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The map label for this gene is rfbD [H]

Identifier: 73540425

GI number: 73540425

Start: 780808

End: 781776

Strand: Direct

Name: rfbD [H]

Synonym: Reut_A0719

Alternate gene names: 73540425

Gene position: 780808-781776 (Clockwise)

Preceding gene: 73540424

Following gene: 73540426

Centisome position: 20.51

GC content: 59.13

Gene sequence:

>969_bases
ATGAAGATTCTGATTCTGGGAGCAACTGGGTTTGTCGGCAGTTATCTCATGGAGCGCGCCGTCGCGGCCGGCCACGAGGT
AACAGGGACCCATTACCACGCCACCATCGATACACGGGACTGGGTAGGCTTTTCTGACCAGCTGATCCGGTGCGACATTC
GCTATCGTGAACAGATCGATGCCGCCATCGTGCGTTCGCGCCCCGACGTGATCTACCTCCTGTCCGCCCAGAGCTACCCG
GCATTGTCCTGGACTGCGCCTGCGGAAACTCTGGAGACCAATGTGGTTGGCACCTCCAACGTCTTCGAATCGATCAAGGC
CGCCGGGATTGACCCGGTGGTTGTTGTGGCATGCTCCAGCGCGCAATACGGCGAAGTGCAGGCAGACGCGATTCCGATCA
AGGAATCCCATCCGCTGCGTCCGATGCATCCGTACGGTGTCAGCAAGGTTGCCACCGAGATGCTTGCCATGCAGTACTGG
GTTAACAATGGCATCCGTAGCGTCTGCGCACGCATCTTCAATACCACTGGCCCACGCAAGACCGGCGATGTCTGCGCCGA
CTTCACGTATCGTGTCGCGCGCATCGAGCAAGGCCTCATTCCGCCTGTGCTGCGCGTAGGCAATCTCGAGACCCACCGCG
CAATCACCGATGTGCGAGATCTGGCCCGAGCACTGGAGCTACTGGCCGAGCGTGGCGCGCCGGGTGGTGTCTACAACGTG
AGCGGTTCGCGTGCGTACCAGATCCGCGAGATTGTCGACCTGATTCAGGCGGCATCGACCATCAGCTTCACACTTGAAAC
GGACCAGGCCCTGCTGCGGCCCAGCGACGAAAAAGTAATCTTTGGCGATTCGAGCAGCTTGGTCGAGGCAACGGGCTGGG
CTCAAGAAGTAACTATCGCGAAGACGATAGGCGACATGCTCGCCTATTGGCGAAACATGCTTGGTATGCAAAAGAAGGAG
GTCGCATGA

Upstream 100 bases:

>100_bases
CTGTGGCGAGTTCTGGCGGAAGATGTGGACCCCACGCCGTACGCGAGTGTCAAGGACCGACTGCCACAGAACGACCATCA
AATGAATAAGGCCTGAAGCT

Downstream 100 bases:

>100_bases
TCATCCGATCTCGAGCGCCTTTGCGTCTTGGCCTGGCCGGCGGCGGCACGGACGTATCGCCGTATTCCGACCGCTTCGGT
GGTCACGTGCTCAACGTGAC

Product: NAD-dependent epimerase/dehydratase:3-beta hydroxysteroid dehydrogenase/isomerase:dTDP-4-dehydrorhamnose reductase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MKILILGATGFVGSYLMERAVAAGHEVTGTHYHATIDTRDWVGFSDQLIRCDIRYREQIDAAIVRSRPDVIYLLSAQSYP
ALSWTAPAETLETNVVGTSNVFESIKAAGIDPVVVVACSSAQYGEVQADAIPIKESHPLRPMHPYGVSKVATEMLAMQYW
VNNGIRSVCARIFNTTGPRKTGDVCADFTYRVARIEQGLIPPVLRVGNLETHRAITDVRDLARALELLAERGAPGGVYNV
SGSRAYQIREIVDLIQAASTISFTLETDQALLRPSDEKVIFGDSSSLVEATGWAQEVTIAKTIGDMLAYWRNMLGMQKKE
VA

Sequences:

>Translated_322_residues
MKILILGATGFVGSYLMERAVAAGHEVTGTHYHATIDTRDWVGFSDQLIRCDIRYREQIDAAIVRSRPDVIYLLSAQSYP
ALSWTAPAETLETNVVGTSNVFESIKAAGIDPVVVVACSSAQYGEVQADAIPIKESHPLRPMHPYGVSKVATEMLAMQYW
VNNGIRSVCARIFNTTGPRKTGDVCADFTYRVARIEQGLIPPVLRVGNLETHRAITDVRDLARALELLAERGAPGGVYNV
SGSRAYQIREIVDLIQAASTISFTLETDQALLRPSDEKVIFGDSSSLVEATGWAQEVTIAKTIGDMLAYWRNMLGMQKKE
VA
>Mature_322_residues
MKILILGATGFVGSYLMERAVAAGHEVTGTHYHATIDTRDWVGFSDQLIRCDIRYREQIDAAIVRSRPDVIYLLSAQSYP
ALSWTAPAETLETNVVGTSNVFESIKAAGIDPVVVVACSSAQYGEVQADAIPIKESHPLRPMHPYGVSKVATEMLAMQYW
VNNGIRSVCARIFNTTGPRKTGDVCADFTYRVARIEQGLIPPVLRVGNLETHRAITDVRDLARALELLAERGAPGGVYNV
SGSRAYQIREIVDLIQAASTISFTLETDQALLRPSDEKVIFGDSSSLVEATGWAQEVTIAKTIGDMLAYWRNMLGMQKKE
VA

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=333, Percent_Identity=28.5285285285285, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI4504031, Length=337, Percent_Identity=24.0356083086053, Blast_Score=83, Evalue=4e-16,
Organism=Escherichia coli, GI1788366, Length=348, Percent_Identity=26.1494252873563, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI48994969, Length=269, Percent_Identity=25.6505576208178, Blast_Score=68, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17539532, Length=331, Percent_Identity=25.3776435045317, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17539422, Length=341, Percent_Identity=24.3401759530792, Blast_Score=78, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI133901786, Length=341, Percent_Identity=24.3401759530792, Blast_Score=78, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI133901788, Length=341, Percent_Identity=24.3401759530792, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI133901790, Length=341, Percent_Identity=24.3401759530792, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17539424, Length=341, Percent_Identity=24.3401759530792, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17507723, Length=349, Percent_Identity=24.0687679083095, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24158427, Length=338, Percent_Identity=24.2603550295858, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI21356223, Length=329, Percent_Identity=26.7477203647416, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 35265; Mature: 35265

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILILGATGFVGSYLMERAVAAGHEVTGTHYHATIDTRDWVGFSDQLIRCDIRYREQID
CEEEEEECCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCEEEEEHHHHHHHH
AAIVRSRPDVIYLLSAQSYPALSWTAPAETLETNVVGTSNVFESIKAAGIDPVVVVACSS
HHHHCCCCCEEEEEECCCCCCEEECCCHHHHHCCCCCCHHHHHHHHHCCCCEEEEEEECC
AQYGEVQADAIPIKESHPLRPMHPYGVSKVATEMLAMQYWVNNGIRSVCARIFNTTGPRK
CCCCCEECCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TGDVCADFTYRVARIEQGLIPPVLRVGNLETHRAITDVRDLARALELLAERGAPGGVYNV
CCCHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEC
SGSRAYQIREIVDLIQAASTISFTLETDQALLRPSDEKVIFGDSSSLVEATGWAQEVTIA
CCCCHHHHHHHHHHHHHHHHEEEEEECCHHHHCCCCCEEEECCCCCHHHHCCCHHHHHHH
KTIGDMLAYWRNMLGMQKKEVA
HHHHHHHHHHHHHHCCHHHHCC
>Mature Secondary Structure
MKILILGATGFVGSYLMERAVAAGHEVTGTHYHATIDTRDWVGFSDQLIRCDIRYREQID
CEEEEEECCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCEEEEEHHHHHHHH
AAIVRSRPDVIYLLSAQSYPALSWTAPAETLETNVVGTSNVFESIKAAGIDPVVVVACSS
HHHHCCCCCEEEEEECCCCCCEEECCCHHHHHCCCCCCHHHHHHHHHCCCCEEEEEEECC
AQYGEVQADAIPIKESHPLRPMHPYGVSKVATEMLAMQYWVNNGIRSVCARIFNTTGPRK
CCCCCEECCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TGDVCADFTYRVARIEQGLIPPVLRVGNLETHRAITDVRDLARALELLAERGAPGGVYNV
CCCHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEC
SGSRAYQIREIVDLIQAASTISFTLETDQALLRPSDEKVIFGDSSSLVEATGWAQEVTIA
CCCCHHHHHHHHHHHHHHHHEEEEEECCHHHHCCCCCEEEECCCCCHHHHCCCHHHHHHH
KTIGDMLAYWRNMLGMQKKEVA
HHHHHHHHHHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1372980; 7540582; 10952301 [H]