Click here to switch to the map view.

The map label for this gene is fdhB1 [H]

Identifier: 73540389

GI number: 73540389

Start: 737561

End: 738274

Strand: Direct

Name: fdhB1 [H]

Synonym: Reut_A0683

Alternate gene names: 73540389

Gene position: 737561-738274 (Clockwise)

Preceding gene: 73540388

Following gene: 73540390

Centisome position: 19.38

GC content: 65.97

Gene sequence:

>714_bases
ATGGCCCGCATGAAATTTATCTGTGACGCCGAGCGCTGCATCGAATGCAACAGCTGCGTCACCGCCTGCAAGAACGAGCA
TGAGGTGCCATGGGGCGTGAACCGGCGCCGCGTGGTCACTGTCAACGACGGCGTGGTCGGCGCCGAGAAATCGATCTCGG
TCGCCTGCATGCACTGTTCGGACGCGCCGTGCATGGCGGTCTGCCCGGTCGACTGCTTCTACCGCACCGAAGACGGCGTG
GTGCTGCACGACAAGGATGTGTGCATCGGCTGCGGCTACTGCTCGTACGCATGCCCGTTCGGTGCGCCGCAGTTCCCGTC
CACGGGCACCTTCGGCGTGCGCGGCAAGATGGATAAATGCACATTCTGCGCGGGTGGGCCCGAGAAGAACGGCTCGGAGG
CCGAGTTCGAGAAGTACGGCCGCAACCGCCTGGCCGAAGGCAAGCTGCCGCTGTGCGCGGAAATGTGCTCGACCAAGGCG
CTGCTCGGCGGCGATGGCGACGTGATTGCCGACATCCTGCGCAATCGAGTGATCAAGCGCGGCAAAGGGGGCGATGTGTT
CGGCTGGGGTACCGCATACGGCACGAAGGGCGGTGCGCAACAGGCGCCGGCCGCGCCCGCTGCACCTGCCGCTCCCGCAC
CGGGTAATGCGAGCGGAAGCGCGAACGGGAGCGCAACCGGGAGCGCAACCGCGAATGGGGGGCAACCATCATGA

Upstream 100 bases:

>100_bases
ACGGCTATGACTCGGTGACGATGATGCAGGAAACCAAGACCACGATCTGCCAGATCGAACGCTTTGCCTGAATGCATGAC
ATTGATCGCAGGATGACACC

Downstream 100 bases:

>100_bases
CGCGCATCGCAATGATGGCCGTGCTGGCCGCGGCCGGCGTGCTGCTCGCAGCCTGTGGCGAAAAGGCACAGACCACGACC
GCCTCGCACAAGAAGACCGA

Product: 4Fe-4S ferredoxin, iron-sulfur binding

Products: CO2; NADH

Alternate protein names: NA

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MARMKFICDAERCIECNSCVTACKNEHEVPWGVNRRRVVTVNDGVVGAEKSISVACMHCSDAPCMAVCPVDCFYRTEDGV
VLHDKDVCIGCGYCSYACPFGAPQFPSTGTFGVRGKMDKCTFCAGGPEKNGSEAEFEKYGRNRLAEGKLPLCAEMCSTKA
LLGGDGDVIADILRNRVIKRGKGGDVFGWGTAYGTKGGAQQAPAAPAAPAAPAPGNASGSANGSATGSATANGGQPS

Sequences:

>Translated_237_residues
MARMKFICDAERCIECNSCVTACKNEHEVPWGVNRRRVVTVNDGVVGAEKSISVACMHCSDAPCMAVCPVDCFYRTEDGV
VLHDKDVCIGCGYCSYACPFGAPQFPSTGTFGVRGKMDKCTFCAGGPEKNGSEAEFEKYGRNRLAEGKLPLCAEMCSTKA
LLGGDGDVIADILRNRVIKRGKGGDVFGWGTAYGTKGGAQQAPAAPAAPAAPAPGNASGSANGSATGSATANGGQPS
>Mature_236_residues
ARMKFICDAERCIECNSCVTACKNEHEVPWGVNRRRVVTVNDGVVGAEKSISVACMHCSDAPCMAVCPVDCFYRTEDGVV
LHDKDVCIGCGYCSYACPFGAPQFPSTGTFGVRGKMDKCTFCAGGPEKNGSEAEFEKYGRNRLAEGKLPLCAEMCSTKAL
LGGDGDVIADILRNRVIKRGKGGDVFGWGTAYGTKGGAQQAPAAPAAPAAPAPGNASGSANGSATGSATANGGQPS

Specific function: This chain is an electron transfer unit containing 18 cysteine residues, 16 of which occur in four clusters [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787122, Length=220, Percent_Identity=33.6363636363636, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787872, Length=169, Percent_Identity=35.5029585798817, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI226510944, Length=171, Percent_Identity=35.0877192982456, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1789370, Length=202, Percent_Identity=29.2079207920792, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI2367345, Length=211, Percent_Identity=30.8056872037915, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1789079, Length=232, Percent_Identity=28.448275862069, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI87082114, Length=181, Percent_Identity=28.1767955801105, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI87082180, Length=237, Percent_Identity=28.2700421940928, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI2367245, Length=157, Percent_Identity=33.1210191082803, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1790326, Length=192, Percent_Identity=25.5208333333333, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI1788811, Length=125, Percent_Identity=32, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR000813 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: 1.2.1.2

Molecular weight: Translated: 24494; Mature: 24363

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
10.1 %Cys+Met (Translated Protein)
7.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
9.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARMKFICDAERCIECNSCVTACKNEHEVPWGVNRRRVVTVNDGVVGAEKSISVACMHCS
CCCCCEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCEECCCCCEEEEEEECC
DAPCMAVCPVDCFYRTEDGVVLHDKDVCIGCGYCSYACPFGAPQFPSTGTFGVRGKMDKC
CCCEEEECCCEEEEECCCCEEEECCCEEEECCHHHCCCCCCCCCCCCCCCCCCCCCCCCE
TFCAGGPEKNGSEAEFEKYGRNRLAEGKLPLCAEMCSTKALLGGDGDVIADILRNRVIKR
EEECCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHCCCEEECCCHHHHHHHHHHHHHHC
GKGGDVFGWGTAYGTKGGAQQAPAAPAAPAAPAPGNASGSANGSATGSATANGGQPS
CCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ARMKFICDAERCIECNSCVTACKNEHEVPWGVNRRRVVTVNDGVVGAEKSISVACMHCS
CCCCEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCEECCCCCEEEEEEECC
DAPCMAVCPVDCFYRTEDGVVLHDKDVCIGCGYCSYACPFGAPQFPSTGTFGVRGKMDKC
CCCEEEECCCEEEEECCCCEEEECCCEEEECCHHHCCCCCCCCCCCCCCCCCCCCCCCCE
TFCAGGPEKNGSEAEFEKYGRNRLAEGKLPLCAEMCSTKALLGGDGDVIADILRNRVIKR
EEECCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHCCCEEECCCHHHHHHHHHHHHHHC
GKGGDVFGWGTAYGTKGGAQQAPAAPAAPAAPAPGNASGSANGSATGSATANGGQPS
CCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1781728; 14500908 [H]