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The map label for this gene is fdoI [H]

Identifier: 73540391

GI number: 73540391

Start: 738548

End: 739744

Strand: Direct

Name: fdoI [H]

Synonym: Reut_A0685

Alternate gene names: 73540391

Gene position: 738548-739744 (Clockwise)

Preceding gene: 73540390

Following gene: 73540392

Centisome position: 19.4

GC content: 66.83

Gene sequence:

>1197_bases
ATGAGGCCCACGCAGAACCCTTGCCGCTGGCTCGCTGCCAGCGCACTGGCCTGGCTGGCACTCGCGGGCAGCGCCGCGCT
GGCGCAGGCACCGGCTTCGGCGCCGCCGGCTTCTGCCGCCGCTATCGCCGCACAACCGGCGGACGCGAACGACCCGGCCA
CGCATCCTGCGCAGCCGCTGGCGGGTATAGCCTCGGAGAACATCTTCAACGTGCCTCGCCGCGATATCGCCGGCGAGGCC
AGGTCGCAGCAGGAACGCACGGTGACGCAGCCGGGCAACAACGCGCCGGTATGGCGCGAGGTCAATTCCGACCAGGCTCA
CTACAGCAGCCTGCCGGCCAAGGAAGCCGGCGTGCTGATCCAGCGGACCGGCCAGAAGTGGCGGCTGTTCCGCAATGGCG
TGATCACCGTATGGGGCGGCTGGCTGCTGGTGATCGTGCCGCTCGCGATCCTCGGTTTCTTCGCCTGGCGCGGGACCATC
CCGCTGCGCGAGCCGCGCACCGGGCGCATGATCGAGCGCTTCACGCCGATCGAGCGCATCGTGCACTGGACCATGGCGAT
CTCATTCGTACTGCTCGGCGTGTCGGGCATCGTGATGCTGTTCGGCAAGCATTTCCTGCTGCCGATCATGGGACACACGC
TGTTCGGCTGGCTGAGCTACCTGCTGAAGAACATCCACAACCTGGCGGGGCCAGTGTTCACGTTGTCGATCATCGTCGCC
TTCGTGATTTTCGTGCGCGACAACCTGCCCAGCCGCGACGACGTCCGTTGGGTGACTAGCCTCGGCGGGCTGGCCAGCGG
CAAGCATGTGCCGAGCGGCCGGTTCAATTTCGGCGAGAAGATGTGGTTCTGGGGCGGGCTGGTGATCTTCGGTCTGATCC
TGTCCGCGTCGGGCTGGGTGCTCGACATGATCGTGCCGGGGCTCGACTACTACCGCGCCACCATGCAGATCGCCAACGTG
ATCCATGGCATCGCGGCCGTGCTGATGATCGCGATGTCGCTGGGCCATATCTACATGGGCACCATCGGCATGGAAGGCGC
CTACCGTGCGATGCGCGACGGCTGGGTCGACGAAGCCTGGGCCAGGGAGCACCACGAACTCTGGTACGACGACATCAAGT
CCGGCAAGATCCCGGCGCAGCGCTCGACGACGCCGGAACCAGGCACCGCTGGCCGGCGTACCCCGGGCGAGGCCTGA

Upstream 100 bases:

>100_bases
TGGCAGAACCAGATCCGCCAGCGCAATCAGCTGCAGAACGAATACAACCGTACGCCGTGACTGCGCAGCAGGCAGGGCGG
GGCGATCCGAAGGAGCGCGT

Downstream 100 bases:

>100_bases
GACATTGCTGCAGACCCGCCTTCACGGATCACGACCATGAAACGCATACTGATGCTGATTGCCCTGGCCGCAGGCACCGC
TGGTCTCGCCGGCTCGCAAG

Product: formate dehydrogenase gamma subunit

Products: CO2; NADH

Alternate protein names: Aerobic formate dehydrogenase cytochrome b556 subunit; FDH-Z subunit gamma; Formate dehydrogenase-O subunit gamma [H]

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MRPTQNPCRWLAASALAWLALAGSAALAQAPASAPPASAAAIAAQPADANDPATHPAQPLAGIASENIFNVPRRDIAGEA
RSQQERTVTQPGNNAPVWREVNSDQAHYSSLPAKEAGVLIQRTGQKWRLFRNGVITVWGGWLLVIVPLAILGFFAWRGTI
PLREPRTGRMIERFTPIERIVHWTMAISFVLLGVSGIVMLFGKHFLLPIMGHTLFGWLSYLLKNIHNLAGPVFTLSIIVA
FVIFVRDNLPSRDDVRWVTSLGGLASGKHVPSGRFNFGEKMWFWGGLVIFGLILSASGWVLDMIVPGLDYYRATMQIANV
IHGIAAVLMIAMSLGHIYMGTIGMEGAYRAMRDGWVDEAWAREHHELWYDDIKSGKIPAQRSTTPEPGTAGRRTPGEA

Sequences:

>Translated_398_residues
MRPTQNPCRWLAASALAWLALAGSAALAQAPASAPPASAAAIAAQPADANDPATHPAQPLAGIASENIFNVPRRDIAGEA
RSQQERTVTQPGNNAPVWREVNSDQAHYSSLPAKEAGVLIQRTGQKWRLFRNGVITVWGGWLLVIVPLAILGFFAWRGTI
PLREPRTGRMIERFTPIERIVHWTMAISFVLLGVSGIVMLFGKHFLLPIMGHTLFGWLSYLLKNIHNLAGPVFTLSIIVA
FVIFVRDNLPSRDDVRWVTSLGGLASGKHVPSGRFNFGEKMWFWGGLVIFGLILSASGWVLDMIVPGLDYYRATMQIANV
IHGIAAVLMIAMSLGHIYMGTIGMEGAYRAMRDGWVDEAWAREHHELWYDDIKSGKIPAQRSTTPEPGTAGRRTPGEA
>Mature_398_residues
MRPTQNPCRWLAASALAWLALAGSAALAQAPASAPPASAAAIAAQPADANDPATHPAQPLAGIASENIFNVPRRDIAGEA
RSQQERTVTQPGNNAPVWREVNSDQAHYSSLPAKEAGVLIQRTGQKWRLFRNGVITVWGGWLLVIVPLAILGFFAWRGTI
PLREPRTGRMIERFTPIERIVHWTMAISFVLLGVSGIVMLFGKHFLLPIMGHTLFGWLSYLLKNIHNLAGPVFTLSIIVA
FVIFVRDNLPSRDDVRWVTSLGGLASGKHVPSGRFNFGEKMWFWGGLVIFGLILSASGWVLDMIVPGLDYYRATMQIANV
IHGIAAVLMIAMSLGHIYMGTIGMEGAYRAMRDGWVDEAWAREHHELWYDDIKSGKIPAQRSTTPEPGTAGRRTPGEA

Specific function: Allows to use formate as major electron donor during aerobic respiration. Subunit gamma is probably the cytochrome b556(FDO) component of the formate dehydrogenase [H]

COG id: COG2864

COG function: function code C; Cytochrome b subunit of formate dehydrogenase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the formate dehydrogenase gamma subunit family [H]

Homologues:

Organism=Escherichia coli, GI1790325, Length=206, Percent_Identity=33.495145631068, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1787750, Length=227, Percent_Identity=28.1938325991189, Blast_Score=79, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005797
- InterPro:   IPR016174
- InterPro:   IPR006471 [H]

Pfam domain/function: PF00033 Cytochrom_B_N [H]

EC number: 1.2.1.2

Molecular weight: Translated: 43567; Mature: 43567

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPTQNPCRWLAASALAWLALAGSAALAQAPASAPPASAAAIAAQPADANDPATHPAQPL
CCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHEECCCCCCCCCCCCCCHH
AGIASENIFNVPRRDIAGEARSQQERTVTQPGNNAPVWREVNSDQAHYSSLPAKEAGVLI
HHHHCCCHHCCCCHHHCCHHHHHHHHHCCCCCCCCCCEECCCCCHHHHHCCCCHHHCCHH
QRTGQKWRLFRNGVITVWGGWLLVIVPLAILGFFAWRGTIPLREPRTGRMIERFTPIERI
HHCCHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
VHWTMAISFVLLGVSGIVMLFGKHFLLPIMGHTLFGWLSYLLKNIHNLAGPVFTLSIIVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FVIFVRDNLPSRDDVRWVTSLGGLASGKHVPSGRFNFGEKMWFWGGLVIFGLILSASGWV
HHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
LDMIVPGLDYYRATMQIANVIHGIAAVLMIAMSLGHIYMGTIGMEGAYRAMRDGWVDEAW
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHH
AREHHELWYDDIKSGKIPAQRSTTPEPGTAGRRTPGEA
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRPTQNPCRWLAASALAWLALAGSAALAQAPASAPPASAAAIAAQPADANDPATHPAQPL
CCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHEECCCCCCCCCCCCCCHH
AGIASENIFNVPRRDIAGEARSQQERTVTQPGNNAPVWREVNSDQAHYSSLPAKEAGVLI
HHHHCCCHHCCCCHHHCCHHHHHHHHHCCCCCCCCCCEECCCCCHHHHHCCCCHHHCCHH
QRTGQKWRLFRNGVITVWGGWLLVIVPLAILGFFAWRGTIPLREPRTGRMIERFTPIERI
HHCCHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
VHWTMAISFVLLGVSGIVMLFGKHFLLPIMGHTLFGWLSYLLKNIHNLAGPVFTLSIIVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FVIFVRDNLPSRDDVRWVTSLGGLASGKHVPSGRFNFGEKMWFWGGLVIFGLILSASGWV
HHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
LDMIVPGLDYYRATMQIANVIHGIAAVLMIAMSLGHIYMGTIGMEGAYRAMRDGWVDEAW
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHH
AREHHELWYDDIKSGKIPAQRSTTPEPGTAGRRTPGEA
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]