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The map label for this gene is rfaD [C]

Identifier: 73541116

GI number: 73541116

Start: 1538563

End: 1539537

Strand: Reverse

Name: rfaD [C]

Synonym: Reut_A1422

Alternate gene names: 73541116

Gene position: 1539537-1538563 (Counterclockwise)

Preceding gene: 73541117

Following gene: 73541112

Centisome position: 40.44

GC content: 67.59

Gene sequence:

>975_bases
ATGAACGTACTGATTACCGGCGGCGCCGGCTTCCTCGGCCTGCAACTGGCGCGCCTGCTGTTGCAGCGCGGCTCCCTGAA
CCTGGATGGCAAGGCGGTCGCCTTCGACCGCCTGACGCTGCTCGACGTCGTTGCGCCGCAAGGCCTCGATGACGCGCGTG
TTCGCGTGGTGACCGGCGACCTGTCGGACCCTGCCGTGCTCGCGCAGGCCATTGACGCCGATACAGGCGCCATCTTCCAT
CTTGCCGCCGTGGTGTCGGGCCAGGCCGAAGCCGACTTTGAGCTCGGCATGCGCGTGAACCTCGACGCTTCGCGCGCGCT
GCTCGAAACGTGCCGCGAGCTCGGCCACAAGCCGCGTGTGTTGTTCACCAGCTCGGTCGCAGTCTATGGCGGCGAGCTGC
CGCCCGTGGTGCAGGACGATACCGCGCTGAACCCGCAATCGTCGTACGGCGTGCAGAAGGCCATCGGCGAATTGCTGCTG
TCCGACTACAGCCGTCGCGGTTTTGTCGACGGGCGCGTACTGCGCCTGCCGACCATCAGCGTGCGGCCCGGCAAGCCCAA
TGCGGCAGCCTCGTCGTTTGCGAGCGGCATCATTCGTGAGCCGCTGTCGGGGGTAGAAGCCAATTGCCCGGTGGCGGCGG
ACACGCCGTTGTGGCTGCTGTCGCCGCGCGCGGCCGTGGCGGCGCTGGTCAACGGCATCGAGCTGGACGGGGAGCGGCTC
GGCAATCGCCGCGTGGTGAATCTTCCGGGGCTGTCCGTCACGGCGGCAGGCATGGTCGAAGCGCTGCGCCGCGTCGCGGG
CGATGCCGTGGCTGACCGTGTCACGTGGCAACGCGAGGAACGCATCGAGAAGATCGTCGGCACGTGGCCGGCGGCGTGGA
ATGCAGAGCGTGCGGTGTCGCTCGGGTTCCAGAGCGATGCGAGCTTTGATGAAGTGATTCGGGCGTATAGGGAGGATGCG
GGGTTGGCGAAGTAA

Upstream 100 bases:

>100_bases
GCTGGATCGGCTGCGAATACCGGCCGCGCGCCGGCACCTCGGCCGGCCTGGGCTGGATCAAGCCGTACCTGAAGCGCTGA
ACCCTCTGAGAAGCATTGCC

Downstream 100 bases:

>100_bases
GCCACAAGGCTTTGCTACTGCCAACGCCTTCGCATCTACCGACGACCAGTCCCCTCTTCCGCGAACGGGAGAGGGGAGCA
AACCAGCGGGATAGTTCTGC

Product: NAD-dependent epimerase/dehydratase:Short-chain dehydrogenase/reductase SDR:3-beta hydroxysteroid dehydrogenase/isomerase:dTDP-4-dehydrorhamnose reductase

Products: ADP-L-glycero-D-manno-heptose [C]

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MNVLITGGAGFLGLQLARLLLQRGSLNLDGKAVAFDRLTLLDVVAPQGLDDARVRVVTGDLSDPAVLAQAIDADTGAIFH
LAAVVSGQAEADFELGMRVNLDASRALLETCRELGHKPRVLFTSSVAVYGGELPPVVQDDTALNPQSSYGVQKAIGELLL
SDYSRRGFVDGRVLRLPTISVRPGKPNAAASSFASGIIREPLSGVEANCPVAADTPLWLLSPRAAVAALVNGIELDGERL
GNRRVVNLPGLSVTAAGMVEALRRVAGDAVADRVTWQREERIEKIVGTWPAAWNAERAVSLGFQSDASFDEVIRAYREDA
GLAK

Sequences:

>Translated_324_residues
MNVLITGGAGFLGLQLARLLLQRGSLNLDGKAVAFDRLTLLDVVAPQGLDDARVRVVTGDLSDPAVLAQAIDADTGAIFH
LAAVVSGQAEADFELGMRVNLDASRALLETCRELGHKPRVLFTSSVAVYGGELPPVVQDDTALNPQSSYGVQKAIGELLL
SDYSRRGFVDGRVLRLPTISVRPGKPNAAASSFASGIIREPLSGVEANCPVAADTPLWLLSPRAAVAALVNGIELDGERL
GNRRVVNLPGLSVTAAGMVEALRRVAGDAVADRVTWQREERIEKIVGTWPAAWNAERAVSLGFQSDASFDEVIRAYREDA
GLAK
>Mature_324_residues
MNVLITGGAGFLGLQLARLLLQRGSLNLDGKAVAFDRLTLLDVVAPQGLDDARVRVVTGDLSDPAVLAQAIDADTGAIFH
LAAVVSGQAEADFELGMRVNLDASRALLETCRELGHKPRVLFTSSVAVYGGELPPVVQDDTALNPQSSYGVQKAIGELLL
SDYSRRGFVDGRVLRLPTISVRPGKPNAAASSFASGIIREPLSGVEANCPVAADTPLWLLSPRAAVAALVNGIELDGERL
GNRRVVNLPGLSVTAAGMVEALRRVAGDAVADRVTWQREERIEKIVGTWPAAWNAERAVSLGFQSDASFDEVIRAYREDA
GLAK

Specific function: Biosynthesis Of The Lipopolysaccharide Precursor ADP-L- Glycerol-D-Mannoheptose. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Caenorhabditis elegans, GI32566934, Length=316, Percent_Identity=24.3670886075949, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24667531, Length=298, Percent_Identity=26.510067114094, Blast_Score=89, Evalue=3e-18,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.20 [C]

Molecular weight: Translated: 34327; Mature: 34327

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLITGGAGFLGLQLARLLLQRGSLNLDGKAVAFDRLTLLDVVAPQGLDDARVRVVTGD
CEEEEECCCHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHCCCCCCCCEEEEEECC
LSDPAVLAQAIDADTGAIFHLAAVVSGQAEADFELGMRVNLDASRALLETCRELGHKPRV
CCCHHHHHHHHCCCCCHHHHHHHHHCCCCCCCEECCEEECCCHHHHHHHHHHHHCCCCCE
LFTSSVAVYGGELPPVVQDDTALNPQSSYGVQKAIGELLLSDYSRRGFVDGRVLRLPTIS
EEECCEEEECCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHCCCCCCCEEEECCEEE
VRPGKPNAAASSFASGIIREPLSGVEANCPVAADTPLWLLSPRAAVAALVNGIELDGERL
ECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCHHHHHHHHHCCCCCHHHC
GNRRVVNLPGLSVTAAGMVEALRRVAGDAVADRVTWQREERIEKIVGTWPAAWNAERAVS
CCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHEE
LGFQSDASFDEVIRAYREDAGLAK
ECCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNVLITGGAGFLGLQLARLLLQRGSLNLDGKAVAFDRLTLLDVVAPQGLDDARVRVVTGD
CEEEEECCCHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHCCCCCCCCEEEEEECC
LSDPAVLAQAIDADTGAIFHLAAVVSGQAEADFELGMRVNLDASRALLETCRELGHKPRV
CCCHHHHHHHHCCCCCHHHHHHHHHCCCCCCCEECCEEECCCHHHHHHHHHHHHCCCCCE
LFTSSVAVYGGELPPVVQDDTALNPQSSYGVQKAIGELLLSDYSRRGFVDGRVLRLPTIS
EEECCEEEECCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHCCCCCCCEEEECCEEE
VRPGKPNAAASSFASGIIREPLSGVEANCPVAADTPLWLLSPRAAVAALVNGIELDGERL
ECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCHHHHHHHHHCCCCCHHHC
GNRRVVNLPGLSVTAAGMVEALRRVAGDAVADRVTWQREERIEKIVGTWPAAWNAERAVS
CCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHEE
LGFQSDASFDEVIRAYREDAGLAK
ECCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.1 {ADP-D-glycero-D-manno-heptose}} [C]

Substrates: ADP-D-glycero-D-manno-heptose [C]

Specific reaction: ADP-D-glycero-D-manno-heptose --> ADP-L-glycero-D-manno-heptose [C]

General reaction: Epimerization [C]

Inhibitor: ADP; ADP glucose; ATP; NADH [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]