Identity=28.3667621776504, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1789765, Length=295, Percent_Identity=28.8135593220339, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87082354, Length=264, Percent_Identity=26.1363636363636, Blast_Score=69, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17559934, Length=368, Percent_Identity=29.8913043478261, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI32564386, Length=437, Percent_Identity=26.3157894736842, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24639257, Length=381, Percent_Identity=29.9212598425197, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI281359715, Length=381, Percent_Identity=29.9212598425197, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI281359713, Length=381, Percent_Identity=29.9212598425197, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24639250, Length=381, Percent_Identity=29.9212598425197, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI18543267, Length=381, Percent_Identity=29.9212598425197, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24639252, Length=381, Percent_Identity=29.9212598425197, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24585130, Length=406, Percent_Identity=27.5862068965517, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24581020, Length=355, Percent_Identity=25.3521126760563, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI28573993, Length=355, Percent_Identity=25.3521126760563, Blast_Score=112, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3

Molecular weight: Translated: 44912; Mature: 44912

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPSSTVVIVGAGQAGLQAATSLRQAGFDGRIRLVGDEPCIPYERPPLSKSYLAGETGLD
CCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHCCCCCCCC
GLWLRPEMFYGKERIELELGQTATAIDRQTRQVELASGRKVAYDRLVLATGARFRPLSVP
CEEECCHHHCCCEEEEEEECCCHHHHHHHHCEEEECCCCEEHHHEEEEECCCCEEECCCC
GAELDGVLPLRTLADADALRPRLAEAREVVVIGAGFIGLEFAAVARKAGVAVHIIEMTQR
CCCCCCCCCHHHHHCHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHCCCEEEHHHHHHH
LMGRVVSEQTSRFYTRAHRDWGSAFLFGTGVARILGNRRVSGVETSDGRTLPADLVLIGI
HHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCCCCEECCCCCCCCCEEEEEEE
GVVPNTEIAAAAGLAIDNGIIVDQNLASTDPTIFAIGDCANYPSRFGRCRLESVQNAVDQ
CCCCCCCHHHHHCEEECCCEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHC
GQAVAAAIVGESIPYDKVPWFWTDQADLKLQIAGITAGHDRSVLRGDPESRSFSVFFFRD
CHHEEHHHHCCCCCCCCCCEEECCCCCEEEEEEEEECCCCCHHHCCCCCCCEEEEEEEEC
GTLIGVESINRPADHVVARRLLLADPRLSPEQAVDPDFDLRAHAIRFSRRERAQT
CCEEEHHHCCCCHHHHHHHHHHHCCCCCCCHHCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMPSSTVVIVGAGQAGLQAATSLRQAGFDGRIRLVGDEPCIPYERPPLSKSYLAGETGLD
CCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHCCCCCCCC
GLWLRPEMFYGKERIELELGQTATAIDRQTRQVELASGRKVAYDRLVLATGARFRPLSVP
CEEECCHHHCCCEEEEEEECCCHHHHHHHHCEEEECCCCEEHHHEEEEECCCCEEECCCC
GAELDGVLPLRTLADADALRPRLAEAREVVVIGAGFIGLEFAAVARKAGVAVHIIEMTQR
CCCCCCCCCHHHHHCHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHCCCEEEHHHHHHH
LMGRVVSEQTSRFYTRAHRDWGSAFLFGTGVARILGNRRVSGVETSDGRTLPADLVLIGI
HHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCCCCEECCCCCCCCCEEEEEEE
GVVPNTEIAAAAGLAIDNGIIVDQNLASTDPTIFAIGDCANYPSRFGRCRLESVQNAVDQ
CCCCCCCHHHHHCEEECCCEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHC
GQAVAAAIVGESIPYDKVPWFWTDQADLKLQIAGITAGHDRSVLRGDPESRSFSVFFFRD
CHHEEHHHHCCCCCCCCCCEEECCCCCEEEEEEEEECCCCCHHHCCCCCCCEEEEEEEEC
GTLIGVESINRPADHVVARRLLLADPRLSPEQAVDPDFDLRAHAIRFSRRERAQT
CCEEEHHHCCCCHHHHHHHHHHHCCCCCCCHHCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced ferredoxin; NAD+

Specific reaction: reduced ferredoxin + NAD+ = oxidized ferredoxin + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]