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The map label for this gene is metG [H]

Identifier: 73540380

GI number: 73540380

Start: 725582

End: 727648

Strand: Reverse

Name: metG [H]

Synonym: Reut_A0674

Alternate gene names: 73540380

Gene position: 727648-725582 (Counterclockwise)

Preceding gene: 73540381

Following gene: 73540376

Centisome position: 19.12

GC content: 63.13

Gene sequence:

>2067_bases
ATGACCGCACGTCGTATCCTCGTCACATCTGCCCTGCCATATGCCAACGGCCAGATTCATATCGGCCACCTGGTGGAGTA
CATCCAGACCGACATCTGGGTGCGCTTCCAGCGCATGATGGGCAACGAGGTTTACTACGTCGGCGCCGACGACACCCACG
GTACGCCGGTCATGCTGCGCGCCGAGAAGGAAGGCATCACGCCCAAGCAACTGATCGACCGCGTCTGGACCGAGCACAAG
CGCGACTTCGACAGCTTCCTGGTGTCGTTCGACAACTACTACAGCACCGACGCGGAGGAAAACCGCGAGCTGTGCGAAAA
GATCTACCTGGCCCTGAAGGCCGACGACCTGATCGCCGAACGCGAAGTCGAACAGTTCTACGACCCGGTCAAGAACATGT
TCCTGCCGGACCGCTTCATCAAGGGCGAGTGCCCGAAGTGCGGTGCAAAGGATCAGTACGGGGACTCGTGTGAAGTCTGC
GGCACCACCTATGTGCCGACCGACCTGAAGAATCCGTACTCGGTCGTCTCGGGCGCCACGCCGGTGCGCAAGTCGTCGAC
GCACTTCTTCTTCAAGCTGTCCGACCCGCGCTGCGAAACCTTCCTGCGCGAGTGGGTGGCCGATCTGGCCCAGCCCGAAG
CCGCCAACAAGATGCAGGAATGGCTCGGCAGCGAAGGCGAAGCCTCGACGCTGTCGGACTGGGACATCTCGCGCGACGCG
CCGTACTTCGGCTTCGAGATCCCGGGCGCACCGGGCAAGTACTTCTATGTGTGGCTGGACGCCCCGATCGGCTACTACGC
AAGCTTCAAGAACCTGGCCGAGAAGAAAGGCATCGACTTCGACGCATGGGTCGGCCCGCACTCCACGGCCGAGCAGTACC
ACTTCATCGGCAAGGACATCCTGTACTTCCACACGCTGTTCTGGCCGGCCATGCTGCGCTTCTCGGGCTACCGCACGCCG
ACCAACGTGTTCGCGCACGGCTTCCTGACCGTGGATGGCGCCAAGATGAGCAAGTCACGCGGCACGTTCATCACCGCGCA
GAGCTACATCGACACCGGCATGAACCCGGAATGGCTGCGCTACTATTTCGCCGCCAAGCTCAACGCGAGCATGGAAGACC
TGGACCTGAACCTGGACGACTTTATCGCTCGCGTCAACAGCGACCTGATCGGCAAGTACGTCAACATCGCGAGCCGCGCG
GCGGGCTTCCTGGTCAAGCGCTTCGAAGGCAAGGTCGACGAAGCCGCGCTGGCGCACCCGCTGCTCAAGCAACTGCGCGA
AGCCGCGCCGCAAGTCGCGCAGCTCTATGAAGCACGCGAGTACAGCAAGGCGCTGCGCCTGGTGATGGAGCTGACCGACA
CGGTCAATGCGTTCGTCGATACCGAAAAGCCGTGGGAACTGGCGAAGGATGAAGCCAAGCGCAGTGCGCTGCATGCCGCA
TGCTCGGTGTCGCTCGAGGCCTTCCGTCTGCTGACGGTCTATCTGAAGCCCGTGGTACCGACCGTGGCCGCCGGCGTGGA
GCGCTTCCTCAATGTCGAGCCGCTGGACTGGCGCGCGATCGACAAGCAGCTGTCGGCTTCCAGCCCGGTGCAGGCGTACC
AGCACCTGATGACGCGCGTCGATGCCAAGCAGGTCGATGCGCTGCTGGCCGCCAACCGGGAATCGCTGCAGGCGACGGCG
CCCGCCGCCGGCGCGGCAGCGGCCGCTTCGATCGAGCCGATCGCCGACACCATCACGATCGACGACTTCGCGAAGATCGA
TCTGCGGGTTGCCAAGATTGTGGAATGCCAGAAGGTCGAAGGCTCCAGCAAGTTGCTGCAGCTGACGCTGGACCTTGGCG
AAGGCCGTACGCGCAATGTGTTCTCCGGCATCCAGTCGGCCTACACGCCGGAGCAGCTTGTCGGCAAGCTGACCGTGGTG
GTTGCCAACCTGGCACCGCGCAAGATGAAGTTTGGCGTATCCGAAGGGATGGTGCTGGCTGCTTCGGCCGCTGATGAGAA
GGCCAATCCGGGGCTCTACATCCTGGAGCCGCACAGCGGTGCGGTGCCCGGCATGCGGATCGGCTGA

Upstream 100 bases:

>100_bases
GCGACGCGGTGCTTGCTAAAATAGCCCTTTCGCATTTCGCGCCCGGCTCAGCCGCACACGCGCGGGCGCACGCCTCCCGT
CACCACCACTGGCTCTTTTT

Downstream 100 bases:

>100_bases
TCTGCCTGCACGACAGGAAAAAAACGGCGGCGCCCTCCAGGCAGCCGCCGTTTTTCTTTATCGCCTCGGAGCTATCACCC
GCCCTGCATGCGAGAAGGAT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 688; Mature: 687

Protein sequence:

>688_residues
MTARRILVTSALPYANGQIHIGHLVEYIQTDIWVRFQRMMGNEVYYVGADDTHGTPVMLRAEKEGITPKQLIDRVWTEHK
RDFDSFLVSFDNYYSTDAEENRELCEKIYLALKADDLIAEREVEQFYDPVKNMFLPDRFIKGECPKCGAKDQYGDSCEVC
GTTYVPTDLKNPYSVVSGATPVRKSSTHFFFKLSDPRCETFLREWVADLAQPEAANKMQEWLGSEGEASTLSDWDISRDA
PYFGFEIPGAPGKYFYVWLDAPIGYYASFKNLAEKKGIDFDAWVGPHSTAEQYHFIGKDILYFHTLFWPAMLRFSGYRTP
TNVFAHGFLTVDGAKMSKSRGTFITAQSYIDTGMNPEWLRYYFAAKLNASMEDLDLNLDDFIARVNSDLIGKYVNIASRA
AGFLVKRFEGKVDEAALAHPLLKQLREAAPQVAQLYEAREYSKALRLVMELTDTVNAFVDTEKPWELAKDEAKRSALHAA
CSVSLEAFRLLTVYLKPVVPTVAAGVERFLNVEPLDWRAIDKQLSASSPVQAYQHLMTRVDAKQVDALLAANRESLQATA
PAAGAAAAASIEPIADTITIDDFAKIDLRVAKIVECQKVEGSSKLLQLTLDLGEGRTRNVFSGIQSAYTPEQLVGKLTVV
VANLAPRKMKFGVSEGMVLAASAADEKANPGLYILEPHSGAVPGMRIG

Sequences:

>Translated_688_residues
MTARRILVTSALPYANGQIHIGHLVEYIQTDIWVRFQRMMGNEVYYVGADDTHGTPVMLRAEKEGITPKQLIDRVWTEHK
RDFDSFLVSFDNYYSTDAEENRELCEKIYLALKADDLIAEREVEQFYDPVKNMFLPDRFIKGECPKCGAKDQYGDSCEVC
GTTYVPTDLKNPYSVVSGATPVRKSSTHFFFKLSDPRCETFLREWVADLAQPEAANKMQEWLGSEGEASTLSDWDISRDA
PYFGFEIPGAPGKYFYVWLDAPIGYYASFKNLAEKKGIDFDAWVGPHSTAEQYHFIGKDILYFHTLFWPAMLRFSGYRTP
TNVFAHGFLTVDGAKMSKSRGTFITAQSYIDTGMNPEWLRYYFAAKLNASMEDLDLNLDDFIARVNSDLIGKYVNIASRA
AGFLVKRFEGKVDEAALAHPLLKQLREAAPQVAQLYEAREYSKALRLVMELTDTVNAFVDTEKPWELAKDEAKRSALHAA
CSVSLEAFRLLTVYLKPVVPTVAAGVERFLNVEPLDWRAIDKQLSASSPVQAYQHLMTRVDAKQVDALLAANRESLQATA
PAAGAAAAASIEPIADTITIDDFAKIDLRVAKIVECQKVEGSSKLLQLTLDLGEGRTRNVFSGIQSAYTPEQLVGKLTVV
VANLAPRKMKFGVSEGMVLAASAADEKANPGLYILEPHSGAVPGMRIG
>Mature_687_residues
TARRILVTSALPYANGQIHIGHLVEYIQTDIWVRFQRMMGNEVYYVGADDTHGTPVMLRAEKEGITPKQLIDRVWTEHKR
DFDSFLVSFDNYYSTDAEENRELCEKIYLALKADDLIAEREVEQFYDPVKNMFLPDRFIKGECPKCGAKDQYGDSCEVCG
TTYVPTDLKNPYSVVSGATPVRKSSTHFFFKLSDPRCETFLREWVADLAQPEAANKMQEWLGSEGEASTLSDWDISRDAP
YFGFEIPGAPGKYFYVWLDAPIGYYASFKNLAEKKGIDFDAWVGPHSTAEQYHFIGKDILYFHTLFWPAMLRFSGYRTPT
NVFAHGFLTVDGAKMSKSRGTFITAQSYIDTGMNPEWLRYYFAAKLNASMEDLDLNLDDFIARVNSDLIGKYVNIASRAA
GFLVKRFEGKVDEAALAHPLLKQLREAAPQVAQLYEAREYSKALRLVMELTDTVNAFVDTEKPWELAKDEAKRSALHAAC
SVSLEAFRLLTVYLKPVVPTVAAGVERFLNVEPLDWRAIDKQLSASSPVQAYQHLMTRVDAKQVDALLAANRESLQATAP
AAGAAAAASIEPIADTITIDDFAKIDLRVAKIVECQKVEGSSKLLQLTLDLGEGRTRNVFSGIQSAYTPEQLVGKLTVVV
ANLAPRKMKFGVSEGMVLAASAADEKANPGLYILEPHSGAVPGMRIG

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI14043022, Length=524, Percent_Identity=28.8167938931298, Blast_Score=224, Evalue=3e-58,
Organism=Homo sapiens, GI24308436, Length=533, Percent_Identity=25.515947467167, Blast_Score=140, Evalue=3e-33,
Organism=Escherichia coli, GI1788432, Length=692, Percent_Identity=54.3352601156069, Blast_Score=753, Evalue=0.0,
Organism=Escherichia coli, GI1789455, Length=112, Percent_Identity=33.0357142857143, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17540794, Length=520, Percent_Identity=28.6538461538462, Blast_Score=236, Evalue=4e-62,
Organism=Caenorhabditis elegans, GI212645096, Length=529, Percent_Identity=24.007561436673, Blast_Score=145, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI71992781, Length=331, Percent_Identity=25.6797583081571, Blast_Score=110, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6321704, Length=557, Percent_Identity=27.1095152603232, Blast_Score=226, Evalue=8e-60,
Organism=Saccharomyces cerevisiae, GI6321610, Length=544, Percent_Identity=23.7132352941176, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24655311, Length=538, Percent_Identity=29.368029739777, Blast_Score=209, Evalue=5e-54,
Organism=Drosophila melanogaster, GI21358483, Length=521, Percent_Identity=25.9117082533589, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24651924, Length=103, Percent_Identity=37.8640776699029, Blast_Score=67, Evalue=5e-11,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 76734; Mature: 76603

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS50886 TRBD ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARRILVTSALPYANGQIHIGHLVEYIQTDIWVRFQRMMGNEVYYVGADDTHGTPVMLR
CCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCEEEE
AEKEGITPKQLIDRVWTEHKRDFDSFLVSFDNYYSTDAEENRELCEKIYLALKADDLIAE
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
REVEQFYDPVKNMFLPDRFIKGECPKCGAKDQYGDSCEVCGTTYVPTDLKNPYSVVSGAT
HHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCC
PVRKSSTHFFFKLSDPRCETFLREWVADLAQPEAANKMQEWLGSEGEASTLSDWDISRDA
CCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCC
PYFGFEIPGAPGKYFYVWLDAPIGYYASFKNLAEKKGIDFDAWVGPHSTAEQYHFIGKDI
CCCCEECCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
LYFHTLFWPAMLRFSGYRTPTNVFAHGFLTVDGAKMSKSRGTFITAQSYIDTGMNPEWLR
HHHHHHHHHHHHHHCCCCCCHHHHEEEEEEECCCCCCCCCCCEEEEHHHHCCCCCHHHHH
YYFAAKLNASMEDLDLNLDDFIARVNSDLIGKYVNIASRAAGFLVKRFEGKVDEAALAHP
HHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
LLKQLREAAPQVAQLYEAREYSKALRLVMELTDTVNAFVDTEKPWELAKDEAKRSALHAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
CSVSLEAFRLLTVYLKPVVPTVAAGVERFLNVEPLDWRAIDKQLSASSPVQAYQHLMTRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHH
DAKQVDALLAANRESLQATAPAAGAAAAASIEPIADTITIDDFAKIDLRVAKIVECQKVE
CHHHHHHHHHCCHHHHHCCCCCCCHHHHCCCCHHHHEEEECCHHHHHHHHHHHHHHHHCC
GSSKLLQLTLDLGEGRTRNVFSGIQSAYTPEQLVGKLTVVVANLAPRKMKFGVSEGMVLA
CCCEEEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHCCCCCCEEEE
ASAADEKANPGLYILEPHSGAVPGMRIG
EECCCCCCCCCEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
TARRILVTSALPYANGQIHIGHLVEYIQTDIWVRFQRMMGNEVYYVGADDTHGTPVMLR
CCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCEEEE
AEKEGITPKQLIDRVWTEHKRDFDSFLVSFDNYYSTDAEENRELCEKIYLALKADDLIAE
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
REVEQFYDPVKNMFLPDRFIKGECPKCGAKDQYGDSCEVCGTTYVPTDLKNPYSVVSGAT
HHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCC
PVRKSSTHFFFKLSDPRCETFLREWVADLAQPEAANKMQEWLGSEGEASTLSDWDISRDA
CCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCC
PYFGFEIPGAPGKYFYVWLDAPIGYYASFKNLAEKKGIDFDAWVGPHSTAEQYHFIGKDI
CCCCEECCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
LYFHTLFWPAMLRFSGYRTPTNVFAHGFLTVDGAKMSKSRGTFITAQSYIDTGMNPEWLR
HHHHHHHHHHHHHHCCCCCCHHHHEEEEEEECCCCCCCCCCCEEEEHHHHCCCCCHHHHH
YYFAAKLNASMEDLDLNLDDFIARVNSDLIGKYVNIASRAAGFLVKRFEGKVDEAALAHP
HHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
LLKQLREAAPQVAQLYEAREYSKALRLVMELTDTVNAFVDTEKPWELAKDEAKRSALHAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
CSVSLEAFRLLTVYLKPVVPTVAAGVERFLNVEPLDWRAIDKQLSASSPVQAYQHLMTRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHH
DAKQVDALLAANRESLQATAPAAGAAAAASIEPIADTITIDDFAKIDLRVAKIVECQKVE
CHHHHHHHHHCCHHHHHCCCCCCCHHHHCCCCHHHHEEEECCHHHHHHHHHHHHHHHHCC
GSSKLLQLTLDLGEGRTRNVFSGIQSAYTPEQLVGKLTVVVANLAPRKMKFGVSEGMVLA
CCCEEEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHCCCCCCEEEE
ASAADEKANPGLYILEPHSGAVPGMRIG
EECCCCCCCCCEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA