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The map label for this gene is acsA1 [H]

Identifier: 73540468

GI number: 73540468

Start: 827173

End: 829188

Strand: Reverse

Name: acsA1 [H]

Synonym: Reut_A0764

Alternate gene names: 73540468

Gene position: 829188-827173 (Counterclockwise)

Preceding gene: 73540469

Following gene: 73540466

Centisome position: 21.78

GC content: 66.32

Gene sequence:

>2016_bases
ATGACCACCCTCGCAGAAGGGCAACTGATGTGGACGCCGTCGGACGCATTCCGCGACGGCAGCCAGCTCGCGCAGTTCAT
GCGCTGGCTCCAGCGCGAGCGGGGGCTCGCCTTCGACGACTACGCCAGCCTCTGGCAATGGTCGGTCACCGAGATCGAGA
GTTTCTGGGATGCGGTGCGCGCCTACTTCGACGTGCACTTCGACACGCCCGCGCGAGGTGTGCTGGACCGGCGCGTGATG
CCGGGAGCGCGTTGGTTCGACGGCGCCAGCCTGAACTACGTACAGCAGGTATTCCGCCATGCCGGCAGCGGCGCCGCCCG
CCAGCGGGCAGCCATCCGCCACGCCGGAGAAAACCGTGCGCTCGAAGAGGTCAGCTGGGATGCGCTGGAAGCGCAGGTCG
CCTCGCTCGCGCATGCGTTGCGCCAGATGGGCGTGGGCCGTGGCGACCGCGTGGCGGGCTACCTGCCGAATATCCCCGCC
ACCATCGTCGCCTTCCTCGCCACCGCCAGCCTCGGCGCGGTGTGGTCCGGCTGCGCGCCGGACATGGGTCAGGTCGCCGT
GATCGACCGCTTTCGCCAGATCGAACCCAAGGTGCTGATCGCCGTCGATGGCTACCGCTACGGCGGCAAGGACTATGACC
GTGCGCCTGTGATAGCGGACCTGGTCGCCGCATTGCCATCTCTGACCGATGTCATCATCGTGCCGCACACCGGCAATCCC
ATGGCTGCGCGCGAGGGTGTCCGTTTGCTCGCATGGCAGGACATGCTGGCCCACCGCGTGCCGCTGGCGATCGAATCCGT
GCCGTTCGACCACCCGTTGTGGATCGTCTATTCGTCGGGCACCACGGGCATGCCCAAGCCGATCGTGCACGGCCATGGCG
GCATCGTCATCGAGCAGCTCAAGCTGATGGCGTTCCACAACAACCTCGGCCCCGACGATGTCTTCCACTGGTACAGCAGC
AGTGGCTGGATCATGTGGAACGCGCAGGTCGCCGGCCTGCTGCTGGGCACGACCATCGCGCTCTATGACGGCAACCCGGC
GTGGCCGGATGCCGGCGTGCTGTGGCGCTTTGTCGACGACGCACGTGTGACGCTGTTTGGCGCAGGCGCGGCGTTCTTCA
CCAACTGCATGAAGGCTGGCGTGGAACCGGCACGCATTGCGGATGTATCCCGGCTGCGCGGGCTCGGCTCAACCGGCTCG
CCGCTGCCGGTGGAAGCGTACGACTGGATCTACCGCCATGTGCGCAGCGATATCTGGCTCGTGCCGATGTCTGGCGGCAC
CGATTTTGCGGGCTCTTTCGTCGCGGGCTGTCCGCTGCTGCCGGTGTATGCCGGGGAGATGCAATGCCGTTGTCTCGGCG
CCAAAGTGGAGGCCTTTGACGACAACGGACAAGCGCTGACCGACAGGGTCGGCGAACTGGTCTGCACGGAGCCGATGCCG
TCGATGCCGCTGTTCCTGTGGGGAGATGCCGACGGCAAGCGCTACCGCGACAGCTACTTCGACACCTATCCCGGTGCCTG
GCGGCACGGCGACTGGATCAAGATCACCGCGCGCGGCGGCGCGATCATCTACGGCCGTTCCGACGCCACCATCAACCGGC
ACGGCATCCGCATGGGCACGAGCGAGCTGTACCGTGTGGTGGAAGACTTGCCTGAAGTCCTCGACAGCATGGTGGTGGAC
CTCGAGTACCTCGGCCGCGAATCGTACATGCCGCTGTTCGTCGTACTGCGCGAGGGAATCGTCCTTGACGATGCGCTGCG
CGACACGCTGCGCATGCGCATCCGCGACGCGCTGTCGTCACGCCACGTGCCCAACGAGATCGTTCAGGTACCGGCCGTGC
CGCGCACACTGTCCGGCAAGAAGATGGAGGTGCCGATCAAGAAGTTGTTGCTCGGGCATGCGCCGGATGGCATCGCCAAC
CGGGACGCCATGGCCAACCCGGCCAGCCTGGACTGGTACTTCGACTACGCCAGGCGCTACCTCGCGGCACGGCAGGCGGA
GTCAGCAACCGCCTGA

Upstream 100 bases:

>100_bases
ATCATGTCAGGCTGAACCCCGGGGCGCATCGAAAGGCACCCGCCCTGCCCCGTTCGACCACACCATCCGATGCCCGCGTA
GACGGGCGCAAGGAGGAGAC

Downstream 100 bases:

>100_bases
CGCTGCCCCATACCCCGGCGAGTATCAGAATTCGACGTCGAGTTCGCTGATCTCGTCGGGCTCTTCGCGCGCTGCCGCCG
TCCACTCCTGCAGTTCGGGC

Product: acetoacetyl-CoA synthetase

Products: NA

Alternate protein names: Acetoacetate--CoA ligase 1; Acyl-activating enzyme 1 [H]

Number of amino acids: Translated: 671; Mature: 670

Protein sequence:

>671_residues
MTTLAEGQLMWTPSDAFRDGSQLAQFMRWLQRERGLAFDDYASLWQWSVTEIESFWDAVRAYFDVHFDTPARGVLDRRVM
PGARWFDGASLNYVQQVFRHAGSGAARQRAAIRHAGENRALEEVSWDALEAQVASLAHALRQMGVGRGDRVAGYLPNIPA
TIVAFLATASLGAVWSGCAPDMGQVAVIDRFRQIEPKVLIAVDGYRYGGKDYDRAPVIADLVAALPSLTDVIIVPHTGNP
MAAREGVRLLAWQDMLAHRVPLAIESVPFDHPLWIVYSSGTTGMPKPIVHGHGGIVIEQLKLMAFHNNLGPDDVFHWYSS
SGWIMWNAQVAGLLLGTTIALYDGNPAWPDAGVLWRFVDDARVTLFGAGAAFFTNCMKAGVEPARIADVSRLRGLGSTGS
PLPVEAYDWIYRHVRSDIWLVPMSGGTDFAGSFVAGCPLLPVYAGEMQCRCLGAKVEAFDDNGQALTDRVGELVCTEPMP
SMPLFLWGDADGKRYRDSYFDTYPGAWRHGDWIKITARGGAIIYGRSDATINRHGIRMGTSELYRVVEDLPEVLDSMVVD
LEYLGRESYMPLFVVLREGIVLDDALRDTLRMRIRDALSSRHVPNEIVQVPAVPRTLSGKKMEVPIKKLLLGHAPDGIAN
RDAMANPASLDWYFDYARRYLAARQAESATA

Sequences:

>Translated_671_residues
MTTLAEGQLMWTPSDAFRDGSQLAQFMRWLQRERGLAFDDYASLWQWSVTEIESFWDAVRAYFDVHFDTPARGVLDRRVM
PGARWFDGASLNYVQQVFRHAGSGAARQRAAIRHAGENRALEEVSWDALEAQVASLAHALRQMGVGRGDRVAGYLPNIPA
TIVAFLATASLGAVWSGCAPDMGQVAVIDRFRQIEPKVLIAVDGYRYGGKDYDRAPVIADLVAALPSLTDVIIVPHTGNP
MAAREGVRLLAWQDMLAHRVPLAIESVPFDHPLWIVYSSGTTGMPKPIVHGHGGIVIEQLKLMAFHNNLGPDDVFHWYSS
SGWIMWNAQVAGLLLGTTIALYDGNPAWPDAGVLWRFVDDARVTLFGAGAAFFTNCMKAGVEPARIADVSRLRGLGSTGS
PLPVEAYDWIYRHVRSDIWLVPMSGGTDFAGSFVAGCPLLPVYAGEMQCRCLGAKVEAFDDNGQALTDRVGELVCTEPMP
SMPLFLWGDADGKRYRDSYFDTYPGAWRHGDWIKITARGGAIIYGRSDATINRHGIRMGTSELYRVVEDLPEVLDSMVVD
LEYLGRESYMPLFVVLREGIVLDDALRDTLRMRIRDALSSRHVPNEIVQVPAVPRTLSGKKMEVPIKKLLLGHAPDGIAN
RDAMANPASLDWYFDYARRYLAARQAESATA
>Mature_670_residues
TTLAEGQLMWTPSDAFRDGSQLAQFMRWLQRERGLAFDDYASLWQWSVTEIESFWDAVRAYFDVHFDTPARGVLDRRVMP
GARWFDGASLNYVQQVFRHAGSGAARQRAAIRHAGENRALEEVSWDALEAQVASLAHALRQMGVGRGDRVAGYLPNIPAT
IVAFLATASLGAVWSGCAPDMGQVAVIDRFRQIEPKVLIAVDGYRYGGKDYDRAPVIADLVAALPSLTDVIIVPHTGNPM
AAREGVRLLAWQDMLAHRVPLAIESVPFDHPLWIVYSSGTTGMPKPIVHGHGGIVIEQLKLMAFHNNLGPDDVFHWYSSS
GWIMWNAQVAGLLLGTTIALYDGNPAWPDAGVLWRFVDDARVTLFGAGAAFFTNCMKAGVEPARIADVSRLRGLGSTGSP
LPVEAYDWIYRHVRSDIWLVPMSGGTDFAGSFVAGCPLLPVYAGEMQCRCLGAKVEAFDDNGQALTDRVGELVCTEPMPS
MPLFLWGDADGKRYRDSYFDTYPGAWRHGDWIKITARGGAIIYGRSDATINRHGIRMGTSELYRVVEDLPEVLDSMVVDL
EYLGRESYMPLFVVLREGIVLDDALRDTLRMRIRDALSSRHVPNEIVQVPAVPRTLSGKKMEVPIKKLLLGHAPDGIANR
DAMANPASLDWYFDYARRYLAARQAESATA

Specific function: Involved in poly-3-hydroxybutyrate degradation. Activates acetoacetate to acetoacetyl-CoA [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI31982927, Length=667, Percent_Identity=37.4812593703148, Blast_Score=468, Evalue=1e-131,
Organism=Homo sapiens, GI28416953, Length=620, Percent_Identity=27.0967741935484, Blast_Score=199, Evalue=5e-51,
Organism=Homo sapiens, GI8923896, Length=662, Percent_Identity=25.8308157099698, Blast_Score=176, Evalue=4e-44,
Organism=Homo sapiens, GI253970489, Length=675, Percent_Identity=25.3333333333333, Blast_Score=172, Evalue=1e-42,
Organism=Homo sapiens, GI13375727, Length=599, Percent_Identity=26.0434056761269, Blast_Score=153, Evalue=5e-37,
Organism=Escherichia coli, GI1790505, Length=627, Percent_Identity=25.0398724082935, Blast_Score=186, Evalue=5e-48,
Organism=Escherichia coli, GI1786529, Length=636, Percent_Identity=26.1006289308176, Blast_Score=153, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17569507, Length=641, Percent_Identity=32.9173166926677, Blast_Score=382, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI71982997, Length=639, Percent_Identity=25.3521126760563, Blast_Score=157, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI71983001, Length=639, Percent_Identity=25.3521126760563, Blast_Score=156, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6323182, Length=634, Percent_Identity=26.9716088328076, Blast_Score=201, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6319264, Length=645, Percent_Identity=25.7364341085271, Blast_Score=195, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24667955, Length=626, Percent_Identity=26.517571884984, Blast_Score=194, Evalue=2e-49,
Organism=Drosophila melanogaster, GI62472339, Length=530, Percent_Identity=27.1698113207547, Blast_Score=178, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24667959, Length=499, Percent_Identity=26.8537074148297, Blast_Score=157, Evalue=3e-38,
Organism=Drosophila melanogaster, GI21355225, Length=609, Percent_Identity=25.615763546798, Blast_Score=154, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005914
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.16 [H]

Molecular weight: Translated: 74173; Mature: 74042

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLAEGQLMWTPSDAFRDGSQLAQFMRWLQRERGLAFDDYASLWQWSVTEIESFWDAVR
CCCCCCCEEEECCCHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
AYFDVHFDTPARGVLDRRVMPGARWFDGASLNYVQQVFRHAGSGAARQRAAIRHAGENRA
HHHEEECCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCH
LEEVSWDALEAQVASLAHALRQMGVGRGDRVAGYLPNIPATIVAFLATASLGAVWSGCAP
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
DMGQVAVIDRFRQIEPKVLIAVDGYRYGGKDYDRAPVIADLVAALPSLTDVIIVPHTGNP
CCCCHHHHHHHHHCCCEEEEEECCEECCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCC
MAAREGVRLLAWQDMLAHRVPLAIESVPFDHPLWIVYSSGTTGMPKPIVHGHGGIVIEQL
HHHHCCCCHHHHHHHHHHHCCEEEECCCCCCCEEEEECCCCCCCCCCEEECCCCCHHHHH
KLMAFHNNLGPDDVFHWYSSSGWIMWNAQVAGLLLGTTIALYDGNPAWPDAGVLWRFVDD
HHHHHHCCCCHHHHHHHCCCCCEEEECHHHHHEEEEEEEEEECCCCCCCCCCCEEEEECC
ARVTLFGAGAAFFTNCMKAGVEPARIADVSRLRGLGSTGSPLPVEAYDWIYRHVRSDIWL
CEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCEEE
VPMSGGTDFAGSFVAGCPLLPVYAGEMQCRCLGAKVEAFDDNGQALTDRVGELVCTEPMP
EECCCCCCCCCHHHHCCCCCEEECCCEEEEEECCEEEEECCCCHHHHHHHCCEEECCCCC
SMPLFLWGDADGKRYRDSYFDTYPGAWRHGDWIKITARGGAIIYGRSDATINRHGIRMGT
CCCEEEEECCCCCHHHHHHCCCCCCCCCCCCEEEEEECCCEEEECCCCCEEECCCCCCCH
SELYRVVEDLPEVLDSMVVDLEYLGRESYMPLFVVLREGIVLDDALRDTLRMRIRDALSS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCEEHHHHHHHHHHHHHHHHHH
RHVPNEIVQVPAVPRTLSGKKMEVPIKKLLLGHAPDGIANRDAMANPASLDWYFDYARRY
CCCCHHHHCCCCCCCCCCCCEEECCHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
LAARQAESATA
HHHHHHCCCCC
>Mature Secondary Structure 
TTLAEGQLMWTPSDAFRDGSQLAQFMRWLQRERGLAFDDYASLWQWSVTEIESFWDAVR
CCCCCCEEEECCCHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
AYFDVHFDTPARGVLDRRVMPGARWFDGASLNYVQQVFRHAGSGAARQRAAIRHAGENRA
HHHEEECCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCH
LEEVSWDALEAQVASLAHALRQMGVGRGDRVAGYLPNIPATIVAFLATASLGAVWSGCAP
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
DMGQVAVIDRFRQIEPKVLIAVDGYRYGGKDYDRAPVIADLVAALPSLTDVIIVPHTGNP
CCCCHHHHHHHHHCCCEEEEEECCEECCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCC
MAAREGVRLLAWQDMLAHRVPLAIESVPFDHPLWIVYSSGTTGMPKPIVHGHGGIVIEQL
HHHHCCCCHHHHHHHHHHHCCEEEECCCCCCCEEEEECCCCCCCCCCEEECCCCCHHHHH
KLMAFHNNLGPDDVFHWYSSSGWIMWNAQVAGLLLGTTIALYDGNPAWPDAGVLWRFVDD
HHHHHHCCCCHHHHHHHCCCCCEEEECHHHHHEEEEEEEEEECCCCCCCCCCCEEEEECC
ARVTLFGAGAAFFTNCMKAGVEPARIADVSRLRGLGSTGSPLPVEAYDWIYRHVRSDIWL
CEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCEEE
VPMSGGTDFAGSFVAGCPLLPVYAGEMQCRCLGAKVEAFDDNGQALTDRVGELVCTEPMP
EECCCCCCCCCHHHHCCCCCEEECCCEEEEEECCEEEEECCCCHHHHHHHCCEEECCCCC
SMPLFLWGDADGKRYRDSYFDTYPGAWRHGDWIKITARGGAIIYGRSDATINRHGIRMGT
CCCEEEEECCCCCHHHHHHCCCCCCCCCCCCEEEEEECCCEEEECCCCCEEECCCCCCCH
SELYRVVEDLPEVLDSMVVDLEYLGRESYMPLFVVLREGIVLDDALRDTLRMRIRDALSS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCEEHHHHHHHHHHHHHHHHHH
RHVPNEIVQVPAVPRTLSGKKMEVPIKKLLLGHAPDGIANRDAMANPASLDWYFDYARRY
CCCCHHHHCCCCCCCCCCCCEEECCHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
LAARQAESATA
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10735852; 11481430 [H]